• Asian-Australasian Journal of Animal Sciences
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• v.29 no.8
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• pp.1152-1158
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• 2016

Morphological and physiological alterations occur in the digestive system of weanling piglets, compromising the performance in subsequent phases. This experiment aimed at verifying the influence of glutamine, glutamate and nucleotides on the carbon turnover in the pancreas and liver of piglets weaned at 21 days of age. Four diets were evaluated: glutamine, glutamic acid or nucleotides-free diet (CD); containing 1% glutamine (GD); containing 1% glutamic acid (GAD) and containing 1% nucleotides (ND). One hundred and twenty-three piglets were utilized with three pigs slaughtered at day zero (weaning day) and three at each one of the experimental days (1, 2, 4, 5, 7, 9, 13, 20, 27, and 49 post-weaning), in order to collect organ samples, which were analyzed for the ${\delta}^{13}C$ isotopic composition and compared by means of time. No differences were found (p>0.05) among treatments for the turnover of the $^{13}C$ in the pancreas ($T_{50%}$ = 13.91, 14.37, 11.07, and 9.34 days; $T_{95%}$ = 46.22, 47.73, 36.79, and 31.04 days for CD, GD, GAD, and ND, respectively). In the liver, the ND presented accelerated values of carbon turnover ($T_{50%}=7.36$ and $T_{95%}=24.47days$) in relation to the values obtained for the GD ($T_{50%}=10.15$ and $T_{95%}=33.74days$). However, the values obtained for the CD ($T_{50%}=9.12$ and $T_{95%}=30.31days$) and GAD ($T_{50%}=7.83$ and $T_{95%}=26.03days$) had no differences (p>0.05) among other diets. The technique of $^{13}C$ isotopic dilution demonstrated trophic action of nucleotides in the liver.

• Economic and Environmental Geology
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• v.38 no.2 s.171
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• pp.135-142
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• 2005

In order to investigate the contamination level and seasonal variation of heavy metals and evaluate the bioavailability of toxic elements, environmental geochemical survey was undertaken at the Dogok Au-Ag-Cu mine area. The main pollution sources in the area were suggested as tailings, mine waste materials and mine water. Elevated levels of $140{\cal}mg/{\cal}kg{\;}As,{\;}107{\cal}mg/{\cal}kg{\;}Cd,{\;} 3017{\cal}mg/{\cal}kg{\;}Cu,{\;}12926{\cal}mg/{\cal}kg{\;}Pb,{\;}9094{\cal}mg/{\cal}kg$ Zn(before rainy season) were found in mine tailings. Concentrations of heavy metals in farmland soils exceeded normal level in nature soil (Bowen, 1979). The highest level of heavy metals was found in water samples near the mine tailing dumps regarded as a main pollution source of toxic elements in the area. These concentrations decreased to downstream due to the effect of dilution. From the results of sequential extraction analyses for tailings and soils, non-residual forms of heavy metals were found, which indicate the contamination to be progressing by continuing weathering and oxidation. Cadmium and Zn would be of the highest mobility in all samples. The bioavailability of Cd, Cu, Zn and As using SBET analysis from paddy soils was $53.3{\%},{\;}46.5{\%},{\;}41.0{\%}$ and $37.0\%$, respectively. The farmland soil sample(S3) showed the highest total concentration and bioavailability of heavy metals.

• Journal of Life Science
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• v.25 no.9
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• pp.1036-1042
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• 2015

By using effective sanitizers, early block for transmission of pathogens is the effective way to prevent epidermic outbreaks. Here we developed a chlorine oxide bubbling type of sanitizing tablets and evaluated the disinfectant and sanitization effects. The sanitizers showed 99.999% of sanitization effect for Escherichia coli ATCC 10536 and Staphylococcus aureus ATCC 6538 strains for 5 min±10 sec on 20±1℃ in clean condition by dilution-neutralization method. It reduced more than 5 log₁₀ cfu/ml of a legal permission standard of colony reduction. When a few used socks and underwear soaked for one hour in the 0.1% of the sanitizing tablets, no microorganisms were grown on CHROMagar plates. However, on CHROMagar plates of the no sanitizing tablets treated control, about 6.5×10⁴ cfu/ml of Staphylococcus sp., Klebsiella sp. and Enterococus sp. were grown. Furthermore, the sanitizing tablets killed approximately 1.5x10⁸ cfu/ml of E. coli BL21 in 5 minutes. Therefore, we concluded that the chlorine based bubbling type of sanitizing tablets satisfied the legal standard for the regulation of food and drug safety for disinfectants and sanitizers to pathogens and daily supplies.

