• 대한지역사회영양학회지
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• 제8권3호
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• pp.356-367
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• 2003

This study was to investigate the effects of a silk peptide, and prune and raspberry mixture on the body fat, serum lipid composition and fat distribution in Korean women during the time they received these supplements. During the 4 month period of this research (June to October, 2002), 75 women (average age 22.9 yr) were selected as subjects. Their nutrient intake was investigated by use of a questionnaire and the 24-hour recall method. Antropometric assessments of the subjects were investigated by using the SBIA Method (Segmental Bioimpedance Assay, Inbody 3.0). The results were as follows: their mean body weight was 59.3 kg, their mean body height was 161.7 cm and their mean Basal Metabolic Indices (BMI) was 22.7. The status of their dietary fiber intake significantly increased (p < 0.05) after use of the supplements. Their body fat significantly decreased following the $3^{rd}$ stage of supplementation (p < 0.05), and their percent of body fat signiflcantly decreased after supplementation (p < 0.05). Their triceps and suprailiac circumference significantly decreased following the $3^{rd}$ stage of supplementation (p < 0.05). Their waist circumferences and waist-hip ratios (WHR) significantly decreased after both the $2^{nd}$and $3^{rd}$ stage of supplementation (p < 0.05). Their total cholesterol significantly decreased following the $3^{rd}$ stage of supplementation, as compared to their baseline results and those obtained following the 1$^{st}$ stage of supplementation (p < 0.05). Their LDL-cholesterol and LDL / HDL ratios significantly decreased following the $3^{rd}$ stage of supplementation, as compared to their baseline results and those obtained following the 1$^{st}$ stage of supplementation (p < 0.05). Their 4-HNE and total antioxidant statuses significantly decreased following the $2^{nd}$ and $3^{rd}$stage of supplementation, as compared to their baseline results and those obtained following the $1^{st}$ stage of supplementation (p <0.05). Their $2^{nd}$ stage supplementation results were significantly negatively correlated with their triceps measurements and WHR (p < 0.05). Their $3^{rd}$stage supplementation results were significantly negatively correlated with their anterior thigh measurements (p < 0.05). The above results show that prune, raspberry, silk peptide and dietary fiber supplementation to a routine diet improves body fat distribution, total cholesterol, and triglyceride levels. The decrease of abdominal fat and WHR were especially noteworthy. This implies a decrease of risk factors. (Korean J Community Nutrition 8(3) : 356~367, 2003)003)

• Asian-Australasian Journal of Animal Sciences
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• 제19권8호
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• pp.1179-1189
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• 2006

This experiment was designed to examine the effects of supplemental vitamin D on laying performance, metabolic profile and egg quality of hens fed diets containing different fat sources and levels. Lohman strains (n = 480) were assigned to one of 10 diets: basal diet (BD), BD plus 2.5 and 5.0% sunflower oil (SO) or tallow (T) at vitamin D provided $1{\times}$ and $3{\times}$ of the current recommendation. The experiment lasted from week 30 to 44 of age. Each diet was tested in 12 replicate cages of 4 hens. Production, metabolism, and egg quality data were subjected to three-way ANOVA. Both fats decreased feed intake (FI) as compared to BD. Increasing SO and T levels linearly decreased and quadratically increased FI, respectively. The dietary factors did not affect egg production (EP) and egg weight. Vitamin D supplementation increased and decreased EP when diets contained SO and T, respectively. Feed conversion efficiency (FCE) for hens fed SO was lower than for hens fed T. However, increasing T level improved FCE, whereas increasing SO level worsened FCR. Vitamin D supplementation increased serum vitamin D and glucose concentrations. Vitamin D supplementation also caused a decrease and an increase in serum vitamin D concentration when diets contained SO and T, respectively. Serum glucose concentration for hens fed SO was lower than hens fed T. Increasing fat level linearly increased serum triglyceride and VLDL concentrations, regardless of the fat type. Increasing SO level linearly decreased serum cholesterol concentration. Vitamin D supplementation did not alter lipid metabolites. The dietary factors did not affect serum total protein, Ca, and P concentrations. As compared with BD, feeding SO decreased dry tibia and ash weights more than feeding T. Vitamin D supplementation tended to increase dry tibia weight and decrease tibia ash weight. Eggshell strength and thickness, yolk and albumen indexes, and Haugh unit were not responsive to the dietary factors. Eggshell strength quadratically increased with increasing T level. Yolk color for hens fed SO was lower than for hens fed T. The dietary factors did not affect most of yolk fatty acids. Increasing SO level quadratically decreased yolk $C_{18:2}$ concentration. Vitamin D supplementation increased and decreased yolk $C_{18:2}$ concentration when diets contained SO and T, respectively. In conclusion, increasing fat level improved laying performance without altering metabolic profile and egg quality. Vitamin D supplementation had minor alteration effects on laying performance, metabolic profile, and egg quality in response to fat feeding.

