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Comparison of γ-aminobutyric acid and isoflavone aglycone contents, to radical scavenging activities of high-protein soybean sprouting by lactic acid fermentation with Lactobacillus brevis (발아 고단백 콩의 Lactobacillus brevis 젖산발효에 의한 가바와 이소플라본 함량 및 라디칼 소거활성의 비교)

  • Hwang, Chung Eun;Haque, Md. Azizul;Lee, Jin Hwan;Joo, Ok Soo;Kim, Su Cheol;Lee, Hee Yul;Um, Bong Sik;Park, Kyung Sook;Cho, Kye Man
    • Food Science and Preservation
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    • v.25 no.1
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    • pp.7-18
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    • 2018
  • In this study, soy-powder yogurt (SPY) with enhanced levels of ${\gamma}$-aminobutyric acid (GABA) and isoflavone aglycone was produced from sprouting high-protein soybeans (HPSs). The fermented steam-HPS sprouts (0 to 4 cm) were fermented (72 h) with Lactobacillus brevis, and the total free amino acids (FAAs) of the formed mixtures were determined to be 79.53, 489.93, 877.55, 780.53, and 979.97 mg/100 mL in the fermented HPS (FHPS), and the fermented steam-HPS with 0 cm (FSHPS-0), 1 cm (FSHPS-1), 2 cm (FSHPS-2), and 4 cm sprouting lengths (FSHPS-4), respectively. The levels of glutamic acid (GA) and GABA were observed to be the highest, 100.31 and 101.60 mg/100 mL, respectively, in the unfermented HPS (UFSHPS-1, 1 cm) and FSHPS-1 sprouts, respectively. Moreover, the total contents of the isoflavone glycoside form decreased proportionally to the increasing total levels of isoflavone aglycones after fermentation in FSHPS-0, FSHPS-1, FSHPS-2, and FSHPS-4. The levels of isoflavone aglycones were detected as 350.34, 289.15, 361.61, 445.05, and $491.25{\mu}g/g$ in FHPS, FSHPS-0, FSHPS-1, FSHPS-2, and FSHPS-4, respectively. While FSHPS-1 exhibited the highest DPPH (63.28%) and ABTS (73.28%) radical scavenging activities, FSHPS-4 contained the highest isoflavone aglycone ratio (81.63%). All in all, the FSHPS-1 mixture prepared in this study exhibited high GABA content and functional prosperity, thereby making it suitable for potential applications in the soy-dairy industry.

Effects of Horticultural Therapy on the Improvement of the Self-Esteem and Sociality of Mentally Retarded Persons (원예치료프로그램의 적용이 정신지체장애인의 자기주장 및 사회성 향상에 미치는 영향)

  • 박민희;차영주;유영원;부희옥;이숙영
    • Korean Journal of Plant Resources
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    • v.17 no.3
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    • pp.339-351
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    • 2004
  • This study was conducted to evaluate the effect of phased horticultural therapy(HT) program on the experience of psychologic therapy and the development of job and social integration in the mentally handicapped persons. In this study, the mentally handicapped persons participated in HT program were chosen in disabled person's welfare institute of Jeonnam. HT program was performed twice a week with 1 hour activity for 4 months. As a results, the mentally handicapped persons participated in HT program showed high interest of horticulture and improvement of self-respect and the high satisfactory degree of HT program. Therefore, the mentally handicapped persons were showed the experience of both physical and mental therapy, improvement of self-esteem scale and sociality in HT program. Also, the application of HT program with continuously interest will be showed high improvement of physical, psychological and sentimental. In the course of this HT program progress, horticultural therapist and social welfare officer were showed the limitation of role. Therefore the leaders of group for successful HT program be required the comprehensive plan of more efficient HT program and induced technique of continuously up-phased improvements in HT program progress.

The Studies of Activity of Retrotransposon(Tos17) according to Tissue Culture Periods in Rice(Oryza sativa L.) (벼 조직배양 기간에 따른 retrotransposon(Tos17)의 활성에 관한 연구)

