• Molecules and Cells
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• v.28 no.5
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• pp.479-487
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• 2009

Glutathione reductase (GR) is an enzyme that recycles a key cellular antioxidant molecule glutathione (GSH) from its oxidized form (GSSG) thus maintaining cellular redox homeostasis. A recombinant plasmid to overexpress a GR of Brassica rapa subsp. pekinensis (BrGR) in E. coli BL21 (DE3) was constructed using an expression vector pKM260. Expression of the introduced gene was confirmed by semi-quantitative RT-PCR, immunoblotting and enzyme assays. Purification of the BrGR protein was performed by IMAC method and indicated that the BrGR was a dimmer. The BrGR required NADPH as a cofactor and specific activity was approximately 458 U. The BrGR-expressing E. coli cells showed increased GR activity and tolerance to $H_2O_2$, menadione, and heavy metal ($CdCl_2$, $ZnCl_2$ and $AlCl_2$)-mediated growth inhibition. The ectopic expression of BrGR provoked the co-regulation of a variety of antioxidant enzymes including catalase, superoxide dismutase, glutathione peroxidase, and glucose-6-phosphate dehydrogenase. Consequently, the transformed cells showed decreased hydroperoxide levels when exposed to stressful conditions. A proteomic analysis demonstrated the higher level of induction of proteins involved in glycolysis, detoxification/oxidative stress response, protein folding, transport/binding proteins, cell envelope/porins, and protein translation and modification when exposed to $H_2O_2$ stress. Taken together, these results indicate that the plant GR protein is functional in a cooperative way in the E. coli system to protect cells against oxidative stress.

• Science of Emotion and Sensibility
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• v.20 no.2
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• pp.137-148
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• 2017

The present study examined the effect of stimulus contrast and color on detection of a target embedded in streams of letters. In Experiment 1, each trial displayed four rapid serial visual presentation (RSVP) streams of letters (i.e., multi-RSVP), and each stream occupied one of four different locations. Each frame in the RSVP stream had four white distractors at the locations except one frame where a dim grey target was displayed at a location with three white distractors at the remaining locations. In the low-visibility target condition, the target's grey color was slightly darker than the background grey whereas much dimmer in the high-visibility condition. Participants were asked to report presence of a predesignated target as quickly and accurately as possible upon its detection in each trial, and their target detection turned out more accurate and quicker in the high-visibility than the low-visibility condition. In Experiment 2, the same RSVP displays and task as Experiment were used, but the grey target letters in the high-visibility condition were replaced with those of distinct chromatic colors. Participants detected target presence more accurately in the high-visibility condition, but the reaction time did not differ between the visibility conditions. The results indicate that higher stimulus contrast as well as distinct color can improve perception of a target stimulus displayed among visually-demanding background, but also suggest that stimulus contrast may play a more substantial role for such perceptual improvement.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.19 no.6
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• pp.1-8
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• 2005

The quantity of light available for a space can be translated in term of the amount of energy savings through a process of a building energy simulation. To get significant energy savings in general illumination, the electric lighting system must be incorporated with a daylight - activated dimmer control. A prototype configuration of an once interior has been established and the integration between the building envelope and lighting and HVAC systems is evaluated based on computer modeling of a lighting control facility. First of all, an energy-efficient luminaire system is designed and the lighting analysis program, Lumen-Micro 2000 predicts the optimal layout of a conventional fluorescent lighting future to meet the designed lighting level and calculates unit power density, which translates the demanded met of electric lighting energy. A dimming control system integrated with the contribution of daylighting has been applied to the operating of the artificial lighting. Annual cooling load due to lighting and the projecting saving amount of cooling load due to daylighting under overcast diffuse sky m evaluated by computer software ENER-Win. In brief, the results from building energy simulation with measured daylight illumination levels and the performance of lighting control system indicate that daylighting can save over 70 percent of the required energy for general illumination in the perimeter zones through the year A 25[%] of electric energy for cooling and almost all off heating energy may be saved by dimming and turning off the luminaires in the perimeter zones.

