• Journal of Microbiology
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• 제42권3호
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• pp.216-222
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• 2004

In a previous paper, the ogdH gene that encodes 2-oxoglutarat dehydrogenase was isolated from Salmonella typhimurium. The catalytic N-terminal region in the enzyme was found to be very specific for the Salmonella species. Therefore, the aim of the present study was to detect S. typhimurium in food sources using primers designed for OGDH-l and OGDH-2 which were based on the salmonella-specific region of the ogdH gene. A simple polymerase chain reaction (PCR) detection method was developed to detect low numbers of S. typhimurium in a chicken meat microbial consortium. Using the ogdH-specific primers under stringent amplification conditions and for gene probe analysis, fewer than 100 colony-forming units (CFUs) were detectable when pure cultures were employed. When the PCR assay was run on S. typhimurium-contaminated meat contents, only the positive meat samples containing as few as 200 CFUs reacted to the assay. The method employed for sample processing is simple and it was determined to provide a sensitive means of detecting trace amounts of S. typhimurium-specific sequences in the presence of mixed meat microbial populations. When compared with six representative intestinal gram-negative bacterial strains in foods, including Vibrio parahaemolyticus, V. vulnificus, Enterobacter cloacae, E. coli O157:H7, Pseudomonas aeruginosa, and Proteus sp., S. typhimurium had a unique and distinct PCR product (796 bp). In conclusion, the two OGDH primers were found to be rapid and sensitive detectors of Salmonella spp for the PCR method.

• 대한원격탐사학회지
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• 제32권6호
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• pp.673-679
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• 2016

본 연구에서는 Geostationary Ocean Color Imager (GOCI)의 수출광량(Water-leaving radiance) 자료와 흡광계수(Absorption Coefficients) 자료를 이용하여 한국 남해에 발생한 Cochlodinium polykrikoides 적조를 탐지하는 방법을 제안한다. C. polykrikoides의 분광스펙트럼을 분석하여, 412 nm에서 555 nm구간 파장대의 수출광 및 흡광 특성을 확인하였고 이를 이용하여 적조지수를 개발하였다. 본 연구에서 제안한 탐지기법은 광학적으로 복잡한 남해에서 발생한 적조를 탐지하였으며, 이러한 결과를 바탕으로 향후 적조 방재에 활용될 수 있을 것이라 판단된다.

• 한국IT서비스학회지
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• 제10권1호
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• pp.105-116
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• 2011

With recent spotlight on the, uniquitous computing technology, the need for object of indentification and location infrastructure has increased. Such GPS technolgy must utilize IEEE 802.15.4 Zigbee used for existing wireless sensor network infra as a basice element for user's context-awareness in a uniquitous environement, for effectiveness.Such real-time GPS service is provided in the internal environment where the user would actually are and most high-rise buildlings apply. Underthe assumption, the real-time GPS technology is seperated by each floor, and signals do not get transmitted to other floors, the application on one floor within the high-rise buildling was conducted. This study intends to suggest a floor detection algorithm using IEE 802.15.3/Zigbee's RSSI which supports the accuracy within a couple of meters for the user's the movement between the floors in high-rise buildings in a complex environment. It proposes an floor detection algorithm using IEEE 802.15.4/Zigbee's RSSI which provides accuracy within a radius of few meters for the users movement between the floors for real-time location tracking within high-rise building in a cmoplex environment. Furthermore, for more accurate real-time location tracking, it suggests an algorithm for real-time location tracking using IEEE 802.15.4a/Zigbee's CSS technology based on triangulation. Based on the suggested algorithm, it designs a hybrid real-time location tracking service system in a high-rise buildling and test its functions.

• 정보보호학회논문지
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• 제29권5호
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• pp.1019-1026
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• 2019

지속적으로 새롭게 등장하는 악성 파일(malware)탐지의 어려움으로 인해 머신러닝 기반 인공지능 백신 개발의 중요성이 크게 대두되고 있다. 하지만 현존하는 인공지능 백신은 파일의 일부 영역만을 검사하기 때문에 탐지율이 떨어진다는 단점이 존재한다. 이에 본 논문에서는 독자적인 로직을 기반으로 개발한 인공지능 백신에 근거하여, 파일 내 전체 데이터를 검사하는 방법을 제안한다. 그 중 정상 파일과 비교했을 때 악성 파일에만 존재하는 unique한 함수에서 추출한 OP Code 특징을 학습 데이터셋으로 한 진단법 강화 방안을 제시한다. 해당 강화법의 성능을 Random Forest 알고리즘을 기반으로 한 CSV 데이터셋 학습과 Inception V3 모델을 기반으로 한 이미지 데이터셋 학습으로 나누어 테스트해본 결과, 약 80%의 탐지율을 도출하는 것을 확인할 수 있었다.

