• Title/Summary/Keyword: Differentiation of Self

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Growth factors improve the proliferation of Jeju black pig muscle cells by regulating myogenic differentiation 1 and growth-related genes

  • Park, Jinryong;Lee, Jeongeun;Song, Ki-Duk;Kim, Sung-Jo;Kim, Dae Cheol;Lee, Sang Cheol;Son, Young June;Choi, Hyun Woo;Shim, Kwanseob
    • Animal Bioscience
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    • v.34 no.8
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    • pp.1392-1402
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    • 2021
  • Objective: The growth rate of pigs is related to differentiation and proliferation of muscle cells, which are regulated by growth factors and expression of growth-related genes. Thus, the objective of this study was to establish optimal culture conditions for Jeju black pig (JBP) muscle cells and determine the relationship of various factors involved in muscle growth with the proliferation of JBP muscle cells. Methods: Muscles were taken from the femur skeletal muscle of JBP embryos. After isolation of the muscle cells, cells were cultured in a 6-well plate under four different culture conditions to optimize culture conditions for JBP muscle cells. To analyze proliferation rate of JBP muscle cells, these muscle cells were seeded into 6-well plates at a density of 1.5×105 cells per well and cultured for 3 days. Western blot and quantitative real-time polymerase chain reaction were applied to verify the myogenic differentiation 1 (MyoD) expression and growth-related gene expression in JBP muscle cells, respectively. Results: We established a muscle cell line from JBP embryos and optimized its culture conditions. These muscle cells were positive for MyoD, but not for paired box 7. The proliferation rate of these muscle cells was significantly higher in a culture medium containing bFGF and epidermal growth factor + basic fibroblast growth factor (EGF+bFGF) than that without a growth factor or containing EGF alone. Treatment with EGF and bFGF significantly induced the expression of MyoD protein, an important transcription factor in muscle cells. Moreover, we checked the changes of expression of growth-related genes in JBP muscle cells by presence or absence of growth factors. Expression level of collagen type XXI alpha 1 gene was changed only when EGF and bFGF were added together to culture media for JBP muscle cells. Conclusion: Concurrent use of EGF and bFGF increased the expression of MyoD protein, thus regulating the proliferation of JBP muscle cells and the expression of growth-related genes.

Genetic diversity and geographic differentiation in the endangered Primula farinosa subsp. modesta, a subalpine endemic to Korea (한반도 아고산대 특산·희귀식물 설앵초의 유전적 다양성과 지리적 분화)

  • Chung, Jae-Min;Son, Sung-Won;Kim, Sang-Yong;Park, Gwang-Woo;Kim, Sung-Shik
    • Korean Journal of Plant Taxonomy
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    • v.43 no.3
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    • pp.236-243
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    • 2013
  • Many plant species in subalpine regions are under threat of extinction as a result of climate change. In this study, the genetic diversity and geographic differentiation of three regions and six populations of Primula farinosa subsp. modesta (Bisset & Moore) Pax in Korea were assessed using the ISSR (Inter Simple Sequence Repeat) marker. The average genetic diversity (P = 60.62, SI = 0.299, h = 0.190) was relatively lower than that of other long-lived perennials, even though it is a self-incompatible species. AMOVA analysis showed that 50% of the total genetic diversity was partitioned among regions and Bayesian cluster analysis showed some remarkable geographic trends that were structured into 2 or 3 regions, suggesting limited gene flow among regions. Considering the population fragmentation, low level genetic diversity, and high genetic differentiation, it is essential to establish in situ and ex situ conservation strategies for P. farinosa subsp. modesta.

