• 한국임상수의학회지
• /
• 제25권4호
• /
• pp.303-306
• /
• 2008

Unknown age, spayed female mixed dog was presented with a severe lethargy. Radiography, ultrasonography, and blood screen test were performed to make a diagnosis. There were no specific radiographic findings. On the ultrasonography, small amount of ascites was found around gallbladder and hepatic vein was dilated approximately 6.1 mm. Blood screen test revealed a severe anemia. Result of heartworm ELISA kit was positive. At necropsy, parasites were in the left atrium, right ventricles, aorta, pulmonary artery, right renal artery.

• 대한수의학회지
• /
• 제41권1호
• /
• pp.79-83
• /
• 2001

Dirofilaria immitis parasitizes mainly in the pulmonary arteries and in the heart of dogs and cats. The parasite is also of public health importance, because it often elicits nodules in the pulmonary parenchyma and in the subcutaneous tissues, or sometimes parasitize itself in the eyes of human. In this study, we investigated the prevalence of heartworm infection among 165 dogs on three breeding farms in the vicinity of Seoul, Korea. Of 165 dogs, 83 dogs (50.3%) were infected with the parasite, as revealed by an antigen-detecting test using the peripheral blood. Of these, 23 dogs (20.2%) contained microfilaria using the peripheral blood, which are potential source of transmission to uninfected animals and to humans in the endemic area. None of infected dogs showed any clinical signs associated with the disease. Since the three farms were located in the vicinity of Seoul, the unexpectedly high infection rate could imply that the possibility of exposure of both animals and humans living in the metropolitan Seoul area to the parasite is higher than in the other area of Korea.

• Parasites, Hosts and Diseases
• /
• 제51권6호
• /
• pp.645-650
• /
• 2013

A simple, rapid, and high-throughput method for detection and identification of Wuchereria bancrofti, Brugia malayi, Brugia pahangi, and Dirofilaria immitis in mosquito vectors and blood samples was developed using a real-time PCR combined with high-resolution melting (HRM) analysis. Amplicons of the 4 filarial species were generated from 5S rRNA and spliced leader sequences by the real-time PCR and their melting temperatures were determined by the HRM method. Melting of amplicons from W. bancrofti, B. malayi, D. immitis, and B. pahangi peaked at $81.5{\pm}0.2^{\circ}C$, $79.0{\pm}0.3^{\circ}C$, $76.8{\pm}0.1^{\circ}C$, and $79.9{\pm}0.1^{\circ}C$, respectively. This assay is relatively cheap since it does not require synthesis of hybridization probes. Its sensitivity and specificity were 100%. It is a rapid and technically simple approach, and an important tool for population surveys as well as molecular xenomonitoring of parasites in vectors.

• 한국임상수의학회지
• /
• 제27권6호
• /
• pp.631-634
• /
• 2010

본 연구는 대전시와 강원도에서 사육중인 개 252두 (암컷: 127두, 수컷: 125두, 실외견: 147두, 실내견: 105두)를 대상으로 ELISA kit (SNAP 4Dx, IDEXX Lab. USA)를 이용하여 심장사상충증, 아나플라즈마증, 보렐리아증 및 얼리키아증에 대한 혈청학적 방법으로 발생율을 조사하였다. 아나플라즈마증은 암컷에서 실내견보다 실외견에서 유의성 있는 높은 감염율을 나타내었다. 또한 얼리키아증에서는 연령별로 분석한 결과, 실외견에서 실내견보다 4세미만, 4-7세사이, 7세 이상에서 각각 유의성 있는 높은 감염율을 나타내었다. 본 연구는 임상가들에게 유용한 임상자료로 활용될 것으로 판단된다.

