• Journal of Microbiology
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• v.42 no.3
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• pp.216-222
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• 2004

In a previous paper, the ogdH gene that encodes 2-oxoglutarat dehydrogenase was isolated from Salmonella typhimurium. The catalytic N-terminal region in the enzyme was found to be very specific for the Salmonella species. Therefore, the aim of the present study was to detect S. typhimurium in food sources using primers designed for OGDH-l and OGDH-2 which were based on the salmonella-specific region of the ogdH gene. A simple polymerase chain reaction (PCR) detection method was developed to detect low numbers of S. typhimurium in a chicken meat microbial consortium. Using the ogdH-specific primers under stringent amplification conditions and for gene probe analysis, fewer than 100 colony-forming units (CFUs) were detectable when pure cultures were employed. When the PCR assay was run on S. typhimurium-contaminated meat contents, only the positive meat samples containing as few as 200 CFUs reacted to the assay. The method employed for sample processing is simple and it was determined to provide a sensitive means of detecting trace amounts of S. typhimurium-specific sequences in the presence of mixed meat microbial populations. When compared with six representative intestinal gram-negative bacterial strains in foods, including Vibrio parahaemolyticus, V. vulnificus, Enterobacter cloacae, E. coli O157:H7, Pseudomonas aeruginosa, and Proteus sp., S. typhimurium had a unique and distinct PCR product (796 bp). In conclusion, the two OGDH primers were found to be rapid and sensitive detectors of Salmonella spp for the PCR method.

• Korean Journal of Remote Sensing
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• v.32 no.6
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• pp.673-679
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• 2016

This study propose a new method to detect Cochlodinium polykrikoides red tide occurring in South Sea of Korea using Water-leaving Radiance data and Absorption Coefficients data of Geostationary Ocean Color Imager (GOCI). C. polykrikoides were analyzed and the irradiance and light emission characteristics of the wavelength range from 412 nm to 555 nm were confirmed. The detection technique proposed in this study detects the red tide occurring in the optically complex South Sea. Based on these results, it can be used for future red tide prevention.

• Journal of Information Technology Services
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• v.10 no.1
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• pp.105-116
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• 2011

With recent spotlight on the, uniquitous computing technology, the need for object of indentification and location infrastructure has increased. Such GPS technolgy must utilize IEEE 802.15.4 Zigbee used for existing wireless sensor network infra as a basice element for user's context-awareness in a uniquitous environement, for effectiveness.Such real-time GPS service is provided in the internal environment where the user would actually are and most high-rise buildlings apply. Underthe assumption, the real-time GPS technology is seperated by each floor, and signals do not get transmitted to other floors, the application on one floor within the high-rise buildling was conducted. This study intends to suggest a floor detection algorithm using IEE 802.15.3/Zigbee's RSSI which supports the accuracy within a couple of meters for the user's the movement between the floors in high-rise buildings in a complex environment. It proposes an floor detection algorithm using IEEE 802.15.4/Zigbee's RSSI which provides accuracy within a radius of few meters for the users movement between the floors for real-time location tracking within high-rise building in a cmoplex environment. Furthermore, for more accurate real-time location tracking, it suggests an algorithm for real-time location tracking using IEEE 802.15.4a/Zigbee's CSS technology based on triangulation. Based on the suggested algorithm, it designs a hybrid real-time location tracking service system in a high-rise buildling and test its functions.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.29 no.5
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• pp.1019-1026
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• 2019

Due to limitations of existing methods for detecting newly introduced malware, the importance of the development of artificial intelligence vaccines arises. Existing artificial intelligence vaccines have a disadvantage that the accuracy of the detection rate is low because those vaccines do not scan all parts of the file. In this paper, we suggest an enhanced method for detecting malware which is composed of unique OP Code features in the malware files. Specifically, we tested the method with text datasets trained on Random Forest algorithm and with image datasets trained on the Inception V3 model. As a result, the highest accuracy of the detection rate was about 80%.

• Journal of Bone Metabolism
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• v.25 no.4
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• pp.235-241
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• 2018

Background: Procollagen type I N-terminal propeptide (PINP) is one of the most clinically useful bone formation biomarkers. Therefore, the purpose of this study was to independently evaluate the performance of automated total PINP assay and established age- and gender- specific reference intervals for PINP in healthy Korean population. Methods: The imprecision, linearity, and detection capability of Elecsys total PINP assay was determined and reference interval was established using 599 serums from Korean population with normal bone mineral densities based on bone densitometry. Age groups were divided into 20s, 30s, 40s, 50s, 60s and over. Results: Elecsys total PINP had excellent performance in imprecision, linearity, and detection capability. When partitioning age groups in Korean male and female populations, there was significant difference in total PINP between different age groups. In male populations, PINP level was decreased with increasing age, then it remained steady after middle-age. In female populations, there was a decreasing tendency similar to that in the male population with a sharp increase in the 50 to 59 age group. Conclusions: Elecsys total PINP assay showed precise and reliable performance in our study. We established age-related PINP reference intervals for Korean male and female population with normal bone mineral densities.

