• 제목/요약/키워드: Diagnosis Instrument

검색결과 191건 처리시간 0.037초

기능성 소화불량 한의 변증 표준화를 위한 이중탕, 평위산 및 시호소간탕 투여 : 무작위 배정, 평가자 눈가림, 3군 비교, 평행 설계, 공개, 다기관 임상시험 프로토콜 (Administration of Yijung-tang, Pyeongwi-san, and Shihosogan-tang for Standardization of Korean Medicine Pattern Identification for Functional Dyspepsia: A Study Protocol of a Randomized, Assessor-blind, 3-Arm, Parallel, Open-label, Multicenter Clinical Trial)

  • 이보람;조민진;최영은;권오진;임미영;고석재;김소연;김용주;남동현;최동준;이준환;박재우;김호준
    • 대한한방내과학회지
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    • 제43권6호
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    • pp.1105-1121
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    • 2022
  • Objectives: The purpose of this study is to explore the effectiveness and safety of frequently used clinical herbal medicines (Yijung-tang [Lizhong-tang, LJT], Pyeongwi-san [Pingwei-san, PWS], and Shihosogan-tang [Chaihu Shugan-tang, SST]) in patients with functional dyspepsia (FD) when administered according to herbal medicine and Korean medicine pattern identification. The results of this study will be used to standardize the diagnostic instrument used in Korean medicine and to investigate biomarkers of Korean medicine pattern identification. Methods: This study will be a randomized, assessor-blind, 3-arm, parallel, open-label, multi-center clinical trial. A total of 300 FD participants will be recruited from 3 Korean medical hospitals and assigned to the LJT (n=100), PWS (n=100), and SST (n=100) groups according to FD pattern identification. The patients will take the medication for 8 weeks, 3 times a day, before or between meals. The primary outcome will be total dyspepsia symptom (TDS) and the secondary outcomes will be adequate relief (AR) for dyspepsia, overall treatment effect (OTE), visual analogue scale (VAS), functional dyspepsia-related quality of life (FD-QoL), gastrointestinal symptom score (GIS), and pattern identification questionnaires. For the exploratory outcomes, we will analyze blood and fecal metabolome profiles, microbiota from fecal and saliva samples, single nucleotide polymorphism (SNP), and results of Korean medicine diagnosis device measurements (heart rate variability, and tongue, pulse, and abdominal diagnosis). Conclusions: The results of this study will prove objectivity for Korean medicine pattern identifications, and the effectiveness and safety of herbal medicines for the population with FD.

집적형 이온선택성 미세전극 센서에 적합한 토양화학 분석용 침출액 종 개발 (Development of Extracting Solution for Soil Chemical Analysis Suitable to Integrated Ion-selective Micro-electrodes)

  • 신국식;임우진;이상은;이재선;차근식
    • 한국토양비료학회지
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    • 제42권6호
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    • pp.513-521
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    • 2009
  • 본 연구는 현장에서 신속히 토양양분을 측정하기에 적합한 집적형 미세 이온선택성 전극을 토양화학성 분석에 이용하기 위하여, 이에 적합한 침출액의 종류와 양과 같은 분석방법을 개발 하는 데에 목적을 두었다. 대상 토양화학성들은 교환성 양이온들($K^+$, $Ca^{2+}$, $Mg^{2+}$, $Na^+$)과 무기태 질소들($NH_4{^+}$, $NO_3{^-}$) 이었으며, 분석법 개발의 목표는 다 성분 동시침출액 종 선발에 있었다. 침출액 자체에 존재하는 화학종들은 이온간 방해작용으로 이온선택성 전극의 분석능에 영향을 미친다. 순수 용액 내에서는 0.01M HCl 과 1M LiCl 이 모든 분석대상 화학종과 그들의 존재 농도범위($10^{-1}M{\sim}10^{-4}M$)에 대하여 가장 Nernst 이론값에 근접하였다. 그러나 실제 토양 침출용액에서 1M LiCl은 고농도(1M)의 $Li^{+}$ 존재로 말미암아 분석대상 화학성분의 선택성이 현저히 낮아짐을 알 수 있었다. 반면에, 0.1M HCl로 침출하여 10배 희석 측정하거나 또는 0.01M HCl로 직접 침출하여 측정하는 것은 표준분석 방법과 고도의 유의성이 있는 상관관계를 보이므로 최적 분석 방법으로 밝혀졌다. 토양에 대하여 집적형 이온선택성 미세 전극을 사용한 분석치와 표준분석법 분석치의 사이의 회귀상관에서는 $K^+$, $Na^+$, $Ca^{2+}$, $NO_3{^-}$가 매우 우수한 회귀상관 관계를 보였다. 그러나 $NH_4{^+}$이온은 $K^+$이온과 혼재할 때 $K^+$이온의 간섭으로 매우 낮은 선택성을 나타내었다. 또한, $Mg^{2+}$이온은 현재까지 이온선택성 막을 위한 최적의 Ionophore(이온투과 담체)가 개발되어 있지 않아 분석의 어려움이 있었다.

