• Journal of Life Science
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• v.25 no.8
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• pp.903-909
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• 2015

Loop-mediated isothermal amplification (LAMP), a PCR-based diagnostic method, is based on autocycling strand displacement DNA synthesis in the presence of exonuclease-negative Bst DNA polymerase under isothermal conditions. With the help of four specific primers that recognize six different sequences of a target DNA, LAMP has high specificity in pathogenic identification in a short time. Hence, in the present study, LAMP is used as a diagnostic tool in the identification of the most dreadful aquatic pathogenic species, Vibrio alginolyticus, and to develop species-specific LAMP primers and optimization of LAMP reaction conditions such as annealing temperature, elongation time, and other PCR chemical concentrations, including MgSO₄, dNTPs, Betaine, and Bst polymerase. The optimized LAMP primers were also checked for specificity with other Vibrio species, which showed that the designed primers were very specific to V. alginolyticus After the first introduction of a species name like this one, the first part (“Vibrio” in this case) should be abbreviated to only the first letter.only. These are usually the most harmful pathogens of the Vibrio species that appear in shrimp and crabs. The results also revealed that the LAMP assay could be 10-fold more sensitive than conventional PCR in detecting V. alginolyticus. This could be the first report on using a rapid and highly sensitive technique, the LAMP assay, in the effective diagnosis of the pathogenic bacteria V. alginolyticus, which could help in the early detection of diseases, particularly in aquaculture.

• Composites Research
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• v.30 no.2
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• pp.84-93
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• 2017

This study investigates thermal and mechanical characterization of environmental barrier coating on the $SiC_f-SiC$ composites. The spherical environmental barrier coating (EBC) powders are prepared using a spray drying process for flowing easily during coating process. The powders consisting of mullite and 12 wt% of Ytterbium silicate are air plasma sprayed on the Si bondcoat on the LSI SiC fiber reinforced SiC composite substrate for protecting the composites from oxidation and water vapor reaction. We vary the process parameter of spray distance during air plasma spray of powders, 100, 120 and 140 mm. After that, we performed the thermal durability tests by thermal annealing test at $1100^{\circ}C$ for 100hr and thermal shock test from $1200^{\circ}C$ for 3000 cycles. As a result, the interface delamination of EBC never occur during thermal durability tests while stable cracks are prominent on the coating layer. The crack density and crack length depend on the spray distance during coating. The post indentation test indicates thermal tests influence on the indentation load-displacement mechanical behavior.

• The korean journal of orthodontics
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• v.28 no.1 s.66
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• pp.61-74
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• 1998

The Purpose of this study was to investigate the stress distribution and tooth displacement at the initial phase produced by 5 types of molar uprighting springs using finite element method. The three dimensional finite element model of lower dentition, bone and springs was composed of 5083 elements and 2071 nodes. The results were as follows: 1. In case of helical spring and root spring, intrusion of lower canine and first premolar were observed md distal tipping, translation and extrusion of lower second molar were observed. 2. In case of T-loop, modified T-loop and box loop, intrusion and distal translation of lower second premolar were observed, and the largest crown distal tipping and translation of lower second molar were observed in T-loop and the smallest were observed in box loop. 3. In case of T-loop with cinch-bact crown distal tipping and translation of lower second molar were decreased, but extrusion was also decreased. 4. With increase of activation in T-loop, mesial translation and won distal tipping of lower second molar were increased and edentulous space was closing, but distal translation of second premolar was also increased. 5. With increase of tip-back bend in T--loop, distal tipping and translation of lower second molar were increased, but extrusion was also increased more largely.

• Journal of the Korean Geotechnical Society
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• v.35 no.11
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• pp.25-36
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• 2019

The safety barrier is installed on road embankment to prevent vehicles from falling into road side slope. Among the safety barrier, flexible guardrails are usually installed. The flexible guardrail generally consists of a protection cross-beam and supporting in-line piles. These guardrail piles are installed nearby slope edge of road embankment because the side area of the road is much narrow. The protection cross-beam absorbs impact energy caused by vehicle collision. The pile-soil interaction also absorbs the rest of the impact energy and then, finally, the flexible guardrail system resists the impact load. This paper aims to investigate the pile-soil interaction subjected to impact load using centrifuge model tests. In this study, a single pile was installed in compacted residual soil and loaded under lateral impact load. An impact loading system was designed and developed available on centrifuge tests. Using this loading system, a parametric study was performed and the parameters include types of loading and ground. Finally, the ultimate bearing capacity of supporting pile under impact load was analyzed using load-displacement curve and soil reaction pressure distributions at ultimate were evaluated and compared with previous studies.

