• KSBB Journal
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• v.18 no.2
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• pp.155-160
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• 2003

The effect of dissolved oxygen(DO) on microbial transglutaminase(mTG) production by Streptoverticillium morbaraense was studied in on-line computer controlled fermentation system. In order to control dissolved oxygen during fermentation, the agitation speed and aeration rate of 2.5 L fermenter ranged from 260 to 360 rpm and 0.3 to 3.9 L/min, respectively. The maximum microbial transglutaminase production was obtained at controlled 20% of dissolved oxygen among the various dissolved oxygen controlled batch cultures tested. The production of microbial transglutaminase at controlled 20% of dissolved oxygen was about 2.12 U/mL which was 1.1 times higher than that obtained in batch culture without control of dissolved oxygen. Also, the highest microbial transglutaminase production was obtained in fed-batch cultures in which dissolved oxygen was controlled at 20%, and it was improved almost 1.3 times in comparison with that without control of dissolved oxygen. Maximal dry cell weight and microbial transglutaminase production were 13.2 g/L and 2.6 U/mL, respectively. Finally, it was also found that fed-batch fermentation at controlled 20% of dissolved oxygen showed a good performance for the microbial transglutaminase production by on-line computer controlled fermentation system which may be generally applicable to other microbial cultures.

• Journal of Advanced Marine Engineering and Technology
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• v.26 no.3
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• pp.375-381
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• 2002

Measuring of the dissolved oxygen is widely used for the environmental control of natural waters, sewage waste treatment, medical and biochemical studies, soil husbandry, food and drug process control, and prevention of corrosion in boilers. Especially, a power plants need special management for preventing accidents from corrosion, therefore, it is essential to measure the concentration of dissolved oxygen in real-time. In this paper we present a method of measuring dissolved oxygen very accurately up to pub units. This method, called polarograph method, is based on the measures of the electric current generated by the oxidation process in cathode and de-oxidation process in anode, assuming that the amount of the current is proportional to the density of dissolved oxygen.

• Proceedings of the Korean Society of Marine Engineers Conference
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• 2001.05a
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• pp.197-201
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• 2001

Measuring of the dissolved oxygen is widely used for the environmental control of natural waters, sewage waste treatment, medical and biochemical studies, soil husbandry, food and drug process control, and prevention of corrosion in boilers. Especially, a power plants need special management for preventing accidents from corrosion, therefore, it is essential to measure the concentration of dissolved oxygen in real-time. In this paper we present a method of measuring dissolved oxygen very accurately up to ppb units. This method, called polarographic method, is based on the measures of the electric current generated by the oxidation process in cathode and de-oxidation process in anode, assuming that the amount of the current is proportional to the density of dissolved oxygen.

• Journal of Advanced Marine Engineering and Technology
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• v.35 no.5
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• pp.640-646
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• 2011

Dissolved oxygen (DO) refers to the volume of oxygen that is contained in water, and is a major indicator of water quality. The objective of this paper was to investigate the effect of salinity on the dissolved oxygen characteristics in an ejector-aerator. An experimental aeration system composed of a motor-pump, an ejector, a motor-blower, a set of aeration and recirculation tank and a control panel. The dissolved oxygen concentrations decreased with the water salinity. The volumetric mass transfer coefficient increased with increasing the water salinity.

• Biotechnology and Bioprocess Engineering:BBE
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• v.3 no.2
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• pp.109-111
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• 1998

The effect of dissolved oxygen concentration on the metabolism of glucose in Pseudomonas putida BM014 was investigated. Glucose was completely converted to 2-ketogluconate via extracellular oxidative pathway and then taken up for cell growth under the condition of sufficient dissolved oxygen concentration. On the other hand, oxygen limitation below dissolved oxygen tension (DOT) value of 20% of air saturation caused the shift of glucose metabolism from the extracellular oxidative pathway to the intracellular phosphorylative pathway. Specific activities of hexokinase and gluconate kinase in intracellular phosphorylation pathway decreased as the DOT increased, while 2-ketogluconokinase activity in extracellular oxidative pathway increased under the same condition. This result can be usefully applied to microbial transformation of glucose to 2-ketogluconate, the synthetic precursor for iso-vitamine C, with almost 100% yield via extracellular oxidation by simple DOT control.

