• 한국식품과학회지
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• 제54권2호
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• pp.203-208
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• 2022

본 연구에서는 글루텐 분해능을 가지는 유산균을 선별하기 위해 전통식품인 젓갈을 이용하였다. 선별된 균주는 API, 16S rRNA sequencing과 현미경을 통해 간균의 형태를 갖는 Lactobacillus paracasei로 동정되었고 이를 GLU70으로 명명하기로 하였다. MRS 배지에서 GLU70의 생육특성을 조사한 결과, 6시간부터 12시간까지 대수기이며 12시간 이후부터 정지기임을 확인하였다. 이렇게 동정된 GLU70을 글루텐이 함유된 MRS배지에서 시간별로 배양하였을 때 대수기인 12시간 이후부터 24시간 이전까지 글루텐 분해가 가속화되었고, 48시간에서 최대 45% 정도의 분해율을 나타내었다. 또한 GLU70이 유산균으로서 적합하기 위해서 가져야하는 내산성, 내담즙성, 내열성에 대한 분석을 실시하였다. 많은 연구에서 Lactobacillus 종이 pH 2.5 이상에서는 균의 생존율이 높았지만, 그 이하에서는 거의 생존하지 않는다고 보고하고 있다 (Shin 등, 1999). 이에 따라 분리한 GLU70에서도 유사하게 pH 2.0에서는 균이 사멸하였고 pH 3.0부터 약 84%의 생존율을 보였고 많은 연구들과 유사한 결과를 가지는 것을 통해 우수한 내산성을 가지는 것을 확인하였다. 내담즙성의 경우 oxgall이 0.3% 함유된 배지에서 성장할 수 있을 정도의 내성을 가져야 한다는 연구결과와 비교하였을 때 GLU70은 담즙에 대하여 매우 강한 내성을 가지고 있다고 보기는 어렵다. 또한 내열성에 대한 연구결과에서는 50℃ 이상의 온도에서는 사멸하였으나 30℃에서는 높은 생존율을 나타냈다. 또한 분리된 GLU70이 실제 밀가루 반죽에서 감소하는 글루텐 함량을 분석하였을 때 12 h 배양한 배양상등액을 넣어 25℃에서 24 h 동안 발효한 밀가루 반죽에서 가장 높은 분해력을 확인하였다. 또한 밀가루 반죽의 발효 온도에 따른 글루텐 분해율을 타 균주와 비교하여 GLU70의 우수성을 판단하고자 하였다. GRAS 인정 유산균 11종과 GLU70을 각각 밀가루 반죽에 넣은 후 25℃와 35℃에서 발효하였다. 그 결과 GLU70이 타 균주들과 비교하였을 때 모든 온도에서 반죽이 흘러내리는 등의 이상현상 없이 50% 이상의 매우 높은 글루텐 분해율을 보였다. 이를 통해 GLU70이 향후 식품 산업에 다양한 활용 가능성을 지니며 글루텐 저감화 밀가루 제조 등에 적용하기에 적합하다고 제시된다.

• 대한약침학회지
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• 제18권1호
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• pp.72-78
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• 2015

Objectives: The stomach is a sensitive digestive organ that is susceptible to exogenous pathogens from the diet. In response to such pathogens, the stomach induces oxidative stress, which might be related to the development of both gastric organic disorders such as gastritis, gastric ulcers, and gastric cancer, and functional disorders such as functional dyspepsia. This study was accomplished to investigate the effect of Ganoderma lucidum pharmacopuncture (GLP) on chronic gastric ulcers in rats. Methods: The rats were divided into 4 groups of 8 animals each: the normal, the control, the normal saline (NP) and the GLP groups. In this study, the modified ethanol gastritis model was used. The rats were administrated 56% ethanol orally every other day. The dose of ethanol was 8 g/kg body weight. The normal group received the same amount of normal saline instead of ethanol. The NP and the GLP groups were treated with injection of saline and GLP respectively. The control group received no treatment. Two local acupoints CV12 (中脘) and ST36 (足三里) were used. All laboratory rats underwent treatment for 15 days. On last day, the rats were sacrificed and their stomachs were immediately excised. Results: Ulcers of the gastric mucosa appeared as elongated bands of hemorrhagic lesions parallel to the long axis of the stomach. In the NP and GLP groups, the injuries to the gastric mucosal injuries were not as severe as they were in the control group. Wound healings of the chronic gastric ulcers was promoted by using GLP and significant alterations of the indices in the gastric mucosa were observed. Such protection was demonstrated by gross appearance, histology and immunehistochemistry staining for Bcl-2-associated X (BAX), B-cell lymphoma 2 (Bcl-2) and Transforming growth factor-beta 1 (TGF-${\beta}1$). Conclusion: These results suggest that GLP at CV12 and ST36 can provide significant protection to the gastric mucosa against an ethanol induced chronic gastric ulcer.

