• Design & Manufacturing
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• 제16권3호
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• pp.16-21
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• 2022

In order to reduce the weight of parts and materials for the development of high-efficiency engines in accordance with the strengthening of automobile fuel efficiency regulations, the existing casting material is changed to a iron plate material, and plastic processing and turning operations are performed to lighten the weight and reduce the manufacturing cost. Among the pulley components applied to the damper pulley, the HUB product was manufactured by plastic machining instead of the existing casting process, and the inspection standardized for automating the inner diameter and parallelism measurement of the turning result of the new hub part with improved quality, and the inspection system for this Development of design and operation software to automate the inspection of the inner diameter and parallelism of the hub was described. The representative specifications of the development equipment are a hub inner diameter 22mm inspection system, a three-point inspection system with a parallelism of 0.15mm on the top.

• 대한조선학회논문집
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• 제48권6호
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• pp.528-538
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• 2011

As a forming method for curved hull plates more efficient than the flame bending, mechanical bending using multi point press forming and die-less forming is discussed in this paper. the mechanical forming is a flexible manufacturing system for automatically forming of hull parts. It is especially suited to varied curved parts. This paper discusses a multiple point pressing machine composed of a pair of reconfigurable punches in order to achieve the rapid forming of curved hull plates using division forming and presents how forming information is obtained from the given design surface. Although the mechanical forming can be efficient in the metal forming, spring back after pressing is a phenomenon which must be carefully considered when quantifying the process variables. If the spring back is not accurately controlled, the fabricated shell plate cannot meet assembly tolerance. This paper describes the principles to calculate the proper stroke of each punch at the divided areas. the strokes are determined by an iterative process of sequential pressing and spring back compensation from an unfolded flat shape to its given design surface. FEA(finite element analysis) is used to simulate the spring back of the plate and the IDA(iterative displacement adjustment) method adjusts the offset of pressing punches from the deformation results and the design surface. The shape deviations of two surfaces due to spring back are compensated by integrated system using FEA and IDA method. For the practical application, It is aimed to develop an integrated system that can automatically perform the compensation process and calculate strokes of punches of the double sides' reconfigurable multiple-press machine and some experimental results obtained with mechanical bending are presented.

• Design & Manufacturing
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• 제14권3호
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• pp.8-16
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• 2020

In this study, the vibration signal of the mold was measured and analyzed to monitoring the process time and characteristics during injection molding. A 5 inch light guide plate mold was used to injection molding and the vibration signal was measured by MPU6050 acceleration sensor module attached the surface of fixed mold base. Conditions except for injection speed and packing pressure were set to the same value and the change of the vibration signal of the mold according to injection speed and packing pressure was analyzed. As a result, the vibration signal had a large change at three points: "Injection start", "V/P switchover", and "Packing end". The time difference between "injection start" and "V/P switchover" means the injection time in the injection molding process, and the time difference between "V/P switchover" and "Packing end" means the packing time. When the injection time and packing time obtained from the vibration signal of the mold are compared with the time recorded in the injection molding machine, the error of the injection time was 2.19±0.69% and the error of the packing time was 1.39±0.83%, which was the same level as the actual value. Additionally, the amplitude at the time of "injection start" increased as the injection speed increased. In "V/P switchover", the amplitude tended to be proportional to the pressure difference between the maximum injection pressure and the packing pressure and the amplitude at the "packing end" tended to the pressure difference between the packing pressure and the back pressure. Therefore, based on the result of this study, the injection time and packing time of each cycle can be monitored by measuring the vibration signal of the mold. Also, it was confirmed that the level and trend of process variables such as the injection speed, maximum injection pressure, and packing pressure can be evaluated as the change of the mold vibration during injection molding.

• 한국식품과학회지
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• 제37권5호
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• pp.757-762
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• 2005

압출성형공정을 곡류식품 소재개발에 적용하기 위한 기초연구로서 압출성형으로 곡류를 처리한 후 압출성형 곡류, 원료 곡류, 동결건조 곡류의 수용성지수, 수분흡착지수, 페이스트점도와 미생물지표로서 일반세균수를 측정하였다. 압출성형 시료는 배럴온도(70, 90, $110^{\circ}C$)와 수분함량(25, 30%)을 달리하여 제조하였다. 원료 곡류, 동결건조 곡류, 압출성형 단일곡류와 압출성형 혼합곡류의 수용성지수와 수분흡착지수는 압출성형시료가 원료곡류와 동결건조 곡류에 비해 증가하였다. 압출성형 혼합곡류에서 수분용해지수는 배럴온도 $70^{\circ}C$, 수분함량 25%일 때 가장 높았고 수분흡착지수는 배럴온도 $110^{\circ}C$, 수분함량 25%일 때 가장 높게 나타났다. 원료 곡류, 동결건조 곡류, 압출성형 단일곡류와 압출성형 혼합곡류의 최고점도, 최저점도, 구조파괴점도, 최종점도는 압출성형시료가 원료 곡류와 동결건조 곡류에 비해 현저히 감소하였다. 압출성형 혼합곡류에서 최고점도와 구조파괴점도는 배럴온도 $70^{\circ}C$, 수분함량 25%일 때 가장 낮았고 최저점도는 배럴온도 $110^{\circ}C$, 수분함량 25%일 때 가장 낮은 값을 나타내었다. 최종점도는 배럴온도 $90^{\circ}C$, 수분함량 25%일 때 가장 낮게 나타났다. 미생물지표로서 일반세균수를 측정한 결과는 압출성형 공정으로 처리한 원료곡류와 혼합곡류에서 미생물이 검출되지 않았다.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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• pp.78-78
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• 2003

