• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.37-37
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• 2018

We use two different methods for laboratory propagation from seed of lady's slipper orchid(Cypripedium macranthos Sw.); immature seed which also called green capsule or fully mature seed about 120~130 days from pollination. In green capsule culture, the seed pods should be collected within precisely right time. The right time of seed collection could be diverse under the wether conditions or nutritional factors of the plants. In fully matured seed culture, the more complicated procedures are needed to break the dormancy of the seed; thermal or chemical treatment. The seedlings in this study were easily germinated from immature seeds in Harvais medium; 53 days after pollination(DAP) in Cypripedium pubescens, DAP 65 in C. parviflorum and C. macranthos. The germinated seedlings were transplanted to hormone free media immediately to avoid abnormal growth of seedlings. When the seedlings have roots with a minimum length of around 2-3cm and have visible dormant buds, the seedlings were removed from the flask and stored in refrigerator for vernalization. To examine the correlation of seedlings and maternal plants, the 125 seedlings of C. macranthos were transplanted in the soil bed at a distance of 20-100 cm from mother plants on April 20. The survival rate of seedlings were 92% in 20 cm distance from the ripe plants, and 56 % in 100 cm distance. The seedlings which were transplanted near mother plants showed vigorous growth in plant height, leaf width, and especially dormant buds. Considering the existence of mycorrhiza which is a symbiotic association between a fungus and the roots of a orchid vascular, the various fungus from mother plants could affect the growth of the seedlings. These results indicate the possibility of high and stable production and practical industrialization of endangered lady's slipper orchids.

• Korean Journal of Weed Science
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• v.10 no.3
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• pp.186-191
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• 1990

This experiments was conducted to investigate the dormancy of Eleocharis kuroguwai tuber in the earth and the influence of seeding time and depth on the emergence of Eleocharis kuroguwai. The tuber of Eleocharis kuroguwai on underground was formed the most amount between 10cm and 15cm layer and formed the second a lot of tuber in the soil layer between 5cm and 10cm, the third between the surface of the earth and 5cm, the least between 15cm and 20cm. The total mean emergence of tuber was 78%, and the dormant tuber was approximately 22% of total tuber formed during a year in the earth. The distribution of dormant tuber under the ground was about 42% between 15cm and 20cm, 39% between 10 cm and 15cm, 14% between 5cm and 10cm, and 5% between the surface of the earth and 5cm. The dormancy percent of tuber formed at each soil layer was about 60% between 15cm and 20cm, 21% between 10cm and 15cm, 12% between 5cm and 10cm, and 7% between the surface of the earth and 5cm. The emergence days of the tuber seeded at low layer was short, and the emergence rate was high, The tuber was formed much more on seeding at May 30th than June 30th.

• Journal of Korean Society of Forest Science
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• v.40 no.1
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• pp.43-50
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• 1978

The elder berry was known to the rich in natural food colour resources and used in as for making wine, confectionary, perfumes, natural food colour, making elder berry juice, jelly, jam and medicinal properties or oils. In the present study, wish was to find the effect of various factors on the success of the vegetative propagation of elder berry by means cutting in the exposed field and green house was carried out and those obtained results can be summarized as follows. 1. Cuttings with dormant cutting stocs in the polyethylen house with heating and water mist spray resulting 100 percent survival. And temperature and the relative humidity in which fraim during the cutting season were around $20{\sim}25^{\circ}C$ and 70-90% respectively and in case as more significant other of the 1% Level. 2. With five varieties tested, resulting 93.8 percent survival, The F. value is not significant. 3. With four organs of cutting stock tested resulting 57.5 percent survival on the cuttings with two knodes of dormant cutting stock served as better cutting stock than others. The F. value is more significantly 1% Level. 4. Dormand bud served as possible cutting stock was found to be 17.66 survival percentage. 5. Both earlier and later stage of germinated Leaves with soft wood cutting stock poor cuttings, and the degree of development of 15th June cutting stock was optimum stage on the principal factor governing the success of cutting in the soft wood cutting showing 54% survival. The F. value is more 1% Level significant.

• Journal of Korean Society of Forest Science
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• v.62 no.1
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• pp.1-18
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• 1983

Sum of daily mean temperature, sum of daily mean relative humidity and sum of daily mean duration of sunshine during the growing season (March-October), and daily mean temperature, daily mean relative humidity and daily mean minimum temperature during the dormant season (November-February) were obtained respectively from the climactic data recorded at 26 different standard stations for 30 years from 1951 to 1980, to provide a method for proper selection of tree species suitable to a certain site. They were also marked on the map of Korea. The whole country was divided into 6 regions by trend of temperature variation and the regression equations for each region were produced to estimate the sum of daily mean temperature of the growing season and the sum of daily mean minimum temperature of the dormant season in a certain site where tree plantings are planned. The natural range of distribution of each species was expressed by the sum of daily mean temperature and daily mean minimum temperature on the basis of "Horizontal and vertical distribution of the Korean woody plants" reported by Chung and Lee (1965).

