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Evaluation of the taxonomic rank of the terrestrial orchid Cephalanthera subaphylla based on allozymes

  • CHUNG, Mi Yoon;SON, Sungwon;CHUNG, Jae Min;LOPEZ-PUJOL, Jordi;YUKAWA, Tomohisa;CHUNG, Myong Gi
    • Korean Journal of Plant Taxonomy
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    • v.49 no.2
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    • pp.118-126
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    • 2019
  • The taxonomic rank of the tiny-leaved terrestrial orchid Cephalanthera subaphylla Miyabe & $Kud{\hat{o}}$ has been somewhat controversial, as it has been treated as a species or as an infraspecific taxon, under C. erecta (Thunb.) Blume [C. erecta var. subaphylla (Miyabe & $Kud{\hat{o}}$) Ohwi and C. erecta f. subaphylla (Miyabe & $Kud{\hat{o}}$) M. Hiro]. Allozyme markers, traditionally employed for delimiting species boundaries, are used here to gain information for determining the taxonomic status of C. subaphylla. To do this, we sampled three populations of five taxa (a total of 15 populations) of Cephalanthera native to the Korean Peninsula [C. erecta, C. falcata (Thunb.) Blume, C. longibracteata Blume, C. longifolia (L.) Fritsch, and C. subaphylla]. Among 20 putative loci resolved, three were monomorphic (Dia-2, Pgi-1, and Tpi-1) across the five species. Apart from C. longibracteata, there was no allozyme variation within the remaining four species. Of the 51 alleles harbored by these 17 polymorphic loci, each of the 27 alleles at 14 loci was unique to a single species. Accordingly, we found low average values of Nei's genetic identities (I) between ten species pairs (from I = 0.250 for C. erecta versus C. longifolia to I = 0.603 for C. falcata vs. C. longibracteata), with C. subaphylla being genetically clearly differentiated from the other species (from I = 0.349 for C. subaphylla vs. C. longifolia to 0.400 for C. subaphylla vs. C. falcata). These results clearly indicate that C. subaphylla is not genetically related to any of the other taxa of Cephalanthera that are native to the Korean Peninsula, including C. erecta. In a principal coordinate analysis (PCoA), C. subaphylla was positioned distant not only from C. falcata, C. longibracteata, and C. longifolia, but also from C. erecta. Finally, K = 5 was the best clustering scheme using a Bayesian approach, with five clusters precisely corresponding to the five taxa. Thus, our allozyme results strongly suggest that C. subaphylla merits the rank of species.

Centrifuge Model Test on the Bearing Capacity and Failure Mechanism of Composit Ground Improved with Slag Compaction Piles (슬래그 다짐말뚝으로 개량된 복합지반의 지지력 및 파괴메카니즘에 관한 원심모형실험)

  • Yoo Nam-Jae;Park Byung-Soo;Jeong Gil-Soo;Koh Kyung-Hwan;Kim Ji-Sung
    • Journal of the Korean Geotechnical Society
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    • v.21 no.1
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    • pp.59-67
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    • 2005
  • This paper presents experimental and numerical research results of centrifuge model tests performed to investigate the geotechnical engineering behavior of slag compaction pile as a substitute of sand compaction pile. For centrifuge model tests, bearing capacity of composit soil improved with slag compaction piles, stress concentrations in-between pile and soft clay, settlement characteristics, and failure modes were investigated with slags differing in their relative density. A slag was found to be a good substitute for a sand since the slag compaction pile model showed a greater yield stress intensity up to $30\%$ than the sand compaction pile model under the identical testing conditions. Stress concentration ratio tended to increase with the relative density of slag pile and the clear shear lines in the piles were observed at the depth of $2D{\sim}2.5D$ (D=dia. of model pile) from the top of the piles after loading tests. Numerical analysis with a software of CRISP, implemented with the modified Cam-clay model, was carried out to simulate the results of centrifuge model test. Test results about characteristics of load-settlement curves and stress concentration ratio are in relatively good agreements with numerical estimations.