Embryonic stem (ES) cells, derived from preimplantation embryo, are able to differentiate into various types of cells consisting the whole body, or pluripotency. In contrast, terminally differentiated cells do not usually alter their nature but frequently die or transform if they are exposed to inappropriate external stimulations. In addition to the plasticity, ES cells are expected to be different from terminally differentiated cells in very many ways, such as patterns of gene expressions, ability and response of the cells in confronting environmental stimulations, metabolism, and growth rate. As a model system to differentiate these two types of cells, human ES cells (MB03) and terminally differentiated cells (HeLa), we examined the ability of these two types of cells in confronting a severe oxidative insult, that is $H_2O$$_2$. Approximately 1$\times$10$^4$ cells were plated in 96 well plate and serum starved for overnight. The conditioned cells were exposed to a various concentration of $H_2O$$_2$ fur 24 hrs and loaded with neutral red (50$\mu\textrm{g}$/ml) for 4 hrs, washed with PBS for 2 min three times, and entrapped dye was dissolved out using acetic ethanol. Cytotoxicity was determined by reading the amount of dye in the medium using microplate reader. equipped with 575 nm filter. Relative amount of the dye entrapped within MB03 or HeLa were not significantly different when cells were exposed up to 0.4 mM $H_2O$$_2$. However, this sharply decreased down to 0.12% in HeLa cells when the cells were exposed to 0.8 mM $H_2O$$_2$, while it was approximately 54% in MB03 suggesting that this concentration of $H_2O$$_2$ is the defensive threshold for HeLa cells. The resistance to oxidative stimulation reversed, however, when cells were co-treated with BSO (L-buthionine- 〔S, R〕-sulfoximine) which chelates intracellular GSH. This result suggests that cellular GSH is the major defensive mechanism of human ES cells. Induction of enzymes involved in GSH metabolism and type of cell death is currently being studied.

• Design & Manufacturing
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• 제16권4호
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• pp.24-33
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• 2022

Fiber-reinforced plastics(FRPs) have excellent specific stiffness and strength, so they are usually used as automotive parts that require high rigidity and lightweight instead of metal. However, it is difficult to predict the mechanical properties of injection molded parts due to the fiber orientation and breakage of FRPs. In this paper, the fiber orientation characteristics and mechanical properties of injection molded specimens were evaluated in order to fabricate automotive transmission side covers with FRPs and design a rib structure for improvement of their rigidity. The test molds were designed and manufactured to confirm the fiber orientation characteristics of each position of the injection molded standard plate-shaped specimens, and the tensile properties of the specimens were evaluated according to the injection molding conditions and directions of specimens. A gusset-rib structure was designed to improve the additional structural rigidity of the target products, and a proper rib structure was selected through the flexural tests of the rib-structured specimens. Based on the evaluation of fiber orientation and mechanical characteristics, the optimization analyses of gate location were performed to minimize the warpage of target products. Also, the deformation analyses against the internal pressure of target product were performed to confirm the rigidity improvement by gusset-rib structure. As a result, it could be confirmed that the deformation was reduced by 27~37% compared to the previous model, when the gusset-rib structure was applied to the joining part of the target products.

• 한국환경보건학회지
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• 제25권3호
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• pp.36-43
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• 1999