  • Yang, Hee-Eun;Fang, Yilan;Shin, Young-Boum;Lee, Boung-Jin;Hong, Soon-Kwan
    • Korean Journal of Plant Resources
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    • v.20 no.5
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    • pp.389-397
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    • 2007
  • Using the active-increment of Tos17 copies in the genome of Oryza sativa L., there were many studies about induction and selection of new mutants. This study mainly focuses on the induction for retrotransposon(Tos17) activity in the callus of Ilpumbyeo(Oryza sativa L.) according to varied culture period and condition. The objectives of this study are obtaining various mutants($M_1$) through plant regeneration, identification of the mutation relation with Tos17, and subsequent phenotyping of the mutants($M_2,\;M_3$). A total of 371 $M_1$ mutants was obtained. The degree of Tos17 activity obtained regeneration plants with each different culture period was evaluated by Southern blot analysis. The result showed that control Ilpumbyeo rice has 5 numbers of copies and the band numbers obtained 7, 8, 9.5, 12, 6, 13.5, 17.5 from culture period of 1, 2, 3, 5, 6, 7, 8 month, respectively. In this study, the result showed that most effectual culture period for activity of Tos17 in Ilpumbyeo rice is 5 month. Hereafter, collections and analysis of various recombination plants will act on an important factor in multiplication and preservation of $M_2$ and $M_3$ generation. And an urgent and important subject is a development of screening method for selection of diverse mutants.

Comparative Study of Litsea japonica Leaf and Fruit Extract on the Anti-inflammatory Effects (까마귀쪽나무 열매와 잎의 항염증 효과 비교 연구)

  • Namkoong, Seung;Jang, Seon-A;Sohn, Eun-Hwa;Bak, Jong Phil;Sohn, Eunsoo;Koo, Hyun Jung;Yoon, Weon-Jong;Kwon, Jung-Eun;Jeong, Yong Joon;Meng, Xue;Han, Hyo-Sang;Kang, Se Chan
    • Korean Journal of Plant Resources
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    • v.28 no.2
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    • pp.145-152
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    • 2015
  • The present study aimed to investigate comparative anti-inflammatory effects of Litsea japonica fruit and leaf extract considering the balance of safety and efficacy. Dose response studies were performed to determine the inhibitory effects of 70% EtOH extract of leaf (L70%) on the pro-inflammatory enzymes expression, COX-2/PGE2 and NO/iNOS, and pro-inflammatory cytokines production, IL-$1{\beta}$, IL-6, and TNF-$\alpha$ in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. We also examined comparative effects of 30 and 70% EtOH extract of fruits (F30% and F70%) at low concentration ($10{\mu}g/ml$ ) in the same conditions. L70% at 50 and $100{\mu}g/ml$ showed inhibitory effects on almost all the inflammatory mediators we examined except for COX-2 regulation, but there were no effects at $10{\mu}g/ml$. Since $100{\mu}g/ml$ of L70% have 18.2% cytotoxicity, we compared the effects of fruit extract, F30% and F70% at $10{\mu}g/ml$ on the regulation of NO/iNOS, PGE2, IL-$1{\beta}$, IL-6, and TNF-$\alpha$ and obtained that fruit extacts are more efficacious and safe than leaf. This study suggests that the 30% EtOH fraction of L. japonica fruit could be a good candidate for development as a functional food supplement in the prevention of inflammatory disorders.

Development and Application of Scientific Model Co-construction Program about Image Formation by Convex Lens (볼록렌즈가 상을 만드는 원리에 대한 과학적 모형의 사회적 구성 프로그램 개발 및 적용)

  • Park, Jeongwoo
    • Korean Journal of Optics and Photonics
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    • v.28 no.5
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    • pp.203-212
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    • 2017
  • A scientific model refers to a conceptual system that can describe, explain, and predict a particular physical phenomenon. The co-construction of the scientific model is attracting attention as a new teaching and learning strategy in the field of science education and various studies. The evaluation and modification of models compared with the predicted models of data from the real world is the core of modeling strategy. However, there were only a limited data provided by the teacher in many studies of modeling comparing the students' predictions of their own models. Most of the students were not given the opportunity to evaluate the suitability of the model with the data in the real world. The purpose of this study was to develop a scientific model co-construction program that can evaluate the model by directly comparing the predicted models with the observed data from the real world. Through a collaborative discussion between teachers and researchers for 6 months, a 5-session scientific model co-construction program on the subject 'image formation by convex lenses' for second grade middle school students was developed. Eighty (80) students in 3 classes and a science teacher with 20 years of service from general public co-educational middle school in Gyeonggi-do participated in this 2-week program. After the class, students were asked about the helpfulness and difficulty of the class, and whether they would like to recommend this class to a friend. After the class, 95.8% of the students constructed the scientific model more than the model using the construction rule. Students had difficulties to identify principles or understand their friends, but the result showed that they could understand through model evaluation experiment. 92.5% of the students said that they would be more than willing to recommend this program to their friends. It is expected that the developed program will be applied to the school and contribute to the improvement of students' modeling ability and co-construction ability.

