• 제목/요약/키워드: Development stage

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Molecular Genetics of Emericella nidulans Sexual Development

  • Han, Kap-Hoon
    • Mycobiology
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    • 제37권3호
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    • pp.171-182
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    • 2009
  • Many aspergilli that belongs to ascomycetes have sexuality. In a homothallic or self-fertile fungus, a number of fruiting bodies or cleistothecia are formed in a thallus grown from a single haploid conidia or ascospores. Genome-sequencing project revealed that two mating genes (MAT) encoding the regulatory proteins that are necessary for controlling partner recognition in heterothallic fungi were conserved in most aspergilli. The MAT gene products in some self-fertile species were not required for recognition of mating partner at pheromone-signaling stage but required at later stages of sexual development. Various environmental factors such as nutritional status, culture conditions and several stresses, influence the decision or progression of sexual reproduction. A large number of genes are expected to be involved in sexual development of Emericella nidulans (anamorph: Aspergillus nidulans), a genetic and biological model organism in aspergilli. The sexual development process can be grouped into several development stages, including the decision of sexual reproductive cycle, mating process, growth of fruiting body, karyogamy followed by meiosis, and sporulation process. Complicated regulatory networks, such as signal transduction pathways and gene expression controls, may work in each stage and stage-to-stage linkages. In this review, the components joining in the regulatory pathways of sexual development, although they constitute only a small part of the whole regulatory networks, are briefly mentioned. Some of them control sexual development positively and some do negatively. Regarding the difficulties for studying sexual differentiation compare to asexual one, recent progresses in molecular genetics of E. nidulans enlarge the boundaries of understanding sexual development in the non-fertile species as well as in fertile fungi.

토기에서 핵이식 수정란의 초기 발달 속도와 난자 활성화가 후기배로의 발달에 미치는 영향 (Effect of Early Stage of Reconstituted Embryos with or without Oocyte Preactivation on Subsequent In Vitro Development of Nuclear Transplant Rabbit Embryos)

  • 전병균;윤희준;공일근;이효종;박충생
    • 한국수정란이식학회지
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    • 제12권1호
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    • pp.1-10
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    • 1997
  • The present study was conducted to investigate the influence of embryo cell stage at 18h post-fusion and oocyte preactivation on sebsequent in vitro developmental potential in the nuclear transplant rabbit embryos. The embryos of 16-cell stage were collected and synchronized to G$_1$ phase of 32-cell stage. The recipient cytoplasms were obtained by removing the first polar body and chromosome rnass from the oocytes collected by non-dis-ruptive microsurgery procedure. The separated G$_1$ phase blastomeres of 32-cell stage were injected into non-preactivated recipient cytoplasms. Otherwise, the enucleated recipient cytoplasms were preactivated by electrical stimulation at 18h post-hCG injection and the separated G$_1$ phase blastomeres of 32-cell stage were injected. Mter culture until 20h post-hOG injection, the nuclear transplant oocytes were electrofused by electrical stimulation. The fused nuclear transplant embryos were classified into 3~4-cell, 2-cell and 1-cell stage at 18 hrs post-fusion and cultured until the embryos reached blastocyst stage. The developmental rate to blastocyst stage was significantly (P <0.05) higher in all the reconstituted embryos of 3~4-cell stage(58.0%) than in 2 and icell stage. The developmental rate to blastocyst stage in the embryos of 3~4-cell stage at 18 hrs post-fusion was significantly (P<0.05) higher in the reconstituted without oocyte preactivation(77.8%) than in the oocyte-preactivated embryos (33.3%). These results indicated that the higher rate of in the in vitro development to blastocyst stage might be obtained form the embryos which were reconstituted with nuclear donor of G$_1$ phase and non-preactivated oocyte, and developed more rapidly for 18 hrs post-fusion.

