• International Journal of Advanced Culture Technology
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• v.10 no.2
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• pp.135-139
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• 2022

The purpose of this study is to explore the application structure of customized "lifelong education: (versus) higher education" for individuals with developmental disabilities in two types.As for the research method, expert consultation was composed secondary with literature analysis as the primary procedure.The contents of the study were presented by classifying the application structure of customized lifelong education versus higher education for individuals with developmental disabilities into two types. Accordingly, the first research content is a school type-centered structure, which can be understood as a type that focuses on higher education while linking aspects of lifelong education. And the second research content is a structure centered on the type of independent life of individuals with developmental disabilities, which can be understood as a type that focuses on lifelong education while linking with the aspects of higher education. As a result of the study, the aspect that the research contents considered above can be gradually realized through the currently established infrastructure at Daegu University in the current situation in Korea has been improved.

• International Journal of Advanced Culture Technology
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• v.10 no.1
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• pp.24-35
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• 2022

The purpose of this study was to form a linked model in which local institutions related to lifelong education for the disabled can cooperate based on the Daegu University K-PACE Center. The contents of the study started with recognizing the problem that the adult-centered lifelong education support system does not effectively cope with these factors, even though the independent life of people with developmental disabilities is a major factor determining the quality of life. Regarding this problem recognition, this study primarily emphasized the view that educational support for independent life of people with developmental disabilities should establish the context of the school foundation. The context of the school foundation is established for lifelong education centered on adulthood for people with developmental disabilities because the curriculum is embodied through the standards of subject matter education. In this regard, the Daegu University K-PACE Center, which established a curriculum that supports the independent life of people with developmental disabilities in terms of linking higher and lifelong education, actually reflects the context of the school foundation. As a result, this study prepared a strategy that could be considered as a transition to advance the curriculum organized by the Daegu University K-PACE Center, and the strategy was secondarily reflected as a procedure that could be linked to local lifelong education-related institutions for the disabled. Finally, this study presented a form of transition in which people with developmental disabilities can access the curriculum of lifelong education through the connection of local lifelong education-related institutions for the disabled, centering on the entire life of adulthood.

• Journal of Embryo Transfer
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• v.12 no.1
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• pp.27-36
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• 1997

The studies were carried out to investigate the effects of co-culture with cumulus cells and oviduct epithelial cells on the in vitro fertilization and cleavage rate of bovine follicular cocytes and to determine the optimum thawing temperature and equilibration time on in vitro developmental rate of frozen bovine embryos. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes were cultured in TGM-199 medium containing 10 IU /ml의 PM SG, 10 IU /ml의 hCG, ip g/ml의 $\beta$-estradiol and 10% FCS for 24~48 hrs in incubator with 5% $CO_2$ in air at 38.5$^{\circ}C$. The bovine embryos following dehydration by cryoprotective agents and a various concentration of sucrose were directly plunged into liquld nitrogen and thawed in 3$0^{\circ}C$ water. Survival rate was defined as developmental rate on in vitro culture or FDA-test. The results are sunanarized as followes :1. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with cumulus cells in TCM499 medium were 75.0~76.8% and 17.3~27.6%, respect-ively. And in-vitro fertilization rates of cumulus-enclosed oocytes(55.4%)were significantly(p<0.05) higher than cumulus-denuded oocytes (23.1%). 2. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with l$\times$ l04cells /ml, 1 x l06cells /ml, lx l08cells /ml and 1 x l015cells /ml oviduct epithelial cells in TCM-199 medium were 74.5~77.8% and 15.7~21.20 respectively.3. The in-vitro fertilization and in vitro developmental rates of bovine oocytes cocultured in '1CM-199 media containing PMSG, hCG, PMSG+hCG. PMSG+$\beta$-estradiol, hCG+$\beta$-estradiol 0 to 40 hrs after insemination were 74.0~77.4% and l8.9~23.l%, re-spectiv ely.4.The survival rates of bovine embryos thawed after rapid freezing in the freezing medium containing a various concentration of sucrose added 1.5M and 2.OM glycerol,DMSO and propanediol were 23.5~31.4% and 20.6~34.l%, respectively. 5. The temperature thawed at 3$0^{\circ}C$ after rapid freezing of bovine embryos resulted in a significantly higher embryos survival rate than did at 2$0^{\circ}C$ and 35$^{\circ}C$.6. The equilibration time on the survival rates of bovine embryos was attained after short period of time(2.5~5 min.) in the freezing medium higher than long period of time (10~20min.). (Key words : bovine embryos, co-culture, freezing, in vitro development)