조기퇴원 제왕절개 산욕부를 위한 가정간호 표준서 개발 (Development of validated Nursing Interventions for Home Health Care to Women who have had a Caesarian Delivery)

  • 황보수자
    • 간호행정학회지
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    • 제6권1호
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    • pp.135-146
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    • 2000
  • The purpose of this study was to develope, based on the Nursing Intervention Classification (NIC) system. a set of standardized nursing interventions which had been validated. and their associated activities. for use with nursing diagnoses related to home health care for women who have had a caesarian delivery and for their newborn babies. This descriptive study for instrument development had three phases: first. selection of nursing diagnoses. second, validation of the preliminary home health care interventions. and third, application of the home care interventions. In the first phases, diagnoses from 30 nursing records of clients of the home health care agency at P. medical center who were seen between April 21 and July 30. 1998. and from 5 textbooks were examined. Ten nursing diagnoses were selected through a comparison with the NANDA (North American Nursing Diagnosis Association) classification In the second phase. using the selected diagnoses. the nursing interventions were defined from the diagnoses-intervention linkage lists along with associated activities for each intervention list in NIC. To develope the preliminary interventions five-rounds of expertise tests were done. During the first four rounds. 5 experts in clinical nursing participated. and for the final content validity test of the preliminary interventions. 13 experts participated using the Fehring's Delphi technique. The expert group evaluated and defined the set of preliminary nursing interventions. In the third phases, clinical tests were held at in a home health care setting with two home health care nurses using the preliminary intervention list as a questionnaire. Thirty clients referred to the home health care agency at P. medical center between October 1998 and March 1999 were the subjects for this phase. Each of the activities were tested using dichotomous question method. The results of the study are as follows: 1. For the ten nursing diagnoses. 63 appropriate interventions were selected from 369 diagnoses interventions links in NlC., and from 1.465 associated nursing activities. From the 63 interventions. the nurses expert group developed 18 interventions and 258 activities as the preliminary intervention list through a five-round validity test 2. For the fifth content validity test using Fehring's model for determining lCV (Intervention Content Validity), a five point Likert scale was used with values converted to weights as follows: 1=0.0. 2=0.25. 3=0.50. 4=0.75. 5=1.0. Activities of less than O.50 were to be deleted. The range of ICV scores for the nursing diagnoses was 0.95-0.66. for the nursing interventions. 0.98-0.77 and for the nursing activities, 0.95-0.85. By Fehring's method. all of these were included in the preliminary intervention list. 3. Using a questionnaire format for the preliminary intervention list. clinical application tests were done. To define nursing diagnoses. home health care nurses applied each nursing diagnoses to every client. and it was found that 13 were most frequently used of 400 times diagnoses were used. Therefore. 13 nursing diagnoses were defined as validated nursing diagnoses. Ten were the same as from the nursing records and textbooks and three were new from the clinical application. The final list included 'Anxiety', 'Aspiration. risk for'. 'Infant behavior, potential for enhanced, organized'. 'Infant feeding pattern. ineffective'. 'Infection'. 'Knowledge deficit'. 'Nutrition, less than body requirements. altered', 'Pain'. 'Parenting'. 'Skin integrity. risk for. impared' and 'Risk for activity intolerance'. 'Self-esteem disturbance', 'Sleep pattern disturbance' 4. In all. there were 19 interventions. 18 preliminary nursing interventions and one more intervention added from the clinical setting. 'Body image enhancement'. For 265 associated nursing activities. clinical application tests were also done. The intervention rate of 19 interventions was from 81.6% to 100%, so all 19 interventions were in c1uded in the validated intervention set. From the 265 nursing activities. 261(98.5%) were accepted and four activities were deleted. those with an implimentation rate of less than 50%. 5. In conclusion. 13 diagnoses. 19 interventions and 261 activities were validated for the final validated nursing intervention set.