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흰점독가시치 (Siganus canaliculatus)의 생식소 발달 및 생식주기 (Gonadal Development and Reproductive Cycle of the Rabbitfish (Siganus canaliculatus))

  • 황형규;박창범;강용진;이종하;노섬;이영돈
    • 한국수산과학회지
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    • 제37권5호
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    • pp.393-399
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    • 2004
  • Annual reproductive cycle of Siganus canaliculatus was studied based on monthly variation of gonadosomatic index (GSI) and histological changes of gonads. Samples were monthly collected by a set net along the southern coast of Jeju Island, Korea from January to December, 1996. Variation of the monthly mean GSI values showed similar trends in female and male. The GSI values increased from June and reached a peak in the spawning season in July $(9.65{\pm}1.96\;in\;females,\;10.00{\pm}4.27\;in\;males)$, and decreased rapidly thereafter. Female hepatosomatic index (HSI) values ranged from $1.26{\pm}0.22\;(in\;April)\;to\;2.34{\pm}0.39$ (in July), and male HSI values ranged from $1.27{\pm}0.21\;(in\;April)\;to\;1.87{\pm}0.30$ (in October). Annual reproductive cycle was classified into the following successive stages: in female, growing stage (from February to June), mature stage (from June to July), ripe and spawning stage (from July to August), recovery stage (from August to March); and in male, growing stage (from January to June), mature stage (from June to July), ripe and spent stage (from July to August), and recovery stage (from August to April). Based on these data, this species has a group-synchronous oocyte development and one spawning season a year.

Changes of Methyl trans-cinnamate Levels During Fruit-body Development in Tricholoma matsutake

  • Lee, Wi Young;Park, Youngki;Ahn, Jin Kwon;Ka, Kang Hyeon
    • 한국산림과학회지
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    • 제94권5호통권162호
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    • pp.330-333
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    • 2005
  • The relationship between the contents of methyl trans-cinnamate and the ratio of DP/DS (diameter of pileus / diameter of stalk) in the fruit-body of Tricholoma matsutake during its development was investigated. The stages of development were divided as follows: stage A is less than 1, stage B is from 1 to less than 2, stage C is from 2 to less than 3, and stage D is more than 3 of the values of DP/DS. The contents of methyl trans-cinnamate in pileus and stalk of pine mushroom ranged from $77{\mu}g/g$ to $824{\mu}g/g$ and from $7.6{\mu}g/g$ to $22.4{\mu}g/g$, respectively during its development. In the part of pileus, there is no relevance of the methyl trans-cinnamate content of pine mushroom between the stage A and B, but there was significantly different among the stage of B, C and D. In the case of stalk, the relevance of the methyl trans-cinnamate content of pine mushroom between stage D and other stages showed a low difference. In addition, as pileus of pine mushroom developed the level of the aroma compound increased as well and showed higher correlation relationship ($r^2=0.877$) between the contents of methyl trans-cinnamate in the pileus and the ratio of DP/DS. From the results of this study, we can conclude that the aromatic component of pine mushroom can be deduced from the value of DP/DS, which indicates the stage of the development appearance.

가족발달단계에 따른 간호요구영역에 관한 연구 (Study of The Area of Nursing Need by the Family Developmental Stage)

