• Journal of Nutrition and Health
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• v.30 no.3
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• pp.266-276
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• 1997

To investigate the effects of dietary protein and calcium levels on calcium and bone metabolism Sprague-Dawley male growing rats weighting approximately 91.4g were divided into four groups and fed one of the following four experimental diets-15% protein 0.2% calcium ; 15% protein 0.5% calcium ; 30% protein 0.2% calcium ; 30% protein 0.5% calcium-for five weeks. Calcium intake and excretion, apparent calcium absorption were measured and bone densities and mineral contents of femur and scapula were analyzed. Calcium excretion through feces and urine was significantly greater in animals receiving diets of higher calcium. Fecal calcium but not urinary calcium excretion was greater when the protein level was increased from 15% to 30%. Apparent calcium absorption rate was significantly higher with lower calcium intakes. Serum alkaline phosphatase activity was significantly higher in 0.2% calcium group than in 0.5% calcium group, while urinary hydroxyproline excretion was essentially same among all experimental groups. Weights and mineral contents or protein. Bone weights were greater, but calcium and ash contents of femur and scapula were lower in animals on the diet containing low calcium and high protein, which suggests that bone metabolism may be affected by the interaction between calcium and protein intake. These results indicate that during growth high protein intake might be beneficial to bone health if the diet is sufficient in calcium, however, if the diet fails to provide an optimum amount of calcium, such practice might be detrimental.

• Journal of the Korean Society of Food Culture
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• v.15 no.5
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• pp.368-378
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• 2000

This study was conducted to assess dietary intake and quality obtained by 24-hour recall method between Korean-Chinese in Yanbian, China(Korean-Chinese) and Koreans in Kuri, Korea(Koreans). The subjects consisted of 730 Korean-Chinese and 696 Koreans. Mean daily energy intake of Korean-Chinese was 1,788kcal and that of Korean was 1,844kcal. Proportions of energy intake from carbohydrate, fat, and protein were 62.6: 16.9: 14.2 for Korean-Chinese and 65.4: 16.3: 14.4 for Koreans. Nutrient intake levels of Koreans were higher than those of Korean-chinese in most nutrients. Calcium and vitamin $B_2$ intakes as percent of Korean RDA were less than 60% in Korean-Chinese. Koreans consumed calcium and vitamin A in the level less than 80% of Korean RDA. Iron intake level of Korean females was less than 75% of Korean RDA. Korean-Chinese consumed 90.4% of food from plant origin, and Koreans consumed 82.5% of food from plant origin. Mean dietary diversity score(DDS) of Korean-Chinese, $3.00{\pm}0.39$, was significantly lower than that of Koreans, $3.73{\pm}0.76(p<0.05)$. Mean dietary variety score(DVS) of Korean-Chinese, 13.6, was significantly lower than that of Koreans, 20.1(p<0.001). According to these results, some adults in the both Korean-Chinese and Koreans did not consume enough nutrients, especially calcium and vitamin $B_2$ intake for Korean-Chinese and calcium and vitamin A intake for Koreans. Dietary qualities of Korean-Chinese subjects and Korean females were not adequate. In the future, further research to promote dietary quality of Koreans including Korean-Chinese must consider the results of this study.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.2
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• pp.197-202
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• 1999

Three barrows weighing 45.0 kg, fitted with simple T-cannulas in both the duodenum and terminal ileum, were assigned to diets in a $3{\times}3$ Latin Square design experiment to determine the effect of two calcium levels (0.8% vs 0.4%) on phytase activity and nutrient balance in growing pigs. The control diet contained 0.8% calcium, with no added inorganic phosphorus (0.45% total phosphorus) and no added phytase. The two additional experimental diets contained microbial phytase (750 phytase units/kg) and supplied either 0.8% or 0.4% calcium. With added microbial phytase, ileal and total tract digestibility of rotal phosphorus were improved by 20.9 and 13.8 percentage units, respectively (p=0.01). The apparent duodenal and ileal digestibility of phytate phosphorus were increased by 51.8 and 49.7 percentage units (p=0.01). Lowering dietary calcium in the presence of microbial phytase increased the digestibility of phytate phosphorus by an additional 10.9 (p=0.001) and 5.7 percentage units for duodenal and ileal digestibility, respectively. Supplementation with microbial phytase significantly reduced fecal excretion of nitrogen and phosphorus and increased the percentage of these nutrients retained by the pig. Lowering dietary calcium further increased the percentage of dietary phosphorus retained. Overall, reducing dietary calcium appeared to increase the effectiveness of added microbial phytase in degrading phytate phosphorus. As a result, care should be taken to avoid high levels of dietary calcium when supplementing swine diets with microbial phytase.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.2
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• pp.296-304
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• 1998

