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Molecular Detection of Giardia intestinalis from Stray Dogs in Animal Shelters of Gyeongsangbuk-do (Province) and Daejeon, Korea

  • Shin, Jin-Cheol;Reyes, Alisha Wehdnesday Bernardo;Kim, Sang-Hun;Kim, Suk;Park, Hyung-Jin;Seo, Kyoung-Won;Song, Kun-Ho
    • Parasites, Hosts and Diseases
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    • v.53 no.4
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    • pp.477-481
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    • 2015
  • Giardia is a major public health concern and considered as reemerging in industrialized countries. The present study investigated the prevalence of giardiosis in 202 sheltered dogs using PCR. The infection rate was 33.2% (67/202); Gyeongsangbuk-do and Daejeon showed 25.7% (39/152, P<0.0001) and 56% (28/50), respectively. The prevalence of infected female dogs (46.7%, P<0.001) was higher than in male dogs (21.8%). A higher prevalence (43.5%, P<0.0001) was observed in mixed breed dogs than purebred (14.1%). Although most of the fecal samples collected were from dogs of ${\geq}1$ year of age which showed only 27.4% positive rate, 61.8% (P<0.001) of the total samples collected from young animals (<1 year of age) were positive for G. intestinalis. A significantly higher prevalence in symptomatic dogs (60.8%, P<0.0001) was observed than in asymptomatic dogs (23.8%). Furthermore, the analysis of nucleotide sequences of the samples revealed that G. intestinalis Assemblages A and C were found in the feces of dogs from Gyeongsangbuk-do and Daejeon. Since G. intestinalis Assemblage A has been known to infect humans, our results suggest that dogs can act as an important reservoir of giardiosis in Korea. Hence, hygienic management should be given to prevent possible transmission to humans.

Design of the anvil shape in sizing press for decrease of the defect generated width reduction (사이징 프레스에서 폭 압하 공정중 결함 감소를 위한 엔빌의 형상설계)

  • Lee S.H.;Kim D.H.;Byon S.M.;Park H.D.;Kim B.M.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2006.05a
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    • pp.437-438
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    • 2006
  • Generally, the vertical roll process is used to achieve extensive width reduction in hot strip mill. However, it is difficult to avoid the defects such as dog-bone and seam-defect. The sizing press has been developed in response to the defects mentioned above. Especially, this study is carried out to investigate the deformation of slab by two-step sizing press. The deformation behavior in the width sizing process is more favorable than that in conventional vertical rolling edger. The objective of this study is to determine the optimal anvil shape parameters in the sizing press with two-step die from the viewpoint of edge-seam length. In general, the edge-seam defect occurs parallel to the rolling direction at both edges in horizontal rolling process after sizing press. The optimal combination of the parameters is determined by FE-simulation and Artificial Neural Network (ANN). The slab deformation in sizing press with convex anvil is analyzed by FE-simulation. The most suitable profile of the anvil is also discussed fur the improvement of trimming loss because of the side seam defect by FE-simulation and ANN.

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Serosurvey for antibodies against Neospora caninum in farmed deer and wild water deer in Jeonbuk province (사육사슴 및 야생고라니의 Neospora caninum에 대한 항체가 조사)

  • Jo, Young-Suk;Lee, Jeong-Won;Chung, Yun-Shin;So, Seung-Young;Park, Hyun-Jong;Cho, Ho-Seong;Kim, Bum-Seok;Lim, Chae-Woong
    • Korean Journal of Veterinary Service
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    • v.33 no.3
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    • pp.271-274
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    • 2010
  • With deer farming now becoming recognized as one of the alternative livestock farming practices, there has been much attention on infectious diseases of cattle, but less awareness of these diseases in deer. Serum samples were randomly collected from 78 deer from 31 farms and 7 wild water deer from wild animal medical center during 2005 to 2007, respectively. A total 85 sera were tested for Neospora caninum antibodies by ELISA. Fourteen farmed deer(17.9%) were positive to N. caninum but no antibodies were found in sera from wild water deer. The sera from all of the area for these study showed positive in the range from 6.7 to 30.7%. On the basis of these study, farmed deer in Jeonbuk province were exposed extensively and seriously to N. caninum which might present a risk to other species included cattle and dog.