  • 최부옥
    • 대한간호학회지
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    • 제7권2호
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    • pp.43-59
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    • 1977
  • The Community Health Service considers the family as a service unit and places the emphasis of its service on the health problems and the nursing needs of the family rather than the individual. From the conceptual point of view that tile community health service is both health maintenance and health promotion of the family, the community health nurse should have a knowledge of the growth and development of the family and be responsible for the comprehensive support of normal family development. The community health nurse often is in a position to make a real contribution to normal family development. In order to investigate the relationship between the areas of nursing need and family development, the following objectives were established 1. To discover the general characteristics of the study population by the stage of family development. 2. To discover specific nursing needs in relation to the family developmental stage, and to determine the intensity of the nursing needs and the ability of the family to cope with these needs. 3. To discover overall family health nursing problems in relation to the family developmental stage and determine the intensity of the nursing need and the problem solving ability of family. Definitions : The family developmental stages as classified by Dually were used stage 1. Married couples(without children) stage 2. Childbearing Families (oldest child birth to 30 months of age) stage 3. Families with preschool children (oldest child 2½-to 6 years) stage 4. Families with schoolchildren (oldest child 6 to 13 years). stage 5. Families with teenagers (oldest child 13 to 20 years) stage 6. Families as launching centers (first child gone to last child′s leaving home). stage 7. Middle- aged parents (empty nest to retirement) stage 8. Aging family member (retirement to death of both spouses) The areas of nursing need were defined as those used in the study, "A Comprehensive Study about Health and Nursing Need and a Social Diagram of the Community", by tile Nursing research Institute and Center for population. and Family Planning, July 1974. The study population defiled and selected were 260 nuclear families ill two myron of Kang Hwa Island. Percent, mean value and F- test were utilized in tile statistical analysis of the study result. Findings : 1. General characteristics of the study population by tile family developmental stage ; 1)The study population was distributed by the family developmental stage as follows : stage 1 : 3 families stage 2 : 13 families stage 3 : 24 families stage 4 : 41 families stage 5 : 50 families stage 6 : 106 families stage 7 : 13 families stage 8 : 10 families 2) Most families had 4 or 5 members except for those in stage, 1, 7, and 8. 3) The parents′ present age was older in the higher developmental stage and their age at marriage was also younger in the higher developmental stages. 4) The educational level of parents was primarily less than elementary school irrespective of the developmental stage. 5) More than half of parents′ occupations were listed as laborers irrespective of the developmental stage, 6) More than half of the parents were atheists irrespective of the developmental stage. 7) The higher the developmental stage(from stage 2 to stage 6 ), the wider the distribution of children′s ages. 8) More than half of the families were of middle or lower socio-economic level. 2. Problems in specific areas of nursing need by family developmental stage, the intensity of nursing need and the problem solving ability of the family : 1) As a whole, many problems, irrespective of the developmental stage, occurred in tile areas of Housing and Sanitation, Eating Patterns, Housekeeping, Preventive Measures and Dental care. Problems occurring ill particular stages included the following ; stage 1 : Prevention of Accident stage 2 : Preventive Vaccination, Family Planning. stage 3 : Preventive Vaccination, Maternal Health, Family Planning, Health of Infant and Preschooler. stage 4, 5 : Preventive Vaccination, Family Planning, Health of School Children. stage 6 : Preventive Vaccination, Health of School Children. 2) The intensity of the nursing need in the area of Acute and Chronic Diseases was generally of moderate degree or above irrespective of the developmental stages except for stage 1. Other areas of need listed as moderate or above were found in the following stages: stage 1 : Maternal Health stage 3 . Horsing and Sanitation, Prevention of Accident. stage 4 . Housing and Sanitation. stage 5 : Housing and Sanitation, Diagnostic and Medical Care. stage 6 : Diagnostic and Medical care stage 7 : Diagnostic and Medical Care, Housekeeping. stage 8 : Housing and Sanitation, Prevention of Accident, Diagnostic and Medical Care, Dental Care, Eating Patterns, Housekeeping. 3) Areas of need with moderate problem solving ability or less were as follows : stage 1 : Diagnostic and Medical Care, Maternal Health. stage 2 : Prevention of Accident, Acute and Chronic Disease, Dental Care. stage 3 : Housing and Sanitation, Acute and Chronic Disease, Diagnostic and Medical Care, Preventive Measure, Dental Care, Maternal Health, Health of Infant and preschooler, Eating Patterns. stage 4 : Housing and Sanitation, Prevention of Accident, Diagnostic and Medical Care, Preventive Measure, Dental Care, Maternal Health, Health of New Born, Health of Infant and Preschooler, Health of school Children, Eating Patterns, Housekeeping. stage 5 . Housing and Sanitation, Prevention of Accident, Acute and Chronic Disease, Diagnostic and Medical Care, Preventive Measure, Dental Care, Preventive Vaccination, Maternal Health, Eating Patterns. stage 7, 8 : Housing and Sanitation, Prevention of Accident, Acute and Chronic Disease, Diagnostic and Medical Care, Preventive Measures, Dental Care, Preventive Vaccination, Eating Patterns , Housekeeping. Problem occurrence, the degree of nursing need and the degree of problem solving ability 1 nursing need areas for the family as a whole were as follows : 1) The higher the stages(except stage 1 ), the lower the rate of problem occurrence. 2) The higher the stage becomes, the lower the intensity of the nursing need becomes. 3) The higher the stages (except stages 7 and 8), the higher. the problem solving ability. Conclusions ; 1) When the nursing care plan for the family is drawn up, depending upon the stage of family development, higher priority should be give to nursing need areas ① at which problems were shown to occur ② where the nursing need is shown to be above moderate degree and ③ where the problem solving ability was shown to be of moderate degree. 2) The priority of the nursing service should be Placed ① not on those families in the high developmental stage but on those families in the low developmental stage ② and on those areas of need shown in stages 7 and 8 where the degree nursing need was high and the ability to cope low.

