• 한국작물학회지
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• 제51권spc1호
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• pp.174-178
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• 2006

본실험은 조생종인 하대두형 및 중국수집종 콩과 중숙${\sim}$만숙종의 국내 육성 종과의 isoflavone 함량변이를 비교하며, 파종기 이동 및 콩의 생육단계별 생육기간에 따른 isoflavone함량의 차이를 분석하고자 수행하였으며, 결과를 요약하면 다음과 같다. 1. 전체 isoflavone함량은 파종기 이동에 관련 없이 국내 수집종이 하대두형 및 중국수집 종에 비하여 놓았으며, 하대 두형콩과 중국수집 종과 비슷한 함량 분포를 나타냈다. 2. 파종기이동에 따른 총 isoflavone 함량은 파종기를 늦게 할수록 하대두형, 중국수집종 및 국내육성종 모두에서 증가하는 경향 이었다. 3. 콩의 개화기${\sim}$성숙기의 기간 및 생육일수가 길어질수록 isoflavone 함량도 높아지는 경향을 나타냈으며, 통계적으로도 부합되었다. 4. 콩의 isoflavone 함량은 입중이 무거울수록 함량이 낮아 졌으며, 상관계수는 4월${\sim}$5월 파종구에서 각각 -0.57, -0.51 및 -0.38로 분석되었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.100-100
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• 2017

FT is one of the major floral activator in plant flowering. FT determines the changing point of time from vegetative stage to reproductive stage. To understand the role of FT homologs in short-day plant soybean, we identified 10 soybean FT homologous genes and named GmFTs. We figured out that 10 GmFT genes were further categorized into three subclades through phylogenetic analysis. Expression analysis of GmFT genes indicated that they might have different functions in photoperiod-dependent soybean flowering. Most of GmFTs, for example, GmFT2a, GmFT2b, GmFT5a and GmFT6 mainly expressed in soybean leaves at short-day condition. However, interestingly GmFT1a and GmFT4 represented opposite expression pattern to other GmFTs. Arabidopsis transgenic plants overexpressing GmFT2a and GmFT5a exhibited extremely early flowering. In contrast, overexpression of GmFT4 delayed flowering of Arabidopsis transgenic plants. The results suggest that GmFT4 has antagonistic role to other GmFTs in soybean flowering. Interestingly, mRNA level of GmFT2a is higher in early flowering soybean accessions than in late flowering ones. Moreover, the highest point of mRNA level of GmFT2a showed the positive correlation with the timing of flowering of soybean accessions. But that of GmFT4 showed opposite pattern. Here, we report that soybean FT homologs might acquire different functions in photoperiod-dependent flowering through the functional diversification during evolution.

• The Plant Pathology Journal
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• 제39권6호
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• pp.575-583
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• 2023

Fusarium root rot is an increasingly severe problem in soybean cultivation. Although several Fusarium species have been reported to infect soybean roots in Heilongjiang province, their frequency and aggressiveness have not been systematically quantified in the region. This study aimed to investigate the diversity and distribution of Fusarium species that cause soybean root rot in Heilongjiang province over two years. A total of 485 isolates belonging to nine Fusarium species were identified, with F. oxysporum and F. solani being the most prevalent. Pot experiments were conducted to examine the relative aggressiveness of different Fusarium species on soybean roots, revealing that F. oxysporum and F. solani were the most aggressive pathogens, causing the most severe root rot symptoms. The study also assessed the susceptibility of different soybean cultivars to Fusarium root rot caused by F. oxysporum and F. solani. The results indicated that the soybean cultivar DN51 exhibited the most resistance to both pathogens, indicating that it may possess genetic traits that make it less susceptible to Fusarium root rot. These findings provide valuable insights into the diversity and distribution of Fusarium species that cause soybean root rot and could facilitate the development of effective management strategies for this disease.

• 한국작물학회지
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• 제45권2호
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• pp.143-149
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• 2000

