• 제목/요약/키워드: Diatom analysis

검색결과 107건 처리시간 0.027초

인공기질에서의 부착규조의 초기 군집형성과 천이 (Colonization and Succession of Epilithic Diatoms on Artificial Substrata)

  • 장성현;이정훈;이정호
    • ALGAE
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    • 제21권3호
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    • pp.295-305
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    • 2006
  • The purpose of the study is to understand the ecological characteristics of the early stage of community formation of epilithic diatoms. The samples were daily collected from a site of the Kumho River during 70 days from October 13 to December 21 in 2002. Total 126 diatom taxa collected in the study were classified into 114 species and 12 varieties belonging to 26 genera. About 80% of the taxa collected from the artificial substrata during the whole studying period were observed until tenth day from the start time of the study. In the study the successional process of the diatom community was divided into the three stages of the early, the middle and the later by cluster analysis based on the similarities in species composition among the communities. Cocconeis placentula Ehrenb. and C. placentula var. lineata (Ehrenb.) V.H., horizontally positioned species, would be divided into the early successional species, and Diatoma vulgre Bory, vertically positioned species, was the later successional species. Cell densities and species composition of the diatoms on the artificial substrata were closely similar to them of natural substrata after 40th day from the start time of the study.

Molecular profiling of 18S rRNA reveals seasonal variation and diversity of diatoms community in the Han River, South Korea

  • Muhammad, Buhari Lawan;Lee, Yeon-Su;Ki, Jang-Seu
    • Journal of Species Research
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    • 제10권1호
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    • pp.46-56
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    • 2021
  • Diatoms have been used in examining water quality and environmental change in freshwater systems. Here, we analyzed molecular profiling of seasonal diatoms in the Han River, Korea, using the hypervariable region of 18S V1-V3 rRNA and pyrosequencing. Physicochemical data, such as temperature, DO, pH, and nutrients showed the typical seasonal pattern in a temperate region. In addition, cell counts and chlorophyll-a, were recorded at high levels in spring compared to other seasons, due to the diatom bloom. Metagenomic analysis showed a seasonal variation in the phytoplankton community composition, with diatoms as the most frequently detected in spring (83.8%) and winter (69.7%). Overall, diatom genera such as Stephanodiscus, Navicula, Cyclotella, and Discostella were the most frequent in the samples. However, a large number of unknown Thalassiosirales diatoms were found in spring (35.5%) and winter (36.3%). Our molecular profiling revealed a high number of diatom taxa compared to morphological observation. This is the first study of diatoms in the Han River using molecular approaches, providing a valuable reference for future study on diatoms-basis environmental molecular monitoring and ecology.

Annotation and Expression Profile Analysis of cDNAs from the Antarctic Diatom Chaetoceros neogracile

  • Jung, Gyeong-Seo;Lee, Choul-Gyun;Kang, Sung-Ho;Jin, Eon-Seon
    • Journal of Microbiology and Biotechnology
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    • 제17권8호
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    • pp.1330-1337
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    • 2007
  • To better understand the gene expression of the cold-adapted polar diatom, we conducted a survey of the Chaetoceros neogracile transcriptome by cDNA sequencing and expression of interested cDNAs from the Antarctic diatom. A non-normalized cDNA library was constructed from the C. neogracile, and a total of 2,500 cDNAs were sequenced to generate 1,881 high-quality expressed sequence tags (ESTs) (accession numbers EL620615-EL622495). Based on their clustering, we identified 154 unique clusters comprising 342 ESTs. The remaining 1,540 ESTs did not cluster. The number of unique genes identified in the data set is thus estimated to be 1,694. Taking advantage of various tools and databases, putative functions were assigned to 939 (55.4%) of these genes. Of the remaining 540 (31.9%) unknown sequences, 215 (12.7%) appeared to be C. neogracile-specific since they lacked any significant sequence similarity to any sequence available in the public databases. C. neogracile consisted of a relatively high percentage of genes involved in metabolism, genetic information processing, cellular processes, defense or stress resistance, photosynthesis, structure, and signal transduction. From the ESTs, the expression of these putative C. neogracile genes was investigated: fucoxanthin chlorophyll (chl) a,c-binding protein (FCP), ascorbate peroxidase (ASP), and heat-shock protein 90 (HSP90). The abundance of ASP and HSP90 changed substantially in response to different culture conditions, indicating the possible regulation of these genes in C. neogracile.

