• Title/Summary/Keyword: DiaA

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Immunoelectron microscopic localization of partially purified antigens in adult Paragonimus iloktsuenensis

  • Lee, Ok-Ran;Chung, Pyung-Rim
    • Parasites, Hosts and Diseases
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    • v.39 no.2
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    • pp.119-132
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    • 2001
  • An immunoelectron microscopy employing immunogold labeling method was performed to detect tissue origin of Dl fraction (DIA) among 5 antigenic protein fractions partially purified by DEAE- anion exchange chromatography from water- soluble crude antigen (PIWA) of adult Paragonimus iloktsuenensis. Immune reactions of adult worm tissues with rabbit serum immunoglobulin immunized with crude antigen (PI-Ig) and D1 antigen (D1-Ig), as well as rat serum immunoglobulin infected with P. iloktsuenensis were observed. DlA showed strong antigenicity in the intestinal epithelium of the worms during the early infection period of 2-4 weeks after infection. The vitellaria also showed stronger antigenicity than the other tissue sites in immune reaction of tissues against all immunoglobulins from 4 to 33 weeks after vitelline development. Therefore, it is suggested that DlA was mainly originated from the intestinal epithelial tissues before the development of vitelline gland of the parasites. Immune-reactivity of two immunoglobulins (PI-Ig, Dl-Ig) was significantly different in intestinal epithelial cytoplasmic protrusions (CP) and intestinal epithelial secretory granules (SG). In the experimental group with Dl-Ig, gold particles were labeled significantly in CP than in SG when compared to the PI-Ig group. Thus, the major antigenic materials in Dl antigen having a strong antigenicity in the early infection period was considered to be originated from the intestinal epithelial tissue .

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A New Image Compression Technique for Multimedia Teleconferences (멀티미디어 텔레컨퍼런스를 위한 새로운 영상 압축 기술)

  • Kim, Yong-Ho;Chang, Jong-Hwan
    • The Journal of Natural Sciences
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    • v.5 no.2
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    • pp.33-38
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    • 1992
  • A new texture segmentation-based image coding technique which performs segmentation based on roughness of textural regions and properties of the human visual system (HVS) is presented for multime-dia teleconference. The segmentation is accomplished by thresholding the fractal dimension so that textural regions are classified into three texture classes; perceived constant intensity, smooth texture, and rough texture. An image coding system with high compression and good image quality is achieved by developing an efficient coding technique for each segment boundary and each texture class. We compare the coding efficiency of this technique with that of a well established technique (discrete cosine transform (DCT) image coding).

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Influence of Artificial Defect on Fatigue Limit in Austempered Ductile Iron (오스템퍼링처리한 구상흑연주철의 피로한도에 미치는 인공결함의 영향)

  • Kim, Min-Geon;Kim, Jin-Hak
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.23 no.11 s.170
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    • pp.1922-1928
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    • 1999
  • Rotary bending fatigue tests were carried out to investigate the influence of artificial defects on fatigue limit in annealed and austempered ductile iron. Obtained main results are as follows : (1) Artificial defect(micro hole type, dia.<0.4 mm) on specimen surface did not bring about a obvious reduction of fatigue limit in austempered ductile iron(ADI) as compared with annealed ductile iron. (2) According to the investigation of $\sqrt{area}_c$ which is the critical defect size to crack initiation at artificial defect, $\sqrt{area}_c$ of ADI is larger than that of annealed ductile iron. This shows that the situation of crack initiation at artificial defect in ADI is more difficult in comparison with annealed ductile iron. (3) One of the reasons for the low rate of crack initiation from artificial defect in ADI is that the resistance of matrix to crack initiation is higher than that of annealed ductile iron. (4) In case that the $\sqrt{area}$ of artificial defect and graphite nodule is the same, the rate of crack initiation from graphite nodule is higher than that from artificial defect. This reason is that the serious ruggedness around graphite nodule is formed by austempering treatment.

