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A Role for buttonhead in the Early Head and Trunk Development in the Beetle Tribolium castaneum

  • Jeon, Haewon;O, Jiyun;Jin, Sil;Lim, Jinsung;Choe, Chong Pyo
    • Development and Reproduction
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    • v.23 no.1
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    • pp.63-72
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    • 2019
  • The head gap gene buttonhead (btd) is required for the patterning of head segments in the early Drosophila embryo. Mutant phenotypes of btd display a gap-like phenotype in which antennal, intercalary, mandibular and the anterior portion of the maxillary segments are eliminated. In agreement with the phenotypes, btd is expressed in a stripe covering the head segments at the blastoderm stage. During the early phase of the germband extension, btd is expressed in stripes with single segmental periodicity, which is required for the formation of the peripheral nervous system. In contrast to the key role of btd in Drosophila embryonic development, it has been suggested that Tribolium ortholog of btd (Tc-btd) is dispensable for embryonic head development. In order for better understanding of the requirement of Tc-btd in the early Tribolium embryo, we re-analyzed the expression patterns and functions of Tc-btd during embryonic segmentation. Tc-btd is expressed in segmental stripes at the stages of blastoderm and germband elongation. Up to 28.3% of embryos in which Tc-btd is knocked down displays the loss of antennal, mandibular and the pregnathal regions in the head, with abdominal segments being disrupted in the trunk. Our findings suggest that Tc-btd is required for the head and trunk development in the early Tribolium embryo.

Density Estimation of Japanese Common Squid Todarodes pacificus Using Multi-frequency (다중주파수를 이용한 살오징어(Todarodes pacificus)의 분포밀도 추정)

  • Shin, Hyoung-Ho;Jung, Jongil;Lee, Hyoungbeen;Oh, Wooseok;Park, Geunchang;Lee, Kyounghoon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.54 no.6
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    • pp.1023-1029
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    • 2021
  • The Japanese common squid Todarodes pacificus is one of the fish species within the total allowable catch (TAC) system which requires further investigation. In this study, the acoustic survey method was used to analyze the distribution of the Japanese common squid Todarodes pacificus across all the seas of South Korea. The sea area within Korea was investigated using the research vessels 20, 21, and 22 of the National Institute of Fisheries Science. The acoustic surveys were carried out from July to September 2019 and February to May 2020. The acoustic systems used in the survey had frequencies of 38 kHz and 120 kHz (EK60, EK80, Simrad, Norway) of the split-beam scientific echosounder. The results showed that, in spring, 277 m2/nmi2 was the highest in the east sea area, and the same in the summer season 880 m2/nmi2 was the highest in the east sea area. In autumn, the highest nautical area scattering coefficient (NASC) value was observed in the coastal portion of the south sea, and in winter, the NASC values were generally low in all the sea area.

Mineral Content and Nutritional Evaluation of Marine Invertebrates from the Korean Coast (한국 연안산 해산 무척추동물의 미네랄 함량과 영양평가)

