• Reproductive and Developmental Biology
• /
• v.37 no.4
• /
• pp.233-245
• /
• 2013

Muscle satellite cell (SC) is responsible for postnatal muscle growth, repair, and regeneration. Satellite cell is an important source of multi-potent stem cell process and differentiation into adipogenic, myogenic, and osteoblastogenic. The objective of this study was to identify alter of transcriptome during differentiation in porcine satellite cell and to elevated transcriptome at different stages of postnatal development to gain insight into the differences in differentiated PSC. We used RNA-seq technique to investigate the transcriptomes during differentiation in pig muscle. Sequence reads were obtained from Illumina HiSeq2000. Differentially expressed genes (DEG) were detected by EdgeR. Gene ontology (GO) terms are powerful tool for unification among representation genes or products. In study of GO biological terms, functional annotation clustering involved in cell cycle, apoptosis, extracellular matrix, phosphorylation, proteolysis, and cell signaling in differences stage. Taken together, these results would be contributed to a better understanding of muscle biology and processes underlying differentiation. Our results suggest that the source of DEGs could be better understanding of the mechanism of muscle differentiation and transdifferentiation.

• Journal of Life Science
• /
• v.22 no.3
• /
• pp.428-436
• /
• 2012

Protein phosphorylation is a universal mechanism that regulates cellular activities. The brassinosteroid (BR) signal transduction pathway is a relay of phosphorylation and dephosphorylation cascades. It starts with the BR-induced activation of the membrane receptor kinase brassinosteroid insensitive 1 (BRI1), resulting in the dephosphorylation of transcription factors such as BZR1/BES2 and BZR2/BES1 followed by BR-induced gene expression. Brassinosteroid signal transduction research has progressed rapidly by identifying the phosphorylation/dephosphorylation site(s) of the BR-regulated kinase and phosphatase substrates with a simultaneous pursuit of mutant phenotypes. Autophosphorylation, transphosphorylation, and serine/threonine and tyrosine phosphorylation of the receptor protein kinases BRI1 and BRI1-associated kinase (BAK1) have increased the understanding of the regulatory role of those kinases during physiological and developmental processes in plants. The phosphorylation event initiated by BR is also found in the regulation of receptor-mediated endocytosis and the subsequent degradation of the receptor. However, the basic molecular links of the BR signal transduction pathway are not well understood regarding this phosphorylation/dephosphorylation event. This review summarizes the current state of BR signal transduction research to uncover the phosphorylation/dephosphorylation networks and suggests directions for future research on steroid signal transduction to gain a more comprehensive understanding of the process.

• Korean Journal of Ecology and Environment
• /
• v.41 no.1
• /
• pp.90-97
• /
• 2008

The aim of this research was to detect and observe delivering generation effects from $F_0$ generation exposed to 4-nonylphenol 4NP) to $F_1$ generation on Tigriopus japonicus. The nauplius survival rate of $F_1$ from $F_0$ exposed to low 4NP concentration was significantly lower than other concentrations. Among the developmental process, copepodite first emergence day (CE) and adult male first emergence day (ME) on $F_1$ were accelerated relatively high concentration conditions. The sex ratio of $F_1$ was different between control group and treatment groups for 1, 10 and $30{\mu}g\;L^{-1}$ 4NP. The fecundity of $F_1$ was not significantly affected (except, $0.1{\mu}g\;L^{-1}$ 4NP). When 4NP concentrations were increased, first brooding day (FB) of F 1 was generally delayed. The length, width, and biomass of $F_1$ adult female were smaller than control group. In addition, the width and biomass of adult male were narrower or smaller than control group.

• Clinical and Experimental Reproductive Medicine
• /
• v.24 no.2
• /
• pp.233-239
• /
• 1997