The Fabrication of Hydroxyapatite Targets and the Characteristics of Hydroxyapatite/Ti-6Al-4V Alloy Thin Films by RF Sputtering(I) (RF 스퍼터링용 Hydroxyapatite 타겟의 제조 및 Hydroxyapatite/Ti-6Al-4V 합금 박막의 특성(I))

  • Jung, Chan-Hoi;Kim, Myung-Han
    • Korean Journal of Materials Research
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    • v.13 no.4
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    • pp.205-212
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    • 2003
  • RF sputtering process was applied to produce thin hydroxyapatite[HA, Ca10($PO_4$)$_{6}$ $ (OH)_2$films on Ti-6Al-4V alloy substrates. To make a 101.6 mm dia.${\times}$5 mm HA target, the commercial HA powder was first calcinated for 3h at $200^{\circ}C$. A certain amount of the calcinated HA powder was pressed under a pressure of 20,000 psi by the cold isostatic press(CIP) and the pressed HA target was sintered for 6 h at $1,200^{\circ}C$. The effects of different heat treating conditions on the bonding strength between HA thin films and Ti-6Al-4V alloy substrates were studied. Before deposition, the alloy substrates were annealed for 1 h at $850^{\circ}C$ under $3.0${\times}$10^{-3}$ Xtorr, and after deposition, the hydroxyapatite/Ti-6Al-4V alloy thin films were annealed for 1 h at 400, 600 and $800^{\circ}C$ under the atmosphere, respectively. Experimental results represented that the HA thin films on the annealed substrates had higher hardness than non-heat treated substrates before the deposition.

Effective Extraction of Oligomeric Proanthocyanidin (OPC) from Wild Grape Seeds

  • Huh Yun Suk;Hong Tae Hee;Hong Won Hi
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.9 no.6
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    • pp.471-475
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    • 2004
  • The Oligomeric proanthocyanidin (OPC) in green and black tea, grape seeds, grapes and wine has raised much attention but that OPC in wild grape seed remains to be intensively investigated. This study investigated the total OPC contents and total antioxidant activity of wild grape seeds and developed an efficient extraction process with various temperatures, solvent compositions and times. Also, a chromatography column packed with the Dia-ion HP-20 resin was used for further purification of the ope. The total OPC contents were determined with the Folin-Ciocalteu reagent, and the antioxidant activity using total antioxidant potential (TAP) and 1,1-dipheny|-2picrylhydrazy| (DPPH). The yield of final purified OPC was 1.78 (+)-catechin equivalent (CE) g/100 g, with $IC_{50}$ activities of TAP and DPPH of 31.60 and $15.70\;{\mu}\;g/mL$. These activi­ties of the final purified OPC were about two times higher than that of the BHA used as a refer­ence sample.

Lactobacillus plantarum을 용균시키는 Bacteriophoge SC921의 분리 및 특성

  • Yoon, Sung-Sik;Shin, Young-Jae;Choi, Hak-Jong;Her, Song;Oh, Doo-Hwan
    • Microbiology and Biotechnology Letters
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    • v.25 no.1
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    • pp.96-101
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    • 1997
  • Among the lactic flora responsible for the development of acidity and characteristic flavor of Kimchi which is a traditional fermented Chiness cabbage. Homofermentative Lactobacillus plantarum is rod-shaped and to be known to ewert major role during later fermentation period. Once this strain establishes main flaora in the Kimchi fermentation process, it gives rise to excess acid production to reduce the taste and quality of Kimchi during storage. As a primary work to increase the keeping quality using virulent Lactobaillus plantarum bacteriophages, it was isolated sucessfully from collected Kimchi samples and their characteristics were studied. The new isolated phage, named SC 921, adsorbed to its host without Ca$^{2+}$, and nearly eliminated at 60$\circ $C of heat treatment for 5 min. This phages were atable at pH 4~ 10 but inactivated below pH 3.0 or pH 11.0 above. The latent period, rise period, and burst size of this phage was 100 min, 120 min, 31$\pm $2pfu/ml, respectively. Electron micrograph showed the phages particles were unusually oval feature of head (dia 80~ 120 nm) without contractile tail.

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Systems Engineer Program for Practical Nuclear Power Plant Engineering Education (실용적인 원전공학 교육을 위한 시스템즈 엔지니어 프로그램)

  • Chang, Choong-koo;Jung, Jae-cheon;DIA, Aminata
    • Journal of the Korean Society of Systems Engineering
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    • v.11 no.2
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    • pp.31-40
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    • 2015
  • KEPCO International Nuclear Graduate School (KINGS) is dedicated to nurturing leadership-level professionals in nuclear power plant (NPP) engineering. KINGS have designed curriculum based on two philosophies. First, we balance aspects of discipline engineering, specialty engineering, and management engineering in the framework of systems engineering. Second, KINGS have designed the curriculum so that students can learn and experience the know-what, know-how and know-why level knowledge of NPP engineering and management. The specialization programs are opened during the 2nd year for 3 trimesters and those are a process of learning through practical project courses. The objectives of the specialization programs are to help students to learn the NPP life cycle technologies in highly structured and systematic ways. The systems engineer program (SEP) is one of the specialization programs. A practical case of the SEP which was applied to the project course for the NPP electric power system design education will be elaborated in this paper.