  • Mok, Jong-Soo;Lee, Doo-Seog;Shim, Kil-Bo;Yoon, Ho-Dong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.42 no.2
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    • pp.93-103
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    • 2009
  • To measure the mineral contents of marine invertebrates, we collected 239 individuals from 52 species from the east (Pohang), west (Gunsan), and south (Tongyeong) coasts of Korea: 34 species of molluscan shellfish (Gastropoda and Bivalvia), 6 species of Cephalopoda, 8 species of Crustacea, and 4 other species. The mean contents of the macro mineral in the 100g edible portion of molluscan shellfish were high in the oder of Na (355.1 mg), P (128.1 mg), K (110.8 mg), Mg (83.8 mg) and Ca (57.5 mg). And the mean contents of the micro mineral in the molluscan shellfish were high in the oder of Fe ($41.12{\mu}g/g$), Zn ($30.48{\mu}g/g$), Mn ($3.81{\mu}g/g$), Cu ($3.49{\mu}g/g$) and Ni ($0.35{\mu}g/g$). We could not observe clear regional variation in the mineral content of the molluscan shellfish species. The mean contents of the macro mineral in the 100g edible portion of other marine invertebrates (Cephalopoda, Crustacea, etc.) were high in the oder of Na (400.7 mg), P (145.1 mg), K (105.0 mg), Mg (63.2 mg) and Ca (49.0 mg). And the mean contents of the micro mineral in other marine invertebrates were high in the oder of Zn ($24.4{\mu}g/g$), Fe ($13.8{\mu}g/g$), Cu ($4.88{\mu}g/g$), Mn ($2.14{\mu}g/g$) and Ni ($0.12{\mu}g/g$). The daily average intakes of the macro mineral through fisheries products consisted of P (125.9 mg), Na (119.3 mg), K (101.8 mg), Ca (31.0 mg), Mg (27.6 mg). And the daily average intakes of the micro mineral through fisheries products consisted of Zn (0.92 mg), Fe (0.68 mg), Cu (0.12 mg) and Mn (0.07 mg). The respective intakes of the minerals (P, Mg, Na, Ca, K) from fish were about 18.0, 7.9, 8.0, 4.4 and 2.2% of the dietary reference intakes for Koreans (KDRIs) set by the Korean Nutrition Society. And the respective intakes of micro mineral (Cu, Zn, Fe, Mn) from fish were about 14.6, 9.2, 6.7, and 2.1% of the dietary reference intakes for Koreans (KDRIs). Therefore, fisheries products play a very important role as a source of mineral.

Diversity of Calpain-Calpastatin gene frequencies in Brown, Brindle and Jeju Black Hanwoo (한우, 칡소 및 제주 흑우 Calpain-Calpastatin 유전자 다양성)

  • Lee, Seung-Hwan;Kim, Seung-Chang;Cho, Soo-Hyun;Choi, Bong-Hwan;Sharma, A.;Lim, Dajeong;Dang, Chang-Gwan;Chang, Sun-Sik;Kim, Jae-Hwan;Ko, Moon Suk;Yang, Boh-Suck;Kang, Hee-Sul
    • Korean Journal of Agricultural Science
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    • v.40 no.2
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    • pp.147-153
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    • 2013
  • The aim of study was to investigate genetic diversity for the calpain/calpastatin gene in three Hanwoo breeds [(Brown (n=62), Brindle (n=81) and Jeju Black (n=30)]. Random samples from three breeds of Hanwoo were selected and genotyped for the 7 SNPs of calpain/calpastatin using TaqMan method. Allele frequencies were investigated for CAPN1/CAST gene. Allele frequency of CAST2 SNP was 0.75, 0.59 and 0.22 for Brown, Brindle and Jeju black, respectively. The CAST3 revealed allele frequency of 0.59 and 0.57 in Brown and Jeju Black, while it showed very low allele frequency (0.07) in Brindle. In particular, favorable allele (G allele) for the CAPN1-2 SNP which was shown a strong association with tenderness in Taurine and Indicine cattle revealed 16% and 17% higher allele frequency in Brown Hanwoo (0.82) comparing Brindle (0.66) and Jeju Black Hanwoo (0.65). AMOVA demonstrated that among population variance occupied only 10% of total variance and among individual variance was 0%, while within individual variance was 90% of total variance. This result showed that population effect contributed very small portion of genetic to these three Hanwoo breeds, while within individual variance contributed large portion of genetic diversity within these Hanwoo breeds. In conclusion, three Hanwoo breeds (Brown, Brindle and Jeju black) showed a genetically homogeneous based on the 7 SNPs of CAPN1/CAST gene and it came from same ancestor to form modern Hanwoo breed.

Morphological Comparison Hysterothylacium sp. and Anisakis simplex (Nematoda: Anisakidae) from Wild Black Rockfish, Sebastes schlegeli, and Histopathological Host Reaction (자연산 조피볼락, Sebastes schlegeli의 소화관에 기생하는 Hysterothylacium sp.와 Anisakis simplex (Nematoda: Anisakidae)의 형태학적 비교 및 숙주의 조직병리학적 반응)