• Journal of Nutrition and Health
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• 제42권5호
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• pp.415-422
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• 2009

본 연구에서는 포도박이 고지방식이를 섭취한 흰쥐의 효소 활성과 지질과산화 수준에 미치는 영향을 조사함으로써 포도박의 생리활성과 자원화에 필요한 기초자료를 얻고자하였다. 고지방식이를 섭취한 흰쥐에게 포도박 실험식이를 급여한 후 혈청, 간조직 중의 지질과산화물 함량, glutathione 함량과 간조직 효소 활성을 측정하였다. 포도박 첨가군의 식이섭취량은 대조군에 비하여 감소하였고, 체중증가량의 실험군 간 유의적인 차이는 없었다. 식이효율은 고지방식이군이 정상식이군 보다 유의적으로 증가하였다. 지질과산화물 함량은 혈청의 경우 정상식이군에서는 포도박 첨가에 의한 변화가 없었으나 고지방식이군에서 함량이 증가되었고 포도박 첨가에 의해 감소되었다. 간조직과 간 microsome에서는 포도박을 첨가한 군이 각각의 대조군에 비하여 유의적으로 감소하였다. 간조직 내의 총glutathione 함량과 GSH/GSSG 비는 포도박 첨가군이 대조군에 비하여 모두 유의적으로 증가하였다. 간조직의 SOD 활성은 정상식이군에서는 차이가 없었으나 고지방식이에 포도박을 첨가한 군이 고지방대조군에 비하여 활성이 유도되었다. 간조직의 catalase 활성은 대조군에 비하여 포도박 첨가군이 유의적으로 증가하였다. G6Pase 활성은 포도박의 첨가로 인하여 대조군보다 활성이 증가하였지만 실험군간 유의적인 차이는 없었다. GST와 GSH-Px 활성은 정상 식이군에서는 변화되지 않았으나 고지방식이에 포도박을 첨가한 군이 고지방대조군 보다 효소 활성이 유의적으로 증가되었다. 이상의 결과를 종합해 보면 식이섬유와 폴리페놀 성분을 다량 함유한 포도박 식이는 체내 항산화계를 활성화함으로써 지질 산화와 관련성이 높은 심혈관계 질환의 예방효과를 가져올 수 있는 것으로 기대되었다. 포도박의 이러한 생리활성에 대한 연구결과는 향후 포도 가공 중에 얻어지는 포도박 폐기물을 자원화 할 수 있는 기초자료로 이용될 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제3권1호
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• pp.33-38
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• 1990

Fifteen Landrace pigs were used to determine the effects of supplemental copper or kapok meal, or both on the characteristics of the depot fat. The pigs were allocated into five groups of 3 pigs each. The animals were fed diets as follows: (1) control diet 100%; (2) control diet plus 20 mg/kg copper; (3) control diet plus 200 mg/kg copper; (4) control diet plus 3% kapok meal; (5) control diet plus 200 mg/kg copper and 3% kapok meal. In addition, 100 mg/kg iron and 100 mg/kg zinc were supplemented to all the diets. The pigs were slaughtered at the same time after 8 weeks of feeding period at an average weight of 98.6 kg. The supplementation of 20 or 200 mg/kg dietary copper did not affect the fatty acid compositions, the iodine numbers, the melting points or the copper contents of the inner and outer layers of backfat and the perirenal fat from pigs. Supplementation of kapok meal significantly elevated the melting point, the content of C18:0 and the ratio of C18:0/C18:1 of the porcine fats and decreased the content of C16:1. There was no interaction noted between copper and kapok meal on the porcine fat characteristics. These results indicate that the swine diet supplemented up to 200 mg/kg copper with zinc and iron can be used in growing-finishing swine diets without affecting either porcine fat characteristics or hardening effect of kapok meal on porcine fat.

