• The Korean journal of internal medicine
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• v.33 no.6
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• pp.1234-1240
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• 2018

Background/Aims: Red blood cell distribution width (RDW) is a value representing the heterogeneity in the size of red blood cell, and it is usually used in distinguishing types of anaemia. Recently, it was reported that it could reflect the burden of inflammation in diverse diseases and their prognosis. Hence, in this study, we investigated whether RDW may contribute to discriminating adult onset Still's disease (AOSD) from sepsis in serious febrile patients within 24 hours after hospitalization. Methods: We reviewed the medical records and enrolled 21 AOSD patients, 27 sepsis patients and 30 matched healthy controls. We collected at least two laboratory results of variables including RDW within 24 hours after hospitalization, and we calculated their mean values. Results: Sepsis patients showed the significantly increased median white blood cell count, compared to AOSD patients ($14,390.0/mm^3$ vs. $12,390.0/mm^3$, p = 0.010). The median RDW in sepsis patients was higher than that in AOSD patients (15.0% vs. 13.3%, p = 0.001), and furthermore, the median RDW in both patient-groups was significantly higher than that in healthy controls. In contrast, the median ferritin level in sepsis patients was lower than that in AOSD patients (544.0 mg/dL vs. 3,756.6 mg/dL, p = 0.001). In multivariate analysis, RDW ${\geq}14.8%$ (odds ratio, 17.549) and ferritin < 2,251.0 mg/dL (odds ratio, 32.414) independently suggested sepsis more than AOSD in patients initially presenting with fever requiring hospitalization. Conclusions: RDW might be a rapid and helpful marker for a differential diagnosis between AOSD from sepsis at an early phase.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.3
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• pp.1411-1414
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• 2016

Background: Eosinophilic pleural effusion (EPE) is an eosinophil count more than 10% on cytology of pleural samples. Recently, it was reported that malignancy had been the most prevalent cause inducing EPE. Therefore, we conducted an analysis on the prevalence and etiology of EPE and investigated the relationship between EPE and malignancy. Materials and Methods: Data for pleural cell differential count from patients receiving thoracentesis during the period from January 2008 to December 2013 were compared with clinical data and established diagnosis of patients obtained via electronic chart review. Results: A total of 6,801 requests of pleural cytology from 3,942 patients with pleural effusion who had received thoracentesis were available at Far Eastern Memorial Hospital from 2008 to 2013, and of these subjects, 115 (2.9%) were found to have EPE. The most frequent cause of EPE was malignancy (33.0%, n=38), followed by parapneumonic effusions (27.8%, n=32), tuberculosis pleuritis (13.9%, n=16), transudate effusions (12.2%, n=14) and the presence of blood or air in pleural space (10.4%, n=12). Additionally, an inverse relationship of eosinophilia in pleural fluid was identified in patients with malignancy and EPE. The cut-off eosinophil count in pleural fluid was 15% for the most accurate discrimination between malignancy and benign disorders in patients with EPE. At the cut-off level, the sensitivity and specificity were 65.8% and 67.5%, respectively. Conclusions: Pleural fluid eosinophilia was a speculative negative predictor for malignancy, despite the fact that cancers, including lung cancers and metastatic cancers to lung, were the most leading cause of pleural fluid eosinophilia. An inverse correlation was observed between the pleural eosinophil percentage and the likelihood of malignancy in patients with EPE.

• Korean Journal of Animal Reproduction
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• v.20 no.1
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• pp.27-34
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• 1996

