• Journal of Ginseng Research
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• 제46권2호
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• pp.266-274
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• 2022

Colon cancer, the third most frequent occurred cancer, has high mortality and extremely poor prognosis. Ginsenoside, the active components of traditional Chinese herbal medicine Panax ginseng, exerts antitumor effect in various cancers, including colon cancer. However, the detailed molecular mechanism of Ginsenoside in the tumor suppression have not been fully elucidated. Here, we chose the representative ginsenoside Rg3 and reported for the first time that Rg3 induces mitophagy in human colon cancer cells, which is responsible for its anticancer effect. Rg3 treatment leads to mitochondria damage and the formation of mitophagosome; when autophagy is inhibited, the clearance of damaged mitochondria can be reversed. Next, our results showed that Rg3 treatment activates the PINK1-Parkin signaling pathway and recruits Parkin and ubiquitin proteins to mitochondria to induce mitophagy. GO analysis of Parkin targets showed that Parkin interacts with a large number of mitochondrial proteins and regulates the molecular function of mitochondria. The cellular energy metabolism enzyme GAPDH is validated as a novel substrate of Parkin, which is ubiquitinated by Parkin. Moreover, GAPDH participates in the Rg3-induced mitophagy and regulates the translocation of Parkin to mitochondria. Functionally, Rg3 exerts the inhibitory effect through regulating the nonglycolytic activity of GAPDH, which could be associated with the cellular oxidative stress. Thus, our results revealed GAPDH ubiquitination by Parkin as a crucial mechanism for mitophagy induction that contributes to the tumor-suppressive function of ginsenoside, which could be a novel treatment strategy for colon cancer.

• 성인간호학회지
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• 제15권2호
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• pp.215-225
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• 2003

Purpose: Sasang constitutional medicine is Korea's creative medical science, that has attracted public attention. But for lack of indices in objectifying constitutions, there are difficulties for most of health professionals in approaching Sasang medicine. The purpose of this study was to compare the results of clinical diagnostic data according to the type of Sasang constitution. Method: The subjects were 195 examinees who had a general health check-up at A hospital. The instrument used for this study were Questionnaire of Sasang Constitution Class II(QSCCII) program for personal computer developed by Kim Sun Ho et al. Result: The distribution of constitutions of the subjects were the So-eum 46.7%, the Tae-eum 36.9% and the So-yang 16.4%. The level of body compositionrelated indices(body weight, obesity) of the Tae-eum were significantly higher than those of other constitutions. In heart-related indices, systolic BP were the highest in the Tae-eum. In liver-related indices, the level of triglyceride, SGPT were significantly higher in the Tae-eum. But the hematologic indices and lung-related indices were not showed significantly difference among any constitutions. Conclusion: The results of this study showed that there were significant difference of clinical diagnostic data according to Sasang constitutions, especially in the Tae-eum. So, to understand the human nature of nursing clients, nurses need to apply the oriental based medical theory, like Sasang constitution, to the discipline of nursing.

• 성인간호학회지
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• 제19권3호
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• pp.447-458
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• 2007

Purpose: This exploratory study was to identify the use of complementary and alternative medicine(CAM) in women with breast cancer and to analyze which factor influence CAM use. Methods: 114 patients with breast cancer were identified and approached. The instrument used to gather data were the common types, main reasons and effects as well as stress, mood states and fatigue. Results: 42.7% of all participants reported the use of CAM. The most common types of CAM used were Phellinus linteus, elm tree, and vitamin C. The main reasons for CAM use were to boost the immune system and to prevent recurrence. Prayer, massage, Shitake mushroom, and Ganoderma lucidum were, identified as being more effective than others. 93.6% of the respondents informed their physicians of their use of CAM. Logistic regression analysis determined that stress, mood states, and duration after diagnosis were factors significantly associated with CAM use. Conclusion: CAM use is increasing among patients with breast cancer. Health care professionals are in the main position to identify what treatments patients are using and implement CAM therapies that can be helpful to relieve patient symptoms related to treatment and psychological distress.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제1권1호
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• pp.148-160
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• 1990

본 논문은 아동분리반응척도 개발에 관한 것으로 그 신뢰도와 타당성, 그리고 정신과 입원 아동들에 대한 임상 연구 이용도를 검증하였다. 아동분리반응척도는 신뢰성과 타당성있는 연구 기구임이 나타났고 아동의 분리반응을 들의 정신병리와 구별지어주는데 유용한 것으로 나타났다. 이것은 또한 감별진단하는데 임상가들에게 도움이 될것으로 보이며 아동의 정신과 입원시 일어날수 있는 분리반응과 그 부작용에 대한 이해의 필요성을 시사하였다. 이 기초연구는 입원치료 프로그램은 입원 아동들의 발달적 요구와 능력 그리고 정신병리시 나타나는 특정한 결함에 대해 반드시 관심을 보여야 한다는 개념을 지지하였다.

• 대한의용생체공학회:의공학회지
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• 제35권1호
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• pp.1-7
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• 2014

Among semi-quantitative or fully quantitative lateral flow assay readers, an image sensor-based instrument has been widely used because of its simple setup, cheap sensor price, and compact equipment size. For all previous approaches, monochrome CCD or CMOS cameras were used for lateral flow assay imaging in which the overall intensities of all colors were taken into consideration to estimate the analyte content, although the analyte related color information is only limited to a narrow wavelength range. In the present work, we introduced a color CCD camera as a sensor and a color decomposition method to improve the sensitivity of the quantitative biosensor system which utilizes the lateral flow assay successfully. The proposed setup and image processing method were applied to achieve the quantification of imitatively dispensed particles on the surface of a porous membrane first, and the measurement result was then compared with that using a monochrome CCD. The compensation method was proposed in different illumination conditions. Eventually, the color decomposition method was introduced to the commercially available lateral flow immunochromatographic assay for the diagnosis of myocardial infarction. The measurement sensitivity utilizing the color image sensor is significantly improved since the slopes of the linear curve fit are enhanced from 0.0026 to 0.0040 and from 0.0802 to 0.1141 for myoglobin and creatine kinase (CK)-MB detection, respectively.