Differentiation of Risk Factors between Female Collegiate Athletes and non-Athletes with Eating Disorders/Disordered Eating (섭식장애/이상섭식 여자대학운동선수와 일반여자대학생의 특성 및 위험요인)

  • Jeong, Chankyo;Kang, Hyung-Sook
    • 한국체육학회지인문사회과학편
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    • v.54 no.1
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    • pp.619-629
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    • 2015
  • The purpose of this study was to identify the characteristics between athletes and non-athletes with eating disorders (ED)/disordered eating(DE) and to investigate predictors for ED/DE. 376 female collegiate athletes and 567 college women participated in the study. After using subject selection criteria to detect ED/DE, 243 athletes were considered as either ED (n=22) or DE (n=221). In the non-athletes group, 37 cases of ED and 242 cases of DE were detected. Along with the EDEQ and EDI-2, participants completed a demographic questionnaire, SATAQ, and RSES. The results indicated that the mean seperation between the two groups with ED existed in seven variables including BMI perceived, BMI ideal, EDEQ mean, eating scale, weight scale, perfectionism, and self-esteem. In addition, the means of six variables including BMI perceived, BMI ideal, perfectionism, SATAQ score, awareness, and self-esteem were significantly different between the two groups with DE. Significant predictors for ED/DE were drive for thinness and self-esteem in the non athletes while drive for thinness, body dissatisfaction, self-esteem, internalization, and perfectionism were the predictors in the athletes within the ED/DE group. The results suggest that drive for thinness and self-esteem were the essential predictors for ED/DE in the two groups. in the athletes with ED/DE, the predictors were more complicated than those in the non-athletes with ED/DE.

A Case of Ramsay Hunt syndrome with Poor Prognosis on EMG (근전도상 불량한 예후를 보였던 Ramsay Hunt Syndrome 환아 1례에 대한 임상적 고찰)

  • Koh, Duck-Jae;You, Han-Jung;Cho, Hyung-Jun;Kim, Deog-Gon;Lee, Jin-Yong
    • The Journal of Pediatrics of Korean Medicine
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    • v.18 no.2
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    • pp.127-142
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    • 2004
  • Objective : To evaluate the effect of Oriental medical treatment on a patient with Ramsay Hunt syndrome with poor prognosis on EMG. Methods : We applied various methods of Oriental Medical treatment including Acupuncture, Electroacupuncture, Herb medicine, Vesiculation therapy using Mylabris and Crontonis Fructus, Massage, Self-excercise of face muscles. Results : 1. Herb medicine was applied on the basis of Differentiation of Syndromes(辨證): We tried Herb medicine to improve the function of Digestive System. The patient's stool condition, subjective sensation related with abdominal pain and general condition improved. 2. We used the Vesiculation therapy using Mylabris which was used traditionally to treat Facial Palsy. We powdered Mylabris and mixed it with Crontonis Fructus to make a paste. We put it on Ye-poong(?風), Nae-gwan(內關) at left side. It induced vesicles at the applied area, but it disappeared in 48 hours without any scar. 3. We applied acupuncture, electroacupunture, massage and self-excercise of face muscles. On gross scale assessment, there was some improvement. And assessment with regional scale also showed changes. The patient and the guardian were satisfied with the result. Conclusion : We experienced a case of Ramsay Hunt syndrome with poor prognosis on EMG. She also showed poor response to conservative treatment. Through various modalities of Oriental medical treatment, we attained some clinical improvement.

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Analysis of Designation and Symbolic Meanings of Floral Emblems in South Korea as Elements of Garden Tourism and Design