Projective Reconstruction Method for 3D modeling from Un-calibrated Image Sequence (비교정 영상 시퀀스로부터 3차원 모델링을 위한 프로젝티브 재구성 방법)

  • Hong Hyun-Ki;Jung Yoon-Yong;Hwang Yong-Ho
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.42 no.2 s.302
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    • pp.113-120
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    • 2005
  • 3D reconstruction of a scene structure from un-calibrated image sequences has been long one of the central problems in computer vision. For 3D reconstruction in Euclidean space, projective reconstruction, which is classified into the merging method and the factorization, is needed as a preceding step. By calculating all camera projection matrices and structures at the same time, the factorization method suffers less from dia and error accumulation than the merging. However, the factorization is hard to analyze precisely long sequences because it is based on the assumption that all correspondences must remain in all views from the first frame to the last. This paper presents a new projective reconstruction method for recovery of 3D structure over long sequences. We break a full sequence into sub-sequences based on a quantitative measure considering the number of matching points between frames, the homography error, and the distribution of matching points on the frame. All of the projective reconstructions of sub-sequences are registered into the same coordinate frame for a complete description of the scene. no experimental results showed that the proposed method can recover more precise 3D structure than the merging method.

Effect of Si contents on Tensile-Shear Peak Load and Nugget Diameter in the Resistance Spot Welded of Dual Phase Steel for Automotive Body Applications (자동차 차체용 냉연 DP강 저항점용접부의 너깃경과 인장전단강도에 미치는 Si 함유량의 영향)

  • Kong, Jong-Pan;Park, Tae-Jun;Han, Tae-Kyo;Chin, Kwang-Geun;Kang, Chung-Yun
    • Proceedings of the KWS Conference
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    • 2009.11a
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    • pp.45-45
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    • 2009
  • 원가 측면에서 유리한 저항점용접(Resistance Spot Welding)이 차체 용접에 80%이상으로 가장 많이 적용되고 있다. 첨단고강도강(Advanced High Strength Steel)의 저항점용접성 및 용접부 특성에 미치는 공정 변수의 영향에 대한 연구결과는 많으나, 합금원소의 영향에 대해서는 전무하다. 특히, Si는 DP(Dual Phase)강에 첨가 시 균일한 마르텐사이트의 분포를 촉진하는 원소로 저항 점용접성 및 용접부 특성에 영향을 미칠 것으로 예상되며, 이에 대한 연구는 보고된바 없다. 본 연구에서는 냉연 DP강의 저항 점용접시 중요한 인자 중 하나인 너깃경과 전단인장강도에 미치는 Si함유량의 영향을 검토하였다. 사용된 강재 및 용접기는 1.2mm 두께의 Si함유량(0, 0.5, 1.0, 1.5wt%)이 다른 인장강도 780~1000MPa급 냉연 DP강과 단상 AC용접기를 사용하였다. 용접조건은 ISO 18278-2규격에 따라 가압력 4kA, 초기가압시간 40cycle, 유지시간 17cycle로 고정하고, 용접전류만 변화하여 용접을 실시하였다. 너깃경은 용접부 단면을 컷팅 후 폴리싱 하여, 광학현미경과 Image Pro plus를 이용하여 측정했으며, 인장시편규격은 JIS Z 3137를 이용하였다. Si함유량이 증가에 따라 스패터 발생 전류는 감소했고, 너깃경은 직선적으로 증가했다. Si함유량 증가에 따른 너깃경 증가 이유는 저항(R) 측정결과, Si함유량 증가에 따라 모재의 저항이 높아져, 따라서 입열량($Q=I^2Rt$)이 많아지기 때문으로 판단되었다. 인정전단강도는 Si함유량 증가에 따라 직선적으로 증가했다. 이러한 이유는 Si함유량 증가에 따라 너깃경이 증가되기 때문으로 판단되었고, 너깃경과 인장전단강도 사이에 직선적 관계(PL(kN)=$3.2N_{dia.}$-0.81, $R^2$=0.93)를 가지고 있었다. 파단양상은 Si함유량에 상관없이 5.4kA이하에서는 계면파단이 일어났고, 6.0kA이상에서는 풀 아웃 파단이 일어났다. 계면파단주원인은 용접부 가장자리에 지름이 약 $5{\mu}m$이하의 예리한 노치가 존재하여 노치응력집중과 HAZ계면 근처에 미접합부가 존재하기 때문으로 판단되었다. 6.0kA이상에서는 예리한 노치가 없었고, HAZ부가 완전히 접합되어 있기 때문에 풀 아웃 파단이 일어난 것으로 판단되었다. 따라서, Si함유량 증가에 따라 적정용접전류 구간은 감소했고, 너깃경은 직선적으로 증가했다. 또한, Si함유량 증가에 따라 인장전간강도는 증가 했으며, 너깃경과 인장전단강도 사이에 직선적 관계를 가지고 있었다. 파단 양상은 Si함유량에 상관없이 5.2kA이하에서는 계면파단이, 6.0kA이상에서는 풀 아웃 파단이 일어났다.