The effects of common Korean dietary fiber-rich foods(dried sea mustard, Lentinus edodes and Korean radish leaves) on total gut transit time of diet, serum cholesterol level and mineral apparent absorption ration and tissue level were investigated. Twenty eight male were divided into 4 groups and were fed a control diet(containing 2% cellulose) and three fiber supplementary diet mixed with 10% of the above dietary fiber rich foods for 4 weeks, respectively. Body weight gain and food efficiency ration were not affected by the supplementary diet of fiber but the total gut transit time of diet was shortened in all dietary fiber groups. The Lentinus edodes reduced significantly serum total cholesterol and increased the HDL-cholesterol/total cholesterol ratio at 4th week without and effect on calcium and iron apparent absorptions and tissue levels of the minerals. Radish leaves decreased significantly iron apparent absorption ration with a tendency to increase iron retention in spleen and did not alter serum cholesterol level. Serum cholesterol concentration and calcium and iron apparent absorptions were not significantly altered by the ingestion of sea mustard, while it had more soluble dietary fiber than mushroom and radish leaves.

• Korean Journal of Rural Living Science
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• v.7 no.1
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• pp.39-50
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• 1996

This study was carried out to investigate nutrients intake and dietary environments of rural and asylum elderly in partial Kyunggi area. Results were as followes: (1) Educational level of 2 groups of rural and asylum elderly was only literacy, And rural elderly worked more than the aged of asylum. (2) The nutrients intake of rural elderly was very small quantity: Energy intake was 62% of RDA, that of protein 59%, calcium 56%, and iron 72% only. This nutrients intake state was lower than that of asylum elderly. (3) Health score CMI was selected 31 cases of items for aged. Ryal elderly received 11.7/31, but the scores of asylum elderly were 7.1 only. It means that asylum elderly felt more healthy than rural. (4) The correlation Serum TG was significant with protein fat and calcium, but serum calcium was not significant with intake of calcium. The nutrients intake was correlated with CMI score and dietary habit score.

• Journal of Community Nutrition
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• v.4 no.3
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• pp.159-163
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• 2002

A survey was performed to 238 male young men who were living alone (104 persons) or living with roommates (134 persons). Their average age was 22.6 years old, height 171.8cm, and weight 65.6kg. Their daily calcium intake level was averaged 271.9 $\pm$ 169.1mg, which was 38.8% of Korean Recommended Dietary Allowances(RDA). Comparing calcium intake of each mealtime, breakfast, lunch, dinner, and snacks were composed of 18.6%, 30.0%, 34.0% and 17.4%, respectively. Their daily iron intake level was averaged 8.9 $\pm$ 10.8mg, which was 74.3% of Korean RDA. Comparing iron intake of each mealtime, breakfast, lunch, dinner, and snacks were composed of 15.4%, 33.6%, 36.3% and 14.7%, respectively. Deficiency of these minerals in young male adults were severe and the nutrient qualities were also poor and the deficiency was more profound in calcium than in iron. And we observed iron nutrition differed significantly according to their living status, such as iron intake and its %RDA were significantly worse in single living subjects than the other. However calcium did not show significant difference. It was found mineral intake, especially iron, could be improved in young men living with roommates than living alone and regular breakfast could improve calcium and iron intakes. (J Community Nutrition 4(3) : 159~5163, 2002)

• Journal of Nutrition and Health
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• v.28 no.9
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• pp.817-824
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• 1995