• Korean Journal of Cognitive Science
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• v.12 no.4
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• pp.49-56
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• 2001

When multiple features are presented in the image the computational models for stereopsis select the most activated matches through the excitatory and inhibitory interactions among all possible matches. Using the random-dot stereogram with two surfaces. we investigate whether human binocular mechanism selects the most activated matches. The dots consisting a surface lying in a fixation plane were selected randomly while each of the dots consisting the other surface was paired with each of the original dots in the following manner. After finding the position of each dots in the original random pattern we placed an additional dot to the left and to the right of the original position in each of the left and right image of a stereogram respectively. The luminance of additional dots was varied while that of the original random dots was fixed so that the hypothetical matches presumably could be activated differently. Across the luminance condition the depth of each surface was measured to examine whether matches to be selected were changed depending on the activation level of possible matches. When the luminance of two patterns was within 30% of one another observers perceived an opaque surface. Beyond this value two transparent surfaces were seen with the magnitude of perceived depth varying with relative luminance of two patterns. When original pattern was brighter one additional surface was perceived at the depth corresponding to the disparity of original pattern. When original dot was dimmer. however the depth of an additional surface corresponded to the disparity of newly introduced pattern. These results suggest that there are dynamic interactions within the matching process whereby highly activated matches inhibit weaker one.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.16 no.1
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• pp.199-207
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• 2016

In this paper, we propose efficient smart indoor emotional lighting control system based on android platform using the biological signal. The proposed smart indoor smart emotional lighting control system were configured as the biological signal measurement device and removable smart wall pad, lighting driver, luminaire. The control system was extracts the emotional language by measured the biological signal, and it was transmitted a control signal to each lighting driver using a bluetooth in the wall pad. The lighting driver were designed to control the lighting device through an expansion board by collected control signal and the illuminance information the surrounding. In this case, the wall pad can be selecting of manual control and the bio signal mode by that indoor emotional lighting control algorithms, and it was implemented the control program that possible to partial control by selecting the wanted light. Experiment results of the proposed smart indoor emotional lighting control system, it were possible to the optional control about the luminaire of required area, and the manual control by to adjustable of color temperature with that the efficiently adjustable of lighting by to biological signal and emotional language. Therefore, were possible to effective control for improvement of concentration and business capability of indoor space business conduct by controlling the color and brightness that is appropriate for your situation. And, was reduced power consumption and dimmer voltage, lighting-current than the existing-emotional lighting control system.

• Korean Journal of Poultry Science
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• v.50 no.4
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• pp.261-266
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• 2023

The skeletal muscles of livestock play a crucial role as protein sources for humans, and the consumption of poultry meat is steadily increasing worldwide. Numerous genes, including myogenic regulatory factors, are involved in myogenesis, and precise regulation of them is essential. In this study, genes specifically expressed in muscles were selected, and their promoters were cloned and analyzed. The analysis of gene expression in various tissues of animals revealed that many genes exhibited specific expression patterns in skeletal muscles, with TNNT3, TNNC2, and MYF6 genes showing similar patterns in poultry. The promoter regions of three genes were amplified by polymerase chain reaction to sizes of 1.2 kb, 1.03 kb, and 1.43 kb, respectively. These fragments were then inserted at the front of the enhanced green fluorescent protein gene in vectors. It was confirmed that the sequences of three promoters closely matched the chicken genome sequences. Upon introducing vectors with each promoter into QM7 quail muscle cells, all three promoters successfully induced the expression of the green fluorescent protein. The brightness of the green fluorescence in each promoter was approximately seven times dimmer compared to the control, CMV-IE promoter. It is predicted that more than 230 transcription factors can bind to each promoter, especially various transcription factors expressed in muscles, including myogenic regulatory factors such as MYF5, MYOD, and MYOG. These promoters can be valuable for studying gene expression in poultry muscle cells, and further research is needed to precisely investigate the regulatory region of gene expression in promoters.