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비외상성 피질하 뇌혈관 질환 환자에서 인지기능, 정신행동 증상 및 일상 생활 기능간의 상관에 대한 연구 (A Study on Correlations Among Cognitive Functions, Neurobehavioral Symptoms and Daily Living Functions in Patients with Non-Traumatic Subcortical Cerebrovascular Disease)

  • 이영호;박영수;최홍;최영희;고대관;정영조;박병관;김수지;정숙희;고병희;송일병;박건우;이대희
    • 정신신체의학
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    • 제4권2호
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    • pp.170-181
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    • 1996
  • Objective : This study was tried to investigate the specific relationships among cognitve function, neurbehavioral symptoms, and daily living functions, as well as provide the guidline of more proper clinical approches for patients with subcortical cerebrovascular disease. Objects and Methods Subjects were 85 patients whose diagnosis was confirmed by brain CT or MRI and controls were 195 normal persons matched by educational level with the subjects. The cognitive functions were evaluated by BNA(Benton neuropsychiatric assessment), subjective neurobehavioral symptoms by SCL-90-R(Sympton Check List-90-Revised), objective neurobehavioral symptoms by NRS(Neurobehavioral Rating Scale), and daily living function symptoms by NRS(Neurobehavioral Rating Scale), and daily living function by GERRI(Geriatric Evaluation by Relative's Rating Instrument) and IADL(Instrumental Activities of Daily Living Scale). Results: 1) Subjects showed significantly lower cognitive functions than controls in all tests of BNA except Lt-Rt Orientation Test(p=0.09) and facial Recognition Test(p=0.186). 2) In subjective neurobehavioral symptoms, subjects showed significantly lower scores in all symptoms except anxiety(p=0.059), hostility(p=0.159), and phobic anxiety(p=0.849). But in objects neurobehavioral symptoms, subjects showed significantly higher in scores in psychoticism (p=0.000) and neuroticism(p=0.025) of NRS. 3) The score of social functioning of GERRI(p=0.000) and that of IADL(p=0.000) were significantly higher in subjects than in controls. 4) for correlation between cognitive and daily living functions, there were significant correlations between the scores of all items on BNA and the score of cognitive or social function of GERRI and the socre of MDL in corntrols, whereas in subjects, there were significant correlations only between the scores of BNA and the score of IADL. 5) for correlation between neuroehavioral symptoms and daily living functions, there were significant correlatons between the socre of subjective neurobehavioral symptoms and the scores of all subscales of GERRI and the score of MDL in controls. On the contrary, in subjects, there were significant correlations between the score of social function of GERRI and the score of objective neurobehavioral symptoms such as psychoticism, agitiation-hostility, and decrease d motivation-emotional withdrawl. Conclusion : Above results suggest that disturbances in specific function of brain may play a role as a predictor of impairments with specific daily living functions and also suggest that specific correlations among various functions may be useful as clinical parameters for setting of the treatment goal and for assessing the ongoing process in the treatment and rehavilitation of the patients with subcortical cerebrovascular disease.