Biogenic TiO2 나노입자 전처리가 클로로포름 광분해에 미치는 영향 (Effect of Pretreatment of Biogenic Titanium Dioxide on Photocatalytic Transformation of Chloroform)

  • 권수열;;;김영
    • 한국물환경학회지
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    • 제27권1호
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    • pp.98-103
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    • 2011
  • Photocatalysis using UV light and catalysts is an attractive low temperature and non-energy- intensive method for remediation of a wide range of chemical contaminants like chloroform (CF). Recently development of environmental friendly and sustainable catalytic systems is needed before such catalysts can be routinely applied to large-scale remediation or drinking water treatment. Titanium dioxide is a candidate material, since it is stable, highly reactive, and inexpensive. Diatoms are photosynthetic, single-celled algae that make a microscale silica shell with nano scale features. These diatoms have an ability to biologically fabricate $TiO_2$ nanoparticles into this shell in a process that parallels nanoscale silica mineralization. We cultivated diatoms, metabolically deposited titanium into the shell by using a two-stage photobioreactor and used this biogenic $TiO_2$ to this study. In this study we evaluated how effectively biogenic $TiO_2$ nanoparticles transform CF compared with chemically-synthesized $TiO_2$ nanoparticlesthe and effect of pretreatment of diatom-produced $TiO_2$ nanoparticles on photocatalytic transformation of CF. The rate of CF transformation by diatom-$TiO_2$ particles is a factor of 3 slower than chemically-synthesized one and chloride ion production was also co-related with CF transformation, and 79~91% of CF mineralization was observed in two $TiO_2$ particles. And the period of sonication and mass transfer due to particle size, evaluated by difference of oxygen tention does not affect on the CF transformation. Based on the XRD analysis we conclude that slower CF transformation by diatom-$TiO_2$ might be due to incomplete annealing to the anatase form.

Holocene 中期에 있어서 道垈川流域의 堆積 環境 變化 (The Change of the Depositional Environment on Dodaecheon River Basin during the Middle Holocene)

  • 황상일;윤순옥;조화용
    • 대한지리학회지
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    • 제32권4호
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    • pp.403-420
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    • 1997
  • 道垈川은 牙山灣으로 流入되는 小河川이다. Holocene 中期에 있어서 이 流域盆地의 堆積環境變化를 살펴보기 위하여 boring조사, 硅藻${\cdot}$花粉分析, carbon dating 등을 실시했다. 道垈川 流域의 Holocene 堆積層은 土炭層과 靑灰色 실트層이 互層을 이루고 있으며, 많은 硅藻와 花粉化石을 포함하고 있다. 硅藻와 花粉의 分析 결과에 의하면 靑灰色 실트층이 퇴적될 때는 相對的으로 海進의 경향이 있었고, 土炭層이 퇴적될 때는 相對的인 海面의 安定 내지 海退의 경향이 있었다. 後氷期 海岸線은 약 7,000년 BP 경에 현 도대천 하류부에 도달하였고, 그 후 6,000년 BP 까지 몇 차례 小海進과 海退를 거듯하였다. 약 7,000년 BP 경에 해면(평균고조위)은 약 3m에 이르렀고, 미상승과 하강을 거듭하면서 6,000년 BP 경에 약 5m 까지 上昇하였다. 西海岸에서는 海岸低濕地性 土炭地는 약 7,000년 BP 경에서 3,000년 BP 경까지 주로 형성되었고, 이들의 分布高度는 대부분 현재의 平均海面보다 2~6m 높은 위치에 분포하고 있다. 西海岸 土炭地는 대개 高潮位 海面과 관련된 內灣 鹽生濕地性 土炭地이므로, 潮差가 작은 東海岸의 堤間濕地(swale)에서 형성된 土炭地에 비하여 形成時期가 앞서고 分布高度가 높다.