• Korean Journal of Veterinary Service
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• v.34 no.4
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• pp.417-428
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• 2011

Species-specific PCR assay was developed for detection of cattle, sheep, goat, horse, dog, pig, chicken, duck, goose, and turkey using mitochondrial 12S rRNA and 16S rRNA as target genes. Also, an internal positive control was used to detect possible false negatives by using 18S rRNA gene. We designed species-specific primers with amplicon length of 190, 219, 350, 467, 241, 119, 171, 229, 111 and 268 bp for cattle, sheep, goat, horse, dog, pig, chicken, duck, goose, and turkey respectively. The specificity of the primers was tested against the other 10 non-target animal species and a cross-reaction was not observed. We developed two multiplex PCR assays for the simultaneous identification of Korea's major livestock species (cattle, pig, chicken and duck) and poultry species (chicken, duck, goose and turkey) from analogous samples, retaining the same specificity. The limit of detection of the multiplex PCR assay (cattle, pig, chicken and duck) ranged between 1 pg and 0.1 pg of template DNA extracts from raw meat. Applying multiplex PCR assays to DNA extracts from experimental pork/beef and pork/chicken tested raw and heat-treated ($120^{\circ}C$ for 30 min) mixtures respectively, detection limit was 0.1% level beef in pork, pork in beef and chicken in pork and 1.0% level pork in chicken. In conclusion, this assay using gel-based capillary electrophoresis would be very useful in highly sensitive and rapid identification of animal species or ingredients in minced meat and other meat products.

• Journal of Genetic Medicine
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• v.5 no.1
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• pp.7-14
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• 2008

Twenty years have passed since a prenatal screening for Down syndrome and neural tube defect was applied to obstetric field. The Quad test (AFP, hCG, uE3, Inhibin-A) of the second trimester and the combination test (PAPP-A, hCG, NT) of the first trimester became popular now. The recent trend of prenatal screening is to combine these two screening tests together in order to increase a detection rate of Down syndrome. Three types of screening methods are introduced as follows; integrated test, sequential test and contingent test. In addition to combination of each test, an incorporation of characteristic ultrasound findings of Down syndrome is suggested for its risk calculation. The absence of fetal nasal bone would be a very useful marker especially in the first trimester screening test. According to a change of way calculating risk of Down syndrome, obstetrician's role will be more increased not by passive participation, but by active participation using ultrasound in risk calculation.

• Journal of Sensor Science and Technology
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• v.15 no.6
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• pp.442-448
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• 2006

Ultrasonic anemometers using pulse envelope detection-based method are standard instruments in most meteorological studies. In this paper, a new phase measurement method is tried to achieve the enhanced resolution without changing dimensions. The measurement sensitivity, dynamic range, and measurement speed of the new instrument are 0.2 mm/s, 13.3 m/s, and 13 measurements/sec, respectively. A graphic user interface is added to show the velocity and direction of the wind with the speed of sound and temperature of the wind in the 3 dimensional space. The new anemometer could be useful for the measurement of the air speed, the flow of fluids, and even air flow inside the downtown buildings.

• Proceedings of the IEEK Conference
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• 2002.07c
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• pp.1455-1458
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• 2002

In this paper, we propose a modified CP-AFC(Cross-Product Automatic Frequency Control) algorithm to enhance coherent signal detection for WCDMA reverse link receiver. We introduce a moving average filter at the FDD(Frequency Difference Detector) input to increase the number of cross-products, since pilot symbol in WCDMA is not transmitted continuously. We also add normalization algorithm to overcome the conventional CP-FDD's sensitivity to the variance of input signal amplitude and to increase the linear range of S- curve. For rapid frequency acquisition and tracking, we adopt a multi-stage tracking mode. We applied the proposed algorithm in the implementation of WCDMA base station modem successfully.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.33 no.2
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• pp.105-111
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• 2010

The purpose of this study was to detect early children with hyperactivity which is one of the symptoms of Attention Deficit-Hyperactivity Disorder (ADHD). This study used two methods: K-CBCL and observation of children's behavior. K-CBCL was done online by parents at home. For observation of children's behavior, the school asked children to wear a 3-axis accelerometer on their wrists. The data from K-CBCL and 3-axis accelerometer were analyzed and clustered to separate hypersensitive children from ordinary children. This experiment confirmed that 3-axis accelerometer which is one of Ubiquitous techniques and the K-CBCL questionnaire were helpful for detection of hypersensitive children.