• 제목/요약/키워드: Direct seeding

검색결과 420건 처리시간 0.028초

생쥐 2세포기배의 동결보존 (Cryopreservation of Mouse 2-Cell Embryos)

  • 백청순;서병희;이재현;이경광
    • Clinical and Experimental Reproductive Medicine
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    • 제16권1호
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    • pp.9-14
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    • 1989
  • For the cryopreservation of human embryos this study was accomplished as a preliminary experiment. The purpose of this study is to obtain optimal cryoprotectant, addition and dilution method of cryoprotectant and cooling rate for raising survival of frozon and thawed 2-cell mouse embryos. Seeding was done at $-7^{\circ}C$ and the straw contained embryos was plunged at $-30^{\circ}C$ when the slow cooling was ended. Embryos those developed normally to blastocyst after in vitro culture for over 96 hours were regarded as survival ones. The survival was the rate of number of survival embryos against the recovered embryos. The results are followed : 1. The survivals were 6.3, 71.2 and 67.4% respectively, when Glycerol, DMSO and 1,2-Propanediol were used as cryoprotectant. 2. When sucrose was added in freezing solution, the survival was 69.0%. That was higher than the survival of embryos frozen without sucrose in freezing solution. The difference was not significant. 3. Addition and dilution of cryoprotectant by 4 stepwise raised the survival than by direct, but that was not significant. 4. When embryos were frozen by -0.3, -0.5 and $-1^{\circ}C/min$ before plunged into $LN_2$, the survivals were 67.9, 78.0 and 37.0% respectively. The differnce was significant.

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Reproduce results on seed priming effect of indoor experiments in the field

  • Nakao, Yoshihiro;Tsujimoto, Yasuhiro;Katsura, Keisuke;Sone, Chiharu;Sakagami, Jun-Ichi
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.334-334
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    • 2017
  • Unstable soil moisture conditions often negatively affect the emergence, seedling establishment, and growth uniformity at the initial stage, and then reduce the grain yield and biomass in direct seeding cultivation for rice in rainfed fields. Therefore it is important to develop a technique to increase the rapid and stabilized seedling establishment and improve the uniformity of initial growth after sowing. This study aims to confirm results on seed priming effect of indoor using petri dish experiments can be reproduced in the field using container at Ghana. Twenty-seven rice varieties including of Oryza sativa L. and O. glaberrima Steud. were used in this study. The experiments using petri dish and container with different soil moisture conditions (5%, 10%, 15%, 20%) were compared. As a result, a significant positive correlation was found between the germination time uniformity in the primed seed of petri dish and emergence time uniformity in the primed seed of container in 10% and 15% soil moisture condition. A significant positive correlation was found between the germination time uniformity in the primed seed of petri dish and plant height in the primed seed of container in 10% soil moisture condition in O. glaberrima. This study concluded that the priming effect in petri dish demonstrate those in container in the field condition of Ghana in 10% and 15% soil moisture condition during seedling stage.

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Reproduce results on seed priming effect of indoor experiments in the field

  • Nakao, Yoshihiro;Tsujimoto, Yasuhiro;Katsura, Keisuke;Sone, Chiharu;Sakagami, Jun-Ichi
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.53-53
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    • 2017
  • Unstable soil moisture conditions often negatively affect the emergence, seedling establishment, and growth uniformity at the initial stage, and then reduce the grain yield and biomass in direct seeding cultivation for rice in rainfed fields. Therefore it is important to develop a technique to increase the rapid and stabilized seedling establishment and improve the uniformity of initial growth after sowing. This study aims to confirm results on seed priming effect of indoor using petri dish experiments can be reproduced in the field using container at Ghana. Twenty-seven rice varieties including of Oryza sativa L. and O. glaberrima Steud. were used in this study. The experiments using petri dish and container with different soil moisture conditions (5%, 10%, 15%, 20%) were compared. As a result, a significant positive correlation was found between the germination time uniformity in the primed seed of petri dish and emergence time uniformity in the primed seed of container in 10% and 15% soil moisture condition. A significant positive correlation was found between the germination time uniformity in the primed seed of petri dish and plant height in the primed seed of container in 10% soil moisture condition in O. glaberrima. This study concluded that the priming effect in petri dish demonstrate those in container in the field condition of Ghana in 10% and 15% soil moisture condition during seedling stage.

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Effect of Crop Establishment and Weed Control Method on Productivity of Transplanted aman Rice