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.8
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• pp.1190-1195
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• 2007

This study was conducted to improve efficiency of a follicle culture system without reducing developmental competence of intrafollicular oocytes. Preantral follicles (100 to $125{\mu}m$ in diameter) of F1 hybrid (B6CBAF1) mice were cultured singly for 216 h in modified ${\alpha}$-MEM-glutamax medium, to which 2.5 IU/ml hCG and epidermal growth factor was added 16 h prior to the end of culture. Medium change was either performed three times (54 h interval), twice (72 h interval), once (108 h interval), or not at all (216 h interval). Maturation (progression to the metaphase II stage) of intrafollicular oocytes was detected from 4 days after culture in the three-times change treatment, while all treatments yielded mature oocytes from day 5 of culture. Compared with the three-times change, decreasing the change frequency to once did not reduce the capacity to begin maturation (germinal vesicle breakdown of 82 to 86%), to mature (78 to 79%) and to develop into blastocysts after parthenogenetic activation (29 to 32%). Morphological parameters were similar among these treatments. Except for the no medium change treatment, similar colony-forming activity of inner cell mass cells after culturing of blastocysts in leukemia inhibitory factor-containing medium was detected, while the morphology of the colony-forming cells deteriorated in the change-once treatment compared with the change twice or three-times. In conclusion, the efficiency of the preantral follicle culture system could be improved by reducing frequency of medium change up to a 72 h interval (three times in total 216 h culture) without decreasing developmental competence of oocytes.

• Reproductive and Developmental Biology
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• v.28 no.4
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• pp.253-256
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• 2004

Porcine fibroblasts were transferred into enucleated bovine oocytes for the interspecies nuclear transfer (NT). After NT, the embryos were cultured in three different culture systems. The media used for the experiment were CR1aa and NCSU23. The culture systems used for the experiment were: 1. Culture in CR1aa for 7 days (CR). 2. Culture in CR1aa for 2 days and subsequently in NCSU23 for 5 days (CR-NC). 3. Culture in NCSU23 for 7 days (NC). Bovine (intraspecies) NT group was used as a control. The oocytes in bovine NT group were treated the same as interspecies NT embryos except using bovine fibroblasts as nuclear donors. Regardless of their nuclear origin (interspecies vs bovine), the embryos in CR (68.4% vs 77.2%) and CR-NC (67.8% vs 70.5%) showed better developmental competence to the 2-cell stage (p<0.05) than those in NC (41.0% vs 10.0%). Bovine NT embryos in CR-NC did not develop over the 4-cell stage after the medium replacement, while interspecies NT embryos in CR-NC continued to develop and could reach over the 8-cell stage (12.2%). Blastocysts were only found in bovine NT group (17.4%), but no blastocyst was found in interspecies NT group. This study suggests that the development of interspecies NT embryos mostly depends on their recipient cytoplasm during the culture in vitro.

• Journal of the Korean Society of School Health
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• v.10 no.2
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• pp.137-156
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• 1997

