• BMB Reports
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• v.40 no.6
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• pp.921-927
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• 2007

UCP2 and UCP3 are members of the uncoupling protein family, which may play roles in energy homeostasis. In order to determine the regulation of the predominant expression of UCP3 in skeletal muscle, the effects of differentiation and myogenic regulatory factors on the promoter activities of the mouse UCP2 and UCP3 genes were studied. Reporter plasmids, containing approximately 3 kb of the 5'-upstream region of the mouse UCP2 and UCP3 genes, were transfected into C2C12 myoblasts, which were then induced to differentiate. Differentiation positively induced the reporter expression about 20-fold via the UCP3 promoter, but by only 2-fold via the UCP2 promoter. C2C12 myoblasts were cotransfected with expression vectors for myogenin and/or MyoD as well as reporter constructs. The simultaneous expression of myogenin and MyoD caused an additional 20-fold increase in the reporter expression via the UCP3 promoter, but only a weak effect via the UCP2 promoter. In L6 myoblasts, only MyoD activated the UCP3 promoter, but in 3T3-L1 cells neither factor activated the UCP3 promoter, indicating that additional cofactors are required, which are present only in C2C12 myoblasts. The expression of UCP2 and UCP3 is differentially regulated during muscle differentiation due to the different responsiveness of their promoter regions to myogenin and MyoD.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.38 no.3
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• pp.75-82
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• 2020

Heungseon Daewonwang Lee Ha-eung was the father of King Gojong, who appointed as Daewongun in December 1863. On February 2, 1898, after the death of Heungseon Daewongun, he was buried in the Asodang in Gongdeok-dong, Mapo-gu, where he normally enjoyed. On August 24, 1907, he was promoted to Daewonwang. After being promoted to Daewonwang, the Asodang tomb was relocated to Daedeok-dong, Paju, and the tomb was promoted to Won. In the Wonchim space promoted to the facilities suitable for the original were created, and through this, it was possible to analyze the characteristics of the Wonchim and the Wonchim space of the tomb on the Heungwon. In addition, by comparing and analyzing the spatial composition between Myo and Won, it was possible to derive the spatial composition characteristics of the Joseon Dynasty Won(園), and Myo(墓). Looking at the research results derived through the analysis of 『Heungwon Cheonbong Registration』, tomb of Heungseon Daewongun, located in Asodang, Mapo-gu, was promoted to Heungseon Daewonwang, and was relocated as Jangneung in Uncheon-ri, Paju, the former tomb of King Injo. The promoted of tomb was also promoted from Myo to Won. In addition, the storehouses and facilities used in Mapo Asodang were moved to Uncheon-ri, Paju, and reused. Newly constructed monuments, Biseog, Chimgak, Jemulgo, Subokcheong, Hongsalmun, and Wells according to the promoted of the Won. It was confirmed that there is a difference in the spatial composition of Won and Myo when looking at the difference in the composition of the ornament objects and the facility.

• The Korea Journal of Herbology
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• v.23 no.2
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• pp.159-168
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• 2008

Objectives : The present study has been undertaken to investigate the effects of Dipsaci Radix on Muscle Fiber Atrophy and MyoD Expression in Gastrocnemius of MCAO Rats Methods : In order to investigate effects of Dipsaci radix on the skeletal muscle atrophy following stroke, cerebral infarct was induced by the middle cerebral artery occlusion (MCAO) in the rats. Water extract of Dipsaci radix (184.4 mg/100 g) was treated for 4 weeks, once a day orally, after the MCAO. Effects were evaluated with muscle fiber type composition and cross-sectioned area of muscle fibers in gastrocnemius of the unaffected & affected hind limbs. And MyoD protein expression in gastrocnemius was demonstrated with immunohistochemistry and western blotting. Results : Obtained results were as follows; 1. Infarct volume was not attenuated by Dipsaci radix treatment in the MCAO rats. 2. At the affected-side hind limb of the MCAO rats, the increase of type-I fibers and the decrease of type-II fibers were induced by Dipsaci radix treatment. 3. At the affected-side hind limb of the MCAO rats, decreases of cross-sectioned areas of type-I and type-II fibers were attenuated by Dipsaci radix treatment. 4. At the affected-side hind limb of the MCAO rats, MyoD positive cells were increased by Dipsaci radix treatment. 5. At the affected-side hind limb of the MCAO rats, MyoD expressions were increased by Dipsaci radix treatment. Conclusions : These results suggest that Dipsaci radix has a protective effect against muscle atrophy, through the inhibition of the muscle cell apoptosis, following the central nervous system demage.

