• Journal of Microbiology and Biotechnology
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• 제33권7호
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• pp.915-925
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• 2023

Sarcopenia is defined as loss of muscle mass and strength due to aging. Recent studies show that sarcopenia may improve via the gut-muscle axis, suggesting that gut health may affect muscle phenotypes. In this study, we aimed to investigate the ability of Lactobacillus rhamnosus JY02 as a probiotic strain isolated from kimchi to alleviate sarcopenia. L. rhamnosus JY02-conditioned medium (CM) reduced dexamethasone (DEX)-induced myotube diameter atrophy and expression of muscle degradation markers (MuRF1 and atrogin-1) in C2C12 cells. The amelioration of sarcopenia was investigated by measuring body composition (lean mass), hand grip strength, myofibril size (using histological analysis), and mRNA and protein expression of muscle-related factors in a DEX-induced mouse model. The results of these analyses showed that L. rhamnosus JY02 supplementation promoted the production of muscle-enhancement markers (MHC Iβ, MHC IIα, and Myo-D) and reduced both the production of muscle degradation markers and the symptoms of muscle atrophy (loss of lean mass and muscle strength). We also found decreased levels of pro-inflammatory cytokines (IL-6, IFN- γ) and increased levels of anti-inflammatory cytokines (IL-10) in the serum of DEX+JY02-administered mice compared to those in DEX-treated mice. Overall, these results suggest that L. rhamnosus JY02 is a potent probiotic supplement that prevents sarcopenia by suppressing muscle atrophy.

• IMMUNE NETWORK
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• 제22권4호
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• pp.33.1-33.17
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• 2022

Suppressors of cytokine signaling (SOCS) have emerged as potential regulators of macrophage function. We have investigated mechanisms of SOCS3 action on type 2 macrophage (M2) differentiation induced by glucocorticoid using human monocytic cell lines and mouse bone marrow-derived macrophages. Treatment of THP1 monocytic cells with dexamethasone (Dex) induced ROS generation and M2 polarization promoting IL-10 and TGF-β production, while suppressing IL-1β, TNF-α and IL-6 production. SOCS3 over-expression reduced, whereas SOCS3 ablation enhanced IL-10 and TGF-β induction with concomitant regulation of ROS. As a mediator of M2 differentiation, glucocorticoid-induced leucine zipper (GILZ) was down-regulated by SOCS3 and up-regulated by shSOCS3. The induction of GILZ and IL-10 by Dex was dependent on ROS and p38 MAPK activity. Importantly, GILZ ablation led to the inhibition of ROS generation and anti-inflammatory cytokine induction by Dex. Moreover, GILZ knock-down negated the up-regulation of IL-10 production induced by shSOCS3 transduction. Our data suggest that SOCS3 targets ROS- and p38-dependent GILZ expression to suppress Dex-induced M2 polarization.

• 천문학회지
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• 제29권2호
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• pp.181-194
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• 1996

We present a study of the metallicity of the old open cluster NGC 1245 , based on the Washington CCD photometry obtained using the 0.6 m telescope at the Sobaeksan Observatory, Korea. NGC 1245 has been known to be a unique cluster among the known open clusters in the sense that the previous metallicity estimates for this cluster are much larger $(by\;\sigma)$ than the value expected from the radial metallicity gradient of the old open clusters in Our galaxy. We have estimated the metallicity of the cluster red giants using the four color-color diagrams, obtaining a value for the mean metallicity of $[Fe/H] = -0.04\pm0.05$ dex. The total error including the error of the metallicity calibration, 0.15 dex, is 0.16 dex. The metallicity estimate of NGC 1245 we have obtained in this study is smaller than previous estimates, and is consistent with the radial metallicity gradient of the old open clusters, showing that the mean metallicity of NGC 1245 is not abnormally high. The reddening, distance, and age of the cluster have also been derived using the isochrones based on the convective overshooting models: the reddening $E(B-V) = 0.28\pm0.03$; the distance $d = 2.5\pm0.2 kpc$ (the corresponding galactocentric distance is RGC = 10.7 kpc, and the distance from the galactic plane is z = -0.4 kpc); and the age $t = 1.1\pm0.1 Gyrs$.

