• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.3
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• pp.393-398
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• 1998

The effect of fermentation period on the accumulation of 55,600 dalton polypeptide was analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) as a quality parameter of anchovy sauces. Also, proximate compositions, total nitrogen contents, amount of specfic pepited and isoelectric point(pI0 were investigated during fermentation periods. Total nitrogen contents significantly increased until 18 months. Polypeptide of 55,600 and 46,900 dalton on SDS-PAGE and pI 5.2, 5.6 and 6.0 on isoelectric focusing were identified in all the samples. Especially, the amount of 55,600 dalton had no important change during fermentation periods, and it had a high correlation with dilution degree of anchovy sauces diluted with water. The results could be suggest that the amonts of 55,600 dalton polypeptide will be index for quality estimation of commerical anchovy sauces.

• Journal of Microbiology and Biotechnology
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• v.13 no.5
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• pp.759-766
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• 2003

Recombinant Chinese hamster ovary (rCHO) cell lines expressing a high level of human thrombopoietin (hTPO) or its analog, TPO33r, were obtained by transfecting expression vectors into dihydrofolate reductase-deficient (dhfr) CHO cells and subsequent gene amplification in media containing stepwise increments in methotrexate (MTX) level such as 20, 80, and 320 nM. The parental clones with a hTPO expression level $>0.40\;{\mu}g/ml$ (27 out of 1,200 clones) and the parental clones with a TPO33r expression level $>0.20\;{\mu}g/ml$ (36 out of 400 clones) were subjected to 20 nM MTX. The clones that displayed an increased expression level at 20 nM MTX were subjected to stepwise increasing levels of MTX such as 80 and 320 nM. When subjected to 320 nM MTX, most clones did not display an increased expression level, since the detrimental effect of gene amplification on growth reduction outweighed its beneficial effect of specific TPO productivity ($q_{TPO}$) enhancement at 320 nM MTX. Accordingly, the highest producer subclones ($1-434-80^{*}$ for hTPO and $2-3-80^{*}$ for TPO33r), whose $q_{TPO}$ was 2- to 3-fold higher than that of their parental clones selected at 80 nM MTX, were isolated by limiting dilution method and were established as rCHO cel1 lines. The $q_{TPO}$ of $1-434-80^{*}\;and\;2-3-80^{*}\;was\;5.89{\pm}074\;and\;1.02{\pm}0.23\;{\mu}g/10^6$ cells/day, respectively. Southern and Northern blot analyses showed that the enhanced $q_{TPO}$ of established rCHO cell lines resulted mainly from the increased TPO gene copy number and subsequent increased TPO mRNA level. The hTPO and TPO33r produced from the established rCHO cell lines were biologically active in vivo, as demonstrated by their ability to elevate platelet counts in treated mice.

• Journal of Chest Surgery
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• v.49 no.3
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• pp.145-150
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• 2016

Background: Extracorporeal circulation (ECC) can induce alterations in blood viscoelasticity and cause red blood cell (RBC) aggregation. In this study, the authors evaluated the effects of pump flow pulsatility on blood viscoelasticity and RBC aggregation. Methods: Mongrel dogs were randomly assigned to two groups: a nonpulsatile pump group (n=6) or a pulsatile pump group (n=6). After ECC was started at a pump flow rate of 80 mL/kg/min, cardiac fibrillation was induced. Blood sampling was performed before and at 1, 2, and 3 hours after ECC commencement. To eliminate bias induced by hematocrit and plasma, all blood samples were adjusted to a hematocrit of 45% using baseline plasma. Blood viscoelasticity, plasma viscosity, hematocrit, arterial blood gas analysis, central venous $O_2$ saturation, and lactate were measured. Results: The blood viscosity and aggregation index decreased abruptly 1 hour after ECC and then remained low during ECC in both groups, but blood elasticity did not change during ECC. Blood viscosity, blood elasticity, plasma viscosity, and the aggregation index were not significantly different in the groups at any time. Hematocrit decreased abruptly 1 hour after ECC in both groups due to dilution by the priming solution used. Conclusion: After ECC, blood viscoelasticity and RBC aggregation were not different in the pulsatile and nonpulsatile groups in the adult dog model. Furthermore, pulsatile flow did not have a more harmful effect on blood viscoelasticity or RBC aggregation than nonpulsatile flow.

