• Title/Summary/Keyword: Dilution rate

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Biofuel Production by Immobilized Living Cells - Hydrogen Production by Photosynthetic Bacteria - (고정화 미생물에 의한 에너지 생산 - 광합성 박테리아에 의한 수소 생산 -)

  • 조영일;선용호
    • Microbiology and Biotechnology Letters
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    • v.13 no.3
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    • pp.303-309
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    • 1985
  • Continuous production of hydrogen by Ca alginate-immobilized photosynthetic bacteria was studied in a packed-bed bioreactor. The dilution rate and input concentration of carbonaces substrate were selected as operating parameters. To choose the strain for immobilization, hydrogen productivities of Rhodopseudomonas caposulata 10006 and Rhodospirillum rubrum KS-301 were compared through preliminary batch cultures of their free cells: the former was found to show better hydrogen productivity in spite of its lower specific growth rate. For the continuous production of hydrogen by immobilized R capsulata, the optimum dilution rate was about 0.84 h$^{-1}$ . The Immobilized tells gave better hydrogen yield and conversion efficiency than free ones. And a kinetic parameter K'$_{m}$ was determined for the packed-bed bioreactor, being practically constant for a specific range of dilution rates.s.

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Treatment of Acid dye Using Microbial Immobilization (미생물 고정화를 이용한 산성염료의 처리)

  • 김정목;조무환;양용운
    • Textile Coloration and Finishing
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    • v.11 no.2
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    • pp.19-26
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    • 1999
  • Strains degrading and decolorizing acid dyes, Nylosan red E-BL 150%. were isolated from natural system, was named as ARK3. The optimal culture conditions of temperature and pH were $35^\circ{C}$, 7.0, respectively. Growth rate of cells in conditions of aerobic shaking more than standing culture conspicuously increased, and optical density of those to strain ARK3 were found as 1.38 and 0.25 after 42 hrs. Decolorization efficiency in batch culture which used as immobilization media to natural zeolite was 15% after 6 hrs, while suspension culture was 5%, also its of immobilization and suspension culture were 90% and 85% after 48 hrs, respectively. Decolorization efficiency of air-lift bioreactor was more than 90% to a dilution rate of $0.038hr^{-1}$, but that was decreased as 70%, when the dilution rate was $0.05hr^{-1}$. Even though at maximum dilution rate of this study, there was not appeared "wash out" phenomienon of biomass. Decolorization efficiency was 97.7% at a dilution rate of $0.025hr^{-1}$, when influent dye concentration was $100mg/\ell$. But if influent dye concentration increased as $150mg/\ell$, even though MLVSS increased, that of treatment water decreased as 93%. Also, when influent dye concentration increased as $200mg/\ell$ and $300mg/\ell$, decolorization efficiencies of treatment water abruptly decreased as 85% and 63%, respectively. Decolorization efficiency was more than 92% to the limit volumetric loading rate of $3.75mg/\ell\cdot{hr}$hr, without regard to variation of influent dye concentration or hydraulic retention time. if volumetric loading rate was more than $3.80mg/\ell\cdot{hr}$, at same condition, decolorization efficiency was lower decrease of retention time than increase of influent dye concentration.entration.

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Analysis of Contrast Medium Dilution Rate for changes in Tube Current and SOD, which are Parameters of Lower Limb Angiography Examination (하지 혈관조영검사 시 매개변수인 관전류와 SOD에 변화에 대한 조영제 희석률 분석)