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Attitude Controller Design and Test of Korea Space Launch Vehicle-I Upper Stage

  • Sun, Byung-Chan;Park, Yong-Kyu;Roh, Woong-Rae;Cho, Gwang-Rae
    • International Journal of Aeronautical and Space Sciences
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    • 제11권4호
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    • pp.303-312
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    • 2010
  • This paper introduces the upper stage attitude control system of KSLV-I, which is the first space launch vehicle in Korea. The KSLV-I upper stage attitude control system consists of two electro-hydraulic actuators and a reaction control system using cold nitrogen gas. A proportional, derivative, and integral controller is designed for the electro-hydraulic thrust vectoring system, and Schmidt trigger ON/OFF controllers are designed for the reaction control system. Each attitude controller is designed to have enough stability margins. The stability and performance of KSLV-I upper stage attitude control system is verified via hardware in the loop tests. Hardware in the loop tests are accomplished for perturbed flight conditions as well as nominal flight condition. The test results show that the attitude control loop of KSLV-I upper stage is very stable and the attitude controllers perform well for all flight conditions. Attitude controllers designed in this paper have been successfully applied to the first flight of KSLV-I on August 25, 2009. The flight test results show that all attitude controllers of the KSLV-I upper stage performed well and satisfied the accuracy specifications even during abnormal flight conditions.

농업인력의 발전단계별 체계적인 육성방안 연구 (A Study on Policies for Systematically Fostering Eligible Farmers based on Their Developing Stages)

  • 서규선
    • 현장농수산연구지
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    • 제4권1호
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    • pp.3-28
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    • 2002
  • This study was carried to devise comprehensive policies for systematically fostering the eligible farmers in Korea based on their vocational developing stages such as potential stage in juvenile days, preparing stage in agricultural schools and colleges, farm worker stage under farm manager, ownership farming stage and professional farm manager stage. Of course all of these developing stages are not necessary but desirable processing to be a good farmer. At least more than two stages are necessary to be a professional farm manager. In order to systematically foster the eligible farmers as many as demanded in Korea, appropriate policies in the every stage should be devised. As the result of this study, several programs were sugested as the appropriate policies such as development of school 4-H program in potential stage, scholarship program in preparing stage, supporting programs for the farmhouse-hold having a family's farm management agreement, development of the instalment savings program for the young farm workers and farmer's certificate program, etc.

