• Preventive Nutrition and Food Science
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• v.2 no.1
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• pp.55-60
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• 1997

A lysosomal and matrix degrading enzyme $\beta$-glucuronidase activity was measured in BALS/c mice fed high and low Ca in combination with the i.p. adminstration of calcium-regulating hormones including parathyoid hormone(PTH), calcitonin(CT) and cholecalciferol(Vit D). After feeding experimental diets for five weeks, mice were sacrificed by cervical dislocation and the enzyme was fluometrically measured at 440nm. $\beta$-Glucuronidase activity was inhibited by high calcium in the diet. in addition, vitamin D also inhibited the enzyme activity in the serum regardless of the level of dietary calcium. In contrast, PTH has shown to stimulate the enzyme at all the levels of dietary calcium. Calcitonin, and inhibitor of PTH action for bone resorption, revealed to curb PTH effect in this enzyme, whereas CT stimulated the action of vitamin D in the serum. The above results led us to conclude that osteoclastic bone resorption and senile osteoporosis may be reduced by adequate dietary calcium and vitamin D.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.8
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• pp.1144-1148
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• 2001

The implications of incorporating deoiled rice bran (DORB) in diet on the utilization of certain nutrients had been examined through balance studies made with 6- and 10- week-old Japanese quails. The stuffs evaluated in this study had their origin from two different types of processes- a batch extraction (DORB-B) and another continuous process (DORB-C), and each included at 10, 20 or 30% level in an iso-nitrogenous and iso-caloric diet. On chemical analyses, the DORB-B was found to contain crude protein, 19.0; ether extract, 0.79; total ash, 17.05; Ca, 0.11; P, 1.92; glucose, 2.35 and starch 11.22 g/100 g day matter. Corresponding values for DORB-C were 15.02, 1.56, 13.0, 0.40, 2.76, 2.16 and 19.0, respectively. The data on nutrient balances with quails fed diets containing the two-types of DORB suggested no significant (p>0.05) differences in per cent retention of diet dry matter (DM), organic matter (OM), ether extract (EE), gross energy (GE), nitrogen (N), crude fiber (CF), calcium (Ca) or phosphorus (P) content attributable to the extraction process. The age of the birds also appeared to have no significant (p>0.05) effect on nutrient utilization by them. However, the level of inclusion of DORB had a significant (p<0.05) effect on nutrient utilization by quails in that the retention of dietary N, EE, CF, Ca and P was better at lower than at higher levels. It is concluded that the DORB, irrespective of extraction process, can safely be employed upto 20% level in diet of adult Japanese quails without noticeable interference in utilizing the major dietary nutrients.

• Journal of the East Asian Society of Dietary Life
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• v.16 no.6
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• pp.740-746
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• 2006

The proximates, vitamin C, minerals, and fatty acids of five potato cultivars were evaluated by AOAC methods, the hydrazine method, ICP-AES, and gas chromatography, respectively. The proximate analyses; vitamin C, reducing sugar, and soluble solid contents; and mineral and fatty acid compositions were significantly different among the five cultivars. The Superior cultivar contained a higher carbohydrate content and higher Ca/P ratio and lower levels of crude protein and Na. The Atlantic cultivar contained significantly higher amount of energy, carbohydrate, reducing sugar, vitamin C, SEA, and MUFA, and significantly lower amount of minerals and PUFA. The Shepody cultivar contained significantly higher amount of carbohydrate and MUFA, and significantly lower amount of soluble solid, vitamin C, and SFA. In addition, the P, Fe, Mg, Cu, and Al levels were significantly higher in Shepody, and Zn content was significantly lower. The Jopung cultivar contained significantly higher levels of moisture and Na, and significantly lower levels of soluble content, reducing sugar, carbohydrate, crude protein, and fat. Finally, the Namsuh cultivar contained significantly higher amount of soluble solid, crude protein, K, Mg, and Al, and significantly lower amount of reducing sugar.

• Journal of Nutrition and Health
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• v.34 no.8
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• pp.887-895
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• 2001

This study was designed to investigate the effect of dietary boron supplementation and calcium levels on calcium and bone metabolism in ovariectomized female rats. The experimental group classified ovariectomized group(O) and sham operation group (S). The two groups were then each randomly divided into flour sub-groups and fed experimental diets consisting in two levels of calcium and at each level of calcium, there were boron supplemented group and non-supplemented group. Calcium levels were either 0.2%(low calcium group: L) or 1.2%(high calcium group: H). The level of boron in the diet for the boron supplemented groups(B) was 100$\mu\textrm{g}$/g diet. The experimental period was six weeks. The average food intake were not statistically significantly different in all of eight groups. The increase in weights of rats was observed only in ovariectonized and sham control rats(low ca without boron supplemented). The rest of the groups lost weight significantly during the experimental period ranging from 26.94g to 44.34g. Significant higher calcium intakes were observed in high calcium groups, regardless of boron supplementation during experimental period. Urinary calcium excretion was not affected either by ovariectomy or diets on the first, third and sixth week of feeding. Apparent calcium absorption rates were not different among the groups on first week, whereas noticeable increase was observed in low calcium groups at third and sixth weeks. Femur wet and dry weight, and calcium contents of femur were higher in low calcium groups. whereas femur bone density was higher low calcium with boron supplementation groups than low calcium groups. Scapular density did not show any significant differences among all groups. Despite there were no differences in the activities of alkaline phosphatase by boron supplementation, boron supplemetation appeared to cause higher femur density. There results suggest that in both of sham-operated and ovariectomized rats low calcium did not influence greatly bone status of rats and boron increased bone density.