  • Kong, Chang gi;Han, Jae Bok
    • Journal of the Korean Society of Radiology
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    • v.14 no.5
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    • pp.603-612
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    • 2020
  • This study has a purpose to look into the effect of the relationship between the Tube current (mA) and SOD(Source to Object Distance), which is a parameter of lower limb angiography examination, and the dilution rate of the contrast medium concentration (300, 320, 350) on the image. To that end, using 3 mm vessel model water phantom, a vessel model custom made in the size of peripheral vessel diameter, this study measured relationships between change of parameters, such as tube current (mA), SOD and varying concentrations (300, 320, 350) of contrast medium dilution into SNR and CNR values while analyzing the coefficients of variance(cv<10). The software used to measure SNR and CNR values was Image J 1.50i from NIH (National Institutes of Health, USA). MPV (mean pixel value) and SD (standard deviation) were used after verifying numerically the image signal for region of interest (ROI) and background on phantom from the DICOM (digital imaging and communications in medicine) 3.0 file transmitted to PACS. As to contrast medium dilution by the change of tube current, when 146 mA and 102 mA were compared, For both SNR and CNR, the coefficient of variation value was less than 10 until the section of CM: N/S dilution (100% ~ 30% : 70%) but CM: N/S dilution rate (20%: 80% ~ 10% : 90%) the coefficient of variation was 10 or more. As to contrast medium dilution by concentration for SOD change, when SOD's (32.5 cm and 22.5 cm) were compared,For both SNR and CNR, the coefficient of variation value was less than 10 until the section of CM: N/S dilution (100% ~ 30% : 70%) but CM: N/S dilution rate (20%: 80% ~ 10% : 90%) the coefficient of variation was 10 or more. As to contrast medium dilution by concentration for SOD change, when SOD's (32.5 cm and 12.5 cm) were compared,For both SNR and CNR, the coefficient of variation value was less than 10 until the section of CM: N/S dilution (100% ~ 30% : 70%) but CM: N/S dilution rate (20%: 80% ~ 10% : 90%) the coefficient of variation was 10 or more. As a result, set a low tube current value in other tests or procedures including peripheral angiography of the lower extremities in the intervention, and make the table as close as possible to the image receiver, and adjust the contrast agent concentration (300) to CM: N/S dilution (30%: 70%). ) Is suggested as the most efficient way to obtain images with an appropriate concentration while simultaneously reducing the burden on the kidney and the burden on exposure.

Evaluation of Roadmap Image Quality by Parameter Change in Angiography (혈관조영검사에서 매개변수 변화에 따른 Roadmap 영상의 화질평가)

  • Kong, Chang gi;Song, Jong Nam;Han, Jae Bok
    • Journal of the Korean Society of Radiology
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    • v.14 no.1
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    • pp.53-60
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    • 2020
  • The purpose of this study is to identify factors affecting picture quality in Roadmap images, which were studied by varying the dilution rate, collimation field and flow rate of contrast medium. For a quantitative evaluation of the quality of the picture, a 3mm vessel model Water Phantom was self-produced using acrylic, a roadmap image was acquired with a self-produced vascular model Water Phantom, and the SNR(Signal to Noise Ratio) and CNR (Contrast to Noise Ratio) were analyzed. CM:N/S In the study on the change of dilution rate, CM:N/S dilution rate changed to (100%~10%:100%), and the measurement of the roadmap image taken using the vascular model Water Phantom showed that the measurement value of SNR gradually decreased as the N/S dilution rate was increased, and the measurement of CNR was gradually reduced. It was confirmed that the higher the dilution rate of CM:N/S, the lower the SNR and CNR, and also significant image can be obtained at the dilution rate of CM:N/S (100%~70:30%). The study showed the value of SNR and CNR in Roadmap image was increased as the Collimation Field was narrowed to the center of the vascular phantom; the Collimation Field was narrowed to the center of the vessel model by 2cm intervals to 0cm through 12cm. To verify the relationship with Roadmap image and Flow Rate, volume of the autoinjector was kept constant at 15 and the flow rate was gradually increased 1, 2, 3, 4, 5, 6, 7, 8, 9, 10. The value of SNR and CNR of images taken by using water Phantom gradually decreased as the Flow Rate increased, but at Flow Rate 9 and 10, the SNR and CNR value was increase. It was not possible to confirm the relationship with SNR and CNR by ROI mean value and Background mean value. It is considered that further study is needed to evaluate the correlation about Roadmap image and Flow Rate. In conclusion, as the dilution rate of N/S in contrast medium was increased, the value of SNR and CNR was decreased. The narrower the Collimation Field, the higher image quality by increasing value of SNR and CNR. However, it is not confirmed the relationship Roadmap image and Flow Rate. It is considered that appropriate contrast medium concentration to minimize the effects of kidney and proper Collimation Field to improve contrast of image and reduce exposure X-ray during procedure is needed.