• 천문학회지
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• 제39권4호
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• pp.115-123
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• 2006

We present $JHK_S$ near-infrared CCD photometric study for the Galactic open clusters NGC 1641 and NGC 2394. These clusters have never been studied before, and we provide, for the first time the cluster parameters; reddening, distance, metallicity and age. NGC 1641 is an old open cluster with age $1.6{\pm}0.2$ Gyr, metallicity $[Fe/H]=0.0{\pm}0.2$ dex, distance modulus$(m-M)_0=10.4{\pm}0.3\;mag(d=1.2{\pm}02\;kpc)$, and reddening $E(B-V)=0.10{\pm}0.05$ mag. The parameters for the other old open cluster NGC 2394 are estimated to be $age=1.1{\pm}0.2$ Gyr, $[Fe/H]=0.0{\pm}0.2$ dex, $(m-M)_0=9.1{\pm}0.4\;mag(d=660{\pm}120\;pc)$, and $E(B-V)=0.05{\pm}0.10$ mag. The metallicities and distance values for these two old open clusters are consistent with the relation between the metallicities and the Galactocentric distances of other old open clusters. We find the metallicity gradient of 53 old open clusters including NGC 1641 and NGC 2394 to be ${\Delta}[Fe/H]/{\Delta}R_{gc}=-0.067{\pm}0.009\;dex\;kpc^{-1}$.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제27권1호
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• pp.16-23
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• 2005

It is commonly acknowledged that bone morphogenic protein (BMP-2) functions as a potential osteogenic inducer in bone formation. Recently, several papers reported that bone marrow-derived stem cell (BMSC) from human is not responsive to BMP-2 in comparison to high capacity of BMP-2 in the osteoinduction of stromal cell derived from bone marrow of rodent animals such as rat or mouse. In this study, we characterized BMSC derived from 11 years old donor for the responsiveness to rhBMP-2, dexamethasone (Dex) and 1,25-dihydroxyvitamin D (vitamin D), in order to analyze their function in the early osteogenesis. The effect of over mentioned agents was evaluated by means of assessing alkaline phosphatase (ALP) activity/staining, RT-PCR analysis and von Kossa staining. In addition, we analyzed the meaning of expressed several osteoblastic markers such as alkaline phosphatase, collagen typeI, osteopontin, bone sialoprotein and osteocalcin with relation to either differentiation or mineralization. Only in the presence of Dex, human BMSC could commit osteoblastic differentiation and matrix mineralization, and either BMP-2 or vitamin D treatment was not able to induce. But BMP-2 or Vitamin D showed potential synergy effect with Dex. ALP and bone sialoprotein were clearly expressed in response of Dex treatment compared to weak expression of osteopontin in early osteogenesis. Therefore, we expect that this study will contribute partly to elucidiating early osteogenesis mechanism in human, but variations among bone marrow donors must be considered through further study.

• 한국통신학회논문지
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• 제39B권5호
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• pp.309-316
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• 2014

안드로이드 앱(Android app, APK)을 역공학하여 디컴파일된 소스 코드를 획득하는 것이 용이하다. 공격자는 디컴파일된 소스코드를 불법적으로 사용하여 경제적 이득을 얻거나 악성코드를 삽입하여 앱을 변조하기도 한다. 이러한 문제를 해결하기 위해, 본 논문에서는 역공학 방지 방법을 사용하여 안드로이드 앱에 대한 불법 변조를 방지하는 APK 덮어쓰기 기법을 제안한다. 연구 대상은 임의 프로그래머에 의해 작성된 앱들이다. '대상 앱'(원본 앱)에 대해, 서버 시스템은 (1) 대상 앱의 복사본 생성, (2) 그 대상 앱을 암호화, (3) 복사본의 DEX (Dalvik Executable) 부분을 스텁(stub) DEX로 교체하여 스텁 앱 생성, (4) 암호화된 대상 앱 및 스텁 앱을 배포한다. 스마트폰 사용자는 암호화된 대상 앱 및 스텁 앱을 다운받는다. 스텁 앱이 스마트폰에서 실행될 때마다, 스텁 앱은 런처(launcher) 앱과 협력하여 암호화된 대상 앱을 복호화한 후 자신을 덮어쓰게 하여 원본 대상 앱이 실행되게 한다. 실행이 끝나면 복호화된 앱은 삭제된다. 제안 기법의 가능성을 검증하기 위해 여러 대중적인 앱들로 실험하여 보았다. 실험 결과, 제안 기법이 안드로이드 앱에 대해 역공학 및 변조 공격을 방지하는데 효과적임을 알 수 있다.

