• Journal of Nutrition and Health
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• v.15 no.1
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• pp.9-14
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• 1982

The present paper describes a colorimetric method of determining the free and total unsaturated fatty acids in animal tissues. The procedure is based in three steps on the following principles : First step is that the tissue homogenates are extracted in chilled acetone in order to eliminate the interfering substance, such as phospholipids, sulfatides and proteins. At next step, after centrifugation acetone layer is decanted and evaporated to dryness. Then the extract is shaken with heptane to solve in the solvent. That the characteristic nature of copper salts of unsaturated fatty acids are freely soluble in heptane and those of saturated acids are not is the bases of separating one from another. Thus unsaturated fatty acids can be isolated in heptane as their salts from saturated acids and other lipid mixture by shaking with copper reagent. Finally the yellowish brown color developed by adding color reagent (0.2% sodium diethyldithiocarbamate in n-butanol solution) which reacts with the copper salts of the acids and produces the color is measured colorimetrically.

• YAKHAK HOEJI
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• v.46 no.4
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• pp.237-241
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• 2002

Polyphenoloxidase activity (PPO) in the leaves of Erechitites hieracifolia was estimated by Warburg's manometric method. The emzyme was most reactive toward chlorogenic acid followed by caffeic acid. Diethyldithiocarbamate and potassium cyanide were shown powerful inhibition rate to the polyphenoloxidase from the leaves of Erechitites hieracifolia. We confirmed antioxidant activity of the leaves of Erechitites hieracifolia by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging method. Electrophorectic isoenzyme banding patterns of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were observed by native PAGE. The correlation of PPO and antioxidant enzymes is not investigated yet. That is need to further study.

• Journal of the Korean Chemical Society
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• v.16 no.6
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• pp.373-377
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• 1972

The analytical conditions for the fluorescence X-ray determination of small amount of cupper in solution were established. Copper in solution was extracted by diethyldithiocarbamate ethyl acetate and adsorbed by zinc powder. After drying, it was pressed to tablet under the pressure of $250Kg/cm^2.$$K_{\alpha}2(2{\theta}_{LiF} = 45.08^{\circ})$. The limit of detection was estimated to be about $10{\mu}g$ by the method of standard addition with an error of 10 %.

• Journal of The Korean Society of Clinical Toxicology
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• v.2 no.1
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• pp.54-57
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• 2004

Disulfiram (tetraethylthiuram disulphid) is used in the treatment of chronic alcoholism since it causes an unpleasant aversive reaction to alcohol. It works by inactivating hepatic aldehyde dehydrogenase, leading to pronounced rise in the acetaldehyde concentration when ethanol is metabolized. Acetaldehyde causes alcohol sensitivity, which involve vasodilation associated with feeling of hotness and facial flushing, increased heart rate and respiration rates, lowered blood pressure, nausea, headache. One of its metabolites, diethyldithiocarbamate (DDC) can inhibit the enzyme dopamine $\beta$-hydroxylase (DBH), this may account for the profound refractory hypotension and hypothermia seen with the disulfiram-ethanol reaction (DER), resulting from norepinephrine depletion. This report is presents the case of a patient we met, who presented with hypothermia caused by the disulfiram-ethanol reaction, and along with a brief review of the subject.

• Toxicological Research
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• v.9 no.1
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• pp.35-44
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• 1993

Role of rat cytochrome P-450 2E1(P-450 2E 1) in the metabolism of chlorzoxazone was examined by using several approaches' (1) selective inhibiton of catalytic activity in rat liver microsomes by diethyldithiocarbamate, (2) correlation of dimethylnitrosomine N-demethylation with chlorzoxazone 6-hydroxylation, and (3) immunoinhibition of catalytic activity with rabbit anti-rat P-450. The results indicated that P-450 2E1 is responsible for the metabolism of chlorzoxazone.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.4 no.2
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• pp.189-197
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• 1994

Analytic methods for Cr(VI) level in industrial hygienic field were suggested by the National Institute for Occupational Safety and Health(NIOSH method 7600, 7604). There were growing needs for measurement of Cr(III) and Cr(VI) levels simultaneously. Two analytical methods were suggested to determine Cr(III) and Cr(VI) levels simultaneously. The one is method by using reversed phase high peformance liquid chromatography(HPLC) and the other is by using ion exchange HPLC. The purpose of this work was to evaluate the usefulness of these two analytic methods. For the difference of ionic charges of Cr(III)-ethylendiamine tetraacetic acid(EDTA) chelate and $CrO_4{^-2}$, we could detect them simultaneously by ion exchange HPLC. Also, we attempted to determine the levels of Cr(III) and Cr(VI) chelated with sodium diethyldithiocarbamate(NaDDTC) by using reversed phase HPLC. The confirmation of Cr(III) and Cr(VI) were checked by fraction collector and nameless atomic absorption spectrometer. The optimal conditions for the formation of Cr(III)-EDTA chelate were two hours incubation period with pH 5. Cr(III)-EDTA and Cr(VI) in EDTA solution were successfully separated by anion exchange column using $Na_2CO_3/NaOH$ mixture as mobile phase. Peaks of Cr(III)-EDTA and Cr(VI) in EDTA were identified at 5 minutes and 7 minutes of retention time respectively by the ion exchange HPLC. The formation of Cr(III)-NaDDTC and Cr(VI)-NaDDTC chelates were twelve hours incubation period. Cr(III)-NaDDTC and Cr(VI)-NaDDTC chelates were separated by reversed phase column using methanol and water mixture as mobile phase. Peaks of Cr(VI)NaDDTC and Cr(III)-NaDDTC chelates were identified at 13 minutes and 26 minutes of retention time respectively by the reversed phase HPLC. Due to reduction of Cr(VI) to Cr(III), it seems to be not suitable for simultaneous determination of Cr(III)-NaDDTC and Cr(VI)-NaDDTC chelates by reversed phase HPLS. Simultaneos determination of Cr(III) and Cr(VI) by ion exchange HPLC was more accurate and simple method.