Most education materials on sexuality developed in the past 10 years have not been thoroughly evaluated. This study provides results from a content analysis of 5 education materials for primary, middle and high school student. The results of this study were as follows: The Analysis Framework was developed to measure content in the education materials. It was organized into six domains. They are "Human Development", "Relationship", "Personal Skills", "Sexual Behavior", "Sexual Health", "Society and Culture". Each was associated topics and age appropriate developmental guides. The standard for analysis was the developmental guide suggested by the analysis framework. Each education material was assessed for developemtal guidance and its appropriateness for primary, middle and high school students. The total number of "being" responses to each developmental guidance equaled the score the material earned. These scores then were judged as to how well each education materials was comprehensive. 3 of 5 education materials were addressed as less than half the developmental guidline. Certain education materials were less comprehensive than others. I want to know 'Sexuality' covered the fewest developmental guidliness (34.6%). Interstingly, certain domains received much more coverage across all education materials than others. Most education materials provided instruction concerning "Human Development" (62.6%), "Sexual Health" (61.0%). However, topics such as "Personal Skills" (16.2%), "Relationship" (44.1%), "Sexual Behavior" (46.8%), "Society and Culture" (48.6%) were rarely addressed. Three topic, "negotiation", "sexual fantasy", "sexual dysfunction", were never addressed by most of the materials. Because several of the education material contained gender and sexual orientation biases. Certain topics such as "sexual identity and orientation" and "Gender role" were not adequately addressed by most of the material. Unfortunately, the overall findings of this study revealed that most of the education materials were considered insufficient for students. Because this study focused on whether or not the education materials addressed the developmental guidlines, further research is recommended to evaluate and addressed each developmental guidline.

• Journal of Embryo Transfer
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• v.11 no.3
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• pp.201-210
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• 1996

This experiment was carried out to evaluate the effect of early mouse embryonic development in vitro by co-culture with bovine and porcine oviductal epithelial cells (BOEC and POEC). The 2-cell embryos were collected from the oviducts of the superovulated and mated cultured in D-PBS /15% FCS at 48 hours after hCG injection. The in vitro developmental rate of blastocyst formation in the embryos were examined under the fllowing treatments; 1) TCM 199 added 15% HCS, 2) Ham's F-10 added 15% HCS, 3) MediCult IVF medium, 4) TCM 199 added 15% HCS + BOEC, 5) TCM 199 added 15% HCS + POEC, 6) Ham's F40 added 15% HCS + BOEC, 7) Ham's F-10 added 15% HCS + POEC,8) MediCult IVF medium + BOEC, 9) MediCult IVF medium + POEC. For a comparative study of in vitro development for 96 hours after hCG injection, were cultured with oviductal epithelial cell and media only. The obtained results were 2-cell embryos developed to the blastocyst stage in TCM 199, Ham's F-10 and MediCult IVF medium at the rates of 84.4,83.2 and 81.6%. respectively. The higher developmental rates(91~97%) of blastocyst formation was appeared when the embryos were co-cultured with a monolayer of bovine or porcine oviductal epithelial cells in TCM 199 or Ham's F-10 and MediCult IVF media. No significant difference in developmental rates was shown between bovine and porcine oviductal epithelial cells but significant difference in co-culture system in comparison between media only system and co-cultures. In conclusions, oviductal epithelial cells, BOEC and POEC, when co-culture with mouse early embryos improved the rates of development, blastocyst and hatching. Therefore, it is suggested that co-culture system using oviductal epithelial cells improve early embryonic developtnent in mouse.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2005.10a
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• pp.87-88
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• 2005

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2002.11a
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• pp.107-107
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• 2002

• Journal of Environmental Health Sciences
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• v.31 no.4 s.85
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• pp.254-259
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• 2005

Permethrin, a synthetic pyrethroid insecticide, has been widely used to protect domestic animals and the public health, as well as in agriculture against a variety of pests, which provides potential for environmental exposure. Permethrin is classified as possible human carcinogen and endocrine disrupting chemical by many international authorities. However, its developmental effects have been rarely studied. This study investigated the effects of permethrin during embryo-genesis. Developmental toxicity of permethrin was evaluated using short-term in vitro battery system. Gestation day 9.5 rat embryos (organogenesis) were cultured with permethrin (0.1,0.4 and 0.8 mg/ml) for 48 hours using whole embryo culture system. All the treatments exhibited significant decreases in the total morphological score. Permethrin induced significant growth retardation and the developmental abnormality at doses of 0.4 and 0.8 mg/ml. Moreover, the DNA and protein contents of embryos decreased in dose-dependent manner. These observations suggest that permethrin contributes to toxicity on embryonic developments in rats.