• Journal of Animal Environmental Science
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• v.7 no.2
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• pp.119-126
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• 2001

For the odor test, it was chosen the conventional spreader, the hose spreader, and its combination with disk harrow and spring harrow as a slurry spreader. By the odor tester (Kalmor-$\sum$) with dairy slurry, the conventional spreader has the average $\sum$ value of 270, which means that one fells substantial odor and torment. In the cases of hose spreader and its combination with disk harrow, $\sum$ value was 217 and 182 respectively, which means a normal person does not smell any odor. Spreading the swine slurry with a conventional spreader shows $\sum$ value of 440, which means one feels unpleasant. The hose spreader shows $\sum$ value of 258, which lies in the limit one feels some odor and torment. Its combination with disk harrow has $\sum$ value 184, which means that a normal person can not smell any more. For the conventional spreader, the odor intensity indices from air dilution sensual test and 3 point odor bag as a sensual test were 66.9 and 35.4 respectively. On the other hand, the indices were 9.65 and 11.10 by hose spreader and its combination with disk harrow, which were lower than that of the regulation for industry. Therefore, the developed hose spreader showed an excellent effect on decreasing odor. The correlation between the results from odor tester and 3 point odor bag has shown 0.997 by swine slurry.

• Economic and Environmental Geology
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• v.41 no.4
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• pp.383-392
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• 2008

This study aims to evaluate the mechanical-chemical stability of used coal ash blocks, for improving fishing grounds. The surface of weathered ash blocks in seawater showed a decrease in the Ca and an increase in the Mg contents, compared to that of fresh blocks. This result reflects the substitution of Ca by Mg in seawater. The compressive strengths of ash blocks submerged into seawater during 12 months ranged from 235.23 to $447.43\;kgf/cm^2$; this is higher than the standard strength of wave-absorbing blocks($180\;kgf/cm^2)$ that are used for harbor construction. In addition, the compressive strength of ash blocks tends to increase with increasing installation time in seawater. The result of leaching experiments on coal ash blocks by Korean Standard Leaching Test(KSLT) method showed that leached concentrations of most metals except Cr(that leached up to 50 ppb, approaching standard concentration) do not exceed the seawater quality standards. A long-term(112 days) heavy metal leaching test to analyze seawater without mixing-dilution also showed that the concentrations of leached heavy metals, except for Cu, under anaerobic conditions do not exceed the seawater quality standards. Accordingly, the use of coal ash blocks in marine environments appears to be safe from chemical and mechanical factors that decrease the efficiency of concrete. Also, leaching concentration of Cu seems to be stable by decrease of leaching concentration due to dilution of seawater.

• Journal of the Korea Society of Computer and Information
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• v.21 no.5
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• pp.135-139
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• 2016

Scrophularia ningpoensis hemsl has been traditionally used in China and Vietnam for treatment of bacteria, atopy, pimple, tonsillitis, angina and encephalitis for a long time. The main objectives of this study were to evaluate the antibacterial activity of the Scrophularia ningpoensis hemsl extract on biofilm formation of Klebsiella pneumoniae. Antibacterial activity was conducted using disc diffusion assay and minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) were determined using the broth micro dilution method in accordance to Clinical and Laboratory Standards Institute guidelines(CLSI). Furthermore, cytotoxicity on L929 were assessed using animal cell culture for the proliferation test(MTT cell assay) and the biofilm forming capacity of the K. pneumoniae were determined using the colony forming unit (CFU) assay. The extract exhibited considerable antibacterial activity. K. pneumoniae was susceptible to the extract with the MIC and MBC of 0.1875 and $1.5mg/m{\ell}$ respectively. Cytoxicity test in L929 showed no sign of toxicity at the concentration of $0.75mg/m{\ell}$ and at the same concentration the extract caused inhibition of bacterial biofilm formation. The extract of Scrophularia ningpoensis hemsl possesses an in vitro antibacterial antibiofilm activities against K. pneumoniae, with no sign of cytoxicity on L929.