  • Kim, Inhea;Park, Jin-Sil;Choi, Kyoung-Ok
    • Journal of People, Plants, and Environment
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    • v.23 no.1
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    • pp.87-99
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    • 2020
  • This study was conducted to analyze the current state and symbolic meanings of floral emblem designation in local governments of South Korea. The scope of local governments subject to analysis was limited to special city, metropolitan city, province, self-governing province, city, self-governing city, county and district based on their administrative divisions. The floral emblems of local governments and their symbolic meanings as of 2019 were examined. A total of 44 plant species were designated as floral emblems. Many plant species with high designation frequency were included in Rhododendron spp., Rosa spp., Camellia spp., Magnolia spp., and Prunus spp.. Plant species with higher designation frequency tended to have more symbolic meanings. A total of 155 terms were used for the symbolic meanings assigned to all the designated floral emblems. The major symbolic meanings were relevant to material affluence or economic growth, community spirit, and personality generally required from local residents. Most of the plant species linked to the top 10 most frequently assigned terms in symbolic meaning were those of the top 10 most frequently designated floral emblems. In the case of floral emblems with high designation frequency, it was shown that they were linked with various symbolic meanings in order to grant identity with regional distinctiveness and differentiation, regardless of the symbolic meaning that the designated flowers generally have. However, the floral emblems with low designation frequency seem to have relatively strong physical or emotional relevance with local governments and thus are expected to have high utilization in regional branding and tourism marketing.

Consumer Perceptions on SST in Retail Atmosphere: An application of S-O-R framework

  • BYUN, Sookeun;HA, Yongsoo
    • Journal of Distribution Science
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    • v.18 no.3
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    • pp.87-97
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    • 2020
  • Purpose: The aim of this study is to understand the internal and external responses that consumers experience when they are exposed to an innovative system in retail stores. This study considered the SST(Self-Service Technology) system in a retail setting as a type of functional environmental stimuli and selected a smart shopping cart as an example of SST system. The influences of functional environmental stimuli on consumers' emotional, cognitive, and behavioral responses were examined by applying S-O-R model. In addition, this study attempted to extend the traditional S-O-R model by (a) incorporating personal characteristics variables such as time pressure and perceived crowding and (b) considering not only emotional but also cognitive aspects of consumers' internal responses. Research Design, Data, and Methodology: This study used a video-scenario technique. Participants watched a video about grocery shopping situations using a smart shopping cart and responded to their emotional, cognitive, and behavioral responses. An online survey was conducted using Amazon's Mechanical Turk (N = 185). All participants were US consumers over 20 years old and had been shopping at the grocery store in the last month. Data were analyzed through structural equations modeling with AMOS 20. Results: Test results showed that consumers who perceived higher levels of time pressure and perceived crowding in usual shopping situations were more likely to evaluate the SST system favorably. The results showed that personal characteristics have a significant impact on consumers' evaluation of functional environmental stimuli in retail setting. As consumers evaluated the SST system favorably, they experienced more positive affect and less negative affect during their shopping behaviors. Positive affect led to good service quality inference, which further increased patronize intention. However, negative affect did not show a significant impact on service quality inference, but only on patronize intention. Conclusions: This study attempted to investigate the influence of SST system by extending the traditional S-O-R model. This study classified the SST system as functional environmental stimulus of retail stores and analyzed the effect of stimulus on consumers' internal and external responses. The results of this study showed that the introduction of innovative SST can serve as an effective store differentiation strategy in an increasingly competitive retail environment.

The Influence of New Seniors' Lifestyle and Apparel Purchase Criteria on Design Preference of Outdoor Apparel Products (뉴시니어 라이프스타일과 의복구매기준이 아웃도어 의류제품 디자인 선호도에 미치는 영향)

  • Ji, Kyoungha;Kim, Hanna
    • Journal of Fashion Business
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    • v.21 no.4
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    • pp.73-89
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    • 2017
  • This research aimed to identify the characteristics of outdoor apparel market for new seniors. To achieve this goal, influence of new seniors' lifestyle and apparel purchase criteria of design preferences of outdoor apparel products were investigated. An online-survey was conducted targeting fifties who have bought outdoor apparel within one year. The collected data were carried out for factor analysis, independent sample T-test, and regression analysis by using SPSS 22.0. Results showed that new seniors' lifestyles were composed of appearance-orientation, well-being diet, well-being exercise, self-development, and challenge-orientation. Purchase criteria of outdoor apparels were factored out as practical, aesthetic, and social. Appearance -orientation and self-development affect aesthetic and social factors. Well-being diet influences all factors of purchase criteria. Challenge -orientation has effect only on social factor. Women favored round-and V-neckline more than men did as well as preferred half and 3/4 long for sleeve. Customers who value practical purchase criteria preferred half-sleeve, zipper, and button-closure designs. In contrast, customers who have aesthetic purchase criteria like round/V-neckline and cap sleeve. Social factor affected high neckline and vivid-tone color preference significantly. As outdoor design for new senior it is critical to introduce differentiation of outdoor brand with various designs combining functionalities of outdoor activities and style looking young and dandy, which is highlighted as trendy and casual, over current outdoor apparels' unified design.