The present study was performed to investigate biodegradation rate of dichlorvos and methidathion. In the biodegradation test of two pesticides by the modified river die-away method from June 17 to August 22, 1998, the biodegradation rate constants and half-life were determined in Nakdong(A) and Kumho River(B). Biodegradation rate of dichlorvos was 4.51% in A sampling point, 6.88% in B sampling point after 7 days. Biodegradation rate constants and half-life of dichlorvos were 0.0066 and 105 days in A sampling point, 0.0102 and 67.9 days in B sampling point, respectively. Biodegradation rate of methidathion was 23% in A sampling point, 36% in B sampling point after 7 days. Biodegradation rate constants and half-life of methidathion were 0.0377 and 18.4 days in A sampling point, 0.0641 and 10.8 days in B sampling point, respectively. Biodegradation rate of methidathion was faster than that of dichlorvos. This suggested that the difference in biodegradation of pesticides was due to difference in the water quality and standard plate counts in the Nackdong and Kumho Rivers. The result of correlation analysis between biodegradation rate constants of the pesticides and water quality(DO, BOD, SS, ABS, NH$_3$-N, and NO$_3$-N) showed significant correlation with BOD, SS and NH$_3$-N at the 5% significant level. A significant linear equation was obtained from regression analysis at the 5% significant level, whereas, dependent variables were BOD, SS and NH$_3$-N, and the biodegradation rate constant was independent variable. It is suggested that dichlorvos will be mainly degraded by hydrolysis, and for methidathion was both hydrolysis and biodegradation. A significant QSAR equation was obtained from regression analysis at the 10% significant level, whereas, dependent variable is biodegradation rate constants of BPMC, chlorothalonil, dichlorvos and methidathion, vapor pressures, partition coefficients and water solubilities of the pesticides are independent variables. Also, a significant linear equation was obtained from regression analysis at the 1% significant level, whereas, dependent variable is biodegradation rate constants of BPMC, chlorothalonil, dichlorvos and methidathion, hydrolysis rate constants of the pesticides are independent variables. It is suggested that the pesticides will be degraded by main degradation factor when the pesticides was affected both hydrolysis and biodegradation.

• 한국산학기술학회논문지
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• 제19권3호
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• pp.14-20
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• 2018

전기자동차는 가솔린 자동차와는 달리 배출가스가 없어 친환경 차량을 대표하지만, 장착된 축전지에 충전된 전기로 구동되기 때문에, 1회 충전으로 갈 수 있는 거리가 전지의 에너지 밀도에 의해 좌우된다. 따라서 높은 에너지 밀도를 갖는 리튬이온 전지가 전기자동차용 전지로 유력한 후보이다. 리튬이온 전지의 효율을 지배하는 중요한 구성품은 전극이므로 전극 제조공정은 리튬이온 전지 전체 생산 공정에서 중요한 역할을 한다. 특히 전극의 제조 공정 중 코팅 공정은 성능에 큰 영향을 미치는 매우 중요한 공정이다. 본 논문에서는 전극 제조에서 코팅 공법의 효율성 및 생산성 증대를 위한 혁신적인 공정을 제안하고, 장비 설계 방법 및 개발 결과에 대하여 기술하였다. 구체적으로, 극판 핵심 코팅 품질 25% Upgrade 기술, 제품 고출력/고용량화 에 따른 조립 마진 감소 대응 가능 기술, 그리고 제품 용량 품질 및 조립 공정 수율 향상 기술들에 대한 설계 절차 및 개발방법을 제시하였다. 결과로 리튬이온 배터리의 셀의 제품 수명 개선 효과를 확보 하였다. 기존의 코팅 공정과 비교할 때 양극 용량 유지 위해 Target Loading Level 유지, 산포를 향상시켰다(${\pm}0.4{\rightarrow}{\pm}0.3mg/cm^2r$감소).

• 생명과학회지
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• 제17권2호통권82호
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• pp.198-203
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• 2007

형질전환은 유전자의 기능을 이해하는데 매우 중요한 기법이다. $Ca^{2+}$-인산 침전법은 시간과 비용이 저렴하여 가장 흔히 사용된다. 그러나 성숙 신경세포는 어린 신경세포나 다른 세포종에 비하여 형질전환이 어렵고 쉽게 죽는다. 본 연구에서는 Clontech사의 $CalPhos^{TM}$ Mammalian Transfection 방법을 수정하여 성숙한 신경세포를 효율적으로 형질전환할 수 있는 방법을 고안하였다. 대뇌 신경교세포를 DMEM/10% 말혈청에서 70-80% confluence까지 키우고 배지를 혈청이 첨가되지 않은 Neurobasal/Ara-C로 바꾸어 주어 더 이상 신경교세포가 분열하지 않게 한 다음, 여기에 E19 해마신경세포를 접종하여 배양하였다. $DNA/Ca^{2+}$-인산 침전물은 Clontech사의 $CalPhos^{TM}$ Mammalian Transfection Kit을 이용하여 크기($0.5-1\;{\mu}m$ in diameter) 및 농도(약 10 particles/$100\;{\mu}m^2$)를 배지에서 배양시간을 변화시켜 적당히 조절하였다. 이렇게 하면 in vitro에서 2주 이상 배양한 신경세포도 24-well plate 한 well당 10-15개의 형질전환된 건강한 신경세포를 얻을 수 있었다. 이 방법의 효용성을 검증하기 위하여 연접단백질인 $EGFP-CaMKII{\alpha}$ 융합단백질과 RFP 단백질 유전자(각각 $pEGFP-CaMKII{\alpha}$ 및 pDsRed2)를 형질전환한 결과 전자는 점박이 모양, 후자는 세포전체에 퍼진 양상의 표현을 관찰할 수 있었다. 따라서 본 연구는 성숙한 신경세포를 효율적으로 형질전환할 수 있는 방법을 제공한다.