Effects of Scintillation Crystal Surface Treatments on Gamma Camera Imaging (섬광체 옆표면처리가 감마카메라 영상에 미치는 효과)

  • Kim, J.H.;Choi, Y.;Oh, C.H.;Kim, J.Y.;Lee, M.Y.;Kim, S.E.;Choe, Y.S.;Joo, K.S.;Kim, B.T.
    • Proceedings of the KOSOMBE Conference
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    • v.1998 no.11
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    • pp.303-304
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    • 1998
  • We investigated the effects of scintillation crystal surface treatment on gamma camera imaging. The NaI(Tl) and CsI(Tl) (20 mm (dia.) $\times10mm$ (thick) plate) scintillators were chosen for this study. Two different surface treatments, white and black reflectors, were applied to NaI(Tl) and CsI(Tl). The optical properties of generated scintillation light were evaluated using Monte Carlo simulation and postion sensitive photo multiplier tube (PSPMT). We measured sensitivity, energy resolution and spatial resolution of a gamma camera system with the scintillators coupled to a PSPMT. Based on the results, we concluded that the careful consideration of surface treatments of the scintillator was necessary in order to develop the gamma camera having good sensitivity and spatial resolution.

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A Study on the Superplastic Characteristics of Sn-38%Pb Eutectic Alloy Produced by Continuous Casting Process (연속주조법에 의한 Sn-38%Pb 공정합금의 초소성특성)

  • Song, Tae-Seok;Jo, Hyung-Ho;Choi, Jae-Ha;Ji, Tae-Gu;Kim, Myung-Han
    • Journal of Korea Foundry Society
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    • v.17 no.3
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    • pp.252-257
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    • 1997
  • The 8 mm dia: Sn-38%Pb eutectic alloy rods were produced by use of the horizontal continuous casting process with the heated mold and chill cast process. The cast rods were rolled into 2.0${\sim}$0.5 mm thick plates and structural and mechanical properties were examined. The results revealed that the plates produced by the continuous casting process with the heated mold show much higher superplasticity at ambient temperature (1550% elongation at 0.5mm thick plate and 0.5mm/min strain rate) than the plates procuced by chill cast process (630% elongation). Such a high superplasticity of the continuous cast plates is due to the high-quality plates free from any inside and surface defects with fine and uniform distribution of eutectic phases.

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The Effect of Hull Appendages on Maneuverability of Naval Ship by Sensitivity Analysis (민감도 해석을 통한 선체 부가물이 함정의 조종성능에 미치는 영향 분석)

  • Kim, Dae Hyuk;Rhee, Key-Pyo;Kim, Nakwan
    • Journal of the Society of Naval Architects of Korea
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    • v.51 no.2
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    • pp.154-161
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    • 2014
  • Naval ships have hull appendages which are more exposed to the outside because of its small block coefficient compared with commercial ships. These exposed hull appendages like skeg, strut and shaft line affect the maneuverability of a ship. The effect of hull appendages has considered at initial design stage to estimate more accurate maneuverability. In this paper, sensitivity analysis is used to analyze the effect on maneuverability by hull appendages. 3 DOF maneuvering equations based on Mathematical Modelling Group (MMG) model are used and propeller & rudder model are modified to reflect the characteristics of twin propeller & twin rudder. Numerical maneuvering simulations (Turning test, Zig-zag test) for benchmark naval vessel, David Taylor Model Basin (DTMB) 5415 are performed. In every simulation, it is calculated that stability indices and maneuverability characteristics (Tactical Dia., Advance, 1st Overshoot, Time of complete cycle) with respect to the parameters (area times lift coefficient slope, attachment location) of hull appendages. As a result, two regression formulas are established. One is the relation of maneuverability characteristics and stability indices and the other is the relation of stability indices and hull appendages.