• Korean Journal Plant Pathology
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• v.11 no.4
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• pp.361-366
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• 1995

A virus was isolated from petunia (Petunia hybrida Vilm.) plants showing chlorotic ring spots on the leaves and color breaking on the flowers, and was identified as petunia asteroid mosaic virus (PAMV). Identification of the PAMV was established by host range test, electron microscopy, serological reaction, and physical properties of the virus. In the host range test, Nicotiana glutinosa, N. rustica, N. clevelandii, P. hybrida, Gomphrena globosa, and Chenopodium amaranticolor were systemically infected with the virus. The virus produced local lesions on inoculated leaves of N. tabacum‘Samsun’, N. tabacum‘Xanthi nc’, Datura stramonium, Vigna unguiculata‘White eye’, C. quinoa, Capsicum annuum, Vicia faba, and Lycopersicon esculentum‘Rutgers’. However, Cucurbita sativus and C. moschata did not show any symptoms. PAMV particles were isometric with 30 nm in diameter. The crude sap from G. globosa infected with the virus reacted positively with antiserum to tomato bushy stunt virus (TBSV) in agar gel double diffusion test. Thermal inactivation point of the virus was 8$0^{\circ}C$ and the virus retained its infectivity at the dilution of 10-4. Longevity in vitro of the virus was estimated longer than 35 days.

• Natural Product Sciences
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• v.19 no.4
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• pp.297-302
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• 2013

The current study was conducted to evaluate the antibacterial and antioxidant activities of the essential oil fraction from the roots of Angelica tenuissima Nakai and its main components. We extracted the essential oil fraction from the roots of A. tenuissima using steam distillation and isolated its main components. Their antibacterial activities were determined by broth dilution test against food-borne pathogenic bacteria. Antioxidant activities were evaluated by DPPH-scavenging assay and reducing-power test. Also tested was their ability to inhibit the growth of two gastrointestinal cancer cell lines, Caco-2 and MKN-45. The A. tenuissima oil fraction and its main components, ligustilide and butylidene phthalide exhibited marked inhibitory effects against most of the tested antibiotic-susceptible and antibiotic-resistant bacterial strains with minimum inhibiting concentrations (MICs) from $0.21{\pm}0.08$ to $3.60{\pm}0.89mg/ml$. They also showed growth-inhibiting activity against Caco-2 and MKN-45 cells. The oil fraction showed significant antioxidant activities in DPPH radical scavenging assay and reducing-power test. Taken together, A. tenuissima essential oil could be used as a safe additive for preventing food contamination by pathogenic bacteria. Additionally, its antioxidative activity and the ability to inhibit gastrointestinal carcinoma cell lines could increase its value for functional foods and prevention of cancer.

• Korean Journal of Animal Reproduction
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• v.14 no.2
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• pp.107-114
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• 1990

These experiments were undertaken as a basic study to develop immunocontraceptive vaccine and to understand the role of zona pellucidae in early fertilization process by identifying the monoclonal and polyclonal antibody to porcine zona pellucidae and polyclonal antibody to mouse zona pellucidae by indirect ELISA and indirect immunofluorescence test. The results obtained in these experiments were summarized as follows : 1. The titer of the antibodies to zona was determined by indirect ELISA using solubilized porcine zona coated plates. Both monoclonal and rabbit polyclonal antibodies showed very high titers ; O.D at 1 : 12,800 dilution of antibodies was still significantly higher than that of non-immunized control serum. Rabbit anti-mouse zona pellucidae sera also reacted with porcine zona pellucidae. 2. By indirect immunofluorescence test strong fluorescences were observed on the egg treated with homologous and heterologous rabbit polyclonal antibodies and FITC lablled 2nd antibodies and found to crossreact strongly with the eggs from the pig and mouse. While weaken fluorescences were observed on the eggs treated with monoclonal antibodies.