• Korean Journal of Weed Science
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• v.14 no.1
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• pp.49-55
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• 1994

A study was conducted to determine the effect of depth of burial, soil temperature and/or moisture on tuber sprouting of E, kuroguwai. Tubers were evenly distributed in the upper 30cm of soil. Tuber weight increased as depth of tuber formed increased. No dormancy in newly formed tubers was found, whereas mature tubers were dormant. When new tubers were subjected both to continuous $5^{\circ}C$ and to gradual decreasing temperatures regimes ranged from 20 to $1^{\circ}C$ for 30 days, the tubers remained non-dormant. Viability of tubers was reduced when buried at 5cm depth in dry and moist soil conditions, but no reduction was obtained when buried at 25cm depth, regardless of soil moisture conditions employed. Percent sprouting of tubers buried at 25cm depth increased with increasing duration of burial in three soil moisture conditions studied, wherease in dry and moist conditions percent sprouting of tubers buried at 25cm depth increased by 60-day burial and thereafter decreased. In submerged condition, tuber sprouting was greater when buried at 5cm depth than when buried at 25cm depth, and increased as duration of burial increased at the both depths.

• Horticultural Science & Technology
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• v.32 no.3
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• pp.296-302
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• 2014

The foliage of variegated Solomon's seal is excellent in cut flower arrangements. However, it has a restricted marketing period because the harvesting is limited in spring and summer. The increased interest requires the year-round production, thus techniques for dormancy breaking and forcing without low temperature treatment is needed. Therefore, experiments were conducted to d etermine whether gibberellic acid (GA) could break dormancy in variegated Solomon's seal. Thes prouting of dormant bud did not occur throughout the experiment when $GA_3$ $400mg{\cdot}L^{-1}$ was applied to dormant rhizomes as a soil drench. However, when plants were treated with a GA drench after scratch with razor blade or were given direct injection of GA, percent sprouting was increased up to 100 or 83.3%, respectively. However, because treatments with razor or syringe may damage internal organs, we tested another method, scarifying the rhizomes with sodium hypochlorite (NaOCl). Rhizome scarification with 4% NaOCl for 6 or 24 hours followed by drench of $GA_3$ $400mg{\cdot}L^{-1}$ increased the dormancy breaking percentage to 70 or 86.7%, respectively. Moreover, scarified and GA-treated rhizomes produced more leaves than untreated or GA-soil drenched plants in the glasshouse. These results showed the possibility of year-round production of variegated Solomon's seal foliage with rhizome scarification and GA treatments.

• Journal of Ginseng Research
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• v.23 no.3 s.55
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• pp.130-134
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• 1999

Somatic embryos were induced directly from cotyledon explants of Korean ginseng (Panax ginseng C.A. Meyer) on Murashige and Skoog (MS) medium with 2,4-D, BAP, kinetin or lacking growth regulators. When somatic embryos formed on all media grew to cotyledonary stage, the further development of embryos was ceased and remained in white color. By gibberellic acid (over 1.0 mg/1 $GA_3$) treatment, all the somatic embryos turned rapidly to green and germinated within 3 weeks. Chilling treatment also induced the germination of somatic embryos. The effective temperature regime was $-2^{\circ}C$ for over 8 weeks but more higher temperature than $0^{\circ}C$ did not effective for germination of somatic embryos. Ultrastructural observation revealed that the cotyledon cells of somatic embryos without chilling or $GA_3$ treatment contained numerous lipid reserves, dense cytoplasm, proplastids and non-activated mitochondria with poorly differentiated internal structure, but the cotyledon cells of germinating somatic embryos after chilling or $GA_3$ treatment highly vacuolated and contained well-developed chloroplasts and active state of mitochondria enclosing numerous cristae. The above results indicate that in vitro developed somatic embryos of Panax ginseng may be dormant after mature similar to zygotic embryos.