• Korean Journal of Agricultural Science
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• v.32 no.2
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• pp.233-241
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• 2005

The objective of this study was to investigate the effect of odor removal such as $NH_3$, volatile organic acid (VOA) during the pig manure composting using of the Pyroligneous acid liquor (PAL). The odor removal efficiencies were evaluated throughout the dilution rates (${\times}0$, ${\times}100$, ${\times}300$) of PAL. The results of the study are as followings. $NH_3$ content in all treatments (volume 10L) was approximately 8 mg/kg in compost piles on the first day. In the case of control treatment, it was increased by 46 mg/kg within 10 days and was not detected after 25 days. In contrast, those levels in PA-100 and PA-300 treatments were similar values at 38 mg/kg within 6 days and decreased after 15 days. And GC. equipment was used to analyze the volatile organic acids (acetic acid, butyric acid, isobutyric acid, isovaleric aicd, 2,3-methylbutyric aicd, and propionic acid). The concentration of acetic acid and butyric acid were the higher than other VOAs during the initial composting day and sharply declined after 15 days. The VOAs in the control treatment were detected until 40 days, but PA-100 and PA-300 treatments were not measured after 15 days. The PA-100 in all treatments was the most effective treatment for $NH_3$ gas and VOAs reduction during composting. In addition, these studies for the odor reduction in compost manufacture process would have to research on more effective methods for the PAL utilization in agro-biological aspects.

• Research in Plant Disease
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• v.26 no.2
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• pp.61-71
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• 2020

With 32 fungicides, it was examined the inhibitory effects on the mycelial growth of Alternaria dauci KACC42997 causing Alternaria leaf blight of carrot. Showing the results of the agar dilution method, the fungicides belonging to C2, C5, G1, E2, and E3 group were excellent in inhibiting mycelial growth. Protective fungicides belonging to M group, except for iminoctadine tris-albesilate, and pyraclostrobin belonging to C3 group were effective in inhibiting spore germination of pathogens. The fungicides included into C2 group inhibiting succinate dehydrogenase activity and the G1 group inhibiting demethylase activity showed the excellent inhibitory effect on mycelial growth but the inhibitory effect of spore germination was very low. However, fluazinam belonging to C5 group was excellent in inhibiting spore germination as well as mycelial growth. Especially, when 100 ㎍/ml of fluxapyroxad belonging to the C2 group was treated, 47.1% of spore formation was inhibited on the medium. In comparison of the resistance factors of 3 fungicide groups, as G, C, and E group, in populations of A. dauci isolates collected from Gumi, Pyeongchang, and Jeju, resistance factor in the population of Jeju was the lowest. However, two isolates resistant to fludioxonil belonging to E2 group were found in the isolate group of Pyeongchang, and both showed cross-resistance to iprodione and procymidone.

• The Korean Journal of Mycology
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• v.23 no.2 s.73
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• pp.153-160
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• 1995

As part of our search for less toxic antimicrobial agents from natural resources, the antimicrobial activity of Elfvingia applanata $(P_{ers.})\;K_{arst.}$ extract was examined alone and in combination with naringenin. EA, the aqueous extract from the carpophores of E. applanata, was lyophilized and a dark brownish powder was obtained. Antimicrobial activity of EA was tested in vitro against nineteen strains of bacteria and eleven strains of fungi by serial broth dilution method, and expressed by minimal inhibitory concentration (MIC). Among nineteen strains of bacteria tested, the antimicrobial activity of EA was the most potent against Proteus vulgaris showing MIC of 1.125 mg/ml. EA also inhibited the growth of the selected fungi at higher concentrations ranging from 7.5 mg/ml to 15.0 mg/ml. To investigate the effect of antimicrobial combinations of EA with naringenin, the fractional inhibitory concentration index (FICI) was determined by checkerboard assay for each strain. The antimicrobial combinations of EA with naringenin resulted in partial synergism against Staphylococcus aureus only, and showed additive effect in two strains including Klebsiella pneumoniae and Salmonella typhi. Antagonism was not found.