• Journal of Nutrition and Health
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• 제26권5호
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• pp.578-585
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• 1993

This study was performed to investigate effects of low fat diet and saturated fat supplementation on the function of the immune system. Forty male BALB/c mice average-weighing 15g were divided into two dietary groups: 0.7% safflower oil group and 4.3% beef tallow & 0.7% safflower oil group. Results are as follows; 1) Food intake, body weight, organ weight, agglutination test, differential white cell count and histological examination of spleen were not different in two dietary groups during the experimental period. 2) Delayed-type hypersensitive test of the mice fed 4.3% beef tallow & 0.7% safflower oil was significantly higher than that of the mice fed 0.7% safflower oil ($\alpha$=0.05). 3) Plaque forming cell was significantly reduced at 10th week compared to 7th week in both groups($\alpha$=0.05). Although there was no significant difference between two groups. 0.7% safflower oil groups showed slightly higher plaque forming cell than 4.3% beef tallow & 0.7% safflower oil group.

• Journal of Nutrition and Health
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• 제26권5호
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• pp.566-577
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• 1993

The study was to compare the effect of dietary fatty acids on fatty acid profile in tissue and the status of tocopherol and lipid peroxidation, and superoxide dismutase and glutathione peroxidase activities at two fat levels. Male Sprague Dawley rats weighing average 350g(17 weeks) were fed either low fat(LF, 4.3% w/w, 10% kcal) or high fat(HF, 20.8%, w/w, 40% kcal)diet for 6 weeks. The fats used were beef tallow as a source of saturated fatty acid, corn oil for n-6 linoleic acid, perilla oil for n-3 $\alpha$-linolenic acid and fish oil for n-3 eiocosapentatenoic acid(EPA) and n-3 docosahexaenoic acid(DHA). Palsma tocopherol was significantly reduced by fish oil compared to beef tallow at body fat level. However, there was no significant effect on the levels of plasma MDA, RBC MDA and tocopherol, and RBC hempolysis by the type and amount of dietary fat. The peroxidizibility index of fatty acid profile in plasma and liver was increased and liver MDA level was significantly increased by fish oil when dietary fat level was increased. The activities of SOD and GSHPx tended to be increased by perilla oil and fish oil at both fat oil significantly reduced the incorpration of c20:4 and increased the incorporation of c20:5 into liver compared to corn oil. The incorporation of n-3 fatty acids into tissue by perilla oil rich in $\alpha$-linolenic acid was significantly higher tan corn oil and its effect was improved with higher amount of perilla oil in diet by high fat diet. Overall, the lipid peroxidation of tissue could be prevented by tocopherol supplementation when dietary fat level was low in diet. However, at high fat diet, tocopherol supplementation might not be enough to prevent the lipid peroxidation in tissue since the potential for lipid peroxidation was tended to be increased with higher incorporation of higher unsaturated n-3 fatty acids into tissue. Therefore, it could not be recommended to consume large amount of fish oil even with excess amount of tocopherol supplemented to the high fat diet.