  • Ali, Mohammad;Haque Bir, Md. Shahidul;Rahman, Md. Habibur;Ayesha, Sultana Kaniz;Hoque, Aminul;Harun-Ar-Rashid, Md.;Islam, Md. Rashidul;Park, Kee Woong
    • Weed & Turfgrass Science
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    • 제7권2호
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    • pp.120-129
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    • 2018
  • This experiment was conducted to find the most suitable crop establishment method and weed management practices for transplanted aman rice in Bangladesh. Rice variety Bangladesh Rice Research Institute (BRRI) dhan44 was used as planting materials where three crop establishment methods ($T_1$: direct wet seeding by drum seeder; $T_2$: hand broadcasting; $T_3$: transplanting) and four weeding options ($W_1$: Hand weeding (HW); $W_2$: BRRI weeder+HW; $W_3$: Herbicide+HW; and $W_4$: no weeding) were tested. Among the crop establishment methods, the highest grain yield ($5.12t\;ha^{-1}$) was obtained with the $T_3$, while the highest benefit cost ratio (BCR) of 2.08 was found in $T_2$. In case of the weed management method, $W_1$ showed superior results on the plant $density/m^2$ (139.66) at 60 days after transplanting (DAT), grain yield ($4.97t\;ha^{-1}$), and BCR (2.03). On the other hand, the highest plant dry matter (36.20 g) at 60 DAT and the highest yield ($6.10t\;ha^{-1}$) were obtained in a $T_3W_1$ combination. The results of this study show that the productivity of rice during aman season could be most significantly increased with the use of transplanting ($T_3$) alone, hand weeding ($W_1$) alone, or a combination of the two methods ($T_3W_1$).

경북지역 논 잡초 발생분포 조사 (A Survey of Weeds Occurrence on Paddy Fields in Gyeongbuk Province in Korea)

  • 김상국;김학윤
    • Weed & Turfgrass Science
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    • 제3권1호
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    • pp.6-12
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    • 2014
  • 본 조사는 2013년에 경북지역 13개 시 군의 320지점에서 논 잡초 발생 현황을 파악하기 위하여 수행되었다. 경북지역의 논잡초 조사결과, 27개과(family) 51초종(species)이었고 과별로는 벼과(family) 9초종, 사초과(family) 5초종, 마디풀과(family) 4초종, 국화과(family) 4초종 순으로 나타났다. 발생 잡초종의 생활형태는 일년생잡초 34종, 다년생잡초 17종으로 대부분 일년생잡초였다. 경북지역의 우점잡초는 물달개비(10.80%) > 좀개구리밥(10.74%) > 가막사리(8.77%) > 강피(7.17%) > 올챙이고랭이(6.20%) > 올방개(6.13%) > 벗풀(5.85%) 순이었다. 물달개비, 좀개구리밥, 가막사리, 강피, 올챙이고랭이, 올방개, 벗풀의 우점도는 전체 잡초종의 50%이상 차지하였고 TWINSPAN 분석을 통한 지역별 잡초종의 유사성은 여뀌바늘, 올챙이고랭이, 미국외풀, 마디풀, 뚝새풀에 의해 구분되어 추후 경북지역 논 잡초의 발생 양상을 예측하며 잡초군락의 변화를 파악함으로써 체계적인 논 잡초 관리방안을 마련할 수 있을 것으로 판단되었다.

토마토 초기 생장에 미치는 키틴 처리의 영향 (Effect of Chitin Application on the Early Growth of Tomato)

  • 김옥란;지명심;김길용;차규석;채동현;박노동
    • Applied Biological Chemistry
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    • 제47권3호
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    • pp.361-365
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    • 2004
  • 키틴, 키토산 및 그 유도체의 토양처리와 종자 피복 등이 토마토 생육에 미치는 효과를 조사하고 분석하였다. 키틴과 그 유도체의 토양 처리는 토마토 초기 생육을 촉진하였으며, 키틴과 키토산을 토양에 혼화 처리한 경우 그 효과가 가장 탁월하였다. 이들의 처리는 토마토 근부의 생육을 촉진하였다. 토마토 식물체 중의 무기성분을 분석하여 이들의 처리효과를 분석하였던 바, 키틴 키토산의 처리는 질소와 칼륨의 흡수를 촉진하였으며 칼슘의 흡수를 억제하였다. 그러므로 이들은 식물의 무기양분 흡수의 조절을 통하여 생육을 촉진하는 것으로 판단하였다.

Weed Occurrence, Rice Growth, and Soil Temperature as Affected by Different Biodegradable Mulching Materials in Wet Seeded Rice

  • Yang, Woon-Ho;Kim, Jae-Hyun;Kim, Je-Kyu;Han, Hee-Suk;Shin, Jeong-Ju
    • 한국작물학회지
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    • 제48권1호
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    • pp.1-7
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    • 2003
  • In spite water direct seeding cultivation of rice has advantages in terms of laborsaving and cost-down, it demonstrates uneven seedling establishment and difficult weed management. To select an applicable mat for mulching system of water seeded rice, weed occurrence, growth and yield performances of rice, and soil temperature were evaluated in several kinds of biodegradable mulching material. Field studies were conducted at the experimental farm of National Crop Experiment Station in 1999 and 2000. Among the mats tested in 1999, Safer-mat had the greatest effect on inhibiting weed occurrence and Lawn-mat demonstrated the least efficacy of weed control (EWC) value. In Safer-mat treatment, Ludwigia prostrata was the dominant weed species but Echinochloa crus-galli was not observed. Different kinds of mulching material from the mats studied in 1999 were tested in 2000. Biodegradable polyester (PES) coated on the recycled paper for newspaper press (RP) treatments showed similar or higher EWC values than non-mulched broadcast seed rice with 2 times of herbicide application (BC-herbicide) and Safer-mat that was the most effective mat on preventing weed appearance in 1999. Immediately after mat mulching on the surface of paddy soil, five pregerminated rice seeds were manually sown in each 3cm-hole formerly punctured at 30${\times}$14 cm spacing in 2000. All the 3 kinds of mulching material tested in 2000 had better seedling stand than BC-herbicide treatment. Rice yield in PES coated with 10 thickness on RP (PES10 $\mu\textrm{m}$+RP) was a little higher than in BC-herbicide treatment but the former produced less panicles in unit land area than the latter. PES10 $\mu\textrm{m}$+RP demonstrated a great effect on raising soil surface temperature than non-mulching treatment throughout the period of seedling establishment.

