• Title/Summary/Keyword: Dispensing method

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Automated Protein-Expression Profiling System using Crude Protein Direct Blotting Method

  • Kobayashi, Hironori;Torikoshi, Yasuhiro;Kawasaki, Yuko;Ishihara, Hideki;Mizumoto, Hiroshi
    • 제어로봇시스템학회:학술대회논문집
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    • 2003.10a
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    • pp.2356-2361
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    • 2003
  • Proteome research in the medical field is expected to accelerate the understanding of disease mechanism, and to create new diagnostic concept. For protein profiling, this paper proposes a new methodology named CPDIB (Crude Protein Direct Blotting). In the CPDIB procedure, crude protein sample is directly immobilized on a membrane and the expression of protein molecules in the sample are analyzed quantitatively by using a special device called ImmobiChip, where the membrane is used as a field of the immune reaction. The over-all structure of the ImmobiChip is based on the conventional Slot blot device. Mechanical improvement in the air-tightness of the case holding the membrane realizes the direct blotting and results in high performance of stability in the immune reaction. In the measurement of multiple proteins, a dispensing robot is used for increasing the efficiency of handling of liquid. Cooperation of the dispensing robot with the ImmobiChip for immobilizing proteins realizes automated and stable performance of the CPDIB procedure. This paper shows the evaluation of the air-tightness of the ImmobiChip, the ability of analyzing proteins using the CPDIB procedure and the performance of the automated equipment.

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Fabrication of Co-Planar Type Single Chamber SOFC with Patterned Electrodes (패턴된 전극을 가진 표면 전도형 단실형 고체산화물 연료전지의 제조)

  • Ahn, Sung-Jin;Kim, Yong-Bum;Moon, Joo-Ho;Lee, Jong-Ho;Kim, Joo-Sun
    • Journal of the Korean Ceramic Society
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    • v.43 no.12 s.295
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    • pp.798-804
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    • 2006
  • Co-planar type single chamber solid oxide fuel cell with patterned electrode on a surface of electrolyte has been fabricated by robo-dispensing method and microfluidic lithography. The cells were composed of NiO-GDC-Pd or NiO-SDC cermet anode, $(La_{0.7}Sr_{0.3})_{0.95}MnO_3$ cathode, and yttria stablized zirconia electrolyte. The cell performance at $900^{\circ}C$ was investigated as a function of electrode geometries, such as anode-to-cathode distance, numbers of electrode pairs. Relationship between OCV and I-V characteristics at the optimized operation condition was also studied by DC source meter under the mixed gas condition of methane, air, and nitrogen. An increase of anode-facing-cathode area leads to lower OCV due to intermixing between product gases of anode and cathode, which in turn decreases the oxygen partial pressure difference.

Hermetic Characteristics of Negative PR (Negative PR의 기밀 특성)

  • Choi, Eui-Jung;Sun, Yong-Bin
    • Journal of the Semiconductor & Display Technology
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    • v.5 no.2 s.15
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    • pp.33-36
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    • 2006
  • Many issues arose to use the Pb-free solder as adhesive materials in MEMS ICs and packaging. Then this study for easy and simple sealing method using adhesive materials was carried out to maintain hermetic characteristic in MEMS Package. In this study, Hermetic characteristic using negative PR (XP SU-8 3050 NO-2) as adhesive at the interface of Si test coupon/glass substrate and Si test coupon/LTCC substrate was examined. For experiment, the dispenser pressure was 4 MPa and the $200\;{\mu}m{\Phi}$ syringe nozzle was used. 3.0 mm/sec as speed of dispensing and 0.13 mm as the gap between Si test coupon and nozzle was selected to machine condition. 1 min at $65^{\circ}C$ and 15 min at $95^{\circ}C$ as Soft bake, $200\;mj/cm^2$ expose in 365 nm wavelength as UV expose, 1 min at $65^{\circ}C$ and 6 min at $95^{\circ}C$ as Post expose bake, 60 min at $150^{\circ}C$ as hard bake were selected to activation condition of negative PR. Hermetic sealing was achieved at the Si test coupon/ glass substrate and Si test coupon/LTCC substrate. The leak rate of Si test coupon/glass substrate was $5.9{\times}10^{-8}mbar-l/sec$, and there was no effect by adhesive method. The leak rate of Si test coupon/LTCC substrate was $4.9{\times}10^{-8}mbar-l/sec$, and there was no effect by dispensing cycle. Better leak rate value could be achieved to use modified substrate which prevent PR flow, to increase UV expose energy and to use system that controls gap automatically with vision.