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On the Development of Toilets in Korean Rural Areas for Preventing Transmission of Communicable Diseases (질병전염 방지를 위한 농촌변소 개량에 관한 연구)

  • 정문식;정문호
    • Journal of Environmental Health Sciences
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    • v.6 no.1
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    • pp.1-9
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    • 1979
  • An experimental study was carried out to develop a rural type toilet of which the effluent could not transmit parasitic diseases at a village in Kangwon Province, Korea, during the period of January through December 1978, A drum tank (dia. 57cm$\times$90cm) and a cement tank (100cm$\times$100cm$\times$100cm) were filled with human excreta collected from toilets of the villages (the ratio of feces to urine was estimated approximately 1: 5) at once and three threecompartment toilets were constructed and used by people. pH, temperatures and viability of parasitic eggs were examined with the content of toilets. Rusults are summarized as follows: 1. pH increased from 7.0 at the beginning of experiment to 7.5 or 8.0 after 4 months of storage in drum tank as well as in cement tank and so did from 7.0~7.5 in the first tank to 8.0~8.5 in the third tank of all three-compartment toilets. 2. Temperatures of content at middle part of toilets in January through March ranged from 2 to 6$\circ$C which were 2-4$\circ$C higher than those of air, and those of lower part were again 1~2$\circ$C higher than of middle part. but temperatures of air, at middle part andat lower part in April were 14$\circ$C, 9~10$\circ$C and 8~9$\circ$C respectively, in July 29$\circ$C, 20~21$\circ$C and 19~20$\circ$C respe ctively and in October 17$\circ$C, 14$\circ$C and 14~13$\circ$C respectively. 3. All the parasitic eggs were degenerated about 4 months after filling drum tank with human excreta on 10th April while 10% of eggs were degenerated on 15th May, and all the eggs were degenerated about 4 months after filling cement tank on 24th August while about 10% were degenerated on 11th September and 20% on 4th October. 4. Degeneration rates of eggs were only 5~15% at 5cm below surface in the first tanks of three-compartment toilets while 45~65% at 50cm below, and concentration rates of eggs in second tanks were 8~12% of those in first tanks and only a few eggs were found in third tank but all of them were degenerated. Specific gravity of liquid of 1.022~1.024 in second tanks was not enough for overflowing eggs into third tanks.

