• Journal of the Korea Organic Resources Recycling Association
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• v.14 no.3
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• pp.148-156
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• 2006

Potential hydrocarbon degrading bacteria were screened from the site artificially polluted with 20,000 ppm of diesel. Among the isolates, two strains, SJD2 and SJD4, showed higher activities to degrade diesel on the Bushnell-Hass broth medium containing 2% of diesel. 16S rDNA sequence analysis revealed that SJD2 and SJD4 were Bacillus fusifomis and B. cereus, respectively. Both strains were found to grow in a wide range of temperature between $20^{\circ}C-55^{\circ}C$, with the best at $30^{\circ}C-37^{\circ}C$. This is the first report, as far as we know, that B. fusifomis is capable of degrading diesel. We hope that a new isolate, B. fusifomis, will efficiently conduct bioremediation at the contaminated sites with petroleum hydrocarbons.

• Korean Journal of Microbiology
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• v.39 no.2
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• pp.108-113
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• 2003

Diesel oil-degrading bacterial strains were isolated from diesel oil contaminated soil and called HS series (HS1, HS2 and HS3). These strains were identified as Acinetobacter sp. (HS1) and Pseudomonas sp. (HS2 and HS3) based on Biolog test, cellular fatty acid composition, and 16S rDNA sequence analysis. These strains were coltivated in liquid minimal media containing 2% diesel oil, and diesel oil-degrading activity was measured. As result, all strains degraded over 70% of total diesel oil. But PAH (polycyclic aromatic hydrocarbon)- and pris- tane-degrading rate of these strain was below 20％ of total PAH and pristane. The HS 1 strain showed highest hydrophobicity and low emulsifying activity among the experimental strains and high diesel oil-degrading activity. From the above-mentioned result, microcosm experiment was performed with the HS1 strain. The HS1 strain showed a degrading activity of over 80% of total diesel oil in microcosm test. And microbial activity was correlated to diesel oil-degrading activity. Therefore, it is suggested that the HS1 strains could be effectively used for the bioremediation for diesel oil.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2002.04a
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• pp.22-25
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• 2002

The effect of in-situ chemical oxidation on the indigenous soil microorganisms (total microbes and PAH-degrading microbes) and contaminant removal were investigated. Field soil contaminated with diesel in gas station was collected and the soil was treated from 0 to 900 minutes by in-situ ozonation as chemical remediation. The treated soil samples were incubated with supplying oxygen during the 9 weeks to understand the characteristics of microbes regrowth, damaged by ozone. The sharp decrease of aromatic fraction and TPH was observed within 60 minutes of ozone application and aromatic fraction and TPH then slowly decreased. The phenanthren-degrading bacteria were the most sensitive to ozonation, because 1 hour of ozonation reduced the microbes from 10$^{6}$ CFU/g-soil to below detection limits.

• Journal of Soil and Groundwater Environment
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• v.15 no.4
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• pp.13-20
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• 2010

The purpose of this study was to develope the remediation method of contaminated soils with metals and petroleum. The diesel degrading strain was isolated and identified from the soil contaminated by petroleum at industrial sites. Diesel biodegradation experiment was performed by diesel degrading bacteria in both solution and soil slurry. Contaminated soils by Zn or As and diesel were treated consecutively by steam-vapor extraction, biodegradation, and acid washing. The strain was identified as Pseudomonas aeruginosa, and named as Pseudomonas aeruginosa TPH1. The optimal culture conditions of TPH1 were $20^{\circ}C$ and pH 7.0, 3% of diesel concentration. Biodegradation of diesel was performed using the separated strain in liquid medium, and 63% of diesel was degraded in 72 hours. And 52% of diesel was removed in the tested soils. In the treatment of contaminated soils with diesel and Zn or As, 29% ~ 44% of diesel was reduced by steamvapor extraction, 60% ~ 71% of diesel was removed after biodegradation. 47% of Zn and 96% of As were removed after acid(mixture of sulfuric and oxalic acids) washing. It is recommended that consecutive treatment method of steam-vapor extraction, biodegradation and acid washing is effective for remediation of complex contaminated soils with metals and petroleum.