Verification of dilution ratio of the newly developed ejector-porous tube diluter for measurement of fine dust in coal-fired power plant stack (화력발전소 굴뚝 미세먼지 측정을 위해 개발한 이젝터-다공튜브 희석장치의 희석비 검증)

  • Shin, Dongho;Kim, Young-Hoon;Hong, Keejung;Kim, Hak-Joon;Kim, Yong-Jin;Han, Bangwoo;Lee, Ga-Young;Chun, Sung-Nam;Hwang, Jungho
    • Particle and aerosol research
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    • v.15 no.3
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    • pp.105-113
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    • 2019
  • The exhaust emissions from coal-fired power plants have received much attention because coal-fired power plants are the one of the largest sources of particulate matter (PM) emissions in South Korea. To measure the PM10 and PM2.5, we developed the novel diluter which is comprised of ejector and porous tube in series. The dilution ratio must be defined to calculate particle concentrations of the sampled air as well as to probe match for the isokinetic sampling. For this reason, we verified the dilution ratio of the developed diluter by the flow rate, numerical solution, gas concentration and particle concentration. The ejector-supplied flow rates were 10-50 L/min and the porous tube-supplied flow rates were 30, 50 L/min in this study. All methods above showed similar dilution ratios to each other within 10 % error rate. The dilution ratio was confirmed by comparing mass concentrations before and after the dilution process.

Wear performance of Plasma Transferred Arc deposited layers

  • Yoon, Byoung-Hyun;Kim, Hyung-Jun;Lee, Chang-Hee
    • Proceedings of the KWS Conference
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    • 2001.05a
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    • pp.245-247
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    • 2001
  • In this study, the effects of dilution on the wear behavior of PTAW (Plasma Transferred Arc Welding) Inconel 625, Inconel 718 and Stellite 6 overlays on Nimonic 80A were investigated. Inorder to evaluate the wear performance, two-body and three-body abrasive wear test, and dry sliding wear test were performed. According to wear tests, the wear rate of deposit with dilution 30% was higher than that of dilution 10% by 10%, and it was also found the plastic deformation near worn surface.

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Evaluation of antibacterial activity against Candida albicans according to the dosage of various denture cleansers

  • Bae, Cha-Hwan;Lim, Yun-Kyong;Kook, Joong-Ki;Son, Mee-Kyoung;Heo, Yu-Ri
    • The Journal of Advanced Prosthodontics
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    • v.13 no.2
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    • pp.100-106
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    • 2021
  • PURPOSE. The purpose of this study is to compare the antibacterial activity of currently purchasable denture cleansers against Candida albicans. Materials and methods: This study used tablet-type denture cleansers, PolidentⓇ, CoolingdentⓇ and FittydentⓇ, along with liquid denture cleansers, HexamedineⓇ, ListerineⓇ and Apple vinegarⓇ. The antibacterial activities of denture cleansers were evaluated based on the number of C. albicans and concentrations of the denture cleansers. Results. In the 0.5 × 106 cfu/㎖ culture medium, the C. albicans' death rate of PolidentⓇ was significantly lower than those of FittydentⓇ, HexamedineⓇ, ListerineⓇ, and Apple vinegarⓇ(P<.05). In the 0.5 × 107 cfu/, the C. albicans' death rates of PolidentⓇ and CoolingdentⓇ were significantly lower than those of FittydentⓇ, HexamedineⓇ, ListerineⓇ and Apple vinegarⓇ(P<.05). The C. albicans' death rates of PolidentⓇ and CoolingdentⓇ were significantly decreased at 0.02 g and 0.01 g. The C. albicans' death rate of FittydentⓇ was significantly decreased at 0.005 g (P<.05). The C. albicans' death rate of HexamedineⓇ was significantly decreased at 1/16 dilution. The C. albicans' death rate of ListerineⓇ was decreased at 1/8 dilution, and the antibacterial activity of Apple vinegarⓇ was decreased at 1/4 dilution (P<.05). Conclusion. As the number of C. albicans increased, the antibacterial activities of the denture cleansers decrease. In the tablet-type denture cleanser, all denture cleansers showed 100% C. albicans' death rate when used at a dose of 1 tablet. One denture cleanser showed the same antibacterial effect with only 1/3 of a tablet. In the liquid type denture cleanser, the level of dilution required was different for each denture cleanser.

