• Journal of Nutrition and Health
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• 제15권1호
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• pp.9-14
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• 1982

The present paper describes a colorimetric method of determining the free and total unsaturated fatty acids in animal tissues. The procedure is based in three steps on the following principles : First step is that the tissue homogenates are extracted in chilled acetone in order to eliminate the interfering substance, such as phospholipids, sulfatides and proteins. At next step, after centrifugation acetone layer is decanted and evaporated to dryness. Then the extract is shaken with heptane to solve in the solvent. That the characteristic nature of copper salts of unsaturated fatty acids are freely soluble in heptane and those of saturated acids are not is the bases of separating one from another. Thus unsaturated fatty acids can be isolated in heptane as their salts from saturated acids and other lipid mixture by shaking with copper reagent. Finally the yellowish brown color developed by adding color reagent (0.2% sodium diethyldithiocarbamate in n-butanol solution) which reacts with the copper salts of the acids and produces the color is measured colorimetrically.

• 약학회지
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• 제46권4호
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• pp.237-241
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• 2002

Polyphenoloxidase activity (PPO) in the leaves of Erechitites hieracifolia was estimated by Warburg's manometric method. The emzyme was most reactive toward chlorogenic acid followed by caffeic acid. Diethyldithiocarbamate and potassium cyanide were shown powerful inhibition rate to the polyphenoloxidase from the leaves of Erechitites hieracifolia. We confirmed antioxidant activity of the leaves of Erechitites hieracifolia by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging method. Electrophorectic isoenzyme banding patterns of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were observed by native PAGE. The correlation of PPO and antioxidant enzymes is not investigated yet. That is need to further study.

• 대한화학회지
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• 제16권6호
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• pp.373-377
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• 1972

미량 구리의 sodium diethyl dithiocarbamate의 chelate를 ethyl acetate로 추출하고 이 추출액을 구리(원자번호 29번)의 이웃원소인 아연(원자번호 30번) 분말에 흡착시켰다. 추출액을 날려보낸 뒤에 $250kg/cm^2$ 으로 압축성형하여 구리의 $K_{\alpha}2(2{\theta}_{LiF} = 45.08^{\circ})$에서 형광 X-선을 측정한 결과 구리 $10{\mu}g$까지 측정할 수 있었으며 표준물질첨가법에 의하여 상대오차를 ${\pm}10$%이내로 낮출수가 있었다.

• 대한임상독성학회지
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• 제2권1호
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• pp.54-57
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• 2004

Disulfiram (tetraethylthiuram disulphid) is used in the treatment of chronic alcoholism since it causes an unpleasant aversive reaction to alcohol. It works by inactivating hepatic aldehyde dehydrogenase, leading to pronounced rise in the acetaldehyde concentration when ethanol is metabolized. Acetaldehyde causes alcohol sensitivity, which involve vasodilation associated with feeling of hotness and facial flushing, increased heart rate and respiration rates, lowered blood pressure, nausea, headache. One of its metabolites, diethyldithiocarbamate (DDC) can inhibit the enzyme dopamine $\beta$-hydroxylase (DBH), this may account for the profound refractory hypotension and hypothermia seen with the disulfiram-ethanol reaction (DER), resulting from norepinephrine depletion. This report is presents the case of a patient we met, who presented with hypothermia caused by the disulfiram-ethanol reaction, and along with a brief review of the subject.

• Toxicological Research
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• 제9권1호
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• pp.35-44
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• 1993

Role of rat cytochrome P-450 2E1(P-450 2E 1) in the metabolism of chlorzoxazone was examined by using several approaches' (1) selective inhibiton of catalytic activity in rat liver microsomes by diethyldithiocarbamate, (2) correlation of dimethylnitrosomine N-demethylation with chlorzoxazone 6-hydroxylation, and (3) immunoinhibition of catalytic activity with rabbit anti-rat P-450. The results indicated that P-450 2E1 is responsible for the metabolism of chlorzoxazone.

• 한국산업보건학회지
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• 제4권2호
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• pp.189-197
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• 1994

Analytic methods for Cr(VI) level in industrial hygienic field were suggested by the National Institute for Occupational Safety and Health(NIOSH method 7600, 7604). There were growing needs for measurement of Cr(III) and Cr(VI) levels simultaneously. Two analytical methods were suggested to determine Cr(III) and Cr(VI) levels simultaneously. The one is method by using reversed phase high peformance liquid chromatography(HPLC) and the other is by using ion exchange HPLC. The purpose of this work was to evaluate the usefulness of these two analytic methods. For the difference of ionic charges of Cr(III)-ethylendiamine tetraacetic acid(EDTA) chelate and $CrO_4{^-2}$, we could detect them simultaneously by ion exchange HPLC. Also, we attempted to determine the levels of Cr(III) and Cr(VI) chelated with sodium diethyldithiocarbamate(NaDDTC) by using reversed phase HPLC. The confirmation of Cr(III) and Cr(VI) were checked by fraction collector and nameless atomic absorption spectrometer. The optimal conditions for the formation of Cr(III)-EDTA chelate were two hours incubation period with pH 5. Cr(III)-EDTA and Cr(VI) in EDTA solution were successfully separated by anion exchange column using $Na_2CO_3/NaOH$ mixture as mobile phase. Peaks of Cr(III)-EDTA and Cr(VI) in EDTA were identified at 5 minutes and 7 minutes of retention time respectively by the ion exchange HPLC. The formation of Cr(III)-NaDDTC and Cr(VI)-NaDDTC chelates were twelve hours incubation period. Cr(III)-NaDDTC and Cr(VI)-NaDDTC chelates were separated by reversed phase column using methanol and water mixture as mobile phase. Peaks of Cr(VI)NaDDTC and Cr(III)-NaDDTC chelates were identified at 13 minutes and 26 minutes of retention time respectively by the reversed phase HPLC. Due to reduction of Cr(VI) to Cr(III), it seems to be not suitable for simultaneous determination of Cr(III)-NaDDTC and Cr(VI)-NaDDTC chelates by reversed phase HPLS. Simultaneos determination of Cr(III) and Cr(VI) by ion exchange HPLC was more accurate and simple method.