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Use of Benthic Algae and Bryophytes for Monitoring Rivers

  • Whitton, Brian A.
    • Journal of Ecology and Environment
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    • 제36권1호
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    • pp.95-100
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    • 2013
  • Many countries have adopted a single, well-described approach to the use of phototrophs for monitoring river water quality, which involves the use of indices related to diatom composition at a site. Increasingly these indices have focussed on assessing ambient phosphate concentration. However, there is a wide range of other methods which can provide additional information to make up for any weaknesses in the standard method. Some of these methods are reviewed briefly here. They can be useful, for instance, when considering temporal and spatial variability in phosphate concentration at a particular site and providing much more insight on heavy metal or pesticide pollution than revealed by routine water analysis.

연안 조류에서 Cd와 Zn 독성의 반대 작용 분석 (Analysis of Oppositive Interaction between Cd and Zn Toxicity in Coastal Algae)

  • 이봉헌;김정호;정성옥;김성미;박흥재
    • 한국환경과학회지
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    • 제11권10호
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    • pp.1069-1074
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    • 2002
  • The growth and heavy metal experiments revealed oppositive interactions between toxic metals(Zn and Cd) and Mn when the coastal diatom T. pseudonana were used. Cd and Zn inhibited the algal growth rate only at low Mn ion concentrations and this effect could be accounted for an inhibition of cellular Mn take by the toxic metals. Mn and Zn inhibited cellular Cd take and this indicated a reciprocal effects among the metals with respect to metal take. Saturation kinetics modeling of the take data was consistent with two metals competing with each other for binding to the Mn take system and with both Cd and Mn being transported into the cell by that system. Mathematical modeling of Mn and Cd take data revealed evidence fur a Cd efflux system.

한반도 하구역 부착돌말류의 공간적 분포 (Spatial Distribution of Epilithic Diatom Communities in the Estuary of Korean Peninsula)

  • 김하경;조인환;김영효;이민혁;김용재;원두희;황수옥;변정환;황순진;김백호
    • 생태와환경
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    • 제51권1호
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    • pp.1-15
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    • 2018
  • With land-use (cover) and water quality, the distributional characteristics of epilithic diatom communities were studied with 193 samples from estuaries of Korean peninsula between 2015 and 2016. Of total 394 taxa classified, Nitzschia perminuta (19.6%) and N. inconspicua (14.0%) were the 1st and 2nd dominant species. Using a cluster analysis, the epilithic diatom communities of Korean estuaries were divided into four groups (G1-G4). Ecological characteristics of each group were followed: G1 was located in estuaries of the East Sea, and characterized by high forest land-use and high DO and low nutrients; G2 was the eastern part of the South Sea, and characterized by low turbidity and nutrients; G3 was the western part of the South Sea, and characterized by high agriculture, low electric conductivity and low salinity; G4 was the Yellow Sea, and characterized by high nutrients. The environmental factors having significant correlation with diatom distributions were as follows: TN to G1, turbidity to G2, agriculture to G3, and TP to G4. Moreover, the important factors affecting the occurrence of indicator species were forest land-use for Fragilaria construens var. venter in G1, turbidity for Rhoicosphenia abbreviata in G2, urban land- use and total phosphorus (TP) for Bacillaria paradoxa and Hantzschia amphioxys of G3, and TP and turbidity for N. ovalis and Stephanodiscus invistatus of G4. These results collectively indicate that the distribution of epilithic diatom communities in Korean peninsula was largely effected by water quality and land cover/use.

울산시 태화강 하류부의 Holocene 중기 이래 해수면변동과 고지형변화 (Sea level change and paleogeomorphological change since the middle Holocene in the lowerreaches of Taehwa River, Ulsan-si, South Korea)

  • 김정윤;황상일;윤순옥
    • 한국지형학회지
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    • 제23권1호
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    • pp.61-75
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    • 2016
  • This study aims to reconstruct sea level change and paleogeomorphology since the middle Holocene by diatom analysis and 14C-dating from the sedimentary facies of three trenches on alluvial plain of Taehwa River, Taehwa-dong, Ulsan-si, South Korea. The Taewha-dong area was a narrow bay located between Bangeojin and eatuary of Taehwa River was a narrow bay which has been detached from the open sea during middle Holocene. Taewa-dong area at bay area was developed into alluvial plain by the sediments had been come from the Taewa river basin. The sea level change during the middle Holocene effected on the development of Taewha-dong alluvial plain and its information is included in the sediment facies.