The objective of this study was to examine the cell number of Total, ICM and TE cells of bovine blastocysts according to development progression cultured in CR1 medium, which was reported as successfully supporting medium for preimplantaion bovine embryo development to the blastocyst stage, by differential labelling of the nuclei with immunosurgery and polynucleot-ide-specific fluorochromes. Blastocysts were obtained at day 8 after in vitro fertilization and classified to early, middle, expanded stage according to the developmental morphology; blastocoel expansion and zona thickness. Also, bias tocysts in the same category were divided into two parts to check the Total cell number by using bisbenzimide only and ICM, TE and Total cell number by using immunosurgery and two polynucleotide-specific fluorochromes. 1) The development rate of blastocysts at day 8 after in vitro fertilization was 29.3% and classified bIas tocysts to early, middle, expanded and hatching stage were 8.7, 9.9, 7.6 and 3.1%, respectively. 2) The numbers of total blastomere using bisbenzimide in the classified blastocysts to early, middie and expanded were 46.9${\pm}$8.6, 66.2${\pm}$12.5 and 122.8 ${\pm}$ 14.4, respectively. This indicated that CR1 is a appropriate culture medium for bovine embryo development. 3) The count of ICM and TE cell number by using differential labelling with immunosurgery and polynucleotide-specific fluorochromes in the classified blastocysts to early, middle and expanded; ICM cell numbers of were 12.8${\pm}$5.9, 26.3${\pm}$8.4 and 35.5${\pm}$15.0, respectively and TE cell numbers were 30.5${\pm}$5.0, 4 41.3${\pm}$8.2 and 81.1${\pm}$13.4, respectively. These results presented that the increase of ICM and TE cell numbers averaged two and three doublings between early and expanded blastocyst stage and also total cell number counted from ICM nuclei and TE nuclei by using differential label-ling showed the increase pattern with development advance level and the results were similar to total cell number obtained from bisbenzimide treatment only. Therefore, the differential labelling of ICM and TE nuclei in situ is a very useful technique to evaluate embryo qualities and can be used as an indicator on study of preim-plantation embryo development.

• Clinical and Experimental Reproductive Medicine
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• v.23 no.3
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• pp.319-326
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• 1996

The objective of this study was to investigate correlation between the morphology by microscopic assessments of surplus blastocysts produced in human IVF program and their cell number obtained by differential labelling method. For these experiments, 76 surplus human blastocysts were obtained from 36 patients on day 5 after IVF, the embryos were classified to early (ErB), early expanding (EEB), middle expanding (MEB), expanded blastocyst (EdB) according to their blastocoel expansion and zona thickness. When the ovum size and zona thickness of the classified blastocysts were measured using micrometer, although the embryos were produced in the same culture condition, there were significant variances in ovum size ($148.8 217.6{\mu}m$) and zona thickness ($1.2-14.4{\mu}m$). Total blastomere cell number counted after hoechst staining was increased by two to three fold during the transition period from ErB ($39.1{\pm}3.6$) to EdB ($(89.6{\pm}3.3)$) stage on day 5 after IVF. ICM ($11.9{\pm}1.8-22.2{\pm}4.3$) and TE ($24.5{\pm}3.6-70.0{\pm}7.7$) cell numbers using differential labelling were also showed the increased pattern according to the developmental level. Especially, EdB which showed poor ICM morphologically also indicated the low ICM cell number after differential labelling. This demonstrated that there is good correlation between the morphological assessment and the cell number. The count of ICM and TE nuclei using differential labelling can be used as an important criterion, if it is accompanied with morphological assessments, in selecting the better embryos for improving the pregnancy rates in human blastocyst transfer program.

• Journal of Yeungnam Medical Science
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• v.4 no.2
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• pp.89-96
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• 1987

Using same TDE factors, the authors studied the effects of whole abdominal irradiation on body weight and peripheral blood picture in $30{\pm}3$ day old mice. Fractions of 100 and 200 cGy were given five times a week to the final TDF factors l7, 33, and 49. Total 80 mice were irradiated with orthovoltage x-ray machine. Our results can be summarized as follows : There were no significant differences by sex. Body weight was progressively decreased by the duration for irradiation, but no remarkable difference by fractional dose. Hemoglobin level showed no remarkble change by fractional dose or TDF factors. Leukopenic changes showed that severity of decrease seems to be more related to TDF factors. There were no significant changes of differential count by TDF factors of total doses.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.11
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• pp.1599-1603
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• 2003

The physical feature, physiological character and behavior studies were conducted with fifteen newly collected gayals in Bandarban hill tract area of Bangladesh. Their morphology is different from domestic cattle. The range of pulse rate, body temperature and respiration rate were 47 to 75 per minute, 37.78 to $38.88^{\circ}C$ and 20 to 40 per minute, respectively. These physiological values vary with different age group and seasonal variation. In hematological feature, the average findings were RBC $7.01{\pm}0.52$ million/cu.mm, WBC $14.3{\pm}3.69$ thousand/cu.mm, hemoglobin concentration $9.81{\pm}2.25gm%$, PCV $35.86{\pm}3.68%$. In differential WBC count neutrophils $28.23{\pm}1.75%$, lymphocytes $62{\pm}2.05%$, monocytes $4.4{\pm}1.34%$, eosinophils $5{\pm}2.49%$ and basophils $0.4{\pm}0.51%$. In behavior study, the animal shows browsing nature on hill slopes. They are watchful in new environment, become excited and nervous with strangers. Heated female gayals response for mating with domestic bull.