• Journal of Mechanical Science and Technology
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• v.15 no.10
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• pp.1442-1450
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• 2001

The EGR system has been widely used to reduce nitrogen oxides (NO$\_$x/) emission, to improve fuel economy and suppress knock by using the characteristics of charge dilution. However, as the EGR rate at a given engine operating condition increases, the combustion instability increases. The combustion instability increases cyclic variations resulting in the deterioration of engine performance and emissions. Therefore, the optimum EGR rate should be carefully determined in order to obtain the better engine performance and emissions. An experimental study has been performed to investigate the effects of EGR on combustion stability, engine performance,70x and the other exhaust emissions from 1.5 liter gasoline engine. Operating conditions are selected from the test result of the high speed and high acceleration region of SFTP mode which generates more NO$\_$x/ and needs higher engine speed compared to FTP-75 (Federal Test Procedure) mode. Engine power, fuel consumption and exhaust emissions are measured with various EGR rate. Combustion stability is analyzed by examining the variation of indicated mean effective pressure (COV$\_$imep/) and the timings of maximum pressure (P$\_$max/) location using pressure sensor. Engine performance is analyzed by investigating engine power and maximum cylinder pressure and brake specific fuel consumption (BSFC)

• 대한예방의학회:학술대회논문집
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• 1994.02b
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• pp.164-168
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• 1994

Single serologic tests may occasionally influence clinicians in making diagnoses. The antistreptolysin O (ASO) test is a frequently used tool for detecting recent Streptococcus pyogenes infection and is helpful in the diagnosis of diseases like rheumatic fever. Using data from a 1989 prospective study of 600 healthy male military recruits, in which 43% experienced S. pyogenes upper respiratory tract infection (2-dilution rise in ASO), this report compared two methods of interpreting a single ASO titer. Using the 'upper limit of normal' (80 percentile) method, recruits with an ASO titer of greater than 400 showed evidence of recent S. pyogenes infection. This method had a sensitivity and specificity of only 65.9 and 81.9% respectively. In contrast to the 'yes-no'. dichotomy of the 'upper limit of normal' method. the likelihood ratio method statistics were ASO value specific, more consistent with clinical judgment, and better emphasized the caution clinicians must use in interpreting a single ASO test.

• Korean Journal of Veterinary Research
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• v.42 no.1
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• pp.73-80
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• 2002

An enzyme-linked immunosorbent assay (ELISA) using purified hemagglutinin of swine influenza virus (H1N1) as antigen was developed for detection of antibody to avian influenza virus (AIV). The sensitivity and specificity of a developed and commercial available ELISA kits were compared with those of agar gel precipitation (AGP) test and hemagglutination inhibition (HI) test using sera collected from chickens under condition of field exposure. The concentration of antigen, serum dilution and concentration of enzyme-conjugated secondary antibody in developed ELISA (S-ELISA) were 0.5ug/100ul, 1:200 and 0.03ug/100ul, respectively. The correlation coefficients between S-ELISA and commercial ELISA and HI titers were 0.419 and 0.533, respectively. A significant correlation (p < 0.01) was not found between HI and ELISA titers. The S-ELISA was found to be as more sensitive and specific than the AGP test, showing 86.8% sensitivity and 85.3% specificity. It is suggested that the ELISA using the SIV as antigen may be useful method as an investigating tool for AIV serological surveillance.

• Proceedings of the Korean Nuclear Society Conference
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• 1996.05a
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• pp.413-418
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• 1996

The main purpose of this paper is to develop the modified LTC code for accurate analysis of the boron concentration behavior of all components in the Nuclear Steam Supply System (NSSS). This is achieved by adapting a multi-cell mad to the existing Long Term Cooling (LTC) code. To verify the modified LTC, the simulated results were compared with the actual test results measured during YGN 4 initial criticality test. It was shown that the simulated results of this modified LTC were in good agreement with the actual test results. Also, the boron concentration behavior analysis were performed using the modified LTC code for both direct and indirect dilution/boration nude using YGN 3,4 design data. This modified LTC code can provide a valuable information in predicting boron concentration behavior during power maneuvering such as startup operation, shutdown operation and load follow operation. It is expected that the modified LTC can be applied to both on-line and off-line mode using Plant Computer System(PCS).