• The Journal of Churna Manual Medicine for Spine and Nerves
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• v.4 no.2
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• pp.21-29
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• 2009

Objectives : The purpose of this study is to find out the effects of myofascial releasement on nuchal pain caused by traffic accidents. Methods : The 30 patients were divided into 2 groups : group A was treated except myofascial releasement and group B was treated with myofascial releasement. Both group were treated with acupuncture treatment, physical theraphy and herbal medication. Patients were evaluated by McGill Pain Questionnaire-Short Form(SF-MPQ), Visual Analogue Scale(VAS), Pain Disability Index(PDI) and Neck Disability Index(NDI). Results : 1. Both Group were significantly decreased in affective of SF-MPQ, VAS, after 10 times of treatment. 2. Group B compared with the Group A was significantly decreased in affective of SF-MPQ, NDI and PDI after 10 times of treatment. Conclusions : We found out that myofascial releasement is considered to be effective and useful on nuchal pain caused by traffic accidents.

• Journal of Korean Medicine Rehabilitation
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• v.19 no.4
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• pp.19-35
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• 2009

Objectives : This experimental study was designed to investigate the effect of KangwhalSokdan-tang(Jianghuoxuduan-tang) on the muscle regeneration of atrophied rat muscle by hindlimb suspension. Materials and methods : In this study, Sprague-Dawley rats weighing about 250g were subjected to hindlimb suspension and divided into total four groups: Normal group(n=6), Control group(n=6), Hindlimb non-treatment group(n=6), Hindlimb treatment group(n=6). Experiments were seperately tried two times. The first trial was studied by the following two groups; The first was normal group(n=6). The second was group(n=18) for hindlimb suspension during 2 weeks (control I group). The second trial after 2 weeks hindlimb suspension was studied by the following three groups; The third group(n=6) was expired immediately after 2 weeks hindlimb suspension. The forth group(n=6) was given free activity during 2 weeks after 2 weeks hindlimb suspension. The fifth group(n=6) was administrated of KST during 2 weeks after 2 weeks hindlimb suspension. In order to investigate degree of muscle atrophy, body weight and gastrocnemius muscle mass were compared. To analyze muscle regeneration factors(expression of IGF-1, Myogenin, MyoD), Western blot was used. Results : The results were analyzed by statistical process as follows, 1. In body weight, all hindlimb suspension groups were lower than normal group, but tendency of increase was shown in KST group compared to non-treatment group after 2 weeks hindlimb suspension. 2. In gastrocnemius muscle mass, KST group on both side was significantly higher than non-treatment group after 2 weeks hindlimb suspension. 3. In case of IGF-I, Type I of KST group was significantly increased than non-treatment group, but Type II was not shown significance. 4. There was no significantly difference in Myogenin. 5. In MyoD, Type I of KST group was significantly increased than control group, and Type II of KST group was significantly increased than non-treatment group. Conclusions : In summary, this study demonstrates that KST administration has an effect to prevent muscle atrophy and contribute muscle regeneration and proliferation. And also it is suggested that IGF-I and MyoD is major factors of myogenesis expression to KST adminstration after hindlimb suspension.

• 대한생식의학회:학술대회논문집
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• 2001.11a
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• pp.92-92
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• 2001

• 대한생식의학회:학술대회논문집
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• 2001.06a
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• pp.44-45
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• 2001

• 대한생식의학회:학술대회논문집
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• 2001.06a
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• pp.58.2-59
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• 2001

• 대한생식의학회:학술대회논문집
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• 1999.11a
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• pp.43-44
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• 1999

• 대한생식의학회:학술대회논문집
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• 1999.11a
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• pp.104.1-104.1
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• 1999