• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2002년도 추계학술대회
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• pp.15-21
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• 2002

Terminal-restriction fragment length polymorphism (T-RFLP) analysis has been optimized by using in vitro model community composed of genomic DNAs of known bacterial strains and has been applied to assess the bacterial community structure in marine sediments. The specific fluorescence-labeled terminal restriction fragments (T-RFs) between 39 and 839 base long specifying each strain were precisely measured for known bacterial strains. The addition of a co-solvent (dimethylsulfoxide or glycerol) into PCR reactions has reduced differential PCR amplification. Comparative bacterial community structure was investigated for pristine and polluted sediments. A complex T-RFLP pattern showing complex bacterial community structure was obtained in the pristine sediment, whereas simple T-RFLP pattern (low bacterial diversity) was shown in polluted sediments where caged aquaculture has been conducted for several years. The results of T-RFLP analysis were compared with that of cloning and sequencing 16S rDNA clones from the same sediments. Sequence analysis of 16S rDNA clones (72) of the pristine sediment revealed a diverse collection of lineages, largely of the class Proteobacteria ($6\%$ alpha subdivision, $46\%$ gamma subdivision, $13\%$ delta subdivision, and $3\%$ epsilon subdivision), Nitrospina $(8\%)$, high G+C gram positive $(8\%)$, Verrucomicrobia $(7\%)$, and Planctomycetes $(6\%)$. In the contaminated sediments, 17 $(59\%)$ of the 16S rDNA clones (29) were related to Campylobacter and symbiont of Rimicaris exoculata belonging to epsilon subdivision of Proteobacteria. The results obtained indicated that T-RFLP analysis is a rapid and precise technique for comparative bacterial community analysis.

• Parasites, Hosts and Diseases
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• 제46권3호
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• pp.157-164
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• 2008

Three Acanthamoeba isolates (KA/E9, KA/E17, and KA/E23) from patients with keratitis were identified as Acanthamoeba triangularis by analysis of their molecular characteristics, a species not previously recognized to be a corneal pathogen. Epidemiologic significance of A. triangularis as a keratopathogen in Korea has been discussed. Morphologic features of Acanthamoeba cysts were examined under a microscope with differential interference contrast (DIC) optics. Mitochondrial DNA (mtDNA) of the ocular isolates KA/E9, KA/E17, and KA/E23 were digested with restriction enzymes, and the restriction patterns were compared with those of reference strains. Complete nuclear 188 and mitochondrial (mt) 16S rDNA sequences were subjected to phylogenetic analysis and species identification. mtDNA RFLP of 3 isolates showed very similar patterns to those of SH621, the type strain of A. triangularis. 16S and 18S rDNA sequence analysis confirmed 3 isolates to be A. triangularis. 18S rDNA sequence differences of the isolates were 1.3% to 1.6% and those of 16S rDNA, 0.4% to 0.9% from A. triangularis SH621. To the best of our knowledge, this is the first report, confirmed by 18S and 16S rDNA sequence analysis, of keratitis caused by A. triangularis of which the type strain was isolated from human feces. Six isolates of A. triangularis had been reported from contaminated contact lens cases in southeastern Korea.

• Steel and Composite Structures
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• 제45권5호
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• pp.729-747
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• 2022

The critical buckling loads and buckling modes of bi-directional functionally graded porous unified higher order shear plate with elastic foundation are investigated. A mathematical model based on neutral axis rather than midplane is developed in comprehensive way for the first time in this article. The material constituents form ceramic and metal are graded through thickness and axial direction by the power function distribution. The voids and cavities inside the material are proposed by three different porosity models through the thickness of plate. The constitutive parameters and force resultants are evaluated relative to the neutral axis. Unified higher order shear plate theories are used to satisfy the zero-shear strain/stress at the top and bottom surfaces. The governing equilibrium equations of bi-directional functionally graded porous unified plate (BDFGPUP) are derived by Hamilton's principle. The equilibrium equations in the form of coupled variable coefficients partial differential equations is solved by using numerical differential integral quadrature method (DIQM). The validation of the present model is presented and compared with previous works for bucking. Deviation in buckling loads for both mid-plane and neutral plane are developed and discussed. The numerical results prove that the shear functions, distribution indices, boundary conditions, elastic foundation and porosity type have significant influence on buckling stability of BDFGPUP. The current mathematical model may be used in design and analysis of BDFGPU used in nuclear, mechanical, aerospace, and naval application.