This study was performed to evaluate the effect of dietary protein level on Ca efficiency in bone mineral density in growing male rats. Twenty male rate were divided into two groups. The rats in one group were fed on casein 20% diet as control group and the others were fed on casein 40% diet as protein group. All rats were fed on experimental diet and deionized water ad libitum for 9 weeks. The total body, spine and femur bone mineral density and bone mineral content were measured using dual energy-x ray absorptiometry. Urinary calcium, phosphate, pyridinoline and creatinine, serum calcium, phosphate, total protein, albumin, alkaline phosphatase(ALP) and osteocalcin were measured. Urinary Ca excretion, pyridinoline and crosslinks value and serum ALP content seem to be increased in high protein group. It appears that the growing rats in high protein group had a higher bone resprption and bone formation than those in control group. Animal fad a high protein diet had a siginficantly higher Ca efficiency in BMD, BMC of total body, spine and femur. The results of this show that increasing of dietary protein level (40%) is beneficial of improvement of Ca efficiency during growing period.

• Preventive Nutrition and Food Science
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• v.2 no.1
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• pp.55-60
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• 1997

A lysosomal and matrix degrading enzyme $\beta$-glucuronidase activity was measured in BALS/c mice fed high and low Ca in combination with the i.p. adminstration of calcium-regulating hormones including parathyoid hormone(PTH), calcitonin(CT) and cholecalciferol(Vit D). After feeding experimental diets for five weeks, mice were sacrificed by cervical dislocation and the enzyme was fluometrically measured at 440nm. $\beta$-Glucuronidase activity was inhibited by high calcium in the diet. in addition, vitamin D also inhibited the enzyme activity in the serum regardless of the level of dietary calcium. In contrast, PTH has shown to stimulate the enzyme at all the levels of dietary calcium. Calcitonin, and inhibitor of PTH action for bone resorption, revealed to curb PTH effect in this enzyme, whereas CT stimulated the action of vitamin D in the serum. The above results led us to conclude that osteoclastic bone resorption and senile osteoporosis may be reduced by adequate dietary calcium and vitamin D.

• Korean Journal of Rural Living Science
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• v.6 no.1
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• pp.31-39
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• 1995

This study was performed to observe how soy protein and calcium in rats fed unsaturated fat-enriched diet affect the total lipid and cholesterol contents of blood and tissues male Sprague-Dawley rats weighing approximately 220g were fed four purified diets which contained 18% (w/w) corn oil, 1% (w/w) cholesterol, two sources of protein : casein or isolated soy protein (ISP) and two levels of dietary calcium : 0.1% or 1.0%, first for four weeks (Expt. 1) and second for eight weeks (Expt. 2). The contents of total lipid, cholesterol and triglyceride in blood, liver, heart and feces were determined. After four weeks feeding, the serum lipid and cholesterol concentration was not significantly different among the groups. After eight weeks feeding, these concentrations were significantly lower in ISP-High calcium group. The serum lipid concentration was influenced by dietary protein sources and calcium levels. These results indicated that the hypolipidemic effects of soy protein and high calcium intake were partly due to decrease in lipid absorption and these effects were not detected at 4 weeks feeding but 8 weeks feeding.

• Journal of the Korean Home Economics Association
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• v.29 no.3
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• pp.61-69
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• 1991

The relationships between nutrients intake, physical activity and bone mineral density were investigated in 19 elderly men aged 71-80 years. A trained nutritionist interviewed usual dietary intake and daily activity with a questionnaire, and bone mineral density was measured at the lumbar spine and three regions of the proximal femur (femur neck, Ward's triangle and trochanter) with a Luna DP3 dual photon absorptiometry. The correlations between dietary calcium intake and bone mineral density at the lumbar spine and trochanteric region were significant at P<0.05 and P<0.01 level respectively. the significant correlations were also found between vitamin A(P<0.005), riboflavin(P<0.01), and ascorbic acid(P<0.05) intake and bone mineral density at these sites. Higher physical activity was associated with greater bone mineral density of four sites, but this was not significant. But there were significant relationships between total energy expenditure and bone mineral density of the lumbar spine(P<0.01), femur neck (P<0.05) and Ward's triangle(P<0.05). In this study the results revealed that bone mineral density of the lumbar spine and trochanteric region were associated with dietary calcium intake. And bone mineral density of the femur neck and Ward's triangle were related to physical activity but not to nutrients intake. In conclusion, dietary calcium intake seems to be a important factor for greater bone mineral density. Further evidence will be needed that physical activity protects against bone fracture and osteoporosis in the edlerly.