• Korean Journal of Veterinary Research
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• v.34 no.3
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• pp.457-463
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• 1994

Insulin-like growth factor-I(IGF-I) plays an important role in the regulation of mammalian and poultry growth. IGF-I has many actions in different tissues, which include metabolic, mitogenic, and differentiative actions. IGF-I induces insulin-like effects - such as increased cell glucose uptake and glycogen sysnthesis, however several physiological actions of IGF-I may not have been identified yet. In order to investigate the effect on growth in broiler chicken treated with exogenous insulin-like growth factor-I, 30 chickens were injected $50{\mu}g$ reconbinant human IGF- I (rhIGF- I ) per kg body weight as experimental group and 30 ckickens saline subcutanously as control, 3 times according to ages from 2 to 35 days. We established radioimmunoassay method by which we can measure chicken IGF- I (cIGF- I ) as in rhIGF- I assay. The results obtained were as follows; 1) The dilution curve showed in parallelism between rhIGF- I and cIGF- I in the Sep-pak $C_{18}$ cartridge plasma extracts. 2) The body weight of broiler chicken were significantly increased at 31 days($1,176.50{\pm}99.79g$) and 35 days($1,252.84{\pm}125.21g$) of age in treatment groups, compared with control group($1,011.88{\pm}40.22g,\;1,111.32{\pm}153.67g$). The liver and kidney weights on 35 days$(35.24{\pm}5.18g,\;11.05{\pm}1.47g)$ were significantly higher in rhIGF- I treated group than control group($30.95{\pm}4.04g,\;10.01{\pm}1.60g$) 3) The plasma concentration of IGF- l and total protein in rhIGF- I treated group were $58.17{\pm}1.69ng/ml$, $3.75{\pm}0.62g/dl$ respectively compared with control group $45.70{\pm}1.64ng/ml$, $2.32{\pm}0.53g/dl$. The results suggest that exogenous rhIGF- I increased total body weight, liver and kidney weights in broiler chicken, and it may increase IGF- I and total protein concentration in serum.

• Journal of Wetlands Research
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• v.11 no.3
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• pp.37-47
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• 2009

This research was conducted at the constructed wetland in Goheung reclaimed land, and water quality components were measured at the 12 points in 15 March 2008 and 10 January 2009, respectively. Temperature, pH, DO, EC and salinity components were measured at the field, and TOC, Cl-, COD, TSS, T-P and TN components were analyzed laboratory. Concentrations of field measured components at inflow points were higher than in constructed wetland. TOC concentration ratio of inflow water to constructed wetland water was higher in January, and Cl concentration ratio of it was higher in March. And, COD concentration ratio of it were 1.37 for March and 1.49 for January, respectively. T-P and T-N concentration ratios of it at inflow points were higher 3 times than in constructed wetland. Constructed wetland attenuated concentration of contaminated components inflow to it. Removal efficiencies of Cl-, T-P and T-N components in inflow water were high at the constructed wetland. removal efficiencies of Cl component were 83% for 1st monitoring and 76% for 2nd monitoring, this removal efficiency be caused by dilution effect of constructed wetland. removal efficiencies of T-P component were 67% for 1st monitoring and 69% for 2nd monitoring, and they of T-N component were 100% for 1st monitoring and 95% for 2nd monitoring. Abnormal removal efficiency of T-N component is caused that nitrogen in inflow water was a little. Removal efficiency of T-P component was higher in January, and T-N component was higher in March. This is caused by environmental difference between growing season and winter.

• Journal of Food Hygiene and Safety
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• v.16 no.3
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• pp.200-205
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• 2001

In this study, occurrence of aflatoxin M$_1$(AEM$_1$) in domestic milk and milk products was determined. The level of AFM$_1$ in market milk (0.047 ppb) was lower than that in raw milk (0.083 pub) but this looks like that is due to dilution in collecting process rather than the effect of sterilization. In the case of nonfat dry milk, level of AFM$_1$appeared high by 0.24 ppb but it is thought to be not different from market milk actually because nonfat dry milk is diluted at intake. In the case of ice cream, finished products were contaminated with AFM$_1$of 0.020 ppd and also have the possibility of the contamination of AFB$_1$due to secondary raw material such as nuts and almond. On the basis of the results of this study and previous studies, Monte-Carlo simulation is conducted to estimate the contamination level of AFM$_1$in domestic market milk. To consider uncertainty and variability fitting procedure was passed through. And we used beta distribution to estimate the prevalence and triangular distribution to estimate the concentration level of AFM$_1$in milk. As a result, the 5%, 50% and 95% points of the distribution of the probability of AFM$_1$contamination level in milk is 0.0214, 0.0946 and 0.1888 ppb, respectively. Also we estimate that AFM$_1$in almost milk was low more than 0.5 ppb that is American acceptable level but 80.4% exceeded far 0.05 ppb that is European standard.