• Nuclear Engineering and Technology
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• v.31 no.6
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• pp.541-547
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• 1999

Corrosion control, in the end-shield cooling system of Wolsung Nuclear Power Plant, is directly related to the control of dissolved oxygen (DO). The current method, being used to deoxygenate the end-shield cooling water, is a chemical treatment by addition of reducing agent, hydrazine, to react with DO. This method has several limitations including high reaction temperature of hydrazine , unwanted explosive hydrogen gas production, and its intrinsic harmful property. A new approach to remove DO using a membrane-based oxygen removal system (MORS) was tried to overcome limitations of the hydrazine treatment. The DO removal efficiency of the MORS was found to be in the range 87% to 98%: The higher vacuum, the lower water flow rate and the higher water temperature tend to increase the DO removal efficiency.

• KSBB Journal
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• v.15 no.3
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• pp.280-284
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• 2000

The simple imcroprocessor device was provided for the control of dissolved oxygen(DO) level. The DO analog signal output from fementer was used as a measurement variable and the agitation speed of fermenter was used as a manipulatio variable. Agitation speed was manipulated to maintain DO level above 10% of saturation condition during cell growth period with microprocessor. Since the experimental resultsshowed that the DO level was maintained above 10% and agitation speed was inverse proportional to DO level ithis simple control device can be used for the DO control of fermenter.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.59 no.4
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• pp.387-398
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• 2023

Conventional aquaculture faces declining productivity, shifting to recirculating aquaculture system (RAS), known for minimizing water usage and maintaining consistent water temperatures for year-round fish growth. Rainbow trout (Oncorhynchus mykiss), a globally important cold-water species and the third most farmed fish in inland waters of Korea, valued for its fecundity and rapid growth. Dissolved oxygen, an important environmental factor affecting fish production and economics, highlights the need for smart aquaculture practices. Since 2018, the rise of intelligent aquaculture platforms, incorporating information and communications technology (ICT), emphasizes the essential role of RAS implementation. This eight-week study aimed to determine the optimal dissolved oxygen concentration for rainbow trout in RAS, utilizing a device for continuous monitoring, control and record. Dissolved oxygen concentrations were set at 5-6 mg/L, 9-10 mg/L, 14-15 mg/L and 17-18 mg/L. The growth rate significantly decreased at 5-6 mg/L, with no significant differences in other experimental groups. In hematological analysis, growth hormone (GH) was significantly highest at 5-6 mg/L, followed by 9-10 mg/L while Insulin-like growth factor-1 (IGF-1) was significantly lowest at 5-6 mg/L. In conclusion, the optimal dissolved oxygen concentration for rainbow trout in RAS is approximately 9-10 mg/L. Higher concentrations do not contribute to further growth or profitability.

• Microbiology and Biotechnology Letters
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• v.21 no.4
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• pp.354-365
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• 1993

A novel control method with automatic tuning of PID controller parameters has been developed for efficient regulation of dissolved oxygen concentration in fed-batch fermentations of Escherichia coli. Agitation speed and oxygen partial pressure in the inlet gas stream were chosen to be the manipulated variables. A heuristic reasoning allowed improved tuning decisions from the supervision of control performance indices and it coule obviate the needs for process assumptions or disturbance patterns. The control input consisted of feedback and feedforword parts. The feedback part was determined by PID control and the feedforward part is determined from the feed rate. The proportional gain was updated on-line by a set of heuristics rules based on the supervision of three performance indices. These indices were output error covariance, the average value of output error, and input covariance, which were calculated on-line using a moving window. The integral and derivative time constants were determined from the period of output response. The specific growth rate was maintained at a low level to avoid acetic acid accumulation and thus to achieve a high cell density. The specific growthe rate was estimated from the carbon dioxide evolution rate. In fed-batch fermentation, the simutaneous control of dissolved oxygen concentration (at 0.2; fraction of saturated value) and specific growth rate (at 0.25$hr^{-1}$) was satisfactory for the entire culture period in spite of the changes in the feed rate and the switching of control input.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.240-244
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• 2003

A novel integrated method for increasing dissolved oxygen concentration in culture media has been developed. It involves adding hydrogen peroxide to the medium, which is then decomposed to oxygen and water by catalase and adding vegetable oil to the medium as antifoam agent and oxygen vector. A new apparatus for automated addition of hydrogen peroxide to the bioreactor to keep the dissolved oxygen concentration constant over the range $10-100%\;{\pm}\;5%$ was tested. A significant increase (over threefold) of cultivation time was obtained while the dissolved oxygen concentration remained stable ($30%\;{\pm}\;5%$). Therefore, use of corn oil mixed with Ca-stearate as oxygen vector and antifoam and hydrogen peroxide as oxygen source to control excessive foam that was generated by microorganism biosurfactant, GB16-BS produced at Bacillus sp. GB16 cultivation was appropriate for stable cultivation.