• Journal of Aquaculture
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• v.6 no.2
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• pp.107-123
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• 1993

To define the effects of various levels $(0\~1.5\%)$ of dietary n-3HUFA on the physiological changes in the Korean rockfish, variations in blood variables and hepatocytes were studied. Biochemical serum analyses, lactate dehydrogenase (LDH) activity of the liver cytosol and ATPase activity of the liver microsomal membrane were also studied. The haematological values (red blood cell, hemoglobin, hematocrit, MCHC, MCV and MCH) were not significantly different in the experimental groups $(P\geq0.05)$. The total protein and glucose levels in the serum were affected by dietary n-3HUFA levels. These levels in groups fed n-3HUFA insufficient diets were significantly lower than those of n-3HUFA sufficient groups (P<0.05). Serum levels of total cholesterol, free cholesterol, glutamic pyruvic transaminase (GPT) and glutamic oxaloacetatic transaminase (GOT) showed significantly higher values in the fish fed n-3HUFA deficient diets (P<0.05). The LDH in the serum was dropped with increasing dietary n-3HUFA levels, but the LDH activity of the liver cytosol was elevated. Histologically, the hepatic cell in the fish fed n-3HUFA free diet was abnormal and showed a necrotic condition. $Ca^{2+}-ATPase$ activities of the liver microsomal membrane were significantly lower in the fish fed n-3HUFA deficient diets than in those fed n-3HUFA sufficient diets (p<0.005). These results suggested that the liver cell membrane was affected by dietary fatty acid compositions and cell membrane of the fish fed n-3HUFA insufficient diets showed abnormalities.

• Nutrition Research and Practice
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• v.4 no.5
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• pp.383-392
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• 2010

This study was conducted to prepare basic materials and offer advice regarding dietary habits to prevent and cure childhood obesity by comparing and analyzing dietary habit, nutritional status, blood factors, and mineral contents of hair. All subjects were stratified by their weight-length index (WLI). According to the standard WLI values, 64.9% of children were within the normal value, 13.5% of children were underweight, and 21.6% of children were overweight and obese (WLI ${\geq}$ 110%). Overall, the score assessed dietary habit for all children was $21.32{\pm}2.55$ point (921 subjects), with 5.1% of children having excellent dietary habits and 3.1% having poor dietary habits. Additionally, 37.9% of underweight children, 37.6% of normal weight children, and 43.2% of overweight and obese children consumed higher amounts of protein than underweight children did (meat, fish, eggs, and soy products) (P < 0.05). Overweight and obese children consumed more fried foods than underweight or normal weight children (P < 0.05). Moreover, 38.0% of the children had hemoglobin levels of 12 g/dl, while 7.6% were anemic (11.1 g/dl). When a hematocrit level of 33% was taken as the standard, 11.0% of children were anemic. The plasma transferrin content was $263.76{\pm}54.52$ mg/dl in overweight and obese children. The mean values of Fe, Cu, Ca, Cr, Mn, Se, Na, K, Li, V, Co, and Mo were within the reference values, but the Zn concentrations of underweight, normal weight, and overweight and obese children were $67.97{\pm}28.51$ ppm, $70.09{\pm}30.81$ ppm, and $73.99{\pm}30.36$ ppm, respectively. The Zn concentration of overweight and obese children ($73.99{\pm}30.36$ ppm) was lower than that of the standard value (180~220 ppm). Therefore, a nutritional education program and new guidance for dietary pattern should be developed to reduce the number of underweight and overweight and obese children.

• Journal of Nutrition and Health
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• v.41 no.8
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• pp.786-796
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• 2008

This study examined the influence of the nutrient intake status, dietary habit, and academic stress of elementary school children on their academic achievement. Two-hundred and twenty-four fifth-graders in Bucheon-si, Gyeonggido were surveyed. The study included the 24 hr-recall, anthropometric measurement, assessment of stress level and academic achievement. The subjects were normal in height, weight and Rohrer index, but higher percentage of underweight was seen in girls and vice versa in boys. The overall nutrient intake and dietary habits were fairly good, but Ca and folate intake was less than 75% KDRIs and dietary habits of boys were inferior. Academic stress level of all the subjects was not high. In terms of academic performance and its relations with nutrient intake, the more the amount of nutrient intake, the higher the academic performance. Especially, it was true for the energy, protein, phosphorus, potassium, zinc, polyunsaturated fatty acids, and n-6 fatty acid intakes (p < 0.05). The overall academic performance was higher for those who eating-out less frequently. Children with higher comprehensive dietary habit scores have shown better academic performance (p < 0.05). Less stress implied to those shown higher academic achievement while those with relatively poor academic performance showed high stress level since their grade did not improve as much as they anticipated. In conclusion, the academic achievement was higher for those who have a better nutritional status, better dietary habits, and lower stress levels. Therefore, it is critical for nutritionists, parents, and teachers to improve the nutritional status and dietary habits as well as to help them manage their stress levels, which will eventually contribute to an enhanced academic performance.