• Journal of Ginseng Research
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• 제39권1호
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• pp.46-53
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• 2015

Background: Glucocorticoids (GCs) are commonly used in many chemotherapeutic protocols and play an important role in the normal regulation of bone remodeling. However, the prolonged use of GCs results in osteoporosis, which is partially due to apoptosis of osteoblasts and osteocytes. In this study, effects of Korean Red Ginseng (KRG) on GC-treated murine osteoblastic MC3T3-E1 cells and a GC-induced osteoporosis mouse model were investigated. Methods: MC3T3-E1 cells were exposed to dexamethasone (Dex) with or without KRG and cell viability was measured by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Realtime polymerase chain reaction was performed to evaluate the apoptotic gene expression; osteogenic gene expression and alkaline phosphatase (ALP) activity were also measured. Western blotting was performed to evaluate the mitogen-activated protein kinase (MAPK) proteins. A GC-induced osteoporosis animal model was used for in vivo study. Results and conclusion: The MTT assay revealed that Korean Red Ginseng (KRG) prevents loss of cell viability caused by Dex-induced apoptosis in MC3T3E1 cells. Real-time polymerase chain reaction data showed that groups treated with both Dex and KRG exhibited lower mRNA levels of caspase-3 and -9, whereas the mRNA levels of Bcl2, IAPs, and XIAP increased. Moreover, groups treated with both Dex and KRG demonstrated increased mRNA levels of ALP, RUNX2, and bone morphogenic proteins as well as increased ALP activity in MC3T3-E1 cells, compared to cells treated with Dex only. In addition, KRG increased protein kinase B (AKT) phosphorylation and decreased c-Jun N-terminal kinase (JNK) phosphorylation. Moreover, microcomputed tomography analysis of the femurs showed that GC implantation caused trabecular bone loss. However, a significant reduction of bone loss was observed in the KRG-treated group. These results suggest that the molecular mechanism of KRG in the GC-induced apoptosis may lead to the development of therapeutic strategies to prevent and/or delay osteoporosis.

• 천문학회보
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• 제42권2호
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• pp.51.4-52
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• 2017

We present the derived kinematic characteristics of low-${\alpha}$ thin-disk and high-${\alpha}$ thick-disk stars in the Milky Way, investigated with a sample of about 33,900 G- and K-type dwarfs from the Sloan Extension for Galactic Understanding and Exploration (SEGUE). Based on the level of ${\alpha}$-element enhancement as a function of [Fe/H], we separate our sample into thin- and thick-disk stars and then derive mean velocity, velocity dispersion, and velocity gradients for the U, V and W velocity components, respectively, as well as the orbital eccentricity distribution. There are notable gradients in the V velocity over [Fe/H] in both populations: -23 km s-1 dex-1 for the thin disk and +44 km s-1 dex-1 for the thick disk. The velocity dispersion of the thick disk decrease with increasing [Fe/H], while the velocity.

• Journal of Advanced Research in Ocean Engineering
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• 제4권3호
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• pp.96-104
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• 2018

The importance of data management for the efficient maintenance and operation of offshore structures is becoming increasingly important. This paper has discussed the data schema and business rules that standardize the data exchange between ship design, operation and maintenance. Technical documentation that meets the international standards of ShipDex and S1000D for exchanging the operation and management data in neutral or standard formats has been introduced into the life cycle management of ships. The schema of the data exchange is represented by XML (eXtensible Markup Language) and the lifecycle data is implemented by a structured document. Lifecycle data is represented as data modules defined by XML schema. Given the feasible data generation, an example of a technical document is introduced by a general XML authoring tool.

• The Korean Journal of Physiology and Pharmacology
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• 제22권1호
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• pp.23-33
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• 2018

Cushing's syndrome (CS) is a collection of symptoms caused by prolonged exposure to excess cortisol. Chronically elevated glucocorticoid (GC) levels contribute to hepatic steatosis. We hypothesized that histone deacetylase inhibitors (HDACi) could attenuate hepatic steatosis through glucocorticoid receptor (GR) acetylation in experimental CS. To induce CS, we administered adrenocorticotropic hormone (ACTH; 40 ng/kg/day) to Sprague-Dawley rats by subcutaneous infusion with osmotic mini-pumps. We administered the HDACi, sodium valproate (VPA; 0.71% w/v), in the drinking water. Treatment with the HDACi decreased steatosis and the expression of lipogenic genes in the livers of CS rats. The enrichment of GR at the promoters of the lipogenic genes, such as acetyl-CoA carboxylase (Acc), fatty acid synthase (Fasn), and sterol regulatory element binding protein 1c (Srebp1c), was markedly decreased by VPA. Pan-HDACi and an HDAC class I-specific inhibitor, but not an HDAC class II a-specific inhibitor, attenuated dexamethasone (DEX)-induced lipogenesis in HepG2 cells. The transcriptional activity of Fasn was decreased by pretreatment with VPA. In addition, pretreatment with VPA decreased DEX-induced binding of GR to the glucocorticoid response element (GRE). Treatment with VPA increased the acetylation of GR in ACTH-infused rats and DEX-induced HepG2 cells. Taken together, these results indicate that HDAC inhibition attenuates hepatic steatosis through GR acetylation in experimental CS.