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Algorithm for Threat Data Integration of Multiple Sensor and selection of CounterMeasures (이기종 다중센서 위협데이터 통합 및 대응책 선정 알고리즘)

  • Go, Eun-Kyoung;Woo, Sang-Min;Jeong, Un-Seob
    • Journal of the Korea Institute of Military Science and Technology
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    • v.14 no.3
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    • pp.474-481
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    • 2011
  • The Electronic Warfare Computer for the Aircraft Survivability Equipment will improve the ability for countermeasures by analysis about threat information. This paper suggests method that threat data integration of multiple sensors(Radar Warning Receiver, Laser Warning Receiver, Missile Warning Receiver). The algorithm of threat data integration is based on detected threat sequence and azimuth information. The threat sequence information is analyzed in advance and the azimuth data is received from sensors. The suggested method is evaluated through simulation under the environment like real helicopter.

The Effect of the Optical Points Difference between Finished-Reading Glasses and Dispensing Reading Glasses (완성품 돋보기와 조제가공된 돋보기가 광학적 요소에 미치는 영향)

  • Shim, Young-Cheol;Yoo, Gun-Chang;Kim, In-Suk
    • Journal of Korean Ophthalmic Optics Society
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    • v.13 no.3
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    • pp.65-71
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    • 2008
  • Purpose: This paper studied the effect of eyes on the comparison between the distance optical centers problem of dispensing reading glasses made by optician and finished reading glasses in the current market. Methods: The method of this study has been measured by eleven different categories from +1.00D to +4.00D. This study also separated into three groups by their optical frame size and measured optical center point (O.C) and optical center height (O.H) with 200 peoples of man and females over 40 years old without ocular disease living in Gwang-san gu, Gwang-Ju city. Results: As a result, optical center point ranged from 57 mm to 80 mm and it turned to be most common range is from 61 mm to 65 mm (64.6%). Moreover, the optical center height ranged from 1 mm to 8mm and most common ranged (23%) were 4 mm. In other words, finished reading glasses have irregular optical ranges. After observing 200 people who are over 40 years old men and women, result shows that more than 75.5% (151 people) currently use finished reading glasses. Survey of 151 people, most common error between the finished reading glasses's O.C and the wearers P.D were 4 mm (45%). Furthermore, the most common error between the finished reading glassses's O.H and the wearers O.H ranged from 3 mm to 4 mm. Astonishingly, the entire 151 people who wear finished reading glasses appeal that they feel tiredness on their eyes when they wear finished reading glasses. 53 people (35%) claimed that they feel tiredness on their eyes after 10 to 20 minutes wearing finished reading glasses. Base on the research, We conducted more experiment to find the value of prism of optical centers err because it will tell us whether the finished reading glasses are good enough to wear or not. We multiplied diopter by the difference between finished reading glasses's O.C. and wearer's P.D. Consequently, We found out that the finished reading glasses counter to the German RAL-RG 915 policy. And We also found that it is relative to the diopter of lenses. In conclusion, based on the researched that wearing finished reading glasses have a dangerous factor for our vision. Therefore optician must need to recommend correctly made dispensing reading glasses based on the optical center point.

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Properties of Perovskite Materials and Devices Fabricated Using the Solvent Engineered One-Step Spin Coating Method (단일 스텝 스핀 코팅 방법에서 증발 제어 공정 변경에 따른 페로브스카이트 박막 물성 및 태양 전지 소자 특성 변화에 관한 연구)

  • Oh, Jungseock;Kwon, Namhee;Cha, DeokJoon;Yang, JungYup
    • New Physics: Sae Mulli
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    • v.68 no.11
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    • pp.1208-1214
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    • 2018
  • The one-step spin coating method is reported as an excellent thin film process because it can be easily used to fabricate high-quality methyl-ammonium lead tri-iodide ($MAPbI_3$) perovskite layers. One of the important things in the one-step spin coating method towards obtaining high-quality $MAPbI_3$ layers is the anti-solvent (AS) engineering, which consists of an one-step deposition of the $MAPbI_3$ film and dripping of the AS. The properties of the $MAPbI_3$ layer were found to be strongly influenced by the amount, dispensing speed, and spraying time of the AS solution. The $MAPbI_3$ solution was prepared by dissolving lead iodide and methyl-ammonium iodide in N,N-dimethylformamide and adding N,N-dimethyl sulfoxide. Diethyl ether (DE) was used for the AS solution. The results indicate that a $MAPbI_3$ layer appropriately sprayed with DE is beneficial for improving film quality and device efficiency because nucleation of $MAPbI_3$ layer is affected by the characteristics of DE, which affect the film's crystallinity, density, and surface morphology. The $MAPbI_3$ layer, which was optimized by using 0.7 mL of DE, a 3.03 mL/sec dispensing speed, and a 7 second time to spray after spinning showed the best efficiency of 13.74%, which was reproducible.

