• 환경위생공학
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• 제19권1호
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• pp.50-56
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• 2004

Dibutyl phthalate (DBP) is used extensively in the plastic industry and has been known as an endocrine disruptor. Present study was undertaken to examine whether DBP can induce oxidative stress in mice. In this study, oxidative stress was measured in terms of the modification of lipid peroxidation and gamma-glutamyl transferase (GGT) activity. The serum toxicity index, level of lipid peroxidation and triglyceride (TG), and activity of GGT were measured in male ICR mice after a single administration of DBP (5 g/kg, po). DBP did not alter serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine, glucose and cholesterol level. However, the treatment with DBP was found to significantly increase the level of lipid peroxidation in liver and lung. The TG content and activity of GGT in the liver of DBP-exposed animals was also increased. These results indicate that DBP can induce mild oxidative stress in mice. The GGT activity is considered to be increased as one of the adaptive defense mechanisms to oxidative stress induced by DBP.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2003년도 추계학술대회
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• pp.128-128
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• 2003

There are many synthetic chemicals, such as di(2-ethylhexyl) phthalate (DEHP) and dibutyl phthalate (DBP), used in chemical reaction processes in industry. The establishment of toxicity and detection of synthetic chemicals that may pose a genetic hazard in our enviornment is subjects of great concern at present.(omitted)

• 한국환경성돌연변이발암원학회지
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• 제22권4호
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• pp.274-278
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• 2002

Phthalates, suspected endocrine disruptor, are plasticizer and solvent used in industry, and some phthalates are known as potential carcinogen. Most common human exposure to this compounds may occur with contaminated food. It may migrate into food from plastic wrap or may enter food from general environmental contamination, and it has become widespread environmental pollutants, thus leading to a variety of phthalates that possibly threaten the public health. Dibutyl phthalate (DBP) may playa part of cell proliferator, which mediates changes in gene expression and the metabolism of xenobiotics. An understanding of the role of DBP in modulating gene regulation should provide insight regarding mechanisms of DBP induced xenoestrogenic impact. To elucidate the type of genes that are associated with estrogenic activity induced by DBP at the dose (10$^{-8}$ M) appeared proliferating effects, the pattern of gene expression in MCF7 cells was compared between 17$\beta$-estradiol and DBP exposure in the cDNA microarray. From the results, it showed some differences of gene expression patterns between MCF7 cells treated with 17$\beta$-estradiol and DBP, and also DBP shows estrogenic potential with changes in estrogen-related gene expression levels.

• 대한화학회지
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• 제48권1호
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• pp.17-27
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• 2004

SPME-GC/MS법을 사용하여 내분비계 교란물질로 추정되는 플라스틱 가소제류를 동시에 분석할 수 있는 방법을 고안하였다. 본 연구에서 사용한 플라스틱 가소제는 dimethyl phthalate(DMP), diethyl phthalate(DEP), dibutyl phthalate(DBP), benzylbutyl phthalate(BBP), diethylhexyl phthalate(DEHP), di-n-octyl phthalate(DnOP)이었다. 표준 시료에 대한 분석결과에서 검출한계(LOD)는 0.163~0.299 ng/ml, 상대표준편차는(%RSD)는 5.85~15.80이었다. 본 방법을 사용하여 한강, 금강, 낙동강 및 섬진강에서 채취한 시료를 분석한 결과, DBP와 DEHP 만이 4대강 모두에서 미량 검출되었으며, DBP와 DEHP의 농도는 각각 0.192~1.270 ng/ml 및 0.077~1.102 ng/ml 범위를 보였다.

• 한국환경성돌연변이발암원학회:학술대회논문집
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• 한국환경성돌연변이발암원학회 2003년도 추계학술대회
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• pp.128-128
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• 2003

• Safety and Health at Work
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• 제2권1호
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• pp.57-64
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• 2011

Objectives: Although phthalates like dibutyl phthalate (DBP) and di-2-ethylhexyl phthalate (DEHP) are commonly used as plasticizers and their metabolites are especially suspected of reproductive toxicity, little is known about occupational exposure to those phthalates. The aim of this study was to assess the utility of measuring the metabolite concentrations of DBP and DEHP in serum and urine samples as an indicator of occupational exposure to those phthalates. Methods: Phthalate metabolites were analyzed by using column-switching high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS). Results: We detected phthalate metabolites in serum and urine matrices at approximately 10-fold lower than the limit of detection of those metabolites in the same matrix by LC-MS/MS without column switching, which was sufficient to evaluate concentrations of phthalate metabolites for industrial workers and the general population. Conclusion: The accuracy and precision of the analytical method indicate that urinary metabolite determination can be a more acceptable biomarker for studying phthalate exposure and adverse health outcomes.

• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2003년도 추계국제학술대회
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• pp.174-174
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• 2003

There are many synthetic chemicals, such as di(2-ethylhexyl) phthalate (DEHP) and dibutyl phthalate (DBP), used in chemical reaction processes in industry. The establishment of toxicity and detection of synthetic chemicals that may pose a genetic hazard in our enviornment is subjects of great concern at present DEHP, a ubiquitous phthalate plasticizer, induces a wide range of developmental and reproductive toxicities in mammals. DEHP belongs to the large diverse class of peroxisome proliferator compounds, which include herbicides, hypolipidemic drugs. DBP is a plasticizer used to products containing nitrocellulose, polyvinyl acetate, and polyvinyl chloride such as food wraps and blood bags. DBP is also used in cosmetics as a solvent and fixative for perfumes, a suspension agent for solids, an antifoamer, a skin emollient, and hair spray The present study was performed to examine patterns of gene expression in MCF-7 cells following DEHP and DBP exposure. Changes in gene expression were determined by microarray analysis using KISTCHIP-400 including 401 endocrine related genes based on public database and research papers. Of the genes analysis, we determined that genes detected by array showed a 2-fold or greater change in their expression level(increase or decrease). The results of this study demonstrate that a number of genes were differentially expressed in MCF-7 cells but these changes were not significant. Therefore, we keep going this study using microarray analysis and future studies will examine changes of gene expression on time-course and does treatment in variable cell lines.

• Toxicological Research
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• 제17권2호
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• pp.91-96
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• 2001

Phthalates are widely used as plasticizers to impart softness and flexibility to normally rigid polyvinylchloride products. However, there are not much studies jar dermal and ocular irritation toxicity of phthalates. So we investigated the skin or eye irritation effect of some phthalates which was not reported. The primary skin irritation of diethyl phthalate (DEP), diisodecyl phthalate (DIDP), diisononyl phthalate (DINP), dipropyl phthalate (DPP) and dipropyl phthalate (DPrP) was studied. The ocular irritation of dibutyl phthalate(DBP), DIDP, DINP, DPP and DPrP was also studied. DEP, DIDP, DINP, DPP, and DPrP were found to be non-irritating to the skin of the test animals. DBP, DIDP, DINP and DPP were found to be non-irritating to the eye of the rabbits. DPrP caused the slight irritations to the eye in 1 or 2 days after treatment but irritation of the animals was soon recovered.

• 대한화장품학회지
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• 제38권1호
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• pp.33-41
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• 2012

네일락카와 헤어스프레이 같은 화장품에 존재하는 미량의 프탈레이트를 정량분석하기 위하여, 가스크로마토그래피와 질량분석기를 사용한 효과적이면서 환경친화적인 분석방법을 개발하였다. 이들 화장품들은 다량의 유기용매를 함유되어 프탈레이트를 분석하기 위하여 널리 사용되는 시료의 클린업 방법이 적합하지 않았다. 더군다나 미량의 프탈레이트 분석시에는 실험과정 중에서의 오염으로 인해 실제보다 높은 분석값을 산출하게 되는 경우가 매우 많다. 이에 정확한 함량분석 및 이차오염을 방지하기 위해 유기용매를 사용하여 시료를 직접 희석하는 시료 전처리를 적용하였다. 이 분석방법은 높은 정확성, 분석감도, 그리고 시료전처리를 간략히 할 수 있는 이점을 가진다. 화장품에서의 검출되는 빈도가 높고, 사람과 동물에 영향을 미치는 환경호르몬으로 보고되는 dibutyl phthalate (DBP)와 di (2-ethylhexyl) phthalate (DEHP) 두 종의 프탈레이트를 분석대상으로 선정하였다. 정량시 그 정확도 향상을 위해서 내부표준물질로 두 물질의 중수소치환체인 DBP-$d_4$,와 DEHP-$d_4$를 사용하였다. 시험법의 유효화를 시행한 결과 본 시험법이 ppm 농도의 프탈레이트 정량분석에 적합함을 확인하였으며, 네일락카와 헤어스프레이 제품에 약 $25{\mu}g/g$의 농도로 표준물질을 첨가하여 분석한 회수율은 95 - 106.1 % 범위였고, % 상대표준편차 값은 3.9 % 이하였다.

• 멤브레인
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• 제27권4호
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• pp.350-357
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• 2017

본 연구는 열유도 상분리법(thermally induced phase separation, TIPS)을 사용하여, 수처리 분리막에 적용하기 위해, 응고조의 온도 및 열용량의 변화에 따른 분리막의 모폴로지 변화를 관찰하였다. 분리막을 제조하기 위한 소재로는 기계적 물성과 내화학성이 우수한 poly(vinylidene fluoride)(PVDF)와 실리카를 이용하였고, 희석제로는 dioctyl phthalate (DOP), dibutyl phthalate (DBP)를 사용하였다. 다양한 응고액의 열용량 변화에 따른 구조 변화 관찰을 위하여 SEM 이미지를 관찰하였다. 열용량이 증가할수록 PVDF의 결정화 속도가 느려져 큰 기공을 나타내며 열용량이 작을수록 결정화 속도가 증가하여 작은 기공이 생기는 것을 확인하였다.