  • Park, Jung-Jun;Park, Myoung-Ae;Choi, Hye-Sung;Kim, Seok-Ryel
    • Applied Microscopy
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    • v.41 no.3
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    • pp.205-213
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    • 2011
  • Numerous anisakids were parasitic near the digestive tract of the black rockfish, Sebastes schlegeli and some anisakids observed on the liver of the host. Anisakids in the host were identified three species (Hysterothylacium sp., Anisakis simplex, A. pegreffii) and Hysterothylacium sp. was the high occurrence of anisakid worms in the host. Hysterothylacium sp. was shorter and thinner than A. simplex. Both of anisakids observed lip, mouth, nerve ring, excretory pore and excretory duct in the anterior portion. In the mid portion, anisakids had esophagus, ventriculus and intestine and especially, Hysterothylacium sp. had intestinal ceacum and ventricular appendage. There was conical nodulose apex at the end of the posterior portion in Hysterothylacium sp. and spine in A. simplex. SEM examination revealed that there was three lip near the mouth of Hysterothylacium sp. Dorsal lip was approximately 65 ${\mu}m$ and paried lateroventral lip were approximately 60 ${\mu}m$ in the width. All lips were found double papilla (approximately 8 ${\mu}m$ in the width). The body width of the Hysterothylacium sp. and A. simplex was approximately 480 ${\mu}m$ and 900 ${\mu}m$ respectively. The hight of the lateral alae was about 7 ${\mu}m$ and width of papilla on the cornical nodulose apex was about 3.3 ${\mu}m$ in Hysterothylacium sp. The hight of spine was approximately 20 ${\mu}m$ in A. simplex. There was mainly Hysterothylacium sp. in the intestinal lumen of the host. The nematod worms were parasitic near the mucosal fold and in the submucosal. In the mucosal epidermal layer, it was increased mucous cells by the infection of the parasites.

Histopathological and DNA Content Analysis of a Dermal Sarcoma in the Soft-shelled Turtle Pelodiscus sinensis

  • Syasina Iraida Germogenovna;Hur Jun-Wook;Kim Eun-Mi;Park In-Seok
    • Fisheries and Aquatic Sciences
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    • v.9 no.3
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    • pp.107-114
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    • 2006
  • A dermal sarcoma was found in a freshwater, soft-shelled turtle Pelodiscus sinensis. The neoplasm consisted of proliferating fibrous tissue and extended from the dermis. The overlying epidermis was hyperplastic and partially folded. The deeper dermis and hypodermis contained three large, discrete necrotic foci of -10 mm diameter. Numerous eosinophilic granule cells and macro phages surrounded the necrotic areas. A mixed population of cells with nuclear pleomorphism was observed between the papillary layers of vessels. This area also had regions of different histological structures: (l) regularly arranged, spindle-shaped cells with compact nuclei in a fine-fibrillar matrix; (2) haphazardly arranged cells ($\leq$ 23 11m diameter) with ovoid, highly hypertrophic, faintly stained nuclei; and (3) cells (3.6-5.8 11m diameter) with irregularly shaped nuclei and marginal condensed chromatin in a myxomatous matrix. Some mitotic figures, binucleate cells, and multinucleate giant cells of up to 50 11m in length were also found. Flow cytometry of propidium iodide-stained cells yielded different histograms for the normal skin and the skin (primarily epidermis) and fibrous dermis of the tumor, indicating DNA heterogeneity in the dermal portion of the tumor. The ploidy indices for the dermal cells were 1.91 and 0.78, as compared to normal cells.

MicroRNA Target Recognition: Insights from Transcriptome-Wide Non-Canonical Interactions

  • Seok, Heeyoung;Ham, Juyoung;Jang, Eun-Sook;Chi, Sung Wook
    • Molecules and Cells
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    • v.39 no.5
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    • pp.375-381
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    • 2016
  • MicroRNAs (miRNAs) are small non-coding RNAs (~22 nucleotides) regulating gene expression at the post-transcriptional level. By directing the RNA-induced silencing complex (RISC) to bind specific target mRNAs, miRNA can repress target genes and affect various biological phenotypes. Functional miRNA target recognition is known to majorly attribute specificity to consecutive pairing with seed region (position 2-8) of miRNA. Recent advances in a transcriptome-wide method of mapping miRNA binding sites (Ago HITS-CLIP) elucidated that a large portion of miRNA-target interactions in vivo are mediated not only through the canonical "seed sites" but also via non-canonical sites (~15-80%), setting the stage to expand and determine their properties. Here we focus on recent findings from transcriptome-wide non-canonical miRNA-target interactions, specifically regarding "nucleation bulges" and "seed-like motifs". We also discuss insights from Ago HITS-CLIP data alongside structural and biochemical studies, which highlight putative mechanisms of miRNA target recognition, and the biological significance of these non-canonical sites mediating marginal repression.

