• 생약학회지
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• 제17권2호
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• pp.184-188
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• 1986

The preliminary antifertility test of Dictamnus albus(Rutaceae) showed that a methanol extract of root bark decreased fertility in rats when administered orally on days $1{\sim}10$ postcoitum. Hexane, chloroform, butanol and aqueous fractions from the methanol extract, were tested to produce the result that only the hexane fraction exhibited positive activity. The hexane fraction was subfractionated into acidic, alkaloidal and neutral subtractions and tested. Neutral subtraction showed positive activity. From the neutral subtraction, fraxinellone was isolated as an active principle. The timed-dosing experiments revealed that antifertility activity of fraxinellone appeared to be exerted after tubal exit of the ova to prevent implantations.

• 생약학회지
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• 제28권4호
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• pp.209-214
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• 1997

This study was carried out to find new antitumor agents from plant resource. Three cytotoxic agents were isolated from the root of Dictamnus albus by hexane extraction, silica gel column chromatography and HPLC. They were identified to be dictamnine $(C_{12}H_9NO_2)$, preskimmianine $(C_{17}H_{21}NO_4)$ and fraxinellone $(C_{14}H_{16}O_3)$ on the basis of spectroscopic evidences. In this study, it was newly found that these compounds possess a cytotoxic activity against lung lymphoma L1210 cell line. Among them. Preskimmianine was the most potent against the lymphoma L1210 with a $IC_{50}$ of $3.125\;{\mu}g/ml\;(10.3\;{\mu}M)$. Toxicity of preskimmianine against normal Iymphocyte was observed at the concentration of $50\;{\mu}g/ml\;(165\;{mu}M)$. These results support the pharmacological role of D. albus, a herb known as Paeksun in Korea and used as an anticancer agent in folk medicine.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2008년도 Proceedings of the Convention
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• pp.166-166
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• 2008

• 생약학회지
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• 제16권3호
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• pp.125-128
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• 1985

From the root bark of Dictamus albus (Rutaceae) five furoquinoline alkaloids, robustine, dictamine, ${\gamma}-fagarine$, skimmianine and haplopine were isolated and characterized by spectral data.

• Archives of Pharmacal Research
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• 제29권12호
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• pp.1119-1124
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• 2006

The methanol extract from the aerial parts of Dictamnus albus was active in inhibiting monoamine oxidase (MAO) from the mouse brain. Activity-guided fractionation led to the isolation of four known coumarins, 7-(6'R-hydroxy-3', 7'-dimethyl-2'E, 7'-octadienyloxy) coumarin (1), auraptene (2), umbelliferone (3), and xanthotoxin (4), as active compounds along with an inactive alkaloid, skimmianine (5). Compounds 1 and 2 inhibited MAO activity in a concentration-dependent manner with $IC_{50}$ values of 0.7 and $1.7\;{\mu}M$, respectively. Compounds 1 and 2 showed a slight and potently selective inhibitory effect against MAO-B ($IC_{50}\;0.5\;and\;0.6\;{\mu}M,\;respectively$) compared to MAO-A ($IC_{50}\;1.3\;and\;34.6\;{\mu}M,\;respectively$). According to kinetic analyses derived by Lineweaver-Burk reciprocal plots, compounds 1 and 2 exhibited a competitive inhibition to MAO-B.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2009년도 추계학술대회
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• pp.1087-1090
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• 2009

본 연구는 약용식물로부터 항균활성 물질을 찾기 위하여 Glycyrrhiza uralensis, Dryopteris crassirhizoma, Dictamnus albus, Paeonia lactiflora, Angelica dahurica, Spirodela polyrhiza, Cimicifuga heracleifolia, Bupleurum falcatum, Magnolia kobus, Artemisia princeps, Arctium lappa, Aster tataricus, Hovenia dulcis, Citrus unshiu, Asparagus cochinchinensis, Gardenia jasminoides, Smilax china, Hovenia dulcis, Prunus sargentii, Scutellaria baicalensis등의 약용식물을 사용하였다. 총 20종류의 약용식물로부터 에탄올 추출물을 분리하여 Agrobacterium tumefaciens에 대한 항균활성을 조사하였다. Glycyrrhiza uralensis, Dryopteris crassirhizoma, Cimicifuga heracleifolia, Bupleurum falcatum,의 추출물이 Agrobacterium tumefaciens에 대한 항균활성을 나타냈다.

• Archives of Pharmacal Research
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• 제28권6호
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• pp.675-679
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• 2005

Eight furoquinoline alkaloids were purified from two plants belonging to the Rutaceae family. Kokusaginine. skimmianine, evolitrine, and confusameline were purified from Melicope confusa, and haplopine, robustine, dictamine, and $\gamma$-fagarine from Dictamnus albus. In this study, the eight furoquinoline alkaloids were examined for inhibitory potency against human phos-phodiesterase 5 (hPDE5A) in vitro. DNA encoding the catalytic domain of human PDE5A was amplified from the mRNA of T24 cells by RT-PCR and was fused to GST in an expression vector. GST-tagged PDE5A was then purified by glutathione affinity chromatography and used in inhibition assays. Of the eight alkaloids, $\gamma$-fagarine was the most potent inhibitor of PDE5A, and its single methoxy group at the C-8 position was shown to be critical for inhibitory activity. These results clearly illustrate the relationship between PDE5A inhibition and the methoxy group position in furoquinoline alkaloids.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2008년도 춘계종합학술대회 A
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• pp.980-981
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• 2008

This study was carried out to research antimicrobial agents from medicinal plants, Lonicera japonica, Pinellia ternata, Dictamnus albus, Cryptotympana pustulata, Pinus densiflora, Bupleurum falcatum, Forsythia saxatilis, Castanea crenata, Hovenia dulcis, Prunus sargentii. The ethanol extracts of 10 medicinal plants were tested for the antimicrobial activity against Streptococcus mutans. The extracts of Pinus densiflora showed significant antimicrobial activity against Streptococcus mutans. These results suggested that the extract from Pinus densiflora could be a candidate for new antimicrobial agents against Streptococcus mutans.