생쥐배의 발생단계별 미세분할, 배양 및 이식 관한 연구 (Studies on Culture and Transfer of Mouse Embryos Biseeted at Various Cell Stages)

  • 강대진;박희성;이효종;박충생
    • 한국수정란이식학회지
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    • 제4권1호
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    • pp.28-34
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    • 1989
  • These experiments were carried out to determine the effect of cell stage in embryo bisection on the sub-Sequent in vitro and in vivo development in mouse. The embryos of ICR mouse were microsurgicaily bisected at 2-cell, 4-cell, 8-cell, morula and blastocyst stage using a microsurgical blade attached a micromanipulator. These demi-embryos without zona pellucida were cultured up to blastocyst stage and transferred to pseudopregnant mice, and the development of these demi-embryos was compared with the results of intact embryos of the corresponding cell stage. The successful rate of mouse embryo bisection at 4-cell stage (59.0%) was significantly (p <0.05) lower than those at 8-cell (75.6%), 2ce11 (80.7%) or morula stage (84.8%), and highest at blastocyst stage (95.7%). When the bisected embryos without any damage from microsurgery were cultured in vitro up to blastocyst,the in vitro de'velopment of demi-embroys bisected at morula to blastocyst was 91.6 to 95.3%, which was similar to the culture result of intact embryos of corresponding stage. However, the in vitro development of demi-em-bryos bisected at 2- to 8-cell stage was signiflcantiy (p <0.05) lower.The post-transfer implantation rate of demi-embryos developed in vitro to eu-blastocyst were 19.6 and 25.4% in demi-embryos bisected at morula and blastocyst stage,respectively and not significantly (P <0.05)different from the result of intact embryos of the same stage. However, the implantation rates of demi-embryos bisected at 2- or 8-cell stage were significantly (P <0.05) lower than the result from the intact embryos of the corresponding stage.

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추진장약 수락시험의 시험단계 통합 연구 (An Integration Study of the Firing Test Stage at the Acceptance Test for the Propelling Charge)

  • 김재갑;김재훈;나태흠;김보균;박노석
    • 한국군사과학기술학회지
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    • 제19권2호
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    • pp.171-176
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    • 2016
  • In this paper, the integration of the firing test stages at the acceptance test of the propelling charge has been studied using statistical analysis on the basis of the test data of K676 and K677 propelling charge for K9 self propelled howitzer. Acceptance test of K676 / K677 propelling charge consists of 3 stages and each stage is charge assessment test, charge uniformity test and charge proving ground test. Many of rounds are fired and much money is spent to prove the charge performance at each stage. The result of t-test shows that there is no difference between charge uniformity and charge proving ground test stage of K676 and K677 propelling charge.

생태효율성(Eco-efficiency)지표 개발을 통한 KTX와 새마을호 열차의 사용단계 환경성 평가에 관한 연구 (A Study on the Environmental Evaluation in Use Stage of KTX and Samaul Train : the Development of Eco-efficiency Indicator)

  • 최용신;천윤영;이건모;김용기
    • 한국철도학회:학술대회논문집
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    • 한국철도학회 2011년도 춘계학술대회 논문집
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    • pp.1313-1320
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    • 2011
  • World Business Council for Sustainable Development(WBCSD) is effort to achieve sustainable development in economic growth, environmental preservation and social development. Being this way, it is essential for developing evaluation tool which quantify to fulfill sustainable development. Eco-efficiency is one of the quantitative tools to evaluate environmental impact and economic aspect. Eco-efficiency, in general term, means creating more value of product or services with less impact to environment. It indicates as environmental impact in denominator and value of product or services in numerator. Eco-efficiency shows how much economic value reveals to unit environmental impact caused by product or service as an indicator. This study aims at developing eco-efficiency indicator of railway industry considering use stage among the entire life cycle stage of KTX and Saemaul train and also, figure out eco-efficiency value through indicator. Therethrough, it is enables to evaluate created value per environmental aspects. Since rail vehicles demands a lot of energy to transport people during use stage, the environmental impact is more significant than other lkfe cycle stages. Therefore, it quantified environmental indicator as CO2 emission and economic indicator as transportation record per a year with an annual income. This study contributes to be used as a tool for quantifying indicator of comparison evaluation in respect of rail vehicle in use stage.

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