Virtual Reality and the Space of Gu-Wun-Mong (사이버공간과 『구운몽』의 세계)

  • Jeon, Yi-Jeong
    • The Journal of the Korea Contents Association
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    • v.11 no.6
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    • pp.90-97
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    • 2011
  • The availability of computers and the wide network of the internet of the 'Information Society' has created a new space: Cyberspace. In this situations, the matters of individual identities and morality are more important problems. Korean classical novel, Gu Wun Mong, embodied the exploration process of identities in virtual spaces, has gravity of a situation. Seong Jin, the hero experienced the life of Yang so you went throughing virtual life, be born again. And he met eight taoist fairy in that virtual world. In this process, Seong Jin overcame a dualistic world of view and established identity of genuine truth-seeker. The 'Dream-fantasy' experience differentiation real world and virture world and give to Seong Jin a new identity, So You. Seong Jin used avatar, So You was free from limits of self and became obtain multiple personalities. Finally, Seong Jin realized the fact that real world and virtual one is the same, for development self-perception. Seong Jin's enlightment provided solutions for modern netizens, who is ambivalent about contradiction the real and virtual, infinity and clash of desire.

The Interaction Effects between News Frames and Community Structure on Vote Choice (지역공동체 구조와 뉴스프레임이 투표행위에 미치는 영향)

  • Park, Cheong-Yi
    • Korean journal of communication and information
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    • v.17
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    • pp.37-60
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    • 2001
  • This study attempted to demonstrate the interaction effects between attitudinal frames of nine daily newspapers and community structure in the 1994s Michigan gubernatorial election. It was theoretically guided by framing research and the self-presentation theory of social-cognition perspective and empirically tested with archival data. For the purpose of this study, content analysis of nine statewide daily newspapers was employed in order to provide data on news framing. Data on voting rates for John Engler, winner of the 1994 Michigan Gubernatorial election, in each county of Michigan were used for vote choice while Michigan census data were used for constructing an Index of community structural differentiation. The results indicated that majority compliance frames were slightly more related with vote choice in homogeneous com-unities rather than were majority compliance frames in heterogeneous communities while social identification frames tended to have an influence on vote choice in heterogeneous communities more than did social identification frames in homogeneous communities.

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An Automatic Smile Analysis System for Smile Self-training (자가 미소 훈련을 위한 자동 미소 분석 시스템)

  • Song, Won-Chang;Kang, Sun-Kyung;Jung, Tae-Sung
    • Journal of Korea Multimedia Society
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    • v.14 no.11
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    • pp.1373-1382
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    • 2011
  • In this study, we propose an automated smile analysis system for self smile training. The proposed system detects the face area from the input image with the AdaBoost algorithm, followed by identifying facial features based on the face shape model generated by using an ASM(active shpae model). Once facial features are identified, the lip line and teeth area necessary for smile analysis are detected. It is necessary to judge the relationship between the lip line and teeth for smiling degree analysis, and to this end, the second differentiation of the teeth image is carried out, and then individual the teeth areas are identified by means of histogram projection on the vertical axis and horizontal axis. An analysis of the lip line and individual the teeth areas allows for an automated analysis of smiling degree of users, enabling users to check their smiling degree on a real time basis. The developed system in this study exhibited an error of 8.6% or below, compared to previous smile analysis results released by dental clinics for smile training, and it is expected to be used directly by users for smile training.