• Biomolecules & Therapeutics
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• v.24 no.3
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• pp.298-304
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• 2016

Dianthus superbus (D. superbus) is a traditional crude drug used for the treatment of urethritis, carbuncles and carcinomas. The objective of this study was to confirm the cognitive enhancing effect of D. superbus in memory impairment induced mice and to elucidate the possible potential mechanism. Effect of D. superbus on scopolamine induced memory impairment on mice was evaluated using the Morris water maze and passive avoidance tests. We also investigated acetylcholinesterase (AChE) activity and brain-derived neurotropic factor (BDNF) expression in scopolamine-induced mice. HPLC-DAD analysis was performed to identify active compounds in D. superbus. The results revealed that D. superbus attenuated the learning and memory impairment induced by scopolamine. D. superbus also inhibited AChE levels in the hippocampi of the scopolamine-injected mice. Moreover, D. superbus increased BDNF expression in the hippocampus. Eight compounds were identified using HPLC-DAD analysis. The content of 4-hydroxyphenyl acetic acid was higher than contents of other compounds. These results indicated that D. superbus improved memory functioning accompanied by inhibition of AChE and upregulation of BDNF, suggesting that D. superbus may be a useful therapeutic agent for the prevention or treatment of Alzheimer's disease.

• Korean Journal of Pharmacognosy
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• v.30 no.3
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• pp.320-327
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• 1999

'Gu Maig(瞿麥)' is one of the Chinese crude drugs used mainly to cure a diuretic, gonorhoea and menostasis, etc. With regard to the botanical origin of 'Gu Maig', it has been considered to be Dianthus spp. of Caryophyllaceae, but there has not been studied pharmacognostically. To clarify the botanical origin of the "Gu Maig", the morphological and anatomical characteristics of the leaves and stems of Dianthus species growing in Korea, i.e. D. sinensis, D. superbus var. longicalycinus, D. repens, D. morii, D. superbus var. speciosus were compared. As a result, it was determined that 'Gu Maig' was the whole plant body of Dianthus sinensis and D. superbus var. longicalycinus.

• Journal of Physiology & Pathology in Korean Medicine
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• v.24 no.5
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• pp.854-858
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• 2010

Streptococcus mutans (S. mutans) plays an important role in the information of dental plaque and it is being noticed as major causative bacteria of dental caries. In the present study, inhibitory effects of the ethanol extract of Dianthus superbus Linne (D. superbus) on the growth, acid production, adhesion and water-insoluble glucan synthesis of S. mutans were examined. The ethanol extract of D. superbus (0.5 - 4 mg/ml) significantly lowered the growth of S. mutans in a dose dependent manner. The acid production of S. mutans were inhibited by the presence of ethanol extract of D. superbus(1 - 4 mg/ml) significantly. The ethanol extract of D. superbus (0.25 - 4 mg/ml) also significantly lowered the adherence of S. mutans in a dose dependent manner. In water-insoluble glucan synthesis assay, 0.25 - 4 mg/ml of the ethanol extract of D. superbus significantly inhibited the formation of water-insoluble glucan. These results suggest that D. superbus may inhibit the caries-inducing properties of S. mutans. Further studies are necessary to clarify the active constituents of D. superbus responsible for such biomolecular activities.

• The Journal of the Korean Society for Microbiology
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• v.22 no.2
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• pp.185-193
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• 1987

Ten species of herbae, which have been used to treat cancers in Chinese medicine, were tested to investigate their mutagenicity or antimutagenicity in S. typhimurium TA97, TA98, TA100, TA1535, and TA1538. Scolopendra centipede was weakly active in reversion of the frameshift mutation in S. typhimurium TA97 strain and the base-pair substitution in TA100 and TA1535 strains. Other herbae such as Coix lachryma, Dianthus superbus, Tricanthoshse kirilowii, Eupatorium formosanum, Lithospermum erythrorhizon, Ansaema japonicum, Curcuma zedoaria, Helicteres angustifolia, and Euonymus sieboldianus did not show any of the mutagenic potential, regardless of the metabolic activation with rat hepatic microsomal fraction. Dianthus superbus, Eupatorium formosanum, and Euonymus sieboldianus exhibited suppressive activities on microbial mutagenesis of N-methyl-N'-nitrosoguanidine, a base-pair substitution mutagen, in TA1535 and TA100 tester strains. The antimutagenic activities of Dianthus superbus and Euonymus sieboldianus appeared to be dose-dependant.

