• Title/Summary/Keyword: Dioscorea opposite

Search Result 3, Processing Time 0.078 seconds

Development of Hydroponic Culture System for Seed Tuber Production of Yam (Dioscorea opposita) (씨마 대량생산을 위한 수경재배 조건 연구)

  • Lee Hee-Sun;Kim Hyun-Jun;Park Byoung-Jae;Park Cheol-Ho;Chang Kwang-Jin
    • Korean Journal of Plant Resources
    • /
    • v.19 no.2
    • /
    • pp.232-236
    • /
    • 2006
  • This study was carried out to establish of hydroponic culture system for year-round mass production of yam and for supply of disease-free seed tubers from the superior yam species. There were not difference in tuber weight between 9 hrs photoperiod(640mg) and natural photoperiod(600mg). However, longer photoperiod than 9 hrs such as 12 hrs and 15 hrs decreased tuber weight to 490 and 500g, respectively, which sugested that long photoperiod showed adverse effect for tuber enlargement. Tuber enlargement of Dioscorea opposite according to ionic strength was higher at 50% nutrient of Sanyak's standard solution. The optimal concentration of NAA for the best tuber growth showed at 100mg/L as a 560mg while control showed a relatively lower tuber growth(350mg).

Suppression of VCAM-1 Expression in Human Aortic Smooth Muscle Cells Treated with Ethanol Extracts of Cynanchum wilfordii Radix, Arctium lappa L., and Dioscorea opposita (백수오, 우엉, 마 추출물 혼합비율에 따른 혈관부착인자 VCAM-1의 발현억제 효과)

  • Cho, Young-Mi;Song, Hae-Seong;Jang, Seon-A;Park, Dae-Won;Shin, Yu Su;Jeong, Yong Joon;Kang, Se Chan
    • Korean Journal of Plant Resources
    • /
    • v.29 no.5
    • /
    • pp.525-531
    • /
    • 2016
  • Cynanchum wilfordii Radix (CWR), Arctium lappa L (ALL), and Dioscorea opposite (DO) have been known to improve blood lipid profile, blood pressure, and inflammation. To find the optimal combination ratio of CWR, ALL, and DO in terms of vascular health improvement, we compared the effects of various combinations on gene expression of Vascular cell adhesion protein 1 (VCAM-1) in human aortic smooth muscle cells (HASMC). VCAM-1 mediates endothelial leukocyte adhesion and is upregulated in atherosclerosis. Cells was stimulated by TNF-α (10 ng/㎖, 2h) and treated with various combinations for 24 h. A combination (CADM5, CWR:ALL:DO = 2:1:1) showed the strongest suppression of VCAM-1 so that CADM5 was chosen for further experiments. We performed cell viability test with CADM5 (0, 3.125, 12.5, 25, 50, and 100 ㎍/㎖) and no cytotoxicity was found. We also investigated the effect of CADM5 on protein expression of VCAM-1, ICAM-1, Nrf-2, and HO-1 using western blotting. We found that CADM5 diminished the expression of VCAM-1 and increased the expression of Nrf-2 and HO-1. Therefore, we concluded that CADM5 (CWR:ALL:DO = 2:1:1) effectively improves vascular health by regulating the expression of VCAM-1.

Identification of Endogenous Gibberellins by Feeding of [$^{14}\textrm{C}$] $\textrm{GA}_{12}$ in Chinese Yam, Dioscorea opposite

  • Lee, In-Jung;Kim, Sang-Kuk;Lee, Sang-Chul;Lee, Bong-Ho;Jang, Soo-Won;Park, Tae-Shik
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.48 no.2
    • /
    • pp.68-72
    • /
    • 2003
  • The metabolism of [$^{14}\textrm{C}$] $\textrm{GA}_{12}$ in the Chinese yam (Dioscorea opposita Thunb. var. Tsukune) was examined to determine the identification of endogenous gibberellins. [$^{14}\textrm{C}$] $\textrm{GA}_{12}$ was metabolized to $\textrm{GA}_{53}$, $\textrm{GA}_{44}$, $\textrm{GA}_{19}$, $\textrm{GA}_{20}$, $\textrm{GA}_1$, $\textrm{GA}_8$, $\textrm{GA}_{15}$, $\textrm{GA}_{24}$, $\textrm{GA}_9$, $\textrm{GA}_{36}$ and $\textrm{GA}_4$. Radioactivity of GAs in non C-13 hydroxylation route was five-fold higher than that of early C-13 hydroxylation in analyzed GA-metabolites. Radioactivity of $\textrm{GA}_4$ was always four times higher than that of $\textrm{GA}_1$ at every feeding time. $\textrm{GA}_1$ radioactivity has always a lower level to below 200 DPM. The major pathway of endogenous GA metabolism in seedlings of the Chinese yam might be the non C-13 hydroxylation pathway.