• 대한수의학회지
• /
• 제43권3호
• /
• pp.507-516
• /
• 2003

This study was performed to clarify the induction possibility of anaphylactoid reactions by the administration with the heartworm extracts, and, if any, to elucidate different virulences in terms of the protein concentrdtions and sexes of Dirofilaria immitis. Twenty three clinically healthy D. immitis-free adult dogs were used in the present study. The experimental animals were divided into 5 groups. Group A (5 heads) was administered with an female heartworm extract containing 0.1 g/dl protein concentration. Group B (4 heads) was administered with an male heartworm extract containing 0.1g/dl protein concentration. Group C (5 heads) was administered with an female heartworm extract containing 0.2 g/dl protein concentration. Group D (4 heads) was administered with an male heartworm extract containing 0.2 g/dl protein concentration. Group E (5 heads) was administered with an female heartworm extract containing 0.4 g/dl protein concentration. The changes of clinical symptoms and vital signs (body temperature, heart rate and respiration rate) were examined before and 5 minutes, 15 minutes, 30 minutes, 1 hour, 2 hours and 24 hours after injection with the extracts, respectively. In addition, the changes of hematological values (RBC, PCV and total leukocytes counts), serum chemical values (ALP and CK) were determined. It was considered that heartworm extract could induce anaphylactoid reaction and adult female heartworm extract was more affective than those of adult male heartworm extract in the changes of clinical symptoms, vital signs, hematological values and serum chemical values.

• 한국임상수의학회:학술대회논문집
• /
• 한국임상수의학회 2008년도 추계학술대회
• /
• pp.147-147
• /
• 2008

• 한국임상수의학회:학술대회논문집
• /
• 한국임상수의학회 2008년도 추계학술대회
• /
• pp.148-148
• /
• 2008

• 한국임상수의학회:학술대회논문집
• /
• 한국임상수의학회 2007년도 추계국제학술대회 및 컨퍼런스
• /
• pp.626-626
• /
• 2007

• 한국임상수의학회지
• /
• 제22권2호
• /
• pp.79-83
• /
• 2005

본 연구에서는 주거환경이 다른 6두의 개를 대상으로 일정한 시간간격으로 채혈하여 만든 염색표본에서 필라리아자충의 수를 측정하여 정기 출현성을 파악하고자 trigonometric모형을 사용하였다. 모형에 근거하여 계산된 주기성 지수 (periodicity index)는 평균 $57.6\%$ (범위: $25.6-95.5\%$)로 나타났다. 혈액 내 필라리아자충의 수가 최고에 이르는 시점은 21:00 (범위: 20:04-21:29), 최저에 이르는 시점은 09:00 (범위: 08:04-09:29)로 나타났다. 실체로 관찰된 자충의 수와 모형에 의하여 계산된 기대치와의 상관계수는 $6.4-49.2\%$로 자충의 수에 따라 차이를 보였다. 본 연구에 사용된 6두의 실험견 모두 nocturnally sub-periodic 양상을 보였다. 이러한 결과는 기존의 연구에서 보고된 혈중 자충의 수가 최대에 도달하는 시간은 동일하였지만 최저에 이르는 시간은 차이가 있는 것으로 나타났다 국내 개에서 조사 대상지역에 따른 정기 출현성에 차이가 없는지를 명확히 증명하고 심장사상충의 역동성을 보다 정확하게 이해하기 위해서는 광범위한 조사가 필요할 것으로 사료된다.

• Parasites, Hosts and Diseases
• /
• 제51권5호
• /
• pp.569-572
• /
• 2013

Dirofilariasis is a rare disease in humans. We report here a case of a 48-year-old male who was diagnosed with pulmonary dirofilariasis in Korea. On chest radiographs, a coin lesion of 1 cm in diameter was shown. Although it looked like a benign inflammatory nodule, malignancy could not be excluded. So, the nodule was resected by video-assisted thoracic surgery. Pathologically, chronic granulomatous inflammation composed of coagulation necrosis with rim of fibrous tissues and granulations was seen. In the center of the necrotic nodules, a degenerating parasitic organism was found. The parasite had prominent internal cuticular ridges and thick cuticle, a well-developed muscle layer, an intestinal tube, and uterine tubules. The parasite was diagnosed as an immature female worm of Dirofilaria immitis. This is the second reported case of human pulmonary dirofilariasis in Korea.