• Food Science and Biotechnology
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• 제18권6호
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• pp.1447-1458
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• 2009

Volatile extracts obtained from traditional Chinese-type soy sauces prepared with soybean (SSSB) and defatted soy meal (SSDSM) by solid phase microextraction (SPME) and direct solvent extraction (DSE) were analyzed by gas chromatography-mass spectrometry (GC-MS). The volatile flavor compounds and relative contents of different chemical classes detected in SSSB and SSDSM were compared for their differences. Results showed that significant differences in both constituents of volatile flavor compounds and relative contents of different chemical classes were observed for both kinds of soy sauces. A total of 152 and 131 compounds were identified in SSSB and SSDSM, respectively, and 102 volatile flavor compounds were common in both kinds of soy sauces. Moreover, relative contents of acids, aldehydes, esters, furan(one)s, miscellaneous compounds, phenols, pyrazines, pyrrol(idinon)es, and sulfur-containing compounds in both kinds of soy sauces were all significantly different.

• 대한전기학회논문지:전기기기및에너지변환시스템부문B
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• 제52권11호
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• pp.563-571
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• 2003

In this paper, the control algorithm and control method for a combined system of shunt passive filter and series active filter in 3-phase 4-wire system are discussed. Moreover, the three-phase four-wire system is widely employed in distributing electric energy to several office building and manufacturing plants. In such systems, the third harmonic and its 3th harmonics are termed as triple and zero sequence components that do not cancel each other in the system neutral. Consequently, the triple harmonics add together creating a primary source of excessive neutral current. Regarding this concern, this paper presents a new control algorithm for a series hybrid active system, whereas the control approach it adopts may directly influence its compensation characteristics. Hence, the advantage of this control algorithm is direct extraction of compensation voltage reference and the required rating of the series active filter is much smaller than that of a conventional shunt active filter. Some experiments were executed and experimental results from a prototype active power filter confirm the suitability of the proposed approach.

• 미생물학회지
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• 제38권2호
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• pp.103-106
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• 2002

사상균류의 세포자체로부터 직접 PCR을 하는 방법은 세포벽 파괴의 어려움 때문에 모든 곰팡이에 적용될 수 없다. 극초단파 조사는 사상균류의 DNA를 추출함에 있어서 그 유용함이 이미 검중된 바 있는데, 본 논문에서는 극초단파 조사를 이용하여 Aspergillus nidulans의 포자로부터 손쉽게 주형 DNA를 얻어 PCR증폭하는 방법을 소개하고자 한다. 본 실험에서는 극초단파 조사시간과 PCR에 필요한 주형 DNA의 양 등을 최적화하였으며, 이렇게 수행된 PCR결과는 single copy유전자를 대상으로도 약 3 kb크기의 산물가지 증폭 가능하여, 형질전환체를 선별하기에 충분한 크기의 산물들도 효과적으로 얻어짐을 보여주었다. 따라서 이 방법은A. nidulans의 형질전환체를 보다 손쉽게, 시간을 절약하여 선별할 수 있는 효과적인 방법이라 생각된다.

• Journal of Korean Society for Atmospheric Environment
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• 제9권E호
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• pp.373-381
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• 1993

To understand the human exposure levels of volatile halogenated hydrocar-bons in ambient air, a new rapid and convenient analytical method for determination of the compounds in gaseous phase was evaluated and established. The method is based upon passsive diffusion to personal sampler containing adsorbent and solvent extraction followed by purge trap/ on-column cryof-ocusing method. A new method needs no special instrumentation for gas collection because it is based upon the passive diffusion principle. The typical chromatogram obtained in this study proved that rapid and simultaneous determination of target analytes was possible with good resolution. The developed method was successfully applied to determine the volatile halogenated hydrocarbons in indoor air and the values obtained by this new method were compared with those by direct suction method. The concentration of carbon tetrachloride, 1,1,2-trichloroethylene, chloroform showed the values below 400$\mug/m^3$ except the maximum of 1,513$\mug/m^3$ of chloroform. 1,1,1-Trichloroethane showed approximately 1,000 to 5,000$\mug/m^3$ range of diurnal fluctuation in indoor air.

• 한국표면공학회지
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• 제37권4호
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• pp.220-225
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• 2004

A new ion beam extraction system is designed using a simple ion mass filter and a micro mass balance and a QMS based detecting system. A quadrupole Mass Filter is used for selective ion beam formation from inductively coupled high density plasma sources with appropriate electrostatic lens and final analyzing QMS. Also a quartz crystal microbalance is set between a QMF and a QMS to measure the etching and polymerization rate of the mass selected ion beam. An inductively coupled plasma was used as a ion/radical source which had an electron temperature of 4-8 eV and electron density of $4${\times}$10^{11}$＃/㎤. A computer interfaced system through 12bit AD-DA board can control the pass ion mass of the qmf by setting RF/DC voltage ratio applied to the quadrupoles so that time modulation of pass ion's mass is possible. So the direct measurements of ion - surface chemistry can be possible in a resolution of $1\AA$/sec based on the qcm's sensitivity. A full set of driving software and hardware setting is successfully carried out to get fundamental plasma information of the ICP source and analysed $Ar^{+}$ beam was detected at the $2^{nd}$ QMS.