• 동의생리병리학회지
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• 제16권1호
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• pp.62-66
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• 2002

Hypertension is not only a well-established cardiovascular risk factor but also increase the risk of atherosclerosis. Most studies conducted to investigate the effectiveness of treatment for cardiovascular disease such as hypertension have focused primarily on conventional drug and physiotherapeutic treatments. BanhabackchulChunma-tang(半夏白朮天麻湯:BCT) is popular herbal medicine used in clinic for the treatment of various symptoms of drulatory disorders and weakness of digestive system, including anorexa and nausea with vertigo, severe headache, vomiting and so on. However, the mechanisms underlying its efficacy are unknown. This study investigated the effects of BCT as an alternative medication on the contraction induced by phenylephrine and KCI in rat thoratic aorta. BCT revealed siginificant relaxation on phenylephrine-induced arterial contraction, but revealed noncompetitive effect on concentration responses of phenylephrine-induced contraction. Treatment of N-L/sup ω/ -argine methyl ester(L-NAME) and methylene blue(MB)(10/sup -5/M) reduced the relaxation of BCT. BCT also increased in vitro NO production. It suggest that the relaxation effect of BBT is related with NO pathway, becausse the effect of L-NAME and MB are due to inhibition of NO synthesis from endothelial cells. These results indicate that BCT would be effective in hypertension treatment and its mechanism of relaxtion on arterial contraction is likely to be related with NO production, blocking of α-receptor and signal transduction after receptor activation.

• 대한한방소아과학회지
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• 제29권4호
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• pp.67-76
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• 2015

Objectives The purpose of this review is to investigate the domestic and foreign studies of pediatric Chuna treatment and propose the directions of future studies and clinical applications. Methods We searched for the study at RISS, KISS, DBPIA, Pubmed, CNKI by keywords, '추나', 'Osteopathic', 'Chiropractic', 'Manipulation', '推拿', '導引', '按摩', After 2010. Results 1. Selected 3 domestic studies were categorized as 1 survey study and 2 case reports. Selected 41 foreign studies from Pubmed were categorized as 15 systemic reviews, 8 survey studies, 12 case reports and 6 control studies. Selected 82 foreign studies from CNKI were categorized as 10 systemic reviews, 22 case reports and 50 control studies. 2. 2 clinical domestic studies researched on idiopathic Scoliosis. The foreign clinical studies from Pubmed are 18 cases, and those studies were categorized into Premature baby care (3), Infant colic (2), ADHD (2), Congenital talipes equinovarus (1), Somatic dysfuntion (1), Nonsynostotic occipital plagiocephaly (1), Conversion disorder (1), Lower back pain (1), Chronic bilateral dorsal foot pain and stiffness (1), plantar fasciitis (1), Migraine headaches (1), Cyclic vomiting syndrome (1), Acute otitis media (1) and Cerebral palsy (1). The other 72 foreign clinical studies were from CNKI, and they studied 39 different diseases. Systematically, they studied about digestive diseases (25), respiratory diseases (20), fever (6), musculoskeletal diseases (5), nervous system diseases (5), dermatology diseases (2) and other disease states. The Chuna treatment was used in variety of studies. 3. 2 clinical domestic studies adopted techniques of Osteopathy Chuna. The foreign clinical studies from Pubmed adopted techniques of Osteopathic manipulation (10) and Chiropractic manipulation (8). The other foreign clinical studies from CNKI adopted techniques of Acupressure (69), Abdominal manipulation (23), Spinal manipulation (21), Thoracic manipulation (11), Traction manipulation (2), Muscular manipulation (2), Squeezing Sha manipulation (1), Spine correction (1), Joint manipulation (1) and Fascia manipulation (1). Conclusions In addition to musculoskeletal disorders, variety of pediatric diseases could be treated with Chuna treatment instead of acupuncture.