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THE CURRENT STATUS OF BIOMEDICAL ENGINEERING IN THE USA

  • Webster, John G.
    • 대한의용생체공학회:학술대회논문집
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    • 대한의용생체공학회 1992년도 춘계학술대회
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    • pp.27-47
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    • 1992
  • Engineers have developed new instruments that aid in diagnosis and therapy Ultrasonic imaging has provided a nondamaging method of imaging internal organs. A complex transducer emits ultrasonic waves at many angles and reconstructs a map of internal anatomy and also velocities of blood in vessels. Fast computed tomography permits reconstruction of the 3-dimensional anatomy and perfusion of the heart at 20-Hz rates. Positron emission tomography uses certain isotopes that produce positrons that react with electrons to simultaneously emit two gamma rays in opposite directions. It locates the region of origin by using a ring of discrete scintillation detectors, each in electronic coincidence with an opposing detector. In magnetic resonance imaging, the patient is placed in a very strong magnetic field. The precessing of the hydrogen atoms is perturbed by an interrogating field to yield two-dimensional images of soft tissue having exceptional clarity. As an alternative to radiology image processing, film archiving, and retrieval, picture archiving and communication systems (PACS) are being implemented. Images from computed radiography, magnetic resonance imaging (MRI), nuclear medicine, and ultrasound are digitized, transmitted, and stored in computers for retrieval at distributed work stations. In electrical impedance tomography, electrodes are placed around the thorax. 50-kHz current is injected between two electrodes and voltages are measured on all other electrodes. A computer processes the data to yield an image of the resistivity of a 2-dimensional slice of the thorax. During fetal monitoring, a corkscrew electrode is screwed into the fetal scalp to measure the fetal electrocardiogram. Correlations with uterine contractions yield information on the status of the fetus during delivery To measure cardiac output by thermodilution, cold saline is injected into the right atrium. A thermistor in the right pulmonary artery yields temperature measurements, from which we can calculate cardiac output. In impedance cardiography, we measure the changes in electrical impedance as the heart ejects blood into the arteries. Motion artifacts are large, so signal averaging is useful during monitoring. An intraarterial blood gas monitoring system permits monitoring in real time. Light is sent down optical fibers inserted into the radial artery, where it is absorbed by dyes, which reemit the light at a different wavelength. The emitted light travels up optical fibers where an external instrument determines O2, CO2, and pH. Therapeutic devices include the electrosurgical unit. A high-frequency electric arc is drawn between the knife and the tissue. The arc cuts and the heat coagulates, thus preventing blood loss. Hyperthermia has demonstrated antitumor effects in patients in whom all conventional modes of therapy have failed. Methods of raising tumor temperature include focused ultrasound, radio-frequency power through needles, or microwaves. When the heart stops pumping, we use the defibrillator to restore normal pumping. A brief, high-current pulse through the heart synchronizes all cardiac fibers to restore normal rhythm. When the cardiac rhythm is too slow, we implant the cardiac pacemaker. An electrode within the heart stimulates the cardiac muscle to contract at the normal rate. When the cardiac valves are narrowed or leak, we implant an artificial valve. Silicone rubber and Teflon are used for biocompatibility. Artificial hearts powered by pneumatic hoses have been implanted in humans. However, the quality of life gradually degrades, and death ensues. When kidney stones develop, lithotripsy is used. A spark creates a pressure wave, which is focused on the stone and fragments it. The pieces pass out normally. When kidneys fail, the blood is cleansed during hemodialysis. Urea passes through a porous membrane to a dialysate bath to lower its concentration in the blood. The blind are able to read by scanning the Optacon with their fingertips. A camera scans letters and converts them to an array of vibrating pins. The deaf are able to hear using a cochlear implant. A microphone detects sound and divides it into frequency bands. 22 electrodes within the cochlea stimulate the acoustic the acoustic nerve to provide sound patterns. For those who have lost muscle function in the limbs, researchers are implanting electrodes to stimulate the muscle. Sensors in the legs and arms feed back signals to a computer that coordinates the stimulators to provide limb motion. For those with high spinal cord injury, a puff and sip switch can control a computer and permit the disabled person operate the computer and communicate with the outside world.

