• International Journal of Oral Biology
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• v.37 no.4
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• pp.167-173
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• 2012

This study investigated the genes involved in the differentiation of odontoblasts derived from human dental pulp stem cells (hDPSCs). hDPSCs isolated from human tooth pulp were validated by fluorescence activated cell sorting (FACS). After odontogenic induction, hDPSCs were analyzed investigated by Alizaline red-S staining, ALP assay, ALP staining and RT-PCR. Differential display-polymerase chain reaction (DD-PCR) was performed to screen differentially expressed genes involved in the differentiation of hDPSCs. By FACS analysis, the stem cell markers CD24 and CD44 were found to be highly expressed in hDPSCs. When hDPSCs were treated with agents such as ${\beta}$-glycerophosphate (${\beta}$-GP) and ascorbic acid (AA), nodule formation was exhibited within six weeks. The ALP activity of hDPSCs was found to elevate over time, with a detectable up-regulation at 14 days after odontogenic induction. RT-PCR analysis revealed that dentin sialophosphoprotein (DSPP) and osteocalcin (OC) expression had increased in a time-dependent manner in the induction culture. Through the use of DD-PCR, several genes were differentially detected following the odontogenic induction. These results suggest that these genes may possibly be linked to a variety of cellular process during odontogenesis. Furthermore, the characterization of these regulated genes during odontogenic induction will likely provide valuable new insights into the functions of odontoblasts.

• Proceedings of the Korean Geotechical Society Conference
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• 2006.03a
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• pp.268-275
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• 2006

Excavation works of cylindrical shafts and tunnels for the construction of a variety of infrastructures have been frequently going on in the urban areas. When ground excavations of cylindrical shafts and shallow tunnels proceed in the ground condition of high water level and silt particle component, ground water drawdown involving soil particle migration causes loosening of ground around tunnels and shafts, causes settlement and deformation of ground. Damages due to ground sinking and differential settlement can occur in the adjacent ground and structures. The extent and possibility of damage relevant to ground water drawdown and soil particle migration can't be so precisely expected in advance that we will face terrible damages in case of minor carefulness. This paper introduces two examples of construction management where using incremental deformation graph of inclinometer, we noticed the possibility of soil migration due to ground water drawdown in the excavation process of vertical shaft and shallow tunnel, analysed a series of measurement data in coupled connection, properly prepared countermeasures, so came into safe and successful completion of excavation work without terrible damages. The effort of this article aims to improve and develop the technique of design and construction in the coming projects having similar ground condition and supporting method.

• Journal of the Korean Society for Nondestructive Testing
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• v.22 no.3
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• pp.261-266
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• 2002

Up to now a wide variety of researches on inpipe robots for inspection have been introduced, but it still seems to be difficult to construct a robot providing mobility sufficient to navigate inside the complicated configuration of underground pipelines. The robot for the inspection of pipelines should freely move along the basic configuration of pipelines such as along horizontal or vertical pipelines. Moreover it should be able to travel along reducers and elbows, and especially the capability for steering in branches is essential to it. In this report, citical points and technologies in the development of the inpipe inspection robots are introduced and inpipe robots developed for last several years are introduced.

• Coupled systems mechanics
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• v.3 no.3
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• pp.267-289
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• 2014

A proper physical modeling of infilled building frame-foundation beam-soil mass interaction system is needed to predict more realistic and accurate structural behavior under static vertical loading. This is achieved via finite element method considering the superstructure, foundation and soil mass as a single integral compatible structural unit. The physical modelling is achieved via use of finite element method, which requires the use of variety of isoparametric elements with different degrees of freedom. The unbounded domain of the soil mass has been discretized with coupled finite-infinite elements to achieve computational economy. The nonlinearity of soil mass plays an important role in the redistribution of forces in the superstructure. The nonlinear behaviour of the soil mass is modeled using hyperbolic model. The incremental-iterative nonlinear solution algorithm has been adopted for carrying out the nonlinear elastic interaction analysis of a two-bay two-storey infilled building frame. The frame and the infill have been considered to behave in linear elastic manner, whereas the subsoil in nonlinear elastic manner. In this paper, the computational methodology adopted for nonlinear soil-structure interaction analysis of infilled frame-foundation-soil system has been presented.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.35 no.3
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• pp.171-177
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• 2009

This study described formation and dispersion of stable lamellar structure containing ceramide. To characterize of the lamellar structure containing ceramide, we used differential scanning calorimetry, x-ray diffraction, and FT-IR spectra, which enabled us to demonstrate that ceramide with appropriate amounts of lipid, oil and surfactant can assemble to form a stable lamellar-phase. These approaches to use ceramide offers useful means to fabricate a variety of biocompatible emulsion and liposome, which enlarge their applicability in the fields of drug delivery, dermatology and cosmetics.