The Optimization of LCD Color Filter Coating Method

  • Cho, Moon-Chul;Bae, Dong-Ho
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2009.11a
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    • pp.177-177
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    • 2009
  • We examine the process to enhance the productivity of the thin-film transistor-addressed liquid-crystal display (TFT LCD) panels with the objective of optimizing the relation between the Type of color PR dispense nozzle and the amount of dispensing of color PR consumption, directly affecting a spectroscopic analysis. Most manufacturers of the panels have been utilizing a spin-type coater. We show that we successfully optimize the spectral values by controlling the color PR dispense type(Static dispense or Dynamic dispense) and the amount of color PR. From this study, we accomplished to decrease 43% in color PR consumption and to decrease 30% in color PR Stained, to decrease 30% rework rate.

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Fabrication of spiral scaffolds with nano-etched surface by using an innovative 3D printing method (혁신적인 3D 프린팅 방법을 사용하여 나노-에칭된 표면을 갖은 나선형 세포담체 제작)

  • Yang, Ji-Hun;Lee, Jae-Yun;Kim, Geun-Hyeong
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2018.06a
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    • pp.73-73
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    • 2018
  • 조직재생공학은 조직이나 장기를 재생하고 유지하는 데 초점을 맞춘 종합 분야이다. 세포담체는 세포가 조직이나 장기로 발달 할 수 있도록 결정적인 역할을 한다. 따라서 공극률, 기공 크기, 기공 상호 연결성, 표면 거칠기, 기계적 강도 및 기하학과 같은 기본 요구 사항들은 중요한 특성으로 간주된다. Particle leaching, phase separation, solvent casting, gas foaming, selective laser sintering, fused deposition 및 3D dispensing (printing)과 같은 다양한 Rapid Prototyping 방법이 세포담체 제조에 사용되었다. 또한, 다양한 천연 및 합성 고분자가 세포담체를 제조하는데 사용되어왔다. 본 연구에서는 기존의 3D 프린팅 방법과 플라즈마 에칭 공정을 이용하여 나노 에칭 된 나선형 가닥으로 구성된 3 차원 세포담체를 제작 하였다. 제작 된 세포담체의 물리적 및 생물학적 성질을 비교 연구하기 위해, 본 연구에서는 매끄러운 가닥을 대조물로 사용하였다. 나노 에칭된 표면은 초기 세포 부착, 증식 및 골 형성 분화와 같은 세포 활동에 영향을 미쳤다.

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Experimental Study on Spin Coated Thin Cover Layer for High Numerical Aperture Optical Disc

  • Dohoon Chang;Myongdo Ro;Duseop Yoon;Park, Insik;Dongho Shin;Kim, Jinhwan
    • Macromolecular Research
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    • v.9 no.6
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    • pp.313-318
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    • 2001
  • The present study relates to a method of manufacturing 100$\mu\textrm{m}$ thick cover layer for the high density digital versatile disc system (HD-DVD), which uses a high numerical aperture of 0.85 at 405 nm wavelength. Spin coating technique was investigated as means for manufacturing the cover layer within sufficient margins of thickness variation and with good mechanical properties including small radial and tangential tilts. The influence of processing variables such as spinning speed, spinning time, and dispensing position was investigated. The effect of viscosity of UV-curable resin was also investigated.

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Study on Correlation-based Feature Selection in an Automatic Quality Inspection System using Support Vector Machine (SVM) (SVM 기반 자동 품질검사 시스템에서 상관분석 기반 데이터 선정 연구)

  • Song, Donghwan;Oh, Yeong Gwang;Kim, Namhun
    • Journal of Korean Institute of Industrial Engineers
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    • v.42 no.6
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    • pp.370-376
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    • 2016
  • Manufacturing data analysis and its applications are getting a huge popularity in various industries. In spite of the fast advancement in the big data analysis technology, however, the manufacturing quality data monitored from the automated inspection system sometimes is not reliable enough due to the complex patterns of product quality. In this study, thus, we aim to define the level of trusty of an automated quality inspection system and improve the reliability of the quality inspection data. By correlation analysis and feature selection, this paper presents a method of improving the inspection accuracy and efficiency in an SVM-based automatic product quality inspection system using thermal image data in an auto part manufacturing case. The proposed method is implemented in the sealer dispensing process of the automobile manufacturing and verified by the analysis of the optimal feature selection from the quality analysis results.