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http://dx.doi.org/10.7732/kjpr.2016.29.5.525

Suppression of VCAM-1 Expression in Human Aortic Smooth Muscle Cells Treated with Ethanol Extracts of Cynanchum wilfordii Radix, Arctium lappa L., and Dioscorea opposita  

Cho, Young-Mi (Department of Oriental Medicine Biotechnology, Kyung Hee University)
Song, Hae-Seong (Department of Oriental Medicine Biotechnology, Kyung Hee University)
Jang, Seon-A (Department of Oriental Medicine Biotechnology, Kyung Hee University)
Park, Dae-Won (Department of Oriental Medicine Biotechnology, Kyung Hee University)
Shin, Yu Su (Department of Herbal Crop Research, NIHHS, RDA)
Jeong, Yong Joon (Department of Oriental Medicine Biotechnology, Kyung Hee University)
Kang, Se Chan (Department of Oriental Medicine Biotechnology, Kyung Hee University)
Publication Information
Korean Journal of Plant Resources / v.29, no.5, 2016 , pp. 525-531 More about this Journal
Abstract
Cynanchum wilfordii Radix (CWR), Arctium lappa L (ALL), and Dioscorea opposite (DO) have been known to improve blood lipid profile, blood pressure, and inflammation. To find the optimal combination ratio of CWR, ALL, and DO in terms of vascular health improvement, we compared the effects of various combinations on gene expression of Vascular cell adhesion protein 1 (VCAM-1) in human aortic smooth muscle cells (HASMC). VCAM-1 mediates endothelial leukocyte adhesion and is upregulated in atherosclerosis. Cells was stimulated by TNF-α (10 ng/㎖, 2h) and treated with various combinations for 24 h. A combination (CADM5, CWR:ALL:DO = 2:1:1) showed the strongest suppression of VCAM-1 so that CADM5 was chosen for further experiments. We performed cell viability test with CADM5 (0, 3.125, 12.5, 25, 50, and 100 ㎍/㎖) and no cytotoxicity was found. We also investigated the effect of CADM5 on protein expression of VCAM-1, ICAM-1, Nrf-2, and HO-1 using western blotting. We found that CADM5 diminished the expression of VCAM-1 and increased the expression of Nrf-2 and HO-1. Therefore, we concluded that CADM5 (CWR:ALL:DO = 2:1:1) effectively improves vascular health by regulating the expression of VCAM-1.
Keywords
Cynanchum wilfordii Radix; Arctium lappa L; Dioscorea opposite; VCAM-1; HASMCs;
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