• Structural Engineering and Mechanics
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• 제90권3호
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• pp.219-232
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• 2024

In current study, for the first time, Nonlinear Bending of a skew microplate made of a laminated composite strengthened with graphene nanosheets is investigated. A mixture of mechanical and thermal stresses is applied to the plate, and the reaction is analyzed using the First Shear Deformation Theory (FSDT). Since different percentages of graphene sheets are included in the multilayer structure of the composite, the characteristics of the composite are functionally graded throughout its thickness. Halpin-Tsai models are used to characterize mechanical qualities, whereas Schapery models are used to characterize thermal properties. The microplate's non-linear strain is first calculated by calculating the plate shear deformation and using the Green-Lagrange tensor and von Karman assumptions. Then the elements of the Couple and Cauchy stress tensors using the Modified Coupled Stress Theory (MCST) are derived. Next, using the Hamilton Principle, the microplate's governing equations and associated boundary conditions are calculated. The nonlinear differential equations are linearized by utilizing auxiliary variables in the nonlinear solution by applying the Frechet approach. The linearized equations are rectified via an iterative loop to precisely solve the problem. For this, the Differential Quadrature Method (DQM) is utilized, and the outcomes are shown for the basic support boundary condition. To ascertain the maximum values of microplate deflection for a range of circumstances-such as skew angles, volume fractions, configurations, temperatures, and length scales-a parametric analysis is carried out. To shed light on how the microplate behaves in these various circumstances, the resulting results are analyzed.

• 한국지반공학회지:지반
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• 제14권6호
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• pp.93-112
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• 1998

본 연구에서는, 연약한 준설매립점토지반 내부에 플라스틱 배수재를 수평으로 설치하여 압밀침하의 가속화를 유도하는 수평배수공법의 해석절차 체계화에 초점을 두고 이론적 및 실험적 접근을 진행하였다. 이를 위해, 유한변형률 개념을 근거로 한 Gibson등의 1차원 자중압밀이론을 토대로, 자중압밀효과 및 수평배수재 설치에 따른 추가 압밀효과를 복합적으로 고려하는 지배방정식을 제시하였으며, 본 지배방정식 및 수평배수재 설치효과를 고려한 초기조건 및 경계조건 등을 토대로, Dufort-Erankel의 유한차분화 알고리즘을 이용해 시간경과에 따른 침하량 및 압밀도 등의 변화를 예측하기 위한 해석절차를 제시하였다. 또한 제시된 해석절차의 적용 타당성 확인을 위해, 안산 시화지구 준설매립점토 및 플라스틱 수평배수재를 이용한 실내모형실험을 실시하였다. 실내실험을 통해, 안산 시화지구 준설매립점토의 간극비-유효응력 관계 및 투수계수-간극비 관계 등을 정량적으로 정의하기 위한 기초자료를 획득 하였으며. 또한 시간경과에 따른 깊이별 침하량등을 측정하여 본 연구에서 제시된 해석절차에 의한 예측치와 비교.분석하였다. 이외에도 플라스틱 배수재 수평배수공법에 관련된 설계변수가 압밀침하등에 미치는 영향을 제시된 해석절차를 이용해 분석하였으며, 이 결과를 토대로 한 설계도표의 제시도 이루어 졌다.

• 콘크리트학회논문집
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• 제22권2호
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• pp.159-169
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• 2010

경량골재 사전흡수에 의한 콘크리트 내 수분이동 변화와 수축 저감 효과를 파악하기 위하여 일반콘크리트 및 경량콘크리트의 수분이동, 열전달, 변형률의 다중물리모델의 해석과 실험을 수행하였다. 그 결과 동일 물-결합재비일 경우 경량 콘크리트와 일반 콘크리트와의 습도 변화 비교는 모두 경량 콘크리트가 일반 콘크리트보다 초기재령, 장기 재령 모두에서 작은 습도 감소를 나타내어 경량 골재 사전흡수수에 의한 수분 공급이 효과적으로 이루어진 것으로 판단된다. 이에 따라 경량 콘크리트는 일반 콘크리트에 비해 수축변형률 크기 및 분포 모두 감소하였으며, 수축저감효과는 물-시멘트비 0.3에서는 초기재령에서, 물-시멘트비 0.5에서는 초기재령, 장기재령 모두에서 효과적인 것으로 나타났다. 수분이동 및 변형률 해석과 모형실험을 통해 일련된 연구에서 도출한 수분이동 특성값과 습도, 수축 관련식은 적합한 것으로 판단되며, 향후 경량콘크리트의 부등수축해석에 적절히 활용될 수 있을 것으로 생각된다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권3호
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• pp.248-253
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• 2005