• Preventive Nutrition and Food Science
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• 제8권3호
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• pp.249-252
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• 2003

This study was conducted to determine the effects of chromium-methionine (CrMet) supplementation at various levels on obesity index, body fat, and serum glucose, insulin and leptin in rats. Forty male Sprague-Dawley rats were randomly assigned to one of four dietary groups and fed AIN-76 semi-purified basal diets supplemented with 0, 300, 600 or 1200 ppb Cr from CrMet. After 4 weeks on the respective diets, the rats were killed and serum glucose, insulin and leptin concentrations were determined. The CrMet supplementation did not affect weight gain, feed intake or feed efficiency ratio, fasting glucose, insulin or leptin levels among treatment groups. Although final body weight in all treatments were not significantly different, naso-anal length was longer in the 1200 ppb CrMet group than those of control or other groups (p<0.05). The lowest obesity index and body fat were observed in the 1200 ppb dietary group (p<0.05). The obesity index of the rats fed 1200 ppb supplemental CrMet was lower than in the other groups. These results suggest that CrMet supplementation results in a significant decrease in obesity index, possibly by decreasing the body fat that corresponded to increasing CrMet dosage.

• Journal of Nutrition and Health
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• 제31권7호
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• pp.1112-1120
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• 1998

This study was conducted to investigate the effets of chitosan and beef tallow at different level on glucose and lipid metabolism in rats. Dietary fot level was 20% and 40%, and chitosan was given at levels of 0%, 3%, and 5%(wt/wt) of diet. Chitosan supplement tended to decrease the serum total lipids, total cholesterol, and triglycerides. HDL cholesterol and HDL cholesterol : total cholesterol ratio tended to increase with 5% chitosan supplementation. LDL cholesterol and VLDL triglyceride tended to decrease with chitosan supplementation. Lipid concentration of liver and epididymal fat pad(EEP) tended to decrease with medium dietary fat and chitosan treatment. fecal excretion of total lipid and triglyceride exhibited a tendency to increase with high fat levels and chitosan. Length of small intestine and gastrointestinal transit time were not affected by dietary fit levels or chitosan supplements. Therefore, it could be suggested that chitosan supplement had beneficial effects on lipid metabolism. (Korean J Nutrition 31(7) : 1112-1120, 1998)

• Asian-Australasian Journal of Animal Sciences
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• 제24권5호
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• pp.650-654
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• 2011

An experiment involving 180 straight run one-day-Cobb broilers was conducted to evaluate the effect of supplementation of L-leucine with different levels of crude protein (CP) on carcass composition and sensory characteristics of broiler grower-finisher chickens. Six experimental diets comprising two levels of crude protein (CP) i.e., 20 and 20% with three levels of L-leucine i.e. 0, 0.5 and 0.67%, were offered to birds from 21-42 d of age. The birds were randomly divided into 36 experimental pens, 5 chickens in each pen, and there were 6 replicates under each diet. L-leucine supplementation did not affect the bone and lean, whereas fat was decreased (p<0.05) when L-leucine was added at 0.5%. Similarly, there were no significant differences (p>0.05) in the lean, fat and bone among chickens fed two levels of CP. No significant differences between dietary treatments were observed on any sensory characteristics affected by dietary L-leucine and CP. From this study, it is obvious that supplementation of up to 0.5% L-leucine reduced fat. However, other characteristics were not affected by supplementation of L-leucine. Similarly, reduction of body composition and sensory characteristics were not apparent on a diet low in CP.

• Asian-Australasian Journal of Animal Sciences
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• 제18권8호
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• pp.1105-1109
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• 2005

The effect of dietary fatty acid supplementation on milk production, milk composition and plasma constituents in dairy cows was examined. Dietary fatty acids including mainly palmitic acid and stearic acid were given to cows from 2 weeks before and 8 weeks after parturition. Weekly gain of daily milk production was increased gradually during early lactation period and reached a plateau at 4 weeks after parturition. Weekly gain of daily milk production in lactating cows received dietary fatty acids was significantly higher than that of cows given a control diet alone. Although milk lactose concentration was slightly decreased by dietary fatty acid supplementation, milk fat and protein were not significantly influenced by dietary fatty acid supplementation. Dietary fatty acids did not affect plasma concentrations of triglyceride, non-esterified fatty acids, total cholesterol, high-density lipoprotein cholesterol and glucose during postpartum. It is suggested that dietary fatty acid supplementation has the potency to enhance energy balance and improve milk yield without any adverse effects on milk composition.