• Journal of the Korean Society of Clothing and Textiles
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• v.39 no.5
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• pp.778-788
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• 2015

This study evaluated the characteristics and sensibility of work clothes materials developed that took into account the work environment of major industrial settings, for which a selection was made according to spring/autumn use and winter use. The physical properties of the existing and test weaving of the work clothes materials were compared after the test weaving of work clothes materials through material design, which altered the yarn count, composition, weave and density of material. To evaluate sensibility of work clothes materials subjectively, seven ranks' semantic differential scale questions were developed with polar adjective pairs. The test weaving of work clothes materials showed improved performance (such as tensile strength, dimensional change, water vapor permeability, and color fastness) compared to the existing materials. The common factor analysis indicated that the explanatory power of the cumulative variance for the spring/autumn use and winter use was 71.19% and 69.53%, respectively.

• Journal of the Korean Institute of Telematics and Electronics
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• v.26 no.11
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• pp.1666-1671
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• 1989

A novel switched-capacitor circuit for interfacing capacitive microtransducers with a digital system is developed based on the dual-slope integration. It consists of a differential integrator and a comparator. Driven by the teo phase clock, the circuit first senses the capacitance difference between the transducer and the reference capacitor in the form of charge, and accumulates it into the feedback capabitor of the integrator for a fixed period of time. The resulant accumulated charge is next extracted by the known reference charge until the integrator output voltage refurns to zero. The length of time required for the integrator output to return to zero, as measured by the number of clock cycle gated into a counter is proportional to the capacitance difference, averaged over the integration period. The whole operation is insensitive to the reference voltage and the capacitor values involved in the circuit, Thus the proposed circuit permits an accurate differental capacitance measurement. An error analysis has showh that the resolution as high as 8 bits can be expected by realizing the circuit in a monolithic MOS IC form. Besides the accuracy, it features the small device count integrable onto a small chip area. The circuit is thus particularly suitadble for the on-chip interface.

• Clinical and Experimental Pediatrics
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• v.51 no.8
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• pp.792-796
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• 2008

Bone pain in a child could be associated with cancer as an initial manifestation of the disease. The childhood malignancies that frequently present bone pain are leukemia, neuroblastoma, and primary bone tumors such as osteosarcoma and Ewing sarcoma. Persistent bone or joint pain associated with swelling, mass, or limitation of motion implies underlying serious causes. Systemic manifestations such as lymphadenopathy, hepatosplenomegaly, fever, fatigue, night sweat, and laboratory abnormalities are also suggestive of malignancy. The index of suspicion tends to be low since less than 1% of children who complain of bone pain are diagnosed as cancer. Nonetheless, pediatricians should be alert to the possibilities of cancer since early detection and prompt treatment might reduce mortality.

• Korean Journal of Veterinary Research
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• v.16 no.2
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• pp.179-185
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• 1976

The gross appearance, cellular constituents, and quality of mucin clot of synovial fluid from the normal tibiotarsal joints of 57 healthy Korean cattle were investigated. The results obtained were summarized as followings. 1. The gross appearance of the synovial fluid were quite clear without any minute particles of flocculent, colourless (78.95%) or slightly yellowish (21.05%), and it did not coagulate within the 24 hours after sampling. 2. The mean number of leucocytes for the entire group was $127.68{\pm}13.84/mm^3$ (SE) with a range of 0 to $627/mm^3$ for individual samples. In the total cell count no significance among the investigated age and sex categories was found. 3. The mean differential values for the groups ware: neutrophil, $9.36{\pm}1.67%$; lymphocyte, $57.66{\pm}8.34%$; monocyte, $24.70{\pm}2.22%$; eosinophil, $1.45{\pm}0.27%$; and macrophage, $5.83{\pm}1.13%$. No basophils were observed in stained smears of synovial fluid. 4. Normal mucin clot quality was recorded for 94.7% of the animals, but 5.3% were observed to have fair mucin clot quality. Quality of mucin clot was approximately equal for bulls, cows, and calves.