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Effect of Core Morphology on the Decomposition of CCI₄ over the Surface of Core/Shell Structured Fe₂O₃/MgO Composite Metal Oxides

  • 김해진;강진;박동곤;권호진;Kenneth J. Klabunde
    • Bulletin of the Korean Chemical Society
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    • v.18 no.8
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    • pp.831-840
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    • 1997
  • Core/shell structured composite metal oxides of Fe2O3/MgO were prepared by thermal decomposition of Fe(acac)3 adsorbed on the surface of MgO cores. The morphology of the composites conformed to that of the MgO used as the cores. Broad powder X-ray diffraction peaks shifted toward larger d, large BET surface area (∼350 m2/g), and the size of crystalline domains in nano range (4 nm), all corroborate to the nanocrystallinity of the Fe2O3/MgO composite which was prepared by using nanocrystalline MgO as the core. By use of microcrystalline MgO as the core, microcrystalline Fe2O3/MgO composite was prepared, and it had small BET surface area of less than 35 m2/g. AFM measurements on nanocrystalline Fe2O3/MgO showed a collection of spherical aggregates (∼80 nm dia) with a very rough surface. On the contrary, microcrystalline Fe2O3/MgO was a collection of plate-like flat crystallites with a smooth surface. The nitrogen adsorption-desorption behavior indicated that microcrystalline Fe2O3/MgO was nonporous, whereas nanocrystalline Fe2O3/MgO was mesoporous. Bimodal distribution of the pore size became unimodal as the layer of Fe2O3 was applied to nanocrystalline MgO. The macropores in a wide distribution which the nanocrystalline MgO had were absent in the nanocrystalline Fe2O3/MgO. The decomposition of CCl4 was largily enhanced by the overlayer of Fe2O3 on nanocrystalline MgO making the reaction between nanocrystalline Fe2O3/MgO and CCl4 be nearly stoichiometric. The reaction products were environmentally benign MgCl2 and CO2. Such an enhancement was not attainable with the microcrystalline samples. Even for the nanocrystalline MgO, the enhancement was not attained, if not with the Fe2O3 layer. Without the layer of Fe2O3, it was observed that the nanocrystalline domain of the MgO transformed into microcrystalline one as the decomposition of CCl4 proceeded on its surface. It appeared that the layer of Fe2O3 on the particles of nanocrystalline Fe2O3/MgO blocked the transformation of the nanocrystalline domain into microcrystalline one. Therefore, in order to attain stoichiometric reaction between CCl4 and Fe2O3/MgO core/shell structured composite metal oxide, the morphology of the core MgO has to be nanocrystalline, and also the nanocrystalline domains has to be sustained until the core was exhausted into MgCl2.

Determination of the Optimal Nitrogen Concentration in Pre-planting Fertilizers for the Cultivation of Tomato Plug Seedlings

  • Lee, Dong Hoon;Park, Myong Sun;Lee, Chiwon W.;Choi, Jong Myung
    • Horticultural Science & Technology
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    • v.35 no.4
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    • pp.431-438
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    • 2017
  • This study investigated the effect of pre-planting nitrogen (N) fertilization levels added to a soilless root medium on the growth of 'Dotaerang Dia' tomato seedlings. The N levels were varied for a total of 7 treatments: 0, 100, 250, 500, 750, 1,000, or $1,500mg{\cdot}L^{-1}$. The pH of the root media gradually rose in all treatments as the seedlings grew; however, the differences in the pH were not significant among the treatments. The electrical conductivity (EC) of the root media was significantly different among the treatments from sowing to week three, then drastically decreased after week four, which diminished the differences in the EC among the treatments. At week six, plant height, leaf length, leaf width, number of leaves, and fresh and dry weights of the shoot were highest for the treatment with $500mg{\cdot}L^{-1}N$. In contrast, the treatment with $1,500mg{\cdot}L^{-1}N$ had the lowest results for all growth measurements. The fresh weight was 67% heavier in the $500mg{\cdot}L^{-1}N$ treatment compared to the $1,500mg{\cdot}L^{-1}N$ treatment. The total N content in the tissues was lowest in the treatment with $0mg{\cdot}L^{-1}N$ and highest in the treatment with $1,000mg{\cdot}L^{-1}N$. The contents of calcium (Ca), magnesium (Mg), and metal micronutrients in the tissues were highest in the $250mg{\cdot}L^{-1}N$ treatment. A previous study demonstrated that adjusting the fertilization level to promote growth to over 90% of the maximum growth is a good strategy for lowering production costs and preventing damage due to excessive fertilizer absorption by crops. Our results indicated that the optimal pre-planting N fertilization level for tomato plug seedlings should be lower than $500mg{\cdot}L^{-1}$ and the optimum tissue N contents should be around 3.21% to 4.60%.

