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Properties of Polyphenoloxidase and Antioxidant Enzymes in the Leaves of Erechitites hieracifolia  

김안근 (숙명여자대학교 약학대학)
이상은 (STC 생명과학연구원 신약개발연구소)
김국환 (동덕여자대학교 약학대학)
권영이 (STC 생명과학연구원 신약개발연구소)
Publication Information
YAKHAK HOEJI / v.46, no.4, 2002 , pp. 237-241 More about this Journal
Abstract
Polyphenoloxidase activity (PPO) in the leaves of Erechitites hieracifolia was estimated by Warburg's manometric method. The emzyme was most reactive toward chlorogenic acid followed by caffeic acid. Diethyldithiocarbamate and potassium cyanide were shown powerful inhibition rate to the polyphenoloxidase from the leaves of Erechitites hieracifolia. We confirmed antioxidant activity of the leaves of Erechitites hieracifolia by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging method. Electrophorectic isoenzyme banding patterns of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were observed by native PAGE. The correlation of PPO and antioxidant enzymes is not investigated yet. That is need to further study.
Keywords
Polyphenol oxidase; DPPH; Superoxide dismutase; Peroxidase; Catalase; Erechitites hieracifolia;
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