• Journal of Positioning, Navigation, and Timing
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• v.5 no.1
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• pp.21-28
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• 2016

The Wide Area Differential Global Positioning System (WADGPS) that uses a number of Global Navigation Satellite System (GNSS) reference stations are implemented with various types and provide services as it can service a wide range of areas relatively. This paper discusses a constellation design of reference stations and performance analysis of the WADGPS. It presented performance results of static and dynamic users when wide area correction algorithm was applied using eight reference stations.

• Korean Journal of Veterinary Service
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• v.46 no.1
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• pp.15-27
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• 2023

Bordetella (B.) bronchiseptica, Mycoplasma (M.) cynos, and M. canis are the major bacterial pathogens that cause canine infectious respiratory disease complex (CIRDC). In this study, we developed a triplex real-time polymerase chain reaction (tqPCR) assay for the differential detection of these bacteria in a single reaction. The assay specifically amplified three bacterial genes with a detection limit of below 10 copies/reaction. The assay showed high repeatability and reproducibility, with coefficients of intra- and inter-assay variations of less than 1%. The diagnostic results of the assay using 94 clinical samples from household dogs with CIRDC clinical signs, the prevalence of B. bronchiseptica, M. cynos, and M. canis was 22.3%, 18.1%, and 20.2%, respectively, indicating that the diagnostic sensitivity was comparable to those of previously reported qPCR assays. The dual infection rate of B. bronchiseptica and M. cynos, B. bronchiseptica and M. canis, and M. cynos and M. canis was 5.3%, 7.4%, and 3.1%, respectively. Moreover, the triple infection rate of B. bronchiseptica, M. cynos, and M. canis was 2.1%. These results indicate that coinfections with B. bronchiseptica, M. cynos, and M. canis have frequently occurred in the Korean dog population. The newly developed tqPCR assay in the present study will be a useful tool for etiological and epidemiological studies on these three CIRDC-associated bacterial pathogens. The prevalence and coinfection data revealed through this study will contribute to expanding knowledge on the epidemiology of CIRDC in the recent Korean dog population.

• Horticultural Science & Technology
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• v.31 no.5
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• pp.598-606
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• 2013

Anther is the major organ of flower in responsible to reproduction and outward appearance. From anther-specific cDNA library of Lilium Oriental Hybrid 'Acapulco', 2000 expressed sequence tags were selected randomly. Differential slot blot analysis with cDNA probes from the anther and leaf was used to get anther-expressed clone and 570 non-redundant ESTs were obtained and sequenced. Compared to the GenBank database using BLASTX algorithm, 191 clones showed significant similarity but others (66.5%) did not measured to known sequence. Functional categories according to gene ontology (GO) annotation included sequence representing a significant portion of protein in cell and cell part respectively. A transcriptional analysis at 7 different organs and developmental stage was performed using northern blot with thirty ESTs as putative anther specific gene. This report suggest that selection of anther expressed clone using differential slot blot was considered as very effective tool and our current study can provide fundamental information on the lily anther including pollen furthermore.

• Journal of Agricultural Extension & Community Development
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• v.8 no.1
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• pp.15-23
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• 2001

As Internet and World Wide Web become more popular in our society, the interests in effective application to the field of rural development and agricultural extension service are increasing. The potential in Web-based agricultural extension service is expressed in many areas, such as agriculture information, Web-based training and education, and virtual rural community for rural development and agricultural extension service. The research on the Web and its related issues has been conducted in many ways, but the interests in the competencies and skills of staff who are in charge of Web developing and managing have not been exposed yet. The purpose of this study was to investigate the important competencies and skills for Web developing and managing for enhancing the effectiveness of rural development and agricultural extension service. Twelve extension service staff and on staff in the extension service support center were interviewed. The results of study showed that there were eight essential competencies; competencies to understand rural community and agricultural extension service, to understand the farmers as Web users, to design and develop effective Web sites, to understand the nature of hypertext, to utilize instruction theories, to absorb new technology, to manage projects, and to utilize external sources. The results of study also showed that there was lack of support systems for Web development and information. Overall, the findings of this study suggest that establishing long-term education and training system for technology, electronic performance support systems, and cooperation with universities and colleges for technology are of particular importance for promoting Web-based extension services.