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호흡기능장애와 관련된 간호진단의 타당도 조사 (Validity of Nursing Diagnoses Related to Difficulty in Respiratory Function)

  • 김조자;이원희;유지수;허혜경;김창희;홍성경
    • 대한간호학회지
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    • 제23권4호
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    • pp.569-584
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    • 1993
  • This study was done to verify validity of nursing diagnoses related to difficulty in respiratory function. First, content validity was examined by an expert group considering the etiology and the signs / symptoms of three nursing diagnoses - ineffective airway clearance, ineffective breathing pattern, impaired gas exchange. Second, clinical validity was examined by comparing the frequencies of the etiologies and signs / symptoms of the three nursing diagnoses in clinical case studies with the results of the content validity. This study was a descriptive study. The sample consisted of 23 experts (professors, head nurses and clinical instructors) who had had a variety of experiences using nursing diagnoses in clinical practice, and 102 case reports done by senior student nurses of the college of nursing of Y-university. These reports were part of their clinical practice in the ICU. The instrument used for this study was a checklist for etiologies and signs and symptoms based on the literature, Doenges and Moorhouse (1988), Kim, McFarland, McLane (1991), Lee Won Hee et al. (1987), Kim Cho Ja et at. (1988). The data was collected over four month period from May 1992 to Aug. 1992. Data were analyzed using frequencies done with the SPSS / PC+ package. The results of this study are summarized as follows : 1. General Characteristics of the Expert Group A bachelor degree was held by 43.5% and a master or doctoral degree by 56.5% of the expert group. The average age of the expert group was 35.3 years. Their average clinical experience was 9.3 years and their average experience in clinical practice was 5.9 years. The general characteristics of the patients showed that there were more women than men, that the age range was from 1 to over 80. Most of their medical diagnoses were diagnoses related to the respiratory. system, circulation or neurologic system, and 50% or more of them had a ventilator with intubation or a tracheostomy. The number of cases for each nursing diagnoses was : · Ineffective airway clearance, 92 cases. · Ineffective breathing pattern, 18 cases. · Impaired gas exchange, 22 cases. 2. The opinion of the expert group as to the classification of the etiology, and signs and symptoms of the three nursing diagnoses was as follows : · In 31.8% of the cases the classification of etiology was clear. · In 22.7%, the classification of signs and symptoms was clear. · In 17.4%, the classification of nursing interventions was clear. 3. In the expert group 80% or mere agreed to ‘dysp-nea’as a common sign and symptom of the three nursing diagnoses. The distinguishing signs and symptoms of (Ineffective airway clearance) were ‘sputum’, ‘cough’, ‘abnormal respiratory sounds : rales’. The distinguishing sings and symptoms of (Ineffective breathing pattern) were ‘tachypnea’, ‘use of accessory muscle of respiration’, ‘orthopnea’ and for (Impaired gas exchange) it was ‘abnormal arterial blood gas’, 4. The distribution of etiology, and signs and symptoms of the three nursing diagnoses was as follows : · There was a high frequency of ‘increased secretion from the bronchus and trachea’ in both the expert group and the case reports as the etiology of ineffective airway clearance. · For the etiologies for ineffective breathing pat-tern, ‘rain’, ‘anxiety’, ‘fear’, ‘obstructions of the tract, ca and bronchus’ had a high ratio in the ex-pert group and ‘decreased expansion of lung’ in the case reports. · For the etiologies for impaired gas exchanges, ‘altered oxygen -carrying capacity of the blood’ and ‘excess accumulation of interstitial fluid in lung’ had a high ratio in the expert group and ‘altered oxygen supply’ in the case reports. · For signs and symptoms for ineffective airway clearance, ‘dyspnea’, ‘altered amount and character of sputum’ were included by 100% of the expert group. ‘Abnormal respiratory. sound(rate, rhonchi)’ were included by a high ratio of the expert group. · For the signs and symptoms for ineffective breathing pattern. ‘dyspnea’, ‘shortness of breath’ were included by 100% of the expert group. In the case reports, ‘dyspnea’ and ‘tachypnea’ were reported as signs and symptoms. · For the sign and symptoms for impaired gas exchange, ‘hypoxia’ and ‘cyanosis’ had a high ratio in the expert group. In the case report, ‘hypercapnia’, ‘hypoxia’ and ‘inability to remove secretions’ were reported as signs and symptoms. In summary, the similarity of the etiologies and signs and symptoms of the three nursing diagnoses related to difficulty in respiratory function makes it difficult to distinguish among them But the clinical validity of three nursing diagnoses was established through this study, and at last one sign and symp-tom was defined for each diagnosis.