One dimensional diffusion of NaCl in flooded soil systems (담수(湛水) 토양계(土壤系)에서 염분(鹽分)의 일차원적(一次元的) 확산(擴散))

  • Oh, Yong-Taeg;Yoo, Sun-Ho;Jung, Yeong-Sang;Hong, Chong Woon;Park, Chun Suh
    • Korean Journal of Soil Science and Fertilizer
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    • v.9 no.1
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    • pp.1-8
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    • 1976
  • 1. Under an asumption that Ficks diffusion equation could be applicable in soil systems, the diffusivities of NaCl in several flooded soil systems were measured to range from $0.4{\times}10^{-5}cm^2sec^{-1}$ to $0.83{\times}10^{-5}cm^2sec^{-1}$ 2. It was discussed that, when a polder soil with a uniform initial salt content through the profile is desalinated only by diffusion to flooding water, the salt content in profile is a function of soil depth, diffusion time, and diffusivity as following $$C=C^{\circ}erf\frac{x}{\sqrt[2]{Dt}}$$ 3. On the basis of Kirkham, et al's integration of complementary erra function, the speed of desalting was discussed to be inversely proportional to the square root of time as following $$dq/dt=C^{\circ}{\sqrt{D/{\pi}t}}$$ 4. It was estimated enough to exchange the flooding water once or twice, even when desalination of polder soil is carried out only by diffusion, if the desalination begins in June, the used flooding water is fresh water, and flooding depth is 10cm. 5. Desalination of polder soil by diffusion requires 2 month for good standing of planted rice.

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The influence of herbicides on soil microflora -Influence of butachlor- (제초제(除草劑)가 토양미생물(土壤微生物) Flora에 미치는 영향 - Butachlor 제(第)의 영향 -)

  • Kim, Jung Je;Jung, Hyeon Sig
    • Korean Journal of Soil Science and Fertilizer
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    • v.9 no.1
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    • pp.25-31
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    • 1976
  • The purpose of this investigation is to find out the effects on the changes of microflora and the effects on the inorganic nutrients of the fertilized soil and the non-fertilized soil under submerged condition with the treatment of butachlor at the levels of 250, 500, and 1000ppm respectively. The survey was made within the period of 72 days with 7-day intervals under the incubated condition. The result came out to be the following: 1. The Fluctuated changes of soil microflora A. Bacteria: The plots treated by 500ppm of butachlor in case of non-fertilized soil showed the decreasing tendency of bacteria until 21st day. But there were no effects on the other plots. The plots treated by 250ppm and 500ppm in case of fertilized soil showed decreasing tendency on the 7th day, and 1000ppm until 21st days. B. Actinomycetes: There was no effects on the actinotnycetes in case of the non-fertilized soil but the fertilized soil showed some decrease. In both cases, actinomycetes have generally shown a little increase according to the time passage. C. Fungi: In both cases of the fertilized soil and the non-fertilized soil, the plots treated by the media showed some decreasing tendency in comparison with the control plots. There was no change to the number of fungi according to the time passage. 2. The fluctuated changes of nutrient A. $NH_4-N$: The whole plots showed decrease of $NH_4-N$ by adding butachlor. The higher the intencity of butachiar showed the more decreasing tendency of $NH_4-N$. In case of the non-fertilized soil, the highest increase of $NH_4-N$ appeared from the 7th day to 14th day, but showed degrease thereafter. The increasing tendency was seen in case of the fertilized soil plots. B. $NO_3-N$: Decrease of $NO_3-N$ was shown in the whole plots by the treatment of media, and on the 44th day of cultivation almost none of $NO_3-N$ was detected. C. $NO_2-N$: Whole plots showed the number of $NO_2-N$ highest on the 35th day, and there were nothing measurable on the 44th day. D. Eh: On the fertilized soil, the condition of reduction went on strongly but on the non-fertilized soil, the condition of reduction kept on till 42nd day and oxidation appeared thereafter.

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Effects of the Water Temperature Differences on Rice Growth in a Paddy Field (수온차이(水溫差異)가 수도생육(水稻生育)에 미치는 영향(影響))