The effect of acupuncture at BL-23 and BL-28 on function of the urinary bladder in dogs (개에서 신수(BL-23) 및 방광수(BL-28)에 대한 침술이 방광기능에 미치는 영향)

  • Park, Hong-Sik;Yun, Young-Min;Lee, Kyoung-Kap
    • Korean Journal of Veterinary Research
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    • v.47 no.4
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    • pp.469-473
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    • 2007
  • This study was performed to investigate the effects of urinary acupoint (BL-23, BL-28) by acupuncture (AP) and electroacupuncture (EA) on urinary bladder in dogs. Four healthy male dogs aged within the range of 6~8 months were used in this experiment. For Electrodes implantation on the bladder, dogs were to undergo surgical operation. The purpose of investigating the effect on urinary bladder was to evaluate electromyogram (EMG) changes according to AP or EA at Shen Shu (BL-23), Pang Guang Shu (BL-28) and non-acupoint. AP and EA (2~4 V, 10 Hz) were applied for 20 min to each point. EMG was evaluated when acupuncture began stimulation and as soon as the stimulation by electroacupuncture was gone. By the experiment of investigating the effect of urinary time it was estimated to measure time up to urination from stimulation on effective acupoint, after saline was filled with 70% of whole volumes on urinary bladder. The wave of EMG on resting stage showed simple and regular, whereas that of EMG on urination showed irregularly strong peaks before urination. Acupuncture of BL-28 had an influence on changes of EMG which had irregular peaks on urination. But the changes of EMG after acupuncture of BL-28 and control were regular like resting stage. The changes of EMG after EA at BL-23 and BL-28 showed irregularly a variety of wave forms. The interval through urination from stimulation at BL-28 and control become short as measuring time by EA (p<0.01). According to the results, AP at BL-28 was effective to urinary bladder. EA at BL-23 and BL-28 was effective to urinary bladder. Especially, the interval on stimulating at BL-28 by EA was the shortest in measuring time to urination from stimulation.

Effect of Sex and Obese Index on Breakfast and Snack Intake in Elementary School Students (초등학생의 성별과 비만도가 아침 식사와 간식 섭취에 미치는 영향)

  • Joo, Eun-Jung;Park, Eun-Sook
    • Journal of the Korean Society of Food Culture
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    • v.13 no.5
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    • pp.487-496
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    • 1998
  • The survey was conducted in July first to 15th, among 463 elementary school students, 4th grade to 6th grade in Chonj. The subjects were 252 male and 211 female students. The mean age of the subjects was 9.8 year The percent of relative body weight of the subjects compared to Korean standard was 100.1%, it almost matched to Korean standard. The percent of relative body weight in male students was 100.4%, where as the female's was 99.7%. Thirty six percent of the subjects have breakfast irregularly. The reason skipping breakfast were 'no appetite' 50.5%, 'get up late' 35.8%,' no delicious food' 18.2%. The reason skipping breakfast was significantly different by sex and obese index. The frequency taking snack was significantly different by sex, 34.6% of female subjects took snack more than twice per day, while 16.7% of male subjects did it. The most frequently taking snack were fruits, the points was 4.0 of 5.0. Ramyun, Sundai were taking more frequently among males than females. Females took fruits more frequently than males. Hot dog, ice cream ,and chocolate intakes were significantly different by obese index. The most preference of snack were fruits and ice cream in all subjects. The preference of ramyun, hamburger, egg, chicken, dried squid, milk, peanut, and soft drink were higher in males than in females. Biscuit, ice cream, and chocolate preference were significantly different in obese index.