• Journal of Applied Biological Chemistry
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• 제49권4호
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• pp.143-147
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• 2006

A rapid PCR-based assay for detecting hepatitis B viral DNA(HBV DNA) in serum and plasma was developed using a new PCR instrument named GenSpector(TMC-1000, Samsung electronics). PCR was carried out using a chip-based platform, which enabled 50 PCR cycles with internal controls, and melting-curve analysis in 30 minutes. Verification of the amplified HBV DNA product and the internal control was based on specific melting temperatures(Tm) analysis, executed by the GenSpector software. Primers were designed within the region conserved through HBV genotypes A to F. The lower limit of detection was 840 copies/ml serum, conducted with serial dilutions of a HBV DNA positive control(ACCURUN 325 series 700, Boston Biomedica Inc.). The assay was also compared to another assay for HBV DNA(Versant HBV DNA 3.0 assay, Bayer HealthCare) for 200 samples(each 100 clinical negative and positive samples). The sensitivity and specificity were 100% matched. This rapid PCR-based assay is specific, reproducible, and enables qualitative detection of HBV DNA.

• 한국콘텐츠학회논문지
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• 제11권1호
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• pp.277-283
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• 2011

본 연구는 만 19세 이상의 성인 여성 374명을 대상으로 비용이 저렴하고 안전한 초음파 검사를 이용하여 자궁근종을 확인하였으며, 자궁근종이 진단된 모집단과 자궁근종이 없는 대조군을 대상으로 한국어판 스트레스 척도인 BEPSI-K(Brief Encounter Psychosocial Instrument, BEPSI-K) 설문서를 이용하여 스트레스와 자궁근종과의 상관관계를 알아보았다. 초음파 검사로 진단된 자궁근종의 유병률은 29.7%로 나타났으며, BEPSI-K 설문에 따른 스트레스와 자궁근종과의 상관관계를 교차분석한 결과, 자궁근종이 진단된 모집단이 대조군에 비하여 많은 스트레스를 받는 것으로 나타났다. 통계적 유의수준은 p<0.05로 하였다. 이는 명확한 원인이 밝혀지지 않은 현대 여성의 자궁근종 발생 요인 중 스트레스가 자궁근종의 발병 원인 중의 하나 임을 확인하였다.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 1999년도 하계종합학술대회 논문집
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• pp.551-554
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• 1999

In this paper, we developed a radial pulse transducer that has strain-gauge cantilever type load cell for total pulse detection on chongu arterial. The transducer consist of load cell and driving electronic circuits. Load cell consist of cantilever and two metal film strain gauge. The Pressure signal from chongu artery is delivered to load cell using artery rider that attached to cantilever Therefore the pressure signal convert to voltage signals by the developed transducer As the results of experiment, the developed transducer has very good linearity at pressure to voltage conversion. The total pulse detection transducer can detected three kinds of chongu artery pulse with conveniently.

• 여성건강간호학회지
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• 제10권4호
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• pp.283-290
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• 2004

Purpose: This study elicited Korean women's perceptions of the life situations that contribute to Hwabyung, a culture.bound psychiatric illness in Korea, and delineated a typology of the perceived causative factors for this illness. Method: A purposive sample of 21 Korean women was recruited from a church, a senior center in Seoul and a clinic that provides traditional Korean and westernized medical services to patients in the Gyeonggi area. Inclusion criteria were: 1) the diagnosis of Hwabyung by a traditional doctor or "stress reaction and depression" by a westernized doctor; and 2) a score greater than 40 on the Hwabyung Self.Report Instrument. Surveys using the HSRI and individual interviews using Qmethodology were used. Result: The average age of the participants was 53.7 years(range 35-84). The mean score on the HSRI was 46.2(range = 42-52). Data analysis showed that participants perceived three life situations to cause Hwabyung: vulnerable situation, lowered self.esteem, and negative life events. Conclusions: Korean women, suffering from Hwabyung, perceived at least three different causal patterns for this illness. Nursing care plans should be tailored to meet these differences.

• 동의생리병리학회지
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• 제25권4호
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• pp.708-714
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• 2011

This study was aimed to review the trend of case reports of neuropsychiatry disease. The case reports were collected by retrieving Journal of Oriental Neuropsychiatry and analyzing period were from 1990 to 2010. We analyzed them for publish year, research institution, patients, sorts of diseases, pattern identification diagnosis, instrument, treatment period, sorts of treatments, and treatment with western medicine. We analyzed 142 case reports and there were many kinds of neuropsychiatry diseases and instruments that were evaluated the process. The reserchers used various treatment with oriental medicine. However, the number of case reports have decreased since the year of 2008. Most of the research institutions were university and general oriental medical hospital and the portion of private hospitals was only 2.11%. For the development of practical clinical trial of oriental medicine, we needs more qualifying case report. We hope more reserchers for psychiatry disease of oriental medicine will be interested in the publication of case report and this will serve to produce advance of oriental medicine.