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부분골밀도 측정을 통해 산출되는 체지방률의 임상적 유용성에 대한 평가 (The Clinical Usefulness Measurement of the Whole Body Percent Fat Calculated by the Part Bone Mineral Density Measurement)

  • 강영은;김은혜;김호성;최종숙;최우준
    • 핵의학기술
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    • 제15권1호
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    • pp.3-9
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    • 2011
  • 이중에너지 방사선 흡수계측법(Dual-energy X-ray Apsorptiometry, DXA)은 일반적으로 골밀도를 통한 골다공증 평가 및 치료목적으로 이용되어 왔으며 최근 비만에 대한 관심이 높아지면서 체지방검사 처방이 증가하고 있는 추세이다. 기존의 체지방 측정은 전신을 검사해야만 평가가 가능했지만 근래 DXA장비의 소프트웨어가 향상 되면서 요추와 대퇴부의 측정만으로도 전신체지방률을 추측 할 수 있게 되었다. 전신측정이 아닌 부분측정 값이 다른 검사방법으로 산출된 값과 비교하여 타당성을 갖는지 생체전기저항측정법(Bioelectrical Impedence Analysis, BIA)과 체질량 지수(Body Mass Index, BMI)와의 상관도 분석을 통해 알아보고자 한다. 2010년 3월부터 8월까지 본원 건강검진센터에 내원한 수검자 중 40세 이상의 여성 90명을 대상으로 DXA와 BIA를 통해 체지방률을 측정하였다. BMI는 신체 계측기를 통해 측정된 신장과 체중을 수검자 정보에 기입하여 자동으로 산출되는 값을 활용하였다. 또한 체중에 따른 차이가 있는지 알아보기 위해 BMI를 기준으로 저체중, 정상, 비만의 세 그룹으로 분류하여 각 그룹에서 BMI와 BIA, DXA 간의 상관도를 분석하였다. 저체중과 정상, 비만으로 나눈 세 그룹에서의 DXA와 BIA의 비교는 모든 그룹에서 통계적으로 유의한 차이를 보이지 않았다. 그룹으로 나누지 않은 상태에서의 상관분석결과 DXA로 측정된 체지방률은 BMI와 비교해 통계적으로 유의한 높은 상관관계를 나타냈으며(r=0.908, p<0.01) BIA와의 비교에서도 통계적으로 유의한 높은 상관관계를 보였다(r=0.927, p<0.01). 부분 골밀도 측정으로부터 추정된 전신체지방률이 BIA 그리고 BMI와 비교해 뛰어난 상관관계를 보여 정보의 신뢰도가 높다는 것을 확인하였다. 아직 추정된 전신체지방률에 관한 명확한 기준이 없어 골밀도측정만으로 체지방평가를 하기는 어렵다. 그러나 골밀도검사를 통해 골다공증의 진단은 물론 체지방에 관한 정보까지 얻을 수 있다는 점에서 매우 효율적이며 추후 전신골밀도검사로 측정된 체지방률과의 비교연구를 통해 더 객관적이고 임상적으로 유용한 정보 제공이 가능할 것으로 판단된다.