• FLOWER RESEARCH JOURNAL
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• v.16 no.2
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• pp.134-142
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• 2008

This work aims to obtain basic information for seed propagation of Hydrangea serrata for. acuminata. The germination percentage of the seeds taken on 15 November, 30 December, and 23 January was $90.0{\pm}4.16%$, $84.4{\pm}5.52%$, and $88.9{\pm}2.40%$, respectively. This suggest that seeds of Hydrangea serrata for. acuminata are non-dormant seeds. The optimum temperature for germination was $25^{\circ}C$ and light was necessary. Most of the growth parameters (shoot and leaf length, stem diameter, root length, no. of roots, T/R ratio, and fresh and dry wts.) were significantly greater at $25/20^{\circ}C$ and $25^{\circ}C$ than at the other temperatures. Low T/R ratio at relatively cool temperatures (15 and $20^{\circ}C$) was caused by suppressed top growth. In light quality treatment, red light (RL) significantly enhanced stem elongation. The greatest photosynthetic pigments (total chl, chl a/b, and carotenoid) were observed in seedlings grown in blue light (BL), followed by seedlings grown in RL+BL. When blue light was added, higher pigment contents were found. Effect of plug cell size (50, 72, 128, 162 and 200 cells) on the growth of seedlings was investigated. The highest top growth was observed in seedlings grown in 50 cell trays, followed by seedlings grown in 72, 128, 162, and 200 cell trays. However, there was no significant differences between 162 and 200 cell trays. Especially, smaller size leaves were observed in seedlings grown in smaller cell trays (lower volume and high plant density).

• Korean Journal of Plant Resources
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• v.27 no.2
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• pp.188-193
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• 2014

The sap outflow characteristics of Juglans sinensis and J. mandshurica were investigated to evaluate the optimum pruning period of walnut tree that there is a sap spill on dormant. The total period of sap outflow were 34 days for both J. sinensis and J. mandshurica. Total amount of sap outflow per tree in J. sinensis and J. mandshurica were 2,922 mL/tree and 3,135 mL/tree, respectively and the period of sap outflow and non sap outflow between two species were similar. ANOVA analysis showed that the amount of sap outflow was significant differences with day of sap outflow but there were no significant differences between species. From the correlation analysis between air-temperature and sap outflow, daily minimum temperature showed a positive correlation at the 1% level of significance (r=0.56 and r=0.46) for both J. sinensis and J. mandshurica. When branch of walnut tree that diameter is 5 cm cut on the period of sap outflow, the sap flowed down the longest period (48 days) but the sap outflow was not observed after budburst. Therefore, our study supported that the pruning have to avoid the period of sap outflow to reduce sap outflow of walnut tree.

• Journal of Ginseng Research
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• v.43 no.1
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• pp.38-48
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• 2019

Background: Interspecific ginseng hybrid, Panax ginseng ${\times}$ Panax quenquifolius (Pgq) has vigorous growth and produces larger roots than its parents. However, F1 progenies are complete male sterile. Plant tissue culture technology can circumvent the issue and propagate the hybrid. Methods: Murashige and Skoog (MS) medium with different concentrations (0, 2, 4, and 6 mg/L) of 2,4-dichlorophenoxyacetic acid (2,4-D) was used for callus induction and somatic embryogenesis (SE). The embryos, after culturing on $GA_3$ supplemented medium, were transferred to hormone free 1/2 Schenk and Hildebrandt (SH) medium. The developed taproots with dormant buds were treated with $GA_3$ to break the bud dormancy, and transferred to soil. Hybrid Pgq plants were verified by random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analyses and by LC-IT-TOF-MS. Results: We conducted a comparative study of somatic embryogenesis (SE) in Pgq and its parents, and attempted to establish the soil transfer of in vitro propagated Pgq tap roots. The Pgq explants showed higher rate of embryogenesis (~56% at 2 mg/L 2,4-D concentration) as well as higher number of embryos per explants (~7 at the same 2,4-D concentration) compared to its either parents. The germinated embryos, after culturing on $GA_3$ supplemented medium, were transferred to hormone free 1/2 SH medium to support the continued growth and kept until nutrient depletion induced senescence (NuDIS) of leaf defoliation occurred (4 months). By that time, thickened tap roots with well-developed lateral roots and dormant buds were obtained. All Pgq tap roots pretreated with 20 mg/L $GA_3$ for at least a week produced new shoots after soil transfer. We selected the discriminatory RAPD and ISSR markers to find the interspecific ginseng hybrid among its parents. The $F_1$ hybrid (Pgq) contained species specific 2 ginsenosides (ginsenoside Rf in P. ginseng and pseudoginsenosides $F_{11}$ in P. quinquefolius), and higher amount of other ginsenosides than its parents. Conclusion: Micropropagation of interspecific hybrid ginseng can give an opportunity for continuous production of plants.