From the soils of soybean fields in Cotton Branch Station (CBS) and Pine Tree Station (PTS), Arkansas, USA, various single spore isloates of sudden death syndrome (SDS) pathogen were obtained on modified Nash ＆ Snyder's medium (MNSM) with dilution plating technique and transferred to potato dextrose agar (PDA) medium to identify the cultural colony shape. The colony shapes of these isolates resembled F. solani isolate 171 which was white and chalky shaped on MNSM and most of them had unique form of morphology which produced white margin and blue center colony on PDA. Although, some of these isolates had more dark blue or showed slightly different color, all isolates that were selected randomly for green-house inoculation assay produced typical foliar symptoms on leaves of soybean, Hartz 6686. To determine the genetic differences among the isolates, mitochondrial DNA restriction fragment length polymorphism (RFLP) was conducted with fourty isolates from both fields, using mtDNA probes, 2U18 and 4U40, derived from Colletotrichum orbiculare. We obtained distinctive RFLPs in each treatment of restriction enzyme, EcoRI and HaeⅢ. Isolates, 11-2-5 and 14-3-1-1, from CBS and isolates, 104-3-1-2 and 701-1-5-1, from PTS showed different band patterns from 171 in both or in either treatment of restriction enzymes. Even if some of these isolates showed heterogeneous, they were more closer to 171 than PN603. And, also, rest of the thirty-six isolates had exactly same polymorphisms as 171 in each treatment of restriction enzyme. Although, some of the isolates showed the different morphological shape on PDA and slightly different band patterns on RFLPs, all of the isolates selected on MNSM due to their distinctive colony shape from other fungi produced the typical foliar symptoms on soybean leaves in greenhouse inoculation assay. It might be suggested that these isolates were not genetically different from check isolate 171 and they were unique strain of F. solani.

• 한국초지조사료학회지
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• 제37권2호
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• pp.101-107
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• 2017

Corn is basal forage for livestock species in Republic of Korea but it lacks protein and needs nitrogenous fertilizer. This study was designed with main objective to achieve optimum growth, yield & nutritive value of forage for livestock through implementing corn-soybean intercropping strategy at 17 different places under Korean condition. Two treatments; corn as monocrop (control treatment) and corn-soybean intercrop were compared under Randomized Block Design from $28^{th}$ May to $8^{th}$ October, 2015. Each treatment had three replicates in each block, whereas seventeen different places were considered as blocks. Data were analyzed through SAS-9.1.3 software. Difference between two treatment means was tested through T-test. Findings depicted that intercropping pattern could not influence (P>0.05) corn plant & ear height, corn lodged stalk No. and corn stalks number. However, corn-soybean intercropping enhanced (P<0.05) forage productivity in terms of total fresh yield ($16.4{\pm}0.7^b$ vs. $19.9{\pm}0.7^atons\;ha^{-1}$), total dry matter yield ($5.38{\pm}0.25^b$ vs. $6.41{\pm}0.31^atons\;ha^{-1}$) and total digestible nutrients yield ($3.94{\pm}0.17^b$ vs. $4.59{\pm}0.21^atons\;ha^{-1}$). Dry matter percentage in corn stalks and corn ears was not different (P>0.05) between two treatments. It was concluded that corn-soybean intercropping strategy was promising technique in enhancing forage productivity though positive symbiotic relation between two crops.

• 한국작물학회지
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• 제36권3호
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• pp.220-225
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• 1991

콩을 연작해온 밭토양에 경명을 재배하므로써 토양선충밀도를 줄일 수 있으며 이것이 콩생육에 어떤 영향을 미치는가를 알기 위하여 콩단작구, 결명단작구, 콩-결명혼작구 및 간작구를 만들어 1년간 재배한 후 그 자리에 3년동안 콩을 재배하면서 선충밀도와 콩의 생육을 조사하엿다 1. 콩단작구에 비하여 결명혼작구 및 간작구에서 토양선충밀도는 크게 낮았고 결명단작구에서는 결명의 생육이 왕성해지면서 토양선충이 완전히 없어 졌다. 2. 결명단작구에 이듬해부터 콩을 계속 재배했어도 토양선충의 밀도는 아주 낮았고 결명혼작고 또는 간작구에 콩을 계속 재배하여도 3년차까지는 콩연작구보다 선충밀도가 훨씬 낮았다. 3. 결명의 뿌리에는 뿌리혹이 착생하지 않는것이 특징인데 콩과 결명을 혼작 또는 간작하여도 콩뿌리에 형성된 뿌리혹의 수는 콩단작구와 차이가 없었다. 4. 콩 성열기의 주당입수, 주당입수 및 주당입중은 결명과 혼작 또는 간작하여도 콩단작구와 차이가 없었다. 결명단작구, 혼작구 및 간작구의 뒷자리에 이듬해부터 콩을 재배한 결과 2년차까지는 콩연작구보다 생육이 더 좋았다.