• Convergence Security Journal
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• v.8 no.4
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• pp.41-49
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• 2008

VoIP is a service that changes the analogue audio signal into a digital signal and then transfers the audio information to the users after configuring it as a packet; and it has an advantage of lower price than the existing voice call service and better extensibility. However, VoIP service has a system structure that, compared to the existing PSTN (Public Switched Telephone Network), has poor call quality and is vulnerable in the security aspect. To make up these problems, TLS service was introduced to enhance the security. In practical system, however, since QoS problem occurs, it is necessary to develop the VoIP security system that can satisfy QoS at the same time in the security aspect. In this paper, a user authentication VoIP system that can provide a service according to the security and the user through providing a differential service according to the approach of the users by adding AA server at the step of configuring the existing VoIP session is suggested. It was found that the proposed system of this study provides a quicker QoS than the TLS-added system at a similar level of security. Also, it is able to provide a variety of additional services by the different users.

• Korean Journal of Veterinary Service
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• v.30 no.4
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• pp.489-496
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• 2007

An oligonucleotide microarray system was developed for the simultaneous detection of porcine epidemic diarrhea virus, transmissible gastroenteritis virus, porcine enteric calicivirus, porcine group A and C rotavirus. RNAs of the reference viruses and porcine diarrhea samples were extracted and amplified using one-step multiplex RT-PCR in the presence of cyanine 5-dCTP and hybridized on the microarray chip that spotted the virus-specific oligonucleotides. This system were approximately 10-to 100-fold higher in sensitivity than conventional RT-PCR, and the assay time was less than 3 hours. The relative sensitivity and specificity were 92% and 72.2%, respectively, based on 102 porcine diarrhea samples using RT-PCR as gold standard. These results suggested that the oligonucleotide microarray system in this study be probably more reliable and reproducible means for detecting porcine enteric viruses and that it could be of substantial use in routine diagnostic laboratories.

• Korean Journal of Veterinary Service
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• v.31 no.4
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• pp.485-492
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• 2008

Salmonella enterica serovars Gallinarum(SG, causative agent of fowl typhoid) and S Pullorum(SP, causative agent of pullorum disease) are very important bacterial pathogens in poultry industry. They share some common antigenic properties though the characteristics of outbreaks are quite different. To discriminate between SG and SP, we developed two rapid diagnostic methods, allele-specific real-time PCR and 3'-tailed PCR over 2 single nucleotide polymorphisms ($237^{th}\;and\;598^{th}$). In both methods, $237^{th}$ allele was found to be a good target for differential diagnosis, while $598^{th}$ allele produced some non-specific reactions.

• Journal of Korean Library and Information Science Society
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• v.40 no.1
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• pp.93-113
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• 2009

A special library using Web 2.0 technologies should explore a new service to meet appropriately the needs for users. By applying the RSS and OPEN API, this paper developed a new information service for the Korea Institute of Public Finance(KIPF). In order for the library to sustain in the future challenges, it should participate actively in the developments of new strategic options and technological changes.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.21 no.2
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• pp.417-426
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• 1996

The end-to-end service clock recovery is a critical issue in providing constandt bit rate service through ATM network. The Synchronous Residual Time Stamp(SRTS) algorithm is used to recovery the source clock using time stamp of transmitter. In thispaper, we propose a Differential Residual Time Stamp (DRTS) transmission mechanism to effectively deliver the timing information of source clock in SRTS algorithm. The RTS transmission method simple in its hardware. From the results of field trial of DS3 rate interactive video communication system through B-ISDN testbed, it can be identified that DRTS method is superior to the RTS method.

• Journal of information and communication convergence engineering
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• v.10 no.3
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• pp.225-230
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• 2012

The Korean nationwide differential global positioning system (NDGPS) reference station transmits a global positioning system (GPS) enhancement signal using minimum shift keying modulation with a 200 bps data rate. The ocean-based DGPS covers the service area of 100 NM with 300 W output power; on the other hand, the land-based DGPS transmits the output power of 500 W, which covers the service area of 100 km. The DGPS reference stations with high output power can radiate spurious signals, which may act as interference sources affecting the other DGPS reference stations or the wireless ground stations that utilize the medium frequency band. In this paper, the radiation spectrums of the DGPS reference stations are measured and analyzed in the spurious domain. The DGPS radiation spectrums are evaluated from the perspective of the interference effect.