• Preventive Nutrition and Food Science
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• v.2 no.3
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• pp.219-224
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• 1997

The pancreas is an important organ in the maintenance of zine homeostasis. The pancreatic tissue used in this study was obtained from rats fed varying levels of dietary Ca nd phytate followed by intraperitoneal {TEX}${65}^Zn${/TEX} injection. THe objective of this study was to determine the molecular size and distribution of compounds that may represent zinc-binding complexes in pancreatic tissue homogenates. The supernatant of the homogenized pancreatic tissue was separated using a Sephadex G-75 column with Tris buffer at pH 8.1. All subfractions were assayed for zinc, protein and {TEX}${65}^Zn${/TEX} activity. The elution of subfractions from pancreatic tissue homogenates showed a prominent peak corresponding to the high molecular weight protein standard (>66kd). A sall molecular weigth protein (<6.5kd), that was absorbed at 280nm, was also present: prominently in low Ca group, however not much as in high Ca group. These small compounds may combine weakly with zinc in pancreatic tissue an serve as zinc-binding ligands in pancreatic/biliary fluid. In the duodenum, these ligands dissociate zinc into an ionic form which becomes vulnerable to phytate complexation.

• Fisheries and Aquatic Sciences
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• v.7 no.1
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• pp.1-9
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• 2004

Sweet smelt (Plecoglossus altivelis) were fed four different diets supplemented with either perilla oil $(2.0\%)$ rich in 18:3n-3 (CP), and perilla oil and Enteromorpha compressa meal $(2.0\%)$ (CPA), soybean oil rich in 18:2n-6 (CO), or soybean oil and algal meal (CA) for 4 weeks. The growth performance, fatty acid composition of muscle, plasma lipid peroxidation and blood components of the sweet smelt were then determined. The specific growth rate and feed efficiency in the fish fed the CPA diet were the highest, while the other groups showed similar results. The fatty acid composition of muscle in sweet smelt reflected the dietary lipids; 18:3n-3 was higher in the fish fed the CP and CPA diets, and 18:2n-6 was higher in the fish fed the CO and CA diets. The other fatty acid profiles presented almost no differences with respect to the diet composition. The fish fed the CA, CP and CPA diets contained significantly lower levels of triglyceride, thiobarbituric acid-reactive substances and hydroxyl radical in their plasma than that fed the CO diet. Phagocytic activity was the highest in the fish fed the CPA diet and higher in those of the fish fed the CP and CA diets compared to the CO diet group. The results from this study suggest that a dietary supplement of $2.0\%$ perilla oil together with $2.0\%$ E. compressa meal may improve the growth and health of cultured sweet smelt.

• Journal of Nutrition and Health
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• v.39 no.2
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• pp.121-132
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• 2006

Minerals are extremely important in human metabolism, growth, and tissue repair. The risks of disturbances in mineral nutrition and metabolism are high following menopause. The aim of the study was to investigate the mineal nutrition status in middle-aged women (Pre & Postmenopausal) and the relation to climacteric symptoms. In the present study, we conducted the anthropometric measurement, climacteric symptoms survey and the dietary intakes including macronutrients, vitamins and minerals to assess the nutrient intakes. Also plasma mineral levels (Ca, P, Mg, Na, K, Fe, Cu, Zn) was measured. The participants were 105 middle-aged women (pre=51, Postmenopausal=54). The anthropometric measurement showed that 74% of subjects was overweight and obesity. The energy intakes were 83% of Korean EER. The macromineral intake (Ca, P, Mg, Na, K), Ca, P and Mg were 65%, > 116% and > 107% of Korean RI. Na and K intakes were > 243% and 53% of Korean AI. For the trace mineral intake (Fe, Cu, Zn), Fe, Cu and Zn intakes were 82%, > 146%, and 94% of Korean RI. The serum mineral concentration measurement, in case of Ca, Fe and Zn, > 10% of subjects showed lower level than normal clinical range. The most frequently appeared climacteric symptoms were dry eye, forgetfulness, difficult concentration, shoulder pain, fatique, depression & lonesomeness, backache, snore, dry skin, ankle & knee pain. In relation to mineral nutrition with climacteric symptoms, significant correlations have been founded between Ca intake and difficult concentration, Mg intake and snore, Fe & Zn intake and forgetfulness & dry skin, serum Ca concentration and forgetfulness, serum Na concentration and dry eyes & snore, serum K concentration and shoulder pain. From these results, it has been indicated that some mineral nutrition status may effect the prevention and reduction of climacteric symptoms in pre and postmenopause women.