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THE EFFECT OF RHBMP-2 IN HUMAN BONE MARROW-DERIVED STEM CELLS AS OSTEOGENIC INDUCERS  

Kim, In-Sook (Dental Research Institute, Seoul National University)
Zhang, Yu-Lian (Dental Research Institute, Seoul National University)
Cho, Tae-Hyung (Dental Research Institute, Seoul National University)
Lee, Kyu-Back (Dept. of Biomedical Engineering, College of Medicine, Brain Korea 21 Program for Biomedical Science, Korea Artificial Organ Center, Korea University)
Park, Yong-Doo (Dept. of Biomedical Engineering, College of Medicine, Brain Korea 21 Program for Biomedical Science, Korea Artificial Organ Center, Korea University)
Rho, In-Sub (Chemical Engineering Department, Seoul National University of Technology)
Weber, F. (Lab. for Osteology, Dept. of Craniomaxillofacial Surgery, University Hospital Zurich)
Hwang, Soon-Jung (Dept. of Oral & Maxillofacial Surgery, College of Dentistry, Seoul National University)
Kim, Myung-Jin (Dept. of Oral & Maxillofacial Surgery, College of Dentistry, Seoul National University)
Lee, Jong-Ho (Dept. of Oral & Maxillofacial Surgery, College of Dentistry, Seoul National University)
Publication Information
Maxillofacial Plastic and Reconstructive Surgery / v.27, no.1, 2005 , pp. 16-23 More about this Journal
Abstract
It is commonly acknowledged that bone morphogenic protein (BMP-2) functions as a potential osteogenic inducer in bone formation. Recently, several papers reported that bone marrow-derived stem cell (BMSC) from human is not responsive to BMP-2 in comparison to high capacity of BMP-2 in the osteoinduction of stromal cell derived from bone marrow of rodent animals such as rat or mouse. In this study, we characterized BMSC derived from 11 years old donor for the responsiveness to rhBMP-2, dexamethasone (Dex) and 1,25-dihydroxyvitamin D (vitamin D), in order to analyze their function in the early osteogenesis. The effect of over mentioned agents was evaluated by means of assessing alkaline phosphatase (ALP) activity/staining, RT-PCR analysis and von Kossa staining. In addition, we analyzed the meaning of expressed several osteoblastic markers such as alkaline phosphatase, collagen typeI, osteopontin, bone sialoprotein and osteocalcin with relation to either differentiation or mineralization. Only in the presence of Dex, human BMSC could commit osteoblastic differentiation and matrix mineralization, and either BMP-2 or vitamin D treatment was not able to induce. But BMP-2 or Vitamin D showed potential synergy effect with Dex. ALP and bone sialoprotein were clearly expressed in response of Dex treatment compared to weak expression of osteopontin in early osteogenesis. Therefore, we expect that this study will contribute partly to elucidiating early osteogenesis mechanism in human, but variations among bone marrow donors must be considered through further study.
Keywords
BMP-2; Bone marrow-derived stem cell(BMSC); Dexamethasone(Dex); Osteogenesis; ALP(Alkaline phosphatase);
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