Terabit-Per-Second Optical Super-Channel Receiver Models for Partial Demultiplexing of an OFDM Spectrum

  • Reza, Ahmed Galib;Rhee, June-Koo Kevin
    • Journal of the Optical Society of Korea
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    • v.19 no.4
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    • pp.334-339
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    • 2015
  • Terabit-per-second (Tb/s) transmission capacity for the next generation of long-haul communication networks can be achieved using multicarrier optical super-channel technology. In an elastic orthogonal frequency division multiplexing (OFDM) super-channel transmission system, demultiplexing a portion of an entire spectrum in the form of a subband with minimum power is critically required. A major obstacle to achieving this goal is the analog-to-digital converter (ADC), which is power-hungry and extremely expensive. Without a proper ADC that can work with low power, it is unrealistic to design a 100G coherent receiver suitable for a commercially deployable optical network. Discrete Fourier transform (DFT) is often seen as a primary technique for understanding partial demultiplexing, which can be attained either optically or electronically. If fairly comparable performance can be achieved with an all-optical DFT circuit, then a solution independent of data rate and modulation format can be obtained. In this paper, we investigate two distinct OFDM super-channel receiver models, based on electronic and all-optical DFT-technologies, for partial carrier demultiplexing in a multi-Tb/s transmission system. The performance comparison of the receivers is discussed in terms of bit-error-rate (BER) performance.

Mechanical Properties and Ultrasonic Characteristic of SS400 and STS304 by Simulated Heats (열재현에 의한 SS300 및 STS304의 기계적 성질 및 초음파 특성)

  • Jeong, Jeong-Hwan;Ahn, Seok-Hwan;Park, In-Duck;Nam, Ki-Woo
    • Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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    • 2003.10a
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    • pp.127-132
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    • 2003
  • In a today industry, the welding is doing a many portion in structure manufacture. This study is simulated heat of heat-effected zone and researched a mechanical properties and ultrasonic characteristic in used the SS400 and the STS304. As the result mechanical properties of steel that become drawing decreased because of remaining stress by strain gardening according as simulated heat temperature rises, but according as temperature rises in material that do simulated heat after have done annealing, mechanical propensity was improved. The velocity and attenuation become different by effect of remaining stress than effect of material internal microstructure in ultrasonic wave test. In the case of STS304, there was change in mechanical properties by effect that is by strain hardening, but there was no change in material that simulated heat after annealing. When become drawing in ultrasonic waves test, according as simulated heat temperatures rise, change of attenuation coefficient is looked, but material that simulated heat after annealing was no change almost both the volocity and attenuation.

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A Study on the Heat Transfer Characteristics of a Self-Oscillating Heat Pipe

  • Yoon, Seok-Hun;Cheol Oh;Park, Jae-Hyuk
    • Journal of Mechanical Science and Technology
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    • v.16 no.3
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    • pp.354-362
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    • 2002
  • In this paper, the heat transfer characteristics of a self-oscillating heat pipe are experimentally investigated for the effect of various working fluid fill charge ratios and heat loads. The characteristics of temperature oscillations of the working fluid are also analysed based on chaotic dynamics. The heat pipe is composed of a heating section, a cooling section and an adiabatic section, and has a 0.002m internal diameter, a 0.34m length in each turn and consists of 19 turns. The heating and the cooling portion of each turn has a length of 70mm. A series of experiments was carried out to measure the temperature distributions and the pressure variations of the heat pipe. Furthermore, heat transfer performance, effective thermal conductivity, boiling heat transfer and condensation heat transfer coefficients are calculated for various operating conditions. Experimental results show the efficacy of this type of heat pipe.