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시설 오이의 관비재배를 위한 토양용액과 엽병즙액중 질산태 농도 기준 설정 (A Criteria on Nitrate Concentration in Soil Solution and Leaf Petiole Juice for Fertigation of Cucumber (Cucumis sativus L.) under Greenhouse Cultivation)

  • 임재현;이인복;김홍림
    • 한국토양비료학회지
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    • 제34권5호
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    • pp.316-325
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    • 2001
  • 비료의 효율적 이용 및 과다 시비에 따른 환경오염을 경감하고자 토양 및 식물체 중 질산이온 분석을 통한 간이진단기술을 검토하였다. 농가현장에서 토양중 질산이온을 추출하기 위한 간이검정방법으로서 porous cup을 이용한 토양용액채취법과 생토용적추출법은 실험실 분석법인 2M KCl 침출법과 비교하였고, 질산이온 간이측정기구인 compact ion meter, nitrate ion meter, test strip-소형 reflectometer법 등에 의한 질산이온 측정값은 실험실 정밀 측정장비인 IC 측정값과 비교하였다. 토양내 질소수준이 다른 하우스환경 하에서 오이의 재배기간동안 토양용액채취법과 생토용적침출법에 의한 토양중 질산이온농도는 주기적으로 monitoring 하였고, 엽병중 질산이온농도는 엽병 위치별로 분석하였으며, 토양과 식물체중 질산이온 농도들은 오이의 최종 수량과 관련하여 2차 회귀식으로 plotting 하였다. 질산이온 간이측정장비로서는 test strip을 이용한 소형 reflectometer법이 실험실 정밀 분석법인 IC에 의한 측정값과 가장 밀접한 상관성을 보였다. Porous cup을 이용한 토양용액채취법은 관수후 경과시간, 토양깊이, 점적호스로부터의 거리 등에 따라 토양용액중 질산이온 농도의 공간적 변이가 컸으나, 생토용적침출법과 2M KCl 침출법간 질산함량은 높은 상관성이 인정되었다. 엽병즙액중 질산농도의 엽의 위치에 따라 질산농도의 변화가 심하였고, 토양내 질소 수준에 따라 상위엽과 하위엽간 질소 농도의 분포 또한 상이하였다. 토양 및 식물체 엽병중 질산함량과 오이수량간 2차 회귀식으로부터 최고수량과 일치하는 각각의 질산 함량 수준을 검토한 결과, 토양용액채취법은 $400mg\;l^{-1}$ 수준, 생토용적 침출법은 $300mg\;l^{-1}$ 수준, 엽병즙액의 경우는 $1400mg\;l^{-1}$ 수준이었다. 여기서 엽병즙액과 오이수량간 상관성은 pot 시험을 통해 비교하였고, pot 실험결과 $1500mg\;l^{-1}$ 수준의 엽병내 질산농도 수준에서 최고 수량을 보여 하우스 실험결과와 유사하였다.

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LC/TOFMS를 이용한 생체시료의 내인성 대사체 분석법 개발 (Method Development for the Profiling Analysis of Endogenous Metabolites by Accurate-Mass Quadrupole Time-of-Flight(Q-TOF) LC/MS)

  • 이인선;김진호;조수열;심선보;박혜진;이진희;이지현;황인선;김성일;이정희;조수연;최돈웅;조양하
    • 한국식품위생안전성학회지
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    • 제25권4호
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    • pp.388-394
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    • 2010
  • Metabolomics aims at the comprehensive, qualitative and quantitative analysis of wide arrays of endogenous metabolites in biological samples. It has shown particular promise in the area of toxicology and drug development, functional genomics, system biology and clinical diagnosis. In this study, analytical technique of MS instrument with high resolution mass measurement, such as time-of-flight (TOF) was validated for the purpose of investigation of amino acids, sugars and fatty acids. Rat urine and serum samples were extracted by selected each solvent (50% acetonitrile, 100% acetonitrile, acetone, methanol, water, ether) extraction method. We determined the optimized liquid chromatography/time-of-flight mass spectrometry (LC/TOFMS) system and selected appropriated columns, mobile phases, fragment energy and collision energy, which could search 17 metabolites. The spectral data collected from LC/TOFMS were tested by ANOVA. Obtained with the use of LC/TOFMS technique, our results indicated that (1) MS and MS/MS parameters were optimized and most abundant product ion of each metabolite were selected to be monitorized; (2) with design of experiment analysis, methanol yielded the optimal extraction efficiency. Therefore, the results of this study are expected to be useful in the endogenous metabolite fields according to validated SOP for endogenous amino acids, sugars and fatty acids.