Embryos of most mammalian species grown in vitro would undergo developmental arrest at the approximate time of genomic activation. Stage-specific cell block and the resulting rapid loss of embryo viability in conventional culture media have limited the duration for which embryos may be cultured prior to transfer. As a result, embryos are usually transferred to the uterus at the 4-to 8-cell stage to avoid the loss of viability associated with long-term in vitro culture. Early transfer has led to asynchrony of the endometrium-trophectoderm interaction at the time of implantation and a resultant reduction in the rate of implantation. To overcome these problems, a variety of co-culture systems has been devised in which embryos can develop for a longer period prior to embryo transfer. Vero cells, derived from African green monkey kidney, share a common embryologic origin with cells from the genital tract. In addition, they are potentially safe to use, since they are highly controlled for viruses and other contaminants. Therefore, co-culture using Vero cells has been widely utilized to enhance embryo viability and development, although not without controversies. We thus designed a series of experiments to demonstrate whether Vero cells do indeed enhance mouse embryo development as well as to compare the efficacy of co-culturing mouse 1-cell embryos on Vero cell monolayer in both Ham's F-10 and human tubal fluid (HTF) culture media. 1-cell stage ICR mouse embryos were cultured either in the presence of Vero cells (Group A) or in conventional culture medium alone (Group B). In Ham's F-10 significantly more 3-to-8cell embryos developed in group A than group B (59.8 versus 10.0%; p<0.01). In contrast, there was no significant difference in embryonic development both group A and group B in HTF. However, significant differences were noted only in later embryonic stage (13 and 0%; p<0.05 of group A and B respectively, hatching or hatched). In Ham's F-10, we also could observe the beneficial effect of Vero cell on hatching process (70.7 and 42.1%; p<0.05 of group A and group B respectively).

• Clinical and Experimental Pediatrics
• /
• v.64 no.10
• /
• pp.504-510
• /
• 2021

Population screening of newborns is an extremely important and informative diagnostic approach that allows early identification of babies who are predisposed to the development of a number of serious diseases. Some of these diseases are known and have effective treatment methods. Neonatal screening enables the early diagnosis and subsequent timely initiation of therapy. This helps to prevent serious complications and reduce the percentage of disability and deaths among newborns and young children. Primary immunodeficiency diseases and primary immunodeficiency syndrome (PIDS) are a heterogeneous group of diseases and conditions based on impaired immune system function associated with developmental defects and characterized by various combinations of recurrent infections, development of autoimmune and lymphoproliferative syndromes (genetic defects in apoptosis, gene mutation Fas receptor or ligand), granulomatous process, and malignant neoplasms. Most of these diseases manifest in infancy and lead to serious illness, disability, and high mortality rates. Until recently, it was impossible to identify children with PIDS before the onset of the first clinical symptoms, which are usually accompanied by complications in the form of severe coinfections of a viral-bacterial-fungal etiology. Modern advances in medical laboratory technology have allowed the identification of children with severe PIDS, manifested by T- and/or B-cell lymphopenia and other disorders of the immune system. This review discusses the main existing strategies and directions used in PIDS screening programs for newborns, including approaches to screening based on excision of T-cell receptors and kappa-recombination excision circles, as well as the potential role and place of next-generation sequencing technology to increase the diagnostic accuracy of these diseases.

• Animal Bioscience
• /
• v.34 no.6
• /
• pp.1078-1087
• /
• 2021

Objective: Skeletal muscle satellite cells (SMSCs) are significant for the growth, regeneration, and maintenance of skeletal muscle after birth. However, currently, few studies have been performed on the isolation, culture and inducing differentiation of goose muscle satellite cells. Previous studies have shown that C1q and tumor necrosis factor-related protein 3 (CTRP3) participated in the process of muscle growth and development, but its role in the goose skeletal muscle development is not yet clear. This study aimed to isolate, culture, and identify the goose SMSCs in vitro. Additionally, to explore the function of CTRP3 in goose SMSCs. Methods: Goose SMSCs were isolated using 0.25% trypsin from leg muscle (LM) of 15 to 20 day fertilized goose eggs. Cell differentiation was induced by transferring the cells to differentiation medium with 2% horse serum and 1% penicillin streptomycin. Immunofluorescence staining of Desmin and Pax7 was used to identify goose SMSCs. Quantitative realtime polymerase chain reaction and western blot were applied to explore developmental expression profile of CTRP3 in LM and the regulation of CTRP3 on myosin heavy chains (MyHC), myogenin (MyoG) expression and Notch signaling pathway related genes expression. Results: The goose SMSCs were successfully isolated and cultured. The expression of Pax7 and Desmin were observed in the isolated cells. The expression of CTRP3 decreased significantly during leg muscle development. Overexpression of CTRP3 could enhance the expression of two myogenic differentiation marker genes, MyHC and MyoG. But knockdown of CTRP3 suppressed their expression. Furthermore, CTRP3 could repress the mRNA level of Notch signaling pathway-related genes, notch receptor 1, notch receptor 2 and hairy/enhancer-of-split related with YRPW motif 1, which previously showed a negative regulation in myoblast differentiation. Conclusion: These findings provide a useful cell model for the future research on goose muscle development and suggest that CTRP3 may play an essential role in skeletal muscle growth of goose.