• Journal of Korean Society of Environmental Engineers
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• v.38 no.4
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• pp.162-168
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• 2016

The aim was to investigate the effect of reducing the odorous and complex odor released during the decomposition of food wastes using effective microorganism (EM) as a function of time at $20^{\circ}C$ and $35^{\circ}C$. The variation of total microbial counts and dominant species counts in EM and leachate produced during food wastes decomposition was also observed. In general, the cumulative concentration of sulfur compounds ($H_2S$, $CH_3SH$) and complex odor released during food wastes decomposition increased with increasing elapsed time. The nitrogen compounds ($NH_3$, trimethyl amine), however, was not observed in all samples. The addition of EM in food wastes resulted in the reduction of concentration of sulfur compounds and complex odor, in spite of the increase of $CH_3CHO$ concentration. The dominant microbial species detected in EM were Lactobacillus species(Lactobacillus rhamnosus and Lactobacillus casei). In the leachate produced during food wastes decomposition, however, the various microbial community alternative to that detected in EM was observed. The EM could be potentially useful as a tools for reducing odor induced from the food waste decomposition process.

• Journal of Korean Society of Environmental Engineers
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• v.30 no.10
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• pp.1006-1012
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• 2008

The effect of small and medium scale shipyard operations on environmental pollutions related with civil appeal of nearby residential areas was estimated. PM-10(particles with an aerodynamic diameter less than or equal to 10 $\mu$m) concentrations were higher, as the sampling sites were nearer from shipyards. PM-10 concentrations of sampling sites closer to shipbuilding companies were higher during the normal operation periods than on vacation at which only small works were done. The larger amount of dry deposition was observed as the sampling sites were closer to shipyards. The odor intensity was highly influenced by ambient temperature. Almost all odor intensities measured by air dilution sensory test went over the emission standard in summer. Odor properties measured by instrumental analysis were observed to exceed the emission standard enforced to the shipyards. Odor compounds such as ammonia, sulfur compounds and trimethylamine went over the standard. Concentrations of styrene and aldehydes were lower than the olfactory threshold. All equivalent sound levels measured at daytime except the value of SP-3 pont were in excess of the environmental standard. Almost all sound levels at nighttime in all measuring sites were higher than the environmental standard. The results of this study shows counterplans need to be established for reducing the civil appeal related pollutions in the research areas.

• Journal of the Korean Society of Marine Environment & Safety
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• v.18 no.1
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• pp.1-13
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• 2012

We measured various geochemical parameters, including the grain size, loss on ignition(LOI), total organic carbon(TOC), total nitrogen(TN), total sulfur(TS) and metallic elements, in surface sediment collected from 19 stations in Gamak Bay in April 2010 in order to understand the sedimentary types, the origin of organic matters, and the distribution patterns of alkali(Li, Na, K, Rb) and alkaline earth(Be, Mg, Ca, Sr, Ba) elements. The surface sediments were mainly composed of mud. The concentrations of Chlorophyll-a, TOC, TN, TS and LOI in sediment were the highest at the cultivation areas of fish and shellfish in the northern and southern parts of the bay. The redox potential(or oxidation-reduction potential) showed the positive value in the middle part of the bay, indicating that the surface sediment is under oxidized condition. The organic materials in sediment at almost all of stations were characterized by the autochthonous origin. Based on the overall distributions of metallic elements, it appears that the concentrations of alkali and alkaline earth elements except Ba in sediment are mainly influenced by the dilution effect of quartz. The concentrations of Sr and Ba are also dependent on the secondary factors such as the effect of calcium carbonate and the redox potential.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.17 no.5
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• pp.414-422
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• 1984

This research was conducted to evaluate the effect of polluted Oncheon Stream on the marine organisms in the Suyeong Bay. Water quality and 96 hr acute toxicity to the sea urchin, Hemicentrotns pulcherrimus by recirculation bioassay were examined from Feb. 20 to Apr. 15, 1984. The 96 hr $50\%$ effective concentration($EC_{50}$) on the attachment of the podia of the sea urchin was observed to occur at test concentrations between 40.0 and $51.0\%$ (v/v), and safe concentrations may be assumed to be within 4.0 and $5.1\%$. These values indicate as follows : 1. Oncheon Stream was extremely polluted by oxygen-demanding wastes and synthetic organic compounds from sewage and industrial waste water. 2. Linear alkylbenzene sulfonate(LAS) which has not been yet included in water quality standard was discharged above the TLm. 3. Unknown toxicity may be synergy among complex substances. In consideration of the relationship between COD values of Oncheon Stream and dilution water, the effect of toxicity of Oncheon Stream water reached to the area of the Suyeong Bay where the COD value was found to be 12.2 ppm.