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Diagnostic Laparoscopic Biopsy in Dogs and Cats (개와 고양이에서 복강경을 이용한 생검 증례)

  • Park, Se-Jin;Lee, Seung-Yong;Jin, So-Young;Kim, Min-Hyang;Seok, Seong-Hoon;Kim, Young-Ki;Lee, Hee-Chun;Jung, Dong-In;Hong, Il-Hwa;Yeon, Seong-Chan
    • Journal of Veterinary Clinics
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    • v.33 no.1
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    • pp.21-24
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    • 2016
  • Four dogs and two cats received laparoscopic or laparoscopic-assisted biopsy of abdominal organs for definite diagnosis of illness. Patients were presented with a chief complaint of acute or chronic vomiting excluding case 5 (seizure). Two patients needed the liver biopsy (case 1 and 5), and others did stomach and small intestine biopsy excluding case 2 (small intestine biopsy only). Surgical procedure was performed with modified standard methods. All patients recovered uneventfully and did not require any hospitalization without complications. Laparoscopic and laparoscopic-assisted biopsy could offer benefits of reduced incision length and rapid postoperative recovery.

Evaluation of Azygos Vein to Aorta Ratio using Multi-Detector Computed Tomography in Dogs

  • Park, Hyunyoung;Kim, Jungyong;Kim, Soochan;Jeong, Woochang;Lee, Jungwoo;Choi, Sooyoung;Choi, Hojung;Lee, Youngwon
    • Journal of Veterinary Clinics
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    • v.37 no.2
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    • pp.82-87
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    • 2020
  • The purpose of this study is to evaluate the azygos vein to aorta diameter ratio (AV/AO ratio) in healthy dogs and dogs with diseases that may cause azygos vein dilation. The normal groups included eleven healthy beagle dogs and eleven small breed dogs. The abnormal group included 43 dogs that had diseases with high right atrial pressure (RA group), obstruction of cranial or caudal vena cava (CVC group) and porto-azygos shunt group (PSS group). The diameter of AV and AO were measured at three sites on thoracic CT images; the level of the cranial endplate of 12th thoracic vertebra (TV level), the tracheal bifurcation (TB level) and the insertion to the cranial vena cava (CrVC level). The AV/AO ratio at the CrVC level had significantly higher values than those at the TV and TB levels in the normal groups. The AV/AO ratios of TV and TB levels in the CVC group and the values at all measurement sites of the PSS group were significantly higher than the normal groups. AV/AO ratio will be a useful factor for the evaluation of various clinical conditions that change the AV diameter with respect independent to the body weight in dogs.

Prevalence on protective serum antibodies of canine influenzae virus in Ulsan area (울산지역의 개 인플루엔자 바이러스의 항체보유 실태 조사)

  • Sung, Ki-Chang;Lee, Eun-Woo;Park, Chang-Eun
    • Korean Journal of Veterinary Service
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    • v.36 no.4
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    • pp.333-340
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    • 2013
  • Canine influenza virus (CIV) is an emerging pathogen that causes severe and acute respiratory disease in dogs. In 2006, the H3N2 CIV was first identified in dogs from Guangdong province in China. The nine isolates were grouped together with the canine H3N2 viruses isolated from dogs and cats in Korea. The possible interspecies transmission of influenza A virus is very important. We carried out a serological retrospective study using invited canine serum. The hospital invited 123 dogs, first vaccination group were revealed with CIV antibody positive rate of 81.8%. the second vaccination group were detected a positive rate of 91.2%. Antibody generation rate was higher in 3~10 years dogs. Protective antibody titers were detected from 2 weeks to 12 months. thereafter below the protective antibody. The results indicate that H3N2 CIV may have been consistently circulating in dog populations. Recently. These findings showed that H3N2 CIV has the capacity to replicate in and transmit among cohoused dogs and underscore the need for continued public health surveillance. Considering the result continuous management and prevention system against CIV is required at the concentrated animal care centers. The importance of CIV surveillance in this region for understanding the genesis of this virus, and it is important to remain aware of the potential of H3N2 CIV to be transmitted from dogs to the human population.