• 대한수의학회지
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• 제50권4호
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• pp.265-271
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• 2010

Post-weaning multisystemic wasting syndrome (PMWS) is a new emerging disease affecting nursery and growing pigs in worldwide. Porcine circovirus type 2 (PCV-2) is a most important pathogen associated with PMWS. This study was carried out to investigate the pathological changes in the pancreas of pigs diagnosed as PMWS. To detect the PCV-2 antigen and nucleic acid in the tissue, immunohistochemistry and polymerase chain reaction (PCR) was conducted, respectively. 24 pigs of 4-10 weeks old showed clinical signs of PMWS such as chronic wasting, respiratory distress and diarrhea were examined. Histopathologically, interstitial and periductular mononuclear cells infiltration were observed in pancreas. Multifocal to diffuse necrosis of acinar tissues or necrotizing to granulomatous pancreastitis with numerous syncytial cells infiltration were examined in severe cases. PCV-2 nucleic acid was detected from all tested pancreas using PCR. The PCV-2 antigen in 12 pancreas sections was detected by immunohistochemical staining. PCV-2 has a tropism for vascular endothelial cells and infiltrated macrophages. Although gross lesions are uncommon in the pancreas of pigs with PMWS, histopathological changes and the presence of PCV-2 in this tissue may be related to clinical signs associated with digestive disorders.

• 대한한의학방제학회지
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• 제23권1호
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• pp.161-169
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• 2015

Objectives : This study is aimed to report the outcomes of the treatment of a patient with chronic anal fissure during a 8-year period. Methods : Basic patient’s data was analyzed as a CARE guideline. And related psychological state was measured with BAI, STAI-X-II test. Results : The treatment is based on Shanghanlun and the patient reported better outcomes. The main symptoms of the patient were chronic constipation, blood in the stool. The main clinical findings were that the patient’s symptoms got worse during the examination period because of the anxiety of the exam. And loss of appetite and digestive disorders are due to Jue-Yin-Bing(厥陰病) and the clue of bleeding can be found in article 375 in Shanghanlun. The main diagnoses was chronic anal fissure and main interventions were Chijasi-tang according to article 375 in Shanghanlun and counseling on anxiety. As a results bleeding has stopped after taking herbal medicine and constipation has been treated at the same time. After 6 months, the patient performed BAI, STAI-X-II test and scores got lowered indicating decreasing anxiety level. Conclusion : Chijasi-tang with counseling achieved valid positive results to the patient with chronic anal fissure.

• 대한한방내과학회지
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• 제31권3호
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• pp.539-553
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• 2010

Objectives : The object of this study was to obtain accurate information (single oral dose toxicity) of Lonicerae Flos (LF; Dried flower bud parts of Lonicera japonica Thunb (Caprifoliaceae)), which has traditionally been used in Korean medicine for treating various inflammatory diseases. Methods : In order to observe the 50% lethal dose (LD 50), approximate lethal dosage (ALD) and target organs, test articles were once orally administered to female and male ICR mice at dose levels of 2,000, 1,000, 500 and 0 (control) mg/kg (body weight.). The mortality and changes on body weight, clinical signs and gross observation were monitored for 14 days after single oral treatment of LF aqueous extracts with organ weights and histopathological observations of 12 types of principle organs. Results : 1. After single oral treatment of LF aqueous extracts, we could not find any mortality and toxicological evidences up to 2,000 mg/kg treated group, the limited dosages in rodents at body and organ weights, clinical signs, gross and histopathological observations. 2. Slight diarrhea was detected in most mice treated with 2,000 mg/kg of LF aqueous extracts and male mice of LF aqueous extracts 1,000 mg/kg within 2 days after end of treatment, respectively. Conclusion : The results obtained in this study suggest that the LD 50 and ALD of LF aqueous extracts in both female and male mice after single oral treatment were considered as over 2,000 mg/kg because no mortalities were detected up to 2000 mg/kg, the highest dose recommended by KFDA and OECD. However, we also observed the possibility of digestive disorders like diarrhea when over 1,000 mg/kg of LF aqueous extracts were administered in the present study.

• 대한한방소아과학회지
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• 제17권1호
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• pp.117-129
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• 2003