• 대한화학회지
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• 제35권4호
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• pp.389-396
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• 1991

침전 부선기술에 의해 물시료 중 극미량 비소의 분리 농축 및 정량에 관하여 연구하였다. 부유물질을 걸러낸 물시료 1.0l를 삼각 플라스크에 취하고 비소를 pH 8.5${\pm}$0.1에서 La(OH)$_3$ 침전과 함께 공침시켰다. 몰비 1:8의 sodium oleate와 sodium dodecyl sulfate 혼합 계면활성제를 가하여 침전을 소수성으로 만든 다음, 부선용기에서 질소기체를 bubbling하여 용액 표면으로 띄웠다. 뜬 침전을 감압에 의하여 유리거르게에 정량적으로 걸러 모으고 작은 부피의 1.0M 황산으로 녹여서 탈이온수로 정확하게 25.00 ml의 표선까지 묽혔다. 농축된 비소를 아르신으로 발생시켜 silver diethyldithiocarbamate의 착물을 만든 다음 분광광도법으로 정량하였다. 검정곡선은 원래의 용액 중에서 20 ng/ml까지 직선성이 좋았다. 분석 결과는 캠퍼스 폐수와 강물 중에서 비소의 함량이 8.2 ng/ml 와 1.0 ng/ml임을 보여 주었고, 이들 시료에 일정량의 비소를 첨가하고 분석한 결과로부터의 회수율은 각각 93${\%}$와 90${\%}$이었다. 이로서 본 방법이 여러 가지 물시료 중의 극미량 비소 정량에 응용이 가능함을 결론지을 수 있었다.

• Archives of Pharmacal Research
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• 제17권6호
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• pp.428-433
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• 1994

The methabolism and phamacokinetics of a mixed disulfide S-(N, N-diethyldithiocarbamoyl)-N-acetyl-L-cysteine (AC-DDTC) were studied in rats. Two metabolites of AC-DDTC following iv and po administration were indentified in plasma and liver by HPLC and GC, namely N, N-diethyldithiocarbamate (DDTC) and the methyl ester of DDTC (Me-DDTC). AC-DDTC was very unstable in vivo and could not be detected neither in plasma nor in urine. Pharmacokinetic parameters of DDTC following intravenous administration of AC-DDTC (20 mg/kg) were calculated. DDTC has a low affinity to rat tissue and the body clearance was $9.0{\pm}3.4mkl/mim/kg$. The mean residence time (MRT) was $11.5{\pm}16.3 min$. After oral administration of 20 mg/kg AC-DDTC, maximal plasma concenttion ($C_{max}$) was $3.8{\pm}0.2 nmol/ml$ and the bioavailability was 7.04%. $C_{max}$ for DDTC at a dose of 120 mg/kg. AC-DDTC was $40.1{\pm}2.2 nmol/ml$. ART was $47.1{\pm}2.8min$.at a dose of 20 mg/kg and $110.5{\pm}6.0 min$ at 120 mg/kg.

• 분석과학
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• 제20권1호
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• pp.41-48
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• 2007

유리소재 중의 미량의 납 및 카드뮴을 Na-DDTC를 가하여 Pb- 및 Cd-DDTC 착물을 형성시켜 유리 matrix 원소들로 부터 분리한 다음 MIBK로 추출하여 ICP-AES로 분석하였다. 유리 기질성분인 Na, Si, Mg 그리고 Ca 화합물 등은 미량의 납 및 카드뮴 정량에 방해작용을 하므로 직접 분석할 수 없으므로 용매추출법에 의해 Pb 및 Cd를 효율적으로 분리할 수 있었다. Pb 및 Cd 화합물이 첨가된 시험기준 시료와 NIST SRM 1412에 대한 용매추출실험에서 matrix의 영향을 받지 않고 정량하였다.

• 대한화학회지
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• 제44권1호
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• pp.17-23
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• 2000

전기화학적으로 중합한 $[Ru(v-bpy)_3]^{2+}$의 다가 양이은성 고분자 피막전극을 $PF6^-/ClO_4^-$대이온의 비가 1:1 정도 되게 한 후에 우라늄과의 착물의 안정도상수가 각각 38.6과 17.5인 xylenoI orange와 diethyldithiocarbamate로 변성한 전극을 제작하였다. 이를 이용하여 용액중의 U(VI)을 정량할 수 있는 여러 회 사용이 가능한 전극을 제작하였다. 이 때에 분석용 신호를 얻기 위한 전극은 Pt/p-$[Ru(v-bpy)_3]^{2+}$, ligand, U(VI)이며 염화은 기준전극을 사용하였다. 벗김전압전류 과정에서는 전자전달이 지배적인 과정이었으며, 검정선은 $1.0{\times}10^{-3}{\sim}1.0{\times}10^{-7}$ M 범위에서 0.99의 좋은 상관관계와 5${\sim}$8%의 상대 표준편차를 나타냈다.