• Journal of the Korean Institute of Gas
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• v.15 no.5
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• pp.25-30
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• 2011

Biogas obtained from the biodegradable organic wastes in an anaerobic digester consists of $CH_4$ and inert gases such as $CO_2$ and $N_2$. Since the composition of biogas varies by anaerobic digester conditions and the origin of wastes, it is necessary to respond to these variations so as to make stable combustion and accomplish high efficiency when it is used as a fuel for power generating SI engines. In this study, efforts have been made to investigate the effect of changes in the calorific values of biogas on the engine performance and exhaust characteristics. The biogas was simulated by supplying of $CH_4$ with $N_2$ dilution of various ratios, and ECM was developed to achieve accurate control of ignition and combustion. The results show that as the $CH_4$ concentration of the biogas decreases, the optimal spark timing is advanced due to the elevated thermal capacity and lowered $O_2$ concentration of the in-cylinder charge. Furthermore, since combustion temperature was reduced by increased inert gas, $NO_x$ emissions decreased, whereas THC emissions increased.

• Microbiology and Biotechnology Letters
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• v.28 no.2
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• pp.117-123
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• 2000

The object of this work is to improve the maintenance of bioluminescence from stored Photobacterium phosphoreum in a view of developing continuous monitoring system for pollutants. The long-term experiments were performed to determine the effect of storage temperature and immobilization on the maintenance of bioluminescence and viability of P. phosphoreum. A naturally luminescent bacterium, P. phosphoreum was starved in 2.5% Nael solution at $20^{\circ}C$, $4^{\circ}C$, -$20^{\circ}C$ and -$70^{\circ}C$ for 30 days. In vivo luminescence was measured by luminometry, and total cell concentrations and concentrations of culturable and viable cells were determined by acridine orange staining, dilution plate counting, and direct viable counting, respectively. The bioluminescence emission from cells stored at 4De was maintained up to 10 days while those with starved cells at other temperature ranges decreased to background level within 3 days. In terms of viability of cells, concentrations of cells stored at $20^{\circ}C$ were rapidly decreased as a result of cell lysis, leading to a drop in culturable and viable counts while cells stored at $4^{\circ}C$ was shown viable but nonculturable state during starvation. With immobilized cells on strontium alginate, the bioluminescence showed higher maintenance than free cells and decreased with count number of nonculturable cells.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.12
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• pp.1340-1346
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• 2005

A water quality modeling study was performed for Chunggye stream during combined sewer overflow(CSO) period, utilizing the diagnostic system for water management in small watershed, CREEK-1(Cyber River for Environment and Economy in Korea). This system integrated geogaphic information system, data base, landscape ecological model(FRAGSTATS), watershed model(SWMM), water quality model (WASP5), and computer graphic. In this study, the watershed model and water quality model were extensively utilized so as to simulate water qualities and flow in Chunggye stream during wet periods. The Chunggye stream watershed was divided into 18 sub-basins in the watershed model and the stream reach into 11 segments in the water quality model. The watershed model was validated against field measurements of BOD, TN, TP, and flow at the downstream location, where the model results showed a reasonable agreement with the field measurements at all parameters. From this study, it was shown that the stream water quality would change along with elapsed time from rainfall start as well as rainfall intensity. The model results indicated that the water quality would significantly upgrade due to the first flush and high sewage ratio of CSO at the beginning of rainfall event, but become degraded along with the runoff increase due to dilution effect.

• The Korean Journal of Pesticide Science
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• v.20 no.1
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• pp.1-6
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• 2016

In order to obtain efficient control effect of the pesticide, it is important to ensure uniform adhesion to the desired plant parts at the right time. Pesticide spray method (application technology) is an important factor affecting the efficacy and crops persistent expression. The aim of this study was to develop an efficient system to investigate the coating weight distribution of citrus leaves due to the difference between the nozzle and spray sprinkler system using dithianon used in citrus scab. Other An, engine type sprayer was used as the control. Speed sprayer and different sprinklers were wsed to way the deposit amounts of dithianon on citrus leaves. The test was conducted at the National Institute of Horticultural Herbal Science Citrus Research Station, located in the circle citrus Jeju Island. In order to examine whether the citrus orchard spray and the evenl on the whole, dithianon (43% flowable 1000-fold dilution) was sprayed, filter paper and leaves were analyzed by the height as top, middle, bottom. Speed sprayer the was most effective on depositing at the middle position, of the leaves. All other sprays the leaces except the dry mist sprinkler were not effective enough to deposit on the back sides. To achieve more deposits on the high position leaves, an improve ment in the nozzle and an efficient power system of sprayer were needed.