We made a clinical analysis of 135 pediatric patients who visited Emergency Room of Dongguk University Oriental Hospital, during 1 year from March 2002 to February 2003. The results were as follows ; 1. Whether they received medical examination and treatment or not : new patients were 95(70.4%) and pediatric patients who received medical examination and treatment were 40(29.6%). 2. Seasonal distribution: Spring(March, April, May) was 45(33.3%), Summer(June, July, August) was 35(25.9%), Autumn(September, October, November) was 32(23.7%) and Winter(December, January, February) was 23(17.0%). 3. The time interval between onset and arrival : within 6 hours were 68(50.4%), 6-12 hours were 14(10.4%), 12-24 hours were 26(9.3%), 24-48 hours were 17(12.6%), 48-72 hours were 6(4.4), over 72 hours were 4(3.0%). 4. Whether they went through other hospitals or not : pediatric patients who didn't go through other hospitals were 105(77.8%), pediatric patients who went through other hospitals were 30(22.2%). 5. Systemic distribution of diseases: Infectious diseases were 1(0.7%), Physique diseases were 4(3.0%), Digestive diseases were 73(54.1), Nervous also Mental diseases were 32(23.7%), Cardiovascular diseases were 2(1.5%), Skin disorders were 1(0.7%), Respiratory diseases were 22(16.3%). 6. Medical care : Acupuncture and moxibustion were 1(0.7%), moxibustion and medication were 1(0.7%), acupuncture, moxibustion and medication were 1(0.7%), venesection was 24(17.8% ), venesection and medication were 17(12.6%), consultation was 16(11.9%), medication was 44(32.6%), acupuncture was 3(2.2%), acupuncture and medication were 14(10.4%) and transferred out patients were 14(10.4%). 7. Revisit: revisit patients were 30(22.2%).

• 사상체질의학회지
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• 제23권3호
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• pp.361-373
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• 2011

1. Objectives: The purpose of this study was done to learn the Sasang constitutional distribution and to find out if there are differences in the type of diseases and symptoms according to the Sasang constitution in Japan. 2. Methods: We collected data from 366 patients who visited the Department of Oriental Medicine, Keio University and recruited 132 healthy persons in Tokyo, Japan. For sasang constitution diagnosis, they all have done SSCQ-P(Sasang Constitution Questionaire for Patients) questionnaire. and a sasang constitution specialist diagnosed the sasang constitution of them. And We classify the diseases and symptoms of 313 patients according to KCD(Korean Standard Classification of Diseases) and learn the prevalences of diseases and symptoms according to Sasang Constitution. 3. Results: 1) Among the total 498 subjects, distributional rate of Taeyangin, Soyangin, Taeeumin, and Soeumin were 2.0%, 26.3%, 29.9%, and 41.8%. Among the 366 patients, distributional rate of Taeyangin, Soyangin, Taeumin, and Soeumin were 0.8%, 27.3%, 28.7%, and 43.2%. Among the 132 healthy group, distributional rate of Taeyangin, Soyangin, Taeeumin, and Soeumin were 5.3%, 23.5%, 33.3%, and 37.9%. 2) The prevalences of 'V.Mental and behavioural disorders', 'XI.Diseases of the digestive system', 'XV.Pregnancy, childbirth and the puerperium' and 'feeling of coldness(X VIII.Symptoms, signs and abnormal clinical and laboratory findings, NEC)' of Soeumin were significantly higher than those of the other constitutions.(p-value<0.05) 4. Conclusions: The distributional rate of Sasangin of Japanese was different from that of Korean and especially the distributional rate of Soeumin of Japanese was significantly higher than that of Korean. There were significant differences on the prevalences of some diseases and symtoms according to KCD in Soeumin.

• Asian Pacific Journal of Cancer Prevention
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• 제16권17호
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• pp.7561-7566
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• 2015

Ziziphus jujuba (ZJ) fruit is rich in bioactive functional components such as polysaccharides, triterpenoid acid, flavonoids and oleamide. It has been commonly used in the treatment of various diseases including diabetes, digestive disorders, diarrhea, skin infections, liver and urinary diseases. However, its dietary effect on chemoprevention of colon cancer has never been studied. The present study was to evaluate the protective effects of dietary ZJ on colitis-associated colon carcinogenesis in azoxymethane (AOM)-dextran sodium sulphate (DSS)-treated mice. AOM was injected (10 mg/kg b.wt., i.p.) and three cycles of 2% DSS in drinking water for 7 days with 14 days of normal drinking water in-between was administered to induce colitis-associated colon cancer. ZJ fruit was supplemented in feed as 5 and 10%. Dietary ZJ significantly attenuated aberrant crypt foci (ACF) formation thereby decreasing the progression of hyperplasia to dysplasia. In addition, it significantly reduced circulating white blood cells, lymphocytes, neutrophils, monocytes, eosinophils, basophils and platelets compared to colon cancer mice. We conclude that ZJ supplementation delayed the progression of colon cancer from hyperplasia to dysplasia and ultimately adenocarcinoma and cancer. In addition, it decreased circulating tumor-related leucocytes, main regulators of cancer inflammation. Therefore, dietary consumption of ZJ fruit attenuated the formation of ACF and delayed the progression of colon cancer.