• Journal of the Korean Chemical Society
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• v.35 no.4
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• pp.389-396
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• 1991

The pre-concentration and determination of ultratrace arsenic in water samples was studied by the precipitate flotation technique. The arsenic in 1.0l of water sample, in which all suspended materials were filtered out, was coprecipitated together with La(OH)$_3$ precipitates at pH 8.5${\pm}$0.1. After the precipitate was made to be hydrophobic by adding mixed surfactant of 1 : 8 mole ratio of sodium oleate and sodium dodecyl sulfate, it was floated with the aid of tiny bubbles of nitrogen gas in a flotation cell. The floated precipitate was quantitatively collected on a micropore glass filter by the suction, dissolved with small volume of 1.0M sulfuric acid, and accurately diluted to 25.00ml with a de-ionized water. Total arsenic was spectrophotometrically determinated by forming silver diethyldithiocarbamate complex of arsine generated from arsenic in the concentrated solution. The calibration curve was linear up to 20ng/ml in the original solution. Analytical results showed that contents of arsenic in a campus wastewater and a river water were 8.2ng/ml and l.0ng/ml, respectively, and their recoveries were 93${\%}$ and 90${\%}$ in water samples which a given amount of arsenic was added into. From above result, it could be concluded that this method was applicable to the determination of arsenic in various kinds of water at low ng/ml levels.

• Archives of Pharmacal Research
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• v.17 no.6
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• pp.428-433
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• 1994

The methabolism and phamacokinetics of a mixed disulfide S-(N, N-diethyldithiocarbamoyl)-N-acetyl-L-cysteine (AC-DDTC) were studied in rats. Two metabolites of AC-DDTC following iv and po administration were indentified in plasma and liver by HPLC and GC, namely N, N-diethyldithiocarbamate (DDTC) and the methyl ester of DDTC (Me-DDTC). AC-DDTC was very unstable in vivo and could not be detected neither in plasma nor in urine. Pharmacokinetic parameters of DDTC following intravenous administration of AC-DDTC (20 mg/kg) were calculated. DDTC has a low affinity to rat tissue and the body clearance was $9.0{\pm}3.4mkl/mim/kg$. The mean residence time (MRT) was $11.5{\pm}16.3 min$. After oral administration of 20 mg/kg AC-DDTC, maximal plasma concenttion ($C_{max}$) was $3.8{\pm}0.2 nmol/ml$ and the bioavailability was 7.04%. $C_{max}$ for DDTC at a dose of 120 mg/kg. AC-DDTC was $40.1{\pm}2.2 nmol/ml$. ART was $47.1{\pm}2.8min$.at a dose of 20 mg/kg and $110.5{\pm}6.0 min$ at 120 mg/kg.

• Analytical Science and Technology
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• v.20 no.1
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• pp.41-48
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• 2007

The determination method for the trace lead and cadmium in glass materials was developed by ICP-AES. The lead- and cadmium-DDTC chelates were formed by adding Na-DDTC (sodium diethyldithiocarbamate) as a chelating agent and extracted into MIBK (methyl-isobutyl ketone). Direct measuring of the trace lead and cadmium in glass was not possible because of the interferences of matrix elements as Na, Si, Mg and Ca of glasses unless solvent extraction of lead and cadmium was applied. This procedure was confirmed to be valid quantitative method of Pb and Cd analysis in glass did not influence of sample matrix, by applying it procedure to artifact sample and NIST SRM 1412.

• Journal of the Korean Chemical Society
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• v.44 no.1
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• pp.17-23
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• 2000

Electrodes of the polycationic film with electropolymerized $[Ru(v-bpy)_3]^{2+}$ having about 1:1 ratio of $PF6^-/ClO_4^-$as the doped counter ions, were modified with xylenol orange and diethylditbiocarbamate by ion exchange which had stability constant as 38.6 and 17.5 respectively. These electrodes were employed in the quantitative multiple determination of U(W) in solution. The working electrode of electrochemical cell for the analytical signal was Pt/p-$[Ru(v-bpy)_3]^{2+}$, ligand, U(VI) with Ag/AgCl reference elecrode. In the stripping voltammetry. electrode process was electron transfer controlled one and calibration curves at the ranges of $1.0{\times}10^{-3}{\sim}1.0{\times}10^{-7}$ M had excellent relationship as 0.99 and relative standard deviation as 5${\sim}$8%.