AfsR2, originally identified from Streptomyces lividans, is a global regulatory protein which stimulates antibiotic biosynthesis. Through its stable chromosomal integration, the high level of gene expression of afsR2 significantly induced antibiotic production as well as the sporulation of S. lividans, implying the presence of yet-uncharacterized AfsR2-target proteins. To identify and evaluate the putative AfsR2-target proteins involved in antibiotic regulation, the proteomics-driven approach was applied to the wild-type S. lividans and the afsR2-integrated actinorhodin overproducing strain. The 20 gel-electrophoresis gave approximately 340 protein spots showing different protein expression patterns between these two S. lividans strains. Further MALDI-TOF analysis revealed several AfsR2-target proteins, including glyceraldehyde-3-phosphate dehydrogenase, putative phosphate transport system regulator, guanosine penta phosphate synthetase/polyribonucleotide nucleotidyltransferase, and superoxide dismutase, which suggests that the AfsR2 should be a pleiotropic regulatory protein which controls differential expressions of various kinds of genes in Streptomyces species.

• Journal of Welding and Joining
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• 제33권5호
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• pp.31-34
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• 2015

Microstructure and tensile property in the weld heat-affected zone (HAZ) of austenitic Fe-Mn-Al-C low density steels were investigated through transmission electron microscopy analysis and tensile tests. The HAZ samples were prepared using Gleeble simulation with high heat input welding condition of 300 kJ/cm, and the HAZ peak temperature of $1200^{\circ}C$ was determined from differential scanning calorimetry (DSC) test. The strain- stress responses of base steels showed that the addition of V improved the tensile and yield strength by grain refinement and precipitation strengthening. Tensile strength and elongation decreased in the weld HAZ as compared to the base steel, due to grain growth, while V-added steel had a higher HAZ strength as compared than V-free steel.

• Earthquakes and Structures
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• 제15권3호
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• pp.285-294
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• 2018

In this study, nonlinear dynamic response of a concrete plate retrofit with Aluminium oxide ($Al_2O_3$) under seismic load and magnetic field is investigated. The plate is a composite reinforced by Aluminium oxide with characteristics of the equivalent composite being determined using Mori-Tanka model considering agglomeration effect. The plate is simulated with higher order shear deformation plate model. Employing nonlinear strains-displacements, stress-strain, the energy equations of column was obtained and using Hamilton's principal, the governing equations were derived. Differential quadrature method (DQM) in conjunction with Newark method is applied for obtaining the dynamic response of structure. The influences of magnetic field, volume percent of nanoparticles, geometrical parameters of column, agglomeration and boundary conditions on the dynamic response were investigated. Results showed that with increasing volume percent of nanoparticles, the dynamic deflection decreases.

• 한국미생물·생명공학회지
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• 제22권4호
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• pp.368-372
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• 1994

Three more unusual macrolides in addition to concnamycin B were isolated from the mycelium of Streptomyces sp. strain Bal6. These four compounds showed a potent cytotoxity to hunian cancer cell lines, SNU-1 (stomach cancer cell line), SNU-354 (liver cancer cell line), MCF- 7 (breast cancer cell line) and KB-3-1 (oral epidermoid carcinoma cell line). Interestingly, these compounds confered slight differential cytotoxity on RHEK-1, a human epidermal keratinocyte cell line immotalized by AD12-SV40 hybrid virus and RHEK-1/pSV$_{2}$ ras which was resulted from H-ras transfomation of RHEK-1. These compounds were determined to be concanamycin A, conca- namycin E and 0-methyl concanamycin B by NMR and other spectral analysis.