Development of continuous process for the production of Exo-biopolymer with Cordyceps militaris

  • Kim, Kyoung-Ju;Lee, Jung-Heon
    • 한국생물공학회:학술대회논문집
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    • 2003.10a
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    • pp.243-245
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    • 2003
  • The continuous fermentation process for exe-biopolymer production with Cordyceps militaris has been developed in this research. With the change of dilution rate, biopolymer production has been changed and the maximal biopolymer production has been achieved when the dilution rate was 0.0225/hr. The maximum mycelium concentration and biopolymer concentration were 8 g/L and 4.2 g/L, respectively.

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Production of Beijerinckia indica HS-2001 in Fed-batch and continuous culture.

  • Yang, Jae-Gyun;Seo, Hyeong-Pil;Sin, Myeong-Gyo;Lee, Jin-U
    • 한국생물공학회:학술대회논문집
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    • 2003.04a
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    • pp.245-248
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    • 2003
  • As a result of broth substitutions when each culture-mediums were difference, whole culture-medium was found to be best feeding solution for production of PS-7 by B. indica. Maximal production of PS-7 was 1$10.0\;g/{\ell}$ and its conversion rate from 2% (w/v) glucose to PS-7 was 50%. After 48 hr, 50%(v/v) medium of working volume began to substitute in 7L jar fermenter. Production of PS-7 increased after 48hr, recovered productivity of PS-7. Following this preliminary culture, the resultant culture was subjected to continuous flow conditions controlled that the dilution rate were $0.01\;{\sim}\;0.04\;h^{-1}$. Production of PS-7 increased at dilution rate $0.0100\;h^{-1}$ whereas productivity of PS-7 decreased gradually in dilution rate $0.0200\;{\sim}\;0.0400\;h^{-1}$. Maximal production of PS-7 was $10.0\;g/{\ell}$ in continuous culture.

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Estimation and Sensitivity Analysis of Kinetic Parameters for Plasmid Stability in Continuous Culture of a Recombinant Escherichia coli Harboring trp-operon Plasmid

  • NAM, SOO WAN;BYUNG KWAN KIM;JUNG HOE KIM
    • Journal of Microbiology and Biotechnology
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    • v.4 no.1
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    • pp.13-19
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    • 1994
  • A model equation to describe the plasmid instability in recombinant Escherichia coli fermentation is proposed. The equation allows one to estimate easily the two model parameters; (1) the difference in the specific growth rates between plasmid-free cells and plasmid-harboring cells ($\delta$), and (2) the probability of plasmid loss by plasmid-harboring cells ($\rho$). The estimated values of $\delta and \rho$ were in the range of 0.02-0.07 and $10^{-3}-10^{-5}$, respectively, and were strongly dependent on the dilution rate. As another parameter, the ratio of specific growth rates of plasmid-free cells and plasmid-harboring cells ($\alha$) was calculated and the result showed the highest value of 1.28 at the lowest dilution rate of 0.075 $hr^{-l}$, examined in this work. By the sensitivity analyses on the estimates of $\delta and \rho$, it was found that the growth rate difference ($\delta$) affected the plasmid instability more seriously than the probability of plasmid loss ($\rho$). Furthermore, the profound instability of plasmid at low dilution rate could be explained by the high values of $\alpha and \rho$.

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