• Journal of the Korea Convergence Society
• /
• v.12 no.12
• /
• pp.371-388
• /
• 2021

This research was designed to provide the materials helpful for art therapy education and art therapist training by exploring the intervention processes and professional competency factors of art therapists which facilitate therapeutic alliance through the art therapists' actual clinical experiences. To this end, in-depth interviews were conducted with 9 art therapists, and analysis of the materials was divided into 5 sections and 41 categories using Consensus Qualitative Analysis (CQR). According to study results, the art therapist's intervention process for the promotion of therapeutic alliance signifies the structuralization of a safe environment through basic items of consideration for a therapeutic alliance and the three dimensions of its composition, and the art therapist's professional capacity interacts with the art therapists intervention factor to directly and indirectly affect the therapeutic alliance. Subsequently, it is essential for art therapists to reinforce their professional abilities relevant to conceptualizing unique individual cases with clinical problems, sufficient clinical experiences, the ability to utilize art as therapeutic treatment, and efforts to maintain professional capabilities for therapeutic purposes. The significance of this research lies in presenting the materials to be used in educating and training the art therapists by deriving the developmental tasks necessary for structuralizing the facilitation of the therapeutic alliance and strengthening the professional expertise of the art therapist.

• Journal of Korean Society of Forest Science
• /
• v.110 no.4
• /
• pp.479-495
• /
• 2021

To improve on the efficient management of forest resources, it is necessary to create a forest soil map, which represents a comprehensive database of forest lands. Although a 1:25,000 scale forest site map has been used in Korea, the need for a large-scale forest soil map with high precision and information on forest lands that is specialized for individual purposes has been identified. Moreover, to keep pace with the advancement in forest management and transition to a digital society, it is essential to develop a method for constructing new forest soil maps that can diversify its use. Therefore, this paper presented a developmental process and used a 1:5,000 scale forest soil map to propose future directions. National maps showing the soil type, depth, and texture were produced based on the survey and analysis of forest soils, followed by the Forest Land Soil Map (1:5,000) Production Standard Manual. Alternatively, forest soil map data were the basis on which various other maps that can be used to prevent and predict forest disasters and evaluate environmental capacities were developed. Accordingly, ways to provide appropriate information to achieve the national forest plan, secure forestry big data, and accomplish sustainable forest management that corresponds to the national development plan are proposed based on results from the current study.

• Korean Journal of Comparative Education
• /
• v.28 no.3
• /
• pp.243-263
• /
• 2018

In order to confirm the academic authenticity of comparative education, the academic methodology which is also appropriate for the historical arrangement is the history. In this sense, this article focuses on the study of comparative education from the viewpoint of history of education and aims for a tentative approach to comparative education history. In every discipline, it is essential to organize the history of the discipline both for those majoring in it and for the development of the discipline itself. The conclusions obtained from this study are as follows. First, in general, the method of studying comparative education includes not only 'comparative studies' but also 'regional studies', but it can add academic depth through the study of time series called 'comparative education history'. Second, the developmental process of the comparative education can be classified into four stages; the first stage is 'the pre-history' of the comparative education, the second stage is the 'embryo stage', the third stage is the 'establishment stage', and the fourth stage is the 'development stage'. Especially, in the era of globalization, the academic potential of comparative education is even higher, so it is possible to add an 'expanding period'. Third, it is necessary to understand the 'comparative education history' in order to secure the academic identity of the comparative education study. It is expected that it will be possible to more easily confirm the perspectives and issues of comparative education in the future, together with a good conceptual understanding of comparative education.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.20 no.6
• /
• pp.448-459
• /
• 2019

The purpose of this study is to investigate the differences between children's internal variables (gender, temperament, development) and environmental variables (teaching efficacy, teaching - child interaction, classroom environment) The data collected for this study were analyzed using descriptive statistics, reliability analysis, correlation analysis, cluster analysis, and t-test using the SPSS 22.0 program. The results are as follows. First, the peer interaction of the infant showed a difference in sex between the types. Second, children's peer interaction showed differences in interstitial temperament, language development, and cognitive development. Third, the peer interaction of young children was different between the types of environment variables such as teaching efficacy, teacher - infant interaction, and classroom environment. As a result, it is suggested that the children's social temperament will lead to healthy peer interaction, and that language development and cognitive development will lead to a positive developmental process. In this study, the meaning and meaning of children's intergenerational behaviors in children's gender and temperament, language and cognitive development, and environmental variables such as teaching efficacy, teacher - infant interaction, I checked. In addition, it is meaningful that the positive and negative peer interactions are segmented and analyzed in detail to examine the peer interaction of infants. However, the limitation of this study is that it is not possible to investigate all the fields belonging to the infant's personal variables and environmental variables.