Seroepidemiological survey on canine distemper, canine parvovirus, canine coronavirus, caninie adenovirus type-2, canine parainfluenzavirus of dogs by indirect immunofluorescent test (간접형광항체법(間接螢光抗體法)에 의한 개의 바이러스-canine distemper virus, canine parvovirus, canine coronavirus, canine adenovirus type-2, canine parainfluenzavirus-항체분포 조사)

  • Yoon, Ki-bok;Kang, Mun-il;Park, Nam-yong;Han, Dong-un
    • Korean Journal of Veterinary Research
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    • v.35 no.1
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    • pp.75-85
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    • 1995
  • An indirect immunofluorescent antibody test was applied to survey the antibody prevalence on five canine viruses including canine distempervirus(CDV), canine parvovirus(CPV), canine coronavirus(CCV), canine adenovirus type-2(CAV-2), canine parainfluenzavirus(CPIV) in dogs. The period studied was from October 1992 to June 1993. A total of 80 dog sera was collected from veterinary clinics in Kwangju and Seoul, and examined for the presence of virus antibodies. Immunofluorescent antibodies(IFA) to all viruses were present in a high percentage of 80 sera tested. Seventyfive(93.8%) showed detectable IFA against CPV, 67(83.8%) against CDV, 51(63.8%) against CCV, 42(52.5%) against CPIV and 34(42.5%) against CAV-2. These suggested that all viruses were endemic in the communities. IFA levels against each virus were also distributed fairly irregularly. IFAs for CDV and CPV were detected more frequently with a relatively high incidence in vaccinated group less than 1 years of age. IFAs for CAV-2 were detected more frequently with growing age. In the correlation of clinical signs and antibody prevalence, dogs that showed hematochezia and vomiting had high titers in the positive sera is noteworthy, particularly for CDV and CPV. The significance between dogs those who had diarrhea, dyspnea and salivation and those viruses were obscure.

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Ultrasonographic appearance of the gestational structures throughout accurately timed pregnancy in Korea Jin-do dogs I. Time of initial detection of the fetal and extra-fetal structures (진도개에서 임신일령에 따른 임신구조물의 초음파상 I. 태아 및 태아외구조물의 최초 관찰시기)

  • Kang, Byong-kyu;Son, Chang-ho;Shin, Chang-rok;Choi, Han-sun;Oh, Ki-seok;Park, In-cheul
    • Korean Journal of Veterinary Research
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    • v.36 no.1
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    • pp.235-245
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    • 1996
  • Serial ultrasonographic examinations were performed on 9 pregnant Korea Jin-do bitches that were the Korean native breed, from days 15 to 60 pregnancy to determine the time of first detection and ultrasonographic appearance of the fetal and extra-fetal structures of pregnancy. Gestational age was timed from the day of ovulation (Day 0), which was estimated to occur when plasma progesterone concentration was first increased above 4.0 ng/ml. Gestational ages at earliest detection of the following fetal and extra-fetal structures were; gestational sac at days 17 to 22; placental layers in the uterine wall at days 20 to 24; zonary placenta at days 25 to 28; yolk sac membrane at days 22 to 24; amnionic membrane at days 27 to 29; embryo initial detection at days 21 to 23; fetal heartbeat at days 21 m 25; bipolar shape embryo at days 25 to 26; fetal movement at days 28 to 31; limb buds at days 31 to 35; anechoic area in head at days 31 to 36; stomach at days 34 to 37; urinary bladder at days 34 to 37; skeleton at days 36 to 38; dorsal sagittal tubular structure in vertebrae at days 36 to 38; lung hyperechoic vs liver at days 37 to 39; liver hypoechoic vs abdomen at days 37 to 40 and kidney at days 43 to 48, respectively.

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