한국인의 연조직측모 선호경향에 대한 연구 (A Study of Esthetic Facial Profile Preference In Korean)

  • 최준규;이기수
    • 대한치과교정학회지
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    • 제32권5호
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    • pp.327-342
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    • 2002
  • 연조직 측모는 교정치료와 진단에 있어서 중요한 부분이며, 안모 심미성에 대한 개념은 긴 역사를 가지고 변천하여 왔으며 지역이나 문화, 성별, 인종, 관습 등에 따라서 그 심미관이 상이하다. 본 연구에서는 대중들의 연조직측모 선호도를 조사하기 위하여 교정환자들의 주 연령층인 사춘기성장가속기 전의 초등학생, 사춘기 연령의 학생, 20대의 대학생들과 보호자의 주연령층인 40-50대 층을 대상으로 설문조사를 시행하였다. 설문지의 제작을 위해 기존의 심미적인 안면의 경, 연조직 형태에 관한 연구에서 얻은 연조직 평균계측치에 가까운 연조직 측모를 가진 20대 여성 한 명을 선정한 후 측모천연색 사진을 촬영하여 표준측모로 사용하였다 전체 설문지는 7 set로 이루어져 있으며, 각 set는 측모에 영향을 줄 수 있는 입술의 돌출도, 코, 이부의 높이와 Sn point를 표준측모에서 변화시킨 천연색 측모형들을 포함하고 있다. 가장 좋다고 생각되는 안모는 1점, 가장 좋지 않다고 생각되는 안모를 5점의 순서로 통계처리를 하여 각 응답자군들의 연조직측모 선호도를 알아보았으며, 성별, 연령에 따른 선호도의 차이를 조사하였다. 또한 대중들이 선호하는 안모와 선호하지 않는 안모와 하안면 측모의 심미적 평가를 위해 기존에 사용되는 기준선들간의 신뢰성을 평가하여 다음의 결과를 얻을 수 있었다. 1.응답자의 신뢰도를 평가시 유년기에서 다른 group들에 비해 낮은 값을 나타내었다. 이는 유년기에서는 아직은 측모선호도에 대한 관심이 없는 것으로 평가하였다. 2. 측모 선호도의 연령, 성별 차이는 뚜렷하지 않은 것으로 나타났다. 3. 가장 선호하는 측모보다 가장 선호하지 않는 측모에 대한 선택의 일치도가 더 높았다. 4.응답자의 group별로 순위의 일치성을 나타내는 Kendall의 일치계수(W)는 20대에서 가장 높게 나타났다 이는 다른 연령에 비해 측모에 대한 선호성에 대한 기준이 명확한 것을 나타낸다. 5. 응답자들이 가장 선호하는 측모의 구순돌출도는 심미적인 안모에 대한 이전 연구에서의 Ricketts의 nose tip-Pog line(E-line), Burstone의 Sn-Pog line(B-line)에 대한 평균치에 가까운 값을 나타내었다 이는 이들 기준선들이 임상적으로 유용하게 사용할 수 있음을 의미한다. 6. 응답자들이 가장 선호하지 않는 측모는 E-line과 B-line에 대해 구순이 너무 돌출되거나 후퇴된 경우였다. 7. 응답자들의 측모 선호도는 straight, concave, convex profile순으로 나타났다.