• Geomechanics and Engineering
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• v.20 no.6
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• pp.497-503
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• 2020

In the Kingdom of Saudi Arabia, the shallower depth of the earth's crust is composed of loose dune or beach sand with soluble salts. The expansive behavior of salt bearing soil, fluctuation of ground water table and extreme environmental conditions offer a variety of geotechnical problems affecting safety and serviceability of the infrastructure built on it. Despite spending money, time and other resources on repair and rehabilitation, no significant attention is paid to explore the root causes of excessive differential settlement and cracking to these facilities. The scientific solution required to ensure safety and serviceability of the constructed infrastructure is to improve the strength and durability properties of the supporting ground. In this study, shredded plastic is employed as a low cost and locally available additive to improve strength characteristics of the desert sand. The study shows a remarkable increase in the shear strength and normal settlement of the soil. A seven (07) degree increase in angle of internal friction is achieved by adding 0.4 percent of the shredded plastic additive. The effect of different proportions and sizes of the plastic strips is also investigated to obtain optimum values. Such a long-lived solution will seek to reduce maintenance and repair costs of the infrastructure facilities laid on problematic soil along with reduction of environmental pollutants.

• Clinical and Experimental Pediatrics
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• v.63 no.8
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• pp.291-300
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• 2020

Advances in autoimmune encephalitis studies in the past 10 years have led to the identification of new syndromes and biomarkers that have transformed the diagnostic approach to the disorder. The disorder or syndrome has been linked to a wide variety of pathologic processes associated with the neuron-specific autoantibodies targeting intracellular and plasma membrane antigens. However, current criteria for autoimmune encephalitis are quite dependent on antibody testing and responses to immunotherapy, which might delay the diagnosis. This form of encephalitis can involve the multifaceted presentation of seizures and unexpected behavioral changes. The spectrum of neuropsychiatric symptoms in children is less definitive than that in adults, and the incorporation of clinical, immunological, electrophysiological, and neuroradiological results is critical to the diagnostic approach. In this review, we document the clinical and immunologic characteristics of autoimmune encephalitis known to date, with the goal of helping clinicians in differential diagnosis and to provide prompt and effective treatment.

• Preventive Nutrition and Food Science
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• v.11 no.4
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• pp.273-277
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• 2006

Recently, Rhei Rhizoma extracts (RRE) have begun to receive more attention as potential biological response modifiers. In the present study, we studied the antiproliferative effect of RRE on tumor cells and the effect of RRE on macrophage function. A variety of tumor cells and macrophages were treated with RRE at various concentrations. The effect of RRE on cell proliferation was measured by MTT assay and the effect of RRE on the production of nitric oxide (NO) was determined in the macrophage-like cell lines Raw264.7, C6 and peritoneal macrophages (pMQ). RRE inhibited the growth of tumor cells (e.g., B16, HOS). However, the effects of RRE on the production of NO varied with macrophage types. RRE had no effect on C6 cell growth and slightly increased the growth of Raw264.7 cells. In addition, treatment of normal pMQ with RRE enhanced NO production in a concentration-dependent manner, whereas RRE suppressed NO production at $50\;{\mu}g/mL$ in both Raw264.7 and C6 cells. However, RRE suppressed NO production in LPS/IFN-$\gamma$-stimulated C6 cells. Overall, these results suggest that RRE elicits an antiproliferative property and differentially modulates NO production in various macrophages, and have a potential for therapeutic application.

• Journal of the Korean Institute of Landscape Architecture
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• v.35 no.4
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• pp.105-118
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• 2007

The purpose of this study is not only to compare between 'Opened Landscape' and 'Filtered Landscape' image and preference but also to suggests a guide line of planting design for progressive realization. For this, the image structures of photo-sketch simulation for parallax landscape have been investigated by Semantic Differential scale(S.D. scale) and the Factor analysis. The results could be summarized as follows. The results of S.D. scale values for landscape through parallax were greater than non-parallax landscape. The scenes through parallax were better preferred to direct view. Thus the results of photo-sketch simulation test support the expected hypothesis that the visual environment of complexity and variety is closely correlated with the parallax effect and monotonous or non-parallax environment, and parallax effect on close view more bigger than the distant view. Factors covering the spatial image of parallax landscape were found to be seven and Total values were 60.35 %. The most important factors determining the parallax effect were Factors I 'depth of space' and VI 'expectation of space and interest'. An outstanding view must be handled properly to be preserved or accentuated. In this sense, the parallax spatial beauty with tree could be improved through the visual aspects of plan arrangements and the progressive realization appeared to be one effective design technique for landscape planning and design.

• Research in Plant Disease
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• v.20 no.3
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• pp.182-188
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• 2014

Because bacterial isolates from only a few genera have been developed commercially as biopesticides, discovery and characterization of novel bacterial strains will be a key to market expansion. Our previous screen using plant bioassays identified 24 novel biocontrol isolates representing 12 different genera. In this study, we characterized the 3 isolates showing the best biocontrol activities. The isolates were Pantoea dispersa WCU35, Proteus myxofaciens WCU244, and Exiguobacterium acetylicum WCU292 based on 16S rRNA sequence analysis. The isolates showed differential production of extracellular enzymes, antimicrobial activity against various fungal or bacterial plant pathogens, and induced systemic resistance activity against tomato gray mold disease caused by Botrytis cinerea. E. acetylicum WCU292 lacked strong in vitro antimicrobial activity against plant pathogens, but induced systemic resistance against tomato gray mold disease. These results confirm that the trait of biological control is found in a wide variety of bacterial genera.