Characteristic of Buckling and Ultimate Strength of the Perforated Stiffened Plate (유공보강판의 좌굴 및 극한강도 특성에 관한 연구)

  • Park Joo-Shin;Ko Jae-Yong;Oh Kyoung-Gun
    • Journal of Navigation and Port Research
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    • v.30 no.6 s.112
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    • pp.439-446
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    • 2006
  • In ship structures many of the structural plates have cutouts, for example, at inner bottom structure, girder, upper deck hatch, floor and dia-frame etc. In the case where a plate has a cutout it experiences reduced buckling and ultimate strength and at the same time the in-plane stress under compressive load produced by hull girder bending will be redistributed In general, actual ship structure adopted reinforcement of stiffener around the cutout in order to preventing from buckling so it need to examine a buckling and ultimate strength behaviour considering a cutout because In many ship yards used class rule for calculating buckling strength but it is difficult to evaluate perforate stiffened plate with random size. In the present paper, we investigated several kinds of perforated stiffened model from actual ship and then was performed finite element series analysis varying the cutout ratio, web height, thickness and type of cross-section using commercial FEA program(ANSYS) under compressive load.

Fabrication and Magnetic Properties of BaFe12-2xCoxTixO19 Powders (BaFe12-2xCoxTixO19 분말의 제조 및 자기특성 연구)

  • An, Sung-Yong;Shim, In-Bo;Kim, Chul-Sung;Lee, Seung-Wha
    • Journal of the Korean Magnetics Society
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    • v.13 no.1
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    • pp.21-28
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    • 2003
  • M-type hexagonal BaF $e_{12-2x}$ $Co_{x}$ $Ti_{x}$ $O_{19}$ (0$\leq$x$\leq$1.0) ferrite powders prepared by a sol-gel method. The crystallographic and magnetic properties were characterized with a x-ray diffraction (XRD), thermogravimetry (TG), differential thermal analysis (DIA), Fourier transform infrared spectroscopy (FT-IR), vibrating sample magnetometer (VSM), and Mossbauer spectroscopy. The result of XRD measurements show that the a and c lattice parameters increase with increasing x from $\alpha$=5.882 and c=23.215 $\AA$ for $\chi$=0.0, to $\alpha$=5.895 and c=23.295 $\AA$ for $\chi$=1.0. From the Mossbauer results, the $Co^{2+}$- $Ti^{4+}$ site occupancies have been affected the changes in the magnetization and in the coercivity. The Curie temperature linearly decreases with increasing $Co^{2+}$- $Ti^{4+}$ concentration x.

Development of Recycling Technology for Used Cables (폐전선 재활용 기술개발)

  • 양정일;오정완;최우진;황선국
    • Resources Recycling
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    • v.3 no.2
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    • pp.28-34
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    • 1994
  • A part of used cables, such as electric and communication cables has already been recycled by using simple processing methods. However, it has been found that the main problems in recycling of the used cables are insufficient treatment of fine stranded wires and low recovery of copper by air separation process. It has been shown that copper can be effectively separated from the PE using a solvent treatment method. In the present study, the used communication wires having diameter of 0.4 mm are treated in the mixing solution of toluene and water at $86^{\circ}C$ for about 10 minutes. In the solvent treatment, the copper wires recovered have 10~15mm length, which are much longer than that of 1~2mm length copper wires recovered by air table concentration method used in current recycling plants. The process consisting of cutting, air separation and electrostatic separation would be recommendable for the treatment of mixed cables. In this investigation, fine copper powders can also efficiently be recovered from insulation materials using electrostatic separator at the conditions of 20~50RPM roller speed and 15~30KV high DC power.

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