• Clinical and Experimental Reproductive Medicine
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• v.21 no.1
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• pp.1-11
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• 1994

The occurrence and time course of capacitation, acrosomal loss, and hyperactivated motility require quantitative definition in order to characterize fertile human sperm. Recently the method has been developed to estimate the quality of spermatozoa by using kinematic parameters such as curvilinear velocity(VCL), average path velocity(VAP), linearity(LIN), straightness(STR), amplitude of lateral head displacement(ALH), and beat cross frequence(BCF) from Computer Assisted Sperm Analysis (CASA). In this study, using the Hamilton Thorn motility analyzer HTM 2030(Hamilton Thorn Research, Beverly, MA), we attempted to identify the spermatozoa with hyperactivated motility (HA) objectively and to monitor hyperactivation of human spermatozoa during incubation in capacitating media and after treatment of calcium ionophore as compared with acrosome status. And we examined whether HA are related to the result of SPA. Semen samples obtained from 16 healthy men were prepared by swim up technique and preincubated in a capacitating media(modified BWW medium) for 5 hours and treated with calcium ionophore solution. The acrosome reaction was detected with PSA-FITC labelling of the acrosome and in vitro sperm ferilizing capacity was assessed by the zona free hamster ovum penetration assay (SPA). The incidence of hyperactivated sperm was 2.6% in fresh semen, 14.3% of the swim up population, 13.7% after 5h of incubation. Significant increase of percentage of hyperactivated sperm was observed after the incubation (p<0.05) but after treatment, no significant changes of percentage of hyperactivated sperm(l1.8%) in contrast to significant rise in the percentage of acrosome reacted cells. Correlation analysis failed to show any significant relationship between the percentage of sperm with HA and SPA score. In conclusion, although no direct correlations were found between the results of SPA and HA, hyperactivation of sperm is associated with capacitation and monitoring hyperactivated sperm will be expected as a method of evaluating the functional quality of sperm such as SPA.

• Bulletin of the Korean Chemical Society
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• v.34 no.12
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• pp.3795-3799
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• 2013

The second-order rate constants ($k_N$) have been measured spectrophotometrically for nucleophilic substitution reactions of Y-substituted-phenyl 2-methylbenzoates (6a-e) with a series of cyclic secondary amines in MeCN at $25.0{\pm}0.1^{\circ}C$. Comparison of the $k_N$ values for the reactions of 4-nitrophenyl 2-methylbenzoate (6d) with those reported previously for the corresponding reactions of 4-nitrophenyl 2-methoxybenzoate (5) reveals that 6d is significantly less reactive than 5, indicating that modification of 2-MeO in the benzoyl moiety of 5 by 2-Me (i.e., $5{\rightarrow}6d$) causes a significant decrease in reactivity. This supports our previous report that aminolysis of 5 proceeds through a six-membered cyclic intermediate, which is highly stabilized through intramolecular H-bonding interactions. The Br${\o}$nsted-type plot for the reactions of 6d with a series of cyclic secondary amines is linear with ${\beta}_{nuc}=0.71$, which appears to be a lower limit of ${\beta}_{nuc}$ for a stepwise mechanism with breakdown of an intermediate ($T^{\pm}$) being rate-determining step (RDS). The Br${\o}$nsted-type plot for the reactions of 6a-e with piperidine is curved, i.e., the slope of Br${\o}$nsted-type plot (${\beta}_{lg}$) decreases from -1.05 to -0.41 as the leaving-group basicity decreases. The nonlinear Br${\o}$nsted-type plot has been taken as evidence for a stepwise mechanism with a change in RDS (e.g., from the $k_2$ step to the $k_1$ process as the leaving-group basicity decreases). Dissection of $k_N$ into the microscopic rate constants associated with the reactions of 6a-e with piperidine (e.g., $k_1$ and $k_2/k_{-1}$ ratio) also supports the proposed mechanism.