• Journal of Nutrition and Health
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• 제32권1호
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• pp.110-117
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• 1999

Free radicals formed during the metabolism of environmental chemicals are known to induce mutagenicity, while different types of antioxidants suppress this event. The purpose of this study was to determine the antioxidative and antimutagenic effects of soybean saponins, and to examine the relationship between these two effects for the elucidation of mechanisms involved in the anticarcinogenicity of soybean saponins. Also, antioxidative and antimutagenic effects of soybean saponins were compared with those of kinown antioxidants. For the measurement of antioxidative capacity, soybean saponins, L-ascorbic acid, $\alpha$-tocophoerol, and BHT at concentrations between 005 and 1.0mg/ml were tested for their ability to donate hydrogens and to reduce the formation of thiobarbituric substances(TBARS). Antimutagenic activity was examined using the Ames salmonella test system at concentrations of 600, 900 or 1200ug/ml. Study results showed soybean saponins and all of the other antioxidants tested possessed dose-dependent antioxidative activities. The ability of hydrogen-donation to DPPH was in the order of L-ascorbic acid>$\alpha$-tocopherol=>BHT>soybean saponins. TBARS formation was also inhibited by these compounds, in the order of BHT>$\alpha$-tocopherol=L-ascorbic acid>soybean saponins. Soybean saponins and other antioxidants also showed antimutagenicity in a dose-dependent manner. Especially, soybean saponins and BHT were excellent antioxidants compounds, inhibiting near 80% of the mutagenic effects at a concentration of 1200ug/ml. The correlation coefficients between antioxidative capacity and antimutagenicity for each compund was statistically significant at p<0.05. These results indicate that soybean saponins possess antioxidative and antimutagenic capacities. Also, antimutagenicity of saponins and other antioxidats is partly due to their antioxidative activities.

• 한국식품영양과학회지
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• 제26권3호
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• pp.371-375
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• 1997

The content of genistein and daidzein which were known to be major antioxidative compounds in soybeans were detected by $C_{18}$ reverse phase HPLC. Most of isoflavones in soybeans were detected in the methanol extract but much less amount of isoflavones in the water extract. Among the four different kind of soybeans, the isoflavone content was highest in brown soybean, followed by yellow, small black and black soybean, in the order. These isoflavones were known to be soluble in hot water, which means transfer of isoflavone content was highest in brown soybean, followed by yellow, small black and black soybean, in the order. These isoflavones were known to be soluble in hot water, which means transfer of isoflavones in soy curd into whey during soy curd processing. To identify the change of isoflavone content during processing of soybean curd, soybean curd were made from yellow, brown and black soybean and isoflavone content were determined in each soybean curd, curd residue and whey. Most of soflavones were remained in the whey, it means most of useful antioxidative compounds were wasted. Thus, it is necessary to develop new technology to collect these isoflavones lost during soybean curd processing.

• 동아시아식생활학회지
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• 제20권6호
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• pp.909-915
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• 2010

In order to determine the most effective ratio of black soybean powder as an ingredient in cake, black soybean power was added at ratio of 10, 20, 30, and 40% versus wheat flour set as 0(control). Black soybean powder consisted of moisture(8.44%), crude protein(31.34%), crude fat(6.64%), crude ash(3.88%) and carbohydrates(49.70%). The specific gravity, spreadability, and baking loss increased according to the amount of black soybean powder, although specific volume decreased. The chromaticity 'L' and 'b' values of sponge cake with black soybean powder showed an reducing trend while the 'a' value displayed an increasing trend with an increase in black soybean powder. The texture properties of sponge cake with added black soybean powder showed an increasing trend in hardness and stiffness when the cake contained more black soybean powder. The sensory test for sponge cake with added black soybean powder showed high preference for 20% added black soybean powder to sponge cake.

• 농업과학연구
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• 제46권4호
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• pp.971-980
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• 2019

We profiled the health-promoting bioactive components in nine types of soybean seeds with different seed coat colors (yellow, green, brown, and black) and investigated the effects of different extraction solvents (methanol, ethanol, and water) on their antioxidant activities. The carotenoid and anthocyanin compositions varied greatly by seed color, and the phenolic acids, total phenol, and total flavonoid contents differed by genotype. The carotenoid content was relatively higher in soybean seeds with green and black seed coats than in those with a yellow seed coat while lutein was the most plentiful. The anthocyanin content was considerably higher in the soybean seed with the black seed coat. The results of the DPPH assay showed strong antioxidative activities in the methanol- and water-extracts compared to the ethanol-extract, irrespective of the seed coat colors. Moreover, the soybean seeds with the black seed coat exhibited the highest antioxidant activity among the samples, regardless of the extraction solvent used. Eighteen bioactive compounds were subjected to data-mining processes including principal component analysis and hierarchical clustering analysis. Multivariate analyses showed that brown and black seeds were distinct from the yellow and green seeds in terms of the levels of carotenoids and anthocyanins, respectively. These results help our understanding of the compositional differences in the bioactive components among soybean seeds of various colors, providing valuable information for future breeding programs that seek to enhance the levels of compounds with health benefits.