• Journal of Plant Biology
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• v.37 no.3
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• pp.381-385
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• 1994

The highest degree of callus formation was obtained from the shoot tips of Dianthus superbus when cultured on the MS medium supplemented with 2.0 mg/L NAA and 0.5 mg/L BAP. Embryogenic calluses were obtained from the seperated friable calluses on MS medium containing 2.0 mg/L 2,4-D after 7-8 wk of culture. For plant regeneration, embryogenic calluses were selected and cultured on te proliferation medium. After 3 wk, somatic embryos appeared on MSK medium (0.5 mg/L NAA, 2.0 mg/L kinetin) and N6 medium (2.0 mg/L kinetin, 0.1 mg/LNAA, 0.1 mg/L 2,4-D and 2.0 g/L casein hydrolysate). When these somatic embryos were kept under continuous illumination, shoots were successfully regenerated on the both media. The shoots were rooted on MS medium supplemented with 2.0 mg/L NAA.

• Korean Journal of Plant Tissue Culture
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• v.22 no.1
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• pp.41-46
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• 1995

Leaf mesophyll protoplasts of Dianthus superbus were cultured in MSP1 liquid medium supplemented with 0.5 mg/L BAP, 2.0 mg/L NAA and 9% mannitol. Protoplast-derived colonies were formed after 3 to 4 weeks of culture in the dark at 27$^{\circ}C$. These colonies were kept under continuous illumination (21.5 $\mu$E. m-2 sec-1) for 2 weeks and finally most of the colonies became green microcalli, about 3 mm in diameter. When green microcalli were transferred to MS solidified medium with 2.0 mg/L 2,4-D, they formed embryogenic calli after 4 week of culture. These calli were then transferred onto $N_{6}$ medium containing 0.1mg/L 2,4-D, 0.1 mg/L NAA, 2.0 mg/L kinetin and 2.0 g/L casein hydrolysate and cultured under illumination. After 5 weeks of culture the calli gave rise to multiple shoots of 10 to 15 per callus. Upon transfer onto MS medium containing 2.0 mg/L NAA, they were noted. The regenerates were successfully transplanted into potting soil.

• Proceedings of the KSCN Conference
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• 2005.10a
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• pp.84-84
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• 2005

• Proceedings of the Plant Resources Society of Korea Conference
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• 2007.11a
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• pp.353.2-353.2
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• 2007

• Journal of the Korean Society of Environmental Restoration Technology
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• v.8 no.1
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• pp.10-16
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• 2005

This experiment was conducted to study on application of several Korean native plants by seed spray methods for cut slope revegetation, and possibility of replacement almost imported tall fescue seeds by native herbaceous plants. So, we investigated growth and covering rate after sowing native plants seeds at the artificial slope plots in Suwon and the rock exposed cut-slopes in Wonju city. Emergence rate after seed spray at artificial slopes were higher Elsholtzia splendens and Dianthus superbus var. longicalycinus, showing the highest in E. splendens. Also, E. splendens, D. superbus var. longicalycinus, and Agrostemma coronaria were possible to use for seed spray at the rock exposed cut-slopes. The plots of mixed native plants show more seasonal scenery than that of tall fescue. Soil surface run-off by Typhoon was less in plot sown native plants than those of lawn grass, resulting fresh weight of roots was heavier. Thus, we found that the mixed seed spray of several native herbaceous plants, E. splendens, D. superbus var. longicalycinus, and Agrostemma coronaria, were well covered the slopes as tall fescue.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.8
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• pp.5070-5077
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• 2014

Many countries in the world have protected their native plants and utilized them as industrial materials in each country. In this aspect, it is increasingly important to develop cosmetics materials using native plants in Korea. Cosmetic materials have been developed with niche plants, such as Campanula takesimana Nakai, Dianthus superbus, Aster spathulifolius in Ullengdo, in which a specific plant distribution by distinct climate and environment was present. Water and ethanol extractions were performed from the calluses of Campanula takesimana Nakai, Dianthus superbus, Aster spathulifolius. HPLC analysis revealed different compositions and functions of effective elements in each ethanol extract. For example, all types of ethanol extracts showed an ability to heal wounds. In particular, the expression of the inflammation-related gene, COX-2, was decreased in response to the ethanol extracts of Dianthus superbus. These results indicate that the ethanol extracts from niche plants' calluses in Ullengdo are natural and environmentally-friendly compounds, and can be used as medical supplies associated with anti-inflammation and wound healing.