• Journal of the Korean Geotechnical Society
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• v.30 no.4
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• pp.5-19
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• 2014

In this study, the structural analysis is performed on the method of shallow block and deep cement mixing pile, and then their characteristics and associated behaviors were analyzed. In the case of continuous beam analysis, the predicted settlement was very small, and shear force and bending stress are somewhat overestimated. The frame method is similar to numerical analysis in the internal force shallow block and long pile, but because the settlement of pile is underestimated, the additional calculation using the reaction of the long pile is necessary. For soil arching method and piled raft foundation method, the excessive axial force of long pile was predicted because the load sharing of pile is very large compared to the other methods. In the behavior of the shallow block and deep pile method, the settlement of shallow block and contact pressure are much in the center than the edge. In the estimating method considering the interaction between improved material and ground, the load sharing of the soil-cement pile ranges from 20% to 45%, and the stress ratio is 2.0~5.0 less than piled DCM. The maximum member forces at the boundary conditions of pile head are similar, but in fixed head the axial force and vertical displacement are different in accordance with pile arrangement.

• Journal of the Korean Geosynthetics Society
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• v.14 no.1
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• pp.1-10
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• 2015

Recently, several researches on the development of new economical anchor systems have been performed to support floating structures. This study focused on the group suction piles, which connect mid-sized suction piles instead of a single suction pile with large-diameter. The group suction pile shows the complex bearing behavior with translation and rotation, so it is difficult to apply conventional design methods. Therefore, the numerical modeling technique was developed to evaluate the horizontal bearing capacity of the group suction piles in clay. The technique models suction piles as beam elements and soil reaction as non-linear springs. To analyze the applicability of the modeling, the horizontal load-movement curves of the proposed modeling were compared with those of three-dimensional finite element analyses. The comparison showed that the modeling underestimates the capacity and overestimate the displacement corresponding to the maximum capacity. Therefore, the correction factors for the horizontal soil resistance was proposed to match the bearing capacity from the three-dimensional finite element analyses.

• Journal of the Korea Concrete Institute
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• v.25 no.3
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• pp.261-269
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• 2013

In this study, it was developed eco-friendly inorganic binding material concrete using ground granulated blast furnace slag and alkali activator (water glass, sodium hydroxides). Eight reinforced concrete beam using inoganic binding material concrete were constructed and tested under monotonic loading. The major variables were mixture ratio of alkali activator, type of admixture and admixture. Experimental programs were carried out to improve and evaluate the flexural performance of such test specimens, such as the load-displacement, the failure mode, the maximum load carrying capacity, and ductility capacity. All the specimens were modeled in scale-down size. The eco-friendly concrete using inorganic binding material encouraged alkali activation reaction was rapidly hardening speed and showed possibility as a high strength concrete. Also, the RC beams using new materials showed similar behavior and failed similarly with RC beam used portland cement. It is thought that eco-friendly inorganic binding material concrete can be used with construction material and product as a basic research to replace cement concrete. If there is application to structures in PC member as well as production of 2nd concrete product, it could be improved the productivity and reduction of construction duration etc.

• Composites Research
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• v.27 no.6
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• pp.248-253
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• 2014

In this study, tapered double cantilever beam specimens are designed with the variable of angle to investigate the fracture property at the bonded surface of adjoint structure. These specimens are made with four kinds of models as the length of 200 mm and the slanted angles of bonded surfaces on specimens of $6^{\circ}$, $8^{\circ}$, $10^{\circ}$ and $12^{\circ}$. By investigating experiment and analysis result of these specimens, the maximum loads are happened at 120 N, 137 N, 154 N and 171 N respectively in cases of the specimens with slanted angles of $6^{\circ}$, $8^{\circ}$, $10^{\circ}$ and $12^{\circ}$. As the analysis result approach the experimental value, it is confirmed to have no much difference with the values of experiment and analysis. It is thought that the material property can be investigated effectively on shear behavior of the material composed of aluminum foam bonded with adhesive through simulation instead of experiment by applying this study method.