• 정보학연구
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• 제7권3호
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• pp.113-118
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• 2004

기존의 누설전류기법에서 한층 진일보된 비접촉식 수신용 안테나를 이용하여 복잡한 지역과 광범위한 지역에서 효율적인 고분자 애자의 오손정도를 진단하기 위하여 본 연구를 착수하였다. 착수된 연구는 가공된 챔버 내 설치된 고분자애자에 인위적으로 카올린과 소금을 혼합후 증류수에 혼합하여 인공 오손실시 후 방사전자파와 스페트럼 그리고 부분방전전하량을 동시에 측정 후 전자파 신호에 대한 카올린의 오손정도에 대한 특징추출을 위하여 다우비치 이산웨이블렛 변환기법에 적용 후 각각의 통계적인 파라미터의 특징을 추출하였다.

• International Journal of Naval Architecture and Ocean Engineering
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• 제9권1호
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• pp.25-34
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• 2017

The concentric dual-buoy Wave Energy Converter (WEC), which consists of external buoy (hallow-cylinder) with toroidal appendage and cylindrical internal buoy within the moon-pool is suggested in this research and its performance in various wave conditions is studied. The Linear Electric Generator (LEG), consisting of a permanent magnet and coils, is used as a direct Power Take-Off (PTO) system. To maximize the electrical energy extracted from the PTO system, the relative heave motions between the dual buoys must be highly amplified by the multiple resonance phenomena of dual-buoy and internal-fluid motions. The high-performance range can be widened by distributing those natural frequencies with respect to the peak frequency of the wave spectrum. The performance of the newly developed dual-buoy WEC was measured throughout the systematic 1:5.95-model test in regular and irregular waves conducted in a wave tank at Seoul National University. The model-test results are also validated by an independently developed numerical method.

• Animal cells and systems
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• 제3권1호
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• pp.73-78
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• 1999

Although there are several methods for the preparation of mitochondrial DNA (mtDNA) from mammalian tissues, most are relatively long ultracentrifugation or manipulations by a small-scale method. We escribed a rapid method for large-scale extraction of mtDNA from human placental and horse liver tissues. The method is based on the preparation and homogenization of tissues, urification of crude mitochondria by differential centrifugations and isolation of mtDNA by alkaline Iysis. It was improved from Pre-existing methods by replacing some steps with simpler ones and discarding many others. This method gives a high yield of pure mtDNA(approximately 1-5mg from one placenta; ca. 400-600 g wet weight), depending on its sources (fresh tissue gave better results than frozen one). The resulting mtDNA indicated that this method can yield mtDNA in sufficient purity and quantity to identify the direct restriction analysis on agarose gel, random-primed labeling as a probe, and end labeling. Therefore, the method is ideal for obtaining good mtDNA samples to conduct routine restriction fragment length polymorphism (RFLP) analyses of natural populations for genetic studies.

• 정보처리학회논문지:소프트웨어 및 데이터공학
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• 제5권7호
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• pp.333-338
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• 2016

본 연구의 목적은 수삼 등급을 판정하는 기준 중에서 수삼의 중량을 직접 측정하지 않고 영상 분석으로 가능한 근접하게 추정하는 것이다. 이를 위해 수삼영상 취득장치를 제작하였으며 126개의 수삼샘플을 대상으로 영상을 취득하였다. 각각의 수삼샘플의 중량을 측정하고 영상분석 데이터를 이용하여 중량추정공식을 산출하는데 이용하였다. 영상분석과 파라미터 추출과정에는 C언어 기반의 Labwindows/CVI 개발 툴과 오픈소스 라이브러리 OpenCV를 이용하였다. 영상분석 과정에서 추출한 파라미터와 중량과의 상관관계를 가장 잘 표현할 수 있는 필터설정 값을 추적하여 적용하였고, 최소제곱법을 사용한 선형 회귀분석으로 선형성을 가지는 0.9162의 강한 양의 상관계수 값을 얻을 수 있었다.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제5권1호
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• pp.101-107
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• 2002

We experienced two cases of Rotor syndrome in brothers who were a 13 year-old boy and an 11 year-old boy, respectively. They presented with icteric scleras for a few months. Their common laboratory characteristics were as follows: Direct bilirubin was more increased than indirect bilirubin, but aminotransferases were normal. Plasma indocyanine green (ICG) test revealed hepatic excretory defect: plasma ICG concentrations 15 minutes after intravenous injection were 80.45% and 78.28%, respectively. 99mTc-DISIDA Hepatobiliary scan showed that severely decreased hepatic extraction with mild cardiac blood pool, markedly delayed biliary excretion in both intra- & extra- hepatic bile ducts, delayed visualization of gall bladder, and markedly delayed intestinal biliary passage. Needle liver biopsy showed normal hepatic histology without pigmentation.