• 대한골대사학회지
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• 제25권4호
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• pp.235-241
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• 2018

Background: Procollagen type I N-terminal propeptide (PINP) is one of the most clinically useful bone formation biomarkers. Therefore, the purpose of this study was to independently evaluate the performance of automated total PINP assay and established age- and gender- specific reference intervals for PINP in healthy Korean population. Methods: The imprecision, linearity, and detection capability of Elecsys total PINP assay was determined and reference interval was established using 599 serums from Korean population with normal bone mineral densities based on bone densitometry. Age groups were divided into 20s, 30s, 40s, 50s, 60s and over. Results: Elecsys total PINP had excellent performance in imprecision, linearity, and detection capability. When partitioning age groups in Korean male and female populations, there was significant difference in total PINP between different age groups. In male populations, PINP level was decreased with increasing age, then it remained steady after middle-age. In female populations, there was a decreasing tendency similar to that in the male population with a sharp increase in the 50 to 59 age group. Conclusions: Elecsys total PINP assay showed precise and reliable performance in our study. We established age-related PINP reference intervals for Korean male and female population with normal bone mineral densities.

• 한국동물위생학회지
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• 제34권4호
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• pp.417-428
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• 2011

Species-specific PCR assay was developed for detection of cattle, sheep, goat, horse, dog, pig, chicken, duck, goose, and turkey using mitochondrial 12S rRNA and 16S rRNA as target genes. Also, an internal positive control was used to detect possible false negatives by using 18S rRNA gene. We designed species-specific primers with amplicon length of 190, 219, 350, 467, 241, 119, 171, 229, 111 and 268 bp for cattle, sheep, goat, horse, dog, pig, chicken, duck, goose, and turkey respectively. The specificity of the primers was tested against the other 10 non-target animal species and a cross-reaction was not observed. We developed two multiplex PCR assays for the simultaneous identification of Korea's major livestock species (cattle, pig, chicken and duck) and poultry species (chicken, duck, goose and turkey) from analogous samples, retaining the same specificity. The limit of detection of the multiplex PCR assay (cattle, pig, chicken and duck) ranged between 1 pg and 0.1 pg of template DNA extracts from raw meat. Applying multiplex PCR assays to DNA extracts from experimental pork/beef and pork/chicken tested raw and heat-treated ($120^{\circ}C$ for 30 min) mixtures respectively, detection limit was 0.1% level beef in pork, pork in beef and chicken in pork and 1.0% level pork in chicken. In conclusion, this assay using gel-based capillary electrophoresis would be very useful in highly sensitive and rapid identification of animal species or ingredients in minced meat and other meat products.

• Journal of Genetic Medicine
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• 제5권1호
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• pp.7-14
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• 2008

Twenty years have passed since a prenatal screening for Down syndrome and neural tube defect was applied to obstetric field. The Quad test (AFP, hCG, uE3, Inhibin-A) of the second trimester and the combination test (PAPP-A, hCG, NT) of the first trimester became popular now. The recent trend of prenatal screening is to combine these two screening tests together in order to increase a detection rate of Down syndrome. Three types of screening methods are introduced as follows; integrated test, sequential test and contingent test. In addition to combination of each test, an incorporation of characteristic ultrasound findings of Down syndrome is suggested for its risk calculation. The absence of fetal nasal bone would be a very useful marker especially in the first trimester screening test. According to a change of way calculating risk of Down syndrome, obstetrician's role will be more increased not by passive participation, but by active participation using ultrasound in risk calculation.

• 센서학회지
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• 제15권6호
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• pp.442-448
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• 2006

Ultrasonic anemometers using pulse envelope detection-based method are standard instruments in most meteorological studies. In this paper, a new phase measurement method is tried to achieve the enhanced resolution without changing dimensions. The measurement sensitivity, dynamic range, and measurement speed of the new instrument are 0.2 mm/s, 13.3 m/s, and 13 measurements/sec, respectively. A graphic user interface is added to show the velocity and direction of the wind with the speed of sound and temperature of the wind in the 3 dimensional space. The new anemometer could be useful for the measurement of the air speed, the flow of fluids, and even air flow inside the downtown buildings.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2002년도 ITC-CSCC -3
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• pp.1455-1458
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• 2002

In this paper, we propose a modified CP-AFC(Cross-Product Automatic Frequency Control) algorithm to enhance coherent signal detection for WCDMA reverse link receiver. We introduce a moving average filter at the FDD(Frequency Difference Detector) input to increase the number of cross-products, since pilot symbol in WCDMA is not transmitted continuously. We also add normalization algorithm to overcome the conventional CP-FDD's sensitivity to the variance of input signal amplitude and to increase the linear range of S- curve. For rapid frequency acquisition and tracking, we adopt a multi-stage tracking mode. We applied the proposed algorithm in the implementation of WCDMA base station modem successfully.

• 산업경영시스템학회지
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• 제33권2호
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• pp.105-111
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• 2010

The purpose of this study was to detect early children with hyperactivity which is one of the symptoms of Attention Deficit-Hyperactivity Disorder (ADHD). This study used two methods: K-CBCL and observation of children's behavior. K-CBCL was done online by parents at home. For observation of children's behavior, the school asked children to wear a 3-axis accelerometer on their wrists. The data from K-CBCL and 3-axis accelerometer were analyzed and clustered to separate hypersensitive children from ordinary children. This experiment confirmed that 3-axis accelerometer which is one of Ubiquitous techniques and the K-CBCL questionnaire were helpful for detection of hypersensitive children.