한국 재래 적미 수집 및 특성 검정 V. 수심 및 토심에 따른 발아 특성 (Collection and Evaluation of Korean Red Rices V. Germination Characteristics on Different Water and Soil Depth.)

  • 서학수;하운구
    • 한국작물학회지
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    • 제38권2호
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    • pp.128-133
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    • 1993
  • 한국 재래 적미(앵미)는 다양한 유전적 변이와 작물학적 특성이 있을 것으로 추측되어 유전자측으로서의 유용성을 검정하고자 경상남북도에서 수집한 적미중 생웅적으로 특징있는 장입형 적미 10계통, 단입형 적미 10계통 등 20계통과 대조품종으로 삼강벼, 섬진벼를 공시하여 수확후 저장기한에 따른 휴면정도, 수중발아 및 토중발아 등 발아특성을 검정한 결과를 요약하면 다음과 같다. 1. 한국 재래 장. 단입형 적미는 수확직후 99%이상의 발아율을 보여 휴면성이 거의 없었다. 2. 장입형 적미는 수심 7cm에서 83.5%의 식물체의 묘 출현율을 보였고 단입형 적미는 수심 11cm에서 83.5%의 묘 출현율을 보여 실용상 지장이 없는 입묘율이 확보되었으나 대조품종인 삼강벼는 수심 7cm이상에서 섬진벼는 수심 11cm 이상에서 전혀 출현하지 못하였다. 3. 토심 7cm에서 장입형 적미는 71.7% 단입형 적미는 73.9%의 묘 출현율을 보였으며 대조품종인 삼강벼와 섬진벼는 각각 40.0%, 28.0%를 보였다. 4. 한국 재래 적미는 직파재배의 유용 유전자측으로 이용 가능성이 높은 것으로 평가 되었다.

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무인헬기 부착용 원심식 볍씨 살포 장치에 관한 연구 (Investigation of Centrifugal Rice Seeder for Unmanned Helicopter)

  • 강태경;김승희;전현종;최덕규;이채식;최용;백남현
    • Journal of Biosystems Engineering
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    • 제37권6호
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    • pp.335-341
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    • 2012
  • Purpose: While an unmanned helicopter has been extensively used for spraying chemicals on agricultural crops, its low utilization (two months per year from July to August) has recently become an issue. This study aimed to increase the utilization of the unmanned helicopter. The centrifugal rice seeder, a mounting device for rice seeding for unmanned helicopter was developed and assessed its performance. Methods: The concept of the centrifugal spraying device was to obtain design criteria for centrifugal distribution. Four types of blade shapes namely straight, curved, straight wing and curved wing were developed and used. The rotational speed of the blades was tested at 1,000, 1,200 and 1,400 rpm. Results: The blade shapes, rotational blade speed and angle of trajectory were theoretically analyzed and results were validated with a series of laboratory experiments. Conclusions: The curved wing blades provided the distribution uniformity (DU) at 1,200rpm of rotational speed and 60 degree of seed drop point. The spray uniformity of 4.2% was also achieved.

오갈피나무의 육묘이식재배(育苗移植栽培)에 따른 생육특성(生育特性) (Growth Characteristics of Acanthopanax sessiliflorus Seem Seedling Grown under Different Conditions and Seasons)

  • 조선행
    • 한국약용작물학회지
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    • 제7권1호
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    • pp.22-26
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    • 1999
  • 오갈피나무의 묘목(苗木)을 실생(實生)으로 대량생산 체계를 확립하기 위한 일환으로 하우스를 이용한 육묘이식재배(育苗移植栽培) 시험을 수행하면서 출아율(出芽率), 활착률(活着率), 득묘율(得苗率) 및 생육특성(生育特性)을 조사하였다. 출아율(出芽率)은 하우스내 파종에서 약 90%의 수치를 보였으나 노지직파(露地直播)에서는 약 40%로 나타났으며, 이식할 때 본엽수(本葉數)에 따른 활착률(活着率)은 본엽 $2{\sim}3$매에서 100%이었으나 그보다 적거나 많으면 감소하였다. 그리고 이식시기에 따른 활착률(活着率)은 4월 하순부터, 5월 초순에는 100%로 나타났으나 그보다 이르거나 늦으면 감소하였다. 또한 득묘율(得苗率), 수고(樹高), 근장(根長) 및 생체중(生體重)은 노지직파재배구(露地直播栽培區)보다 육묘이식재배구(育苗移植栽培區)에서 모두 높은 수치를 보였다.

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