  • Kim, Lee-Yul;Jo, In-Sang;Kim, Heung-Bae;Lee, Yong-Hwan;Cho, Byong-Ok
    • Korean Journal of Soil Science and Fertilizer
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    • v.18 no.4
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    • pp.359-365
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    • 1985
  • The four rice varieties, Kwanak, Nongbaek, Pungsan and Nampung-byo were cultivated to examine the growth conditions and grain yield in a Gyuam SiL paddy field irrigated with cold water around $17^{\circ}C$. Water temperatures for various distances from the inlet were measured. The results were summerized as follows. 1. Culm length, panicle exertion, diameter of the 3rd internode stem, heading date, fertilization rate, ripeness rate, no. of grains per panicle and grain yield were sensitive to water temperature. Panicle length, flag-leaf length, diameter of spike-neck and no. of panicles, however, were negligibly sensitive and there were no differences among varieties. 2. Elongations of the 2nd and 3rd internode steam were unsensitive to water temperature. 1st internode elongation and 4th internode development, however, were sensitive which was major factor in the culm length. 3. Ratios of partial dry weight to total dry weight were closely correlated with water temperature, Therefore, dry weight of grain was increased with water temperature while that of plant and root decreased. 4. Chlorophyll contents were decreased with the increment of water temperature and the highest at $20^{\circ}C$. 5. There was no grain yield at $17^{\circ}C$, Increases of grain yield to water temperature per unit were order at Pungsan > Kwanak > Nongbaek > Nampung. 6. The critical temperature in grain yield was $21^{\circ}C$. Optimum temperatures of Japonica ${\times}$ Indica types were higher than those of Japonica types.

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Biogas Production from Agricultural Wastes and Residues in Tropical Region (열대지역(熱帶地域)에서 농산폐유기물(農産廢有機物)을 원료(原料)로한 멘탄가스발생(發生))

  • Joo, Yeong-Hee;Jeon, Yong-Woon;Calilung, Edwin J.;Elepano, Arnold R.
    • Korean Journal of Soil Science and Fertilizer
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    • v.18 no.4
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    • pp.325-335
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    • 1985
  • Biogas production from agricultural wastes were summarized as follows: 1. Biogas Generation Characteristics of Various Manures and Residues a. Gas yield from crop residues like rice straw, rice hull, corn stalk and coconut husk can be improved by addition of animal manures. b. Gas yield from coconut husk can be improved through aerobic fermentation for at least one week before loading in the digester. c. Gas yield from fresh rice straw is better than from pre-fermented one, whether alone or in combination with animal manures. d. Initial study has shown that fresh azolla can be substituted for animal manures in manurerice straw combinations and gas yield derived based on unit volatile solids loaded is actually better than for manure-residue combinations. e. Gas production is highly sensitive to substrate pH and becomes almost nil at a pH of below 6. 2. Effect of ambient conditions and other factors on biogas production in a house hold-size digester. a. Results showed that compaction of rice straw in straw-manure combination can reduce gas yield compared with loosely mixed straw. b. The effective gas production period extended to 70 days using freshly threshed rice straw and fresh cattle manure as feed material. c. Underground and above ground digesters with shade have relatively more stable substrate temperature than aboveground exposed digesters. This relative temperature instability may likely be the reason for lower gas yield for the exposed aboveground digester loaded with loose straw-cattle manure substrate, compared with the underground digester with the same substrate. 3. Economic Analysis a. Based on prevailing costs of fuel, materials, and labor in the Philippines, biogas produced from the household size system is cheaper than either LPG or kerosene. b. If other benefits like organic fertilizer, pollution control and convenience are considered, biogas will surely be the best alternative fuel source.

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Studies on the Development of Acid Tolerant and Superior Nitrogen Fixation Symbionts for Pasture on Hilly Land -II. Selection of Acid Tolerant R. meliloti in virto and Inoculation Effect in Soils (야산(野山) 목초지용(牧草地用) 내산성(耐酸性) 우수(優秀) 질소고정균주(窒素固定菌株) 개발(開發) -II. 내산성(耐酸性) R. meliloti 의 기내선발(器內選拔) 및 토양(土壤) 접종효과(接種效果))

  • Kang, Ui-Gum;Choi, Ju-Hyeon;Cho, Kang-Jin;Jung, Yeun-Tae;Cho, Moo-Je
    • Korean Journal of Soil Science and Fertilizer
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    • v.22 no.1
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    • pp.72-77
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    • 1989
  • A study was conducted to obtain acid tolerant and superior Rhizobium meliloti strain for alfalfa on hilly acid soils. With host plant, eight isolates of R, meliloti selected in the vicinity of Milyang were evaluated for their ability to establish symbiotic effectiveness in acidified tube culture medium and vermiculite pot with different urea levels. Among isolates "YA03" was characterized for the ability to manifest to acid tolerance in three different soils of which pH were 5.0, 6.0, and 7.5. The results obtained were summarized as follows: 1. Of eight isolates "YA03" and "YA09" performed nodule in the tube medium of pH5.0, and in the symbiotic effectiveness YA03 was superior to others. 2. Alfalfa growth and $N_2-fixing$ activity by the inoculation of "YA03" isolate were better than others at the level of urea 1.25mM as well as nonurea. 3. Application of urea with inoculation of "YA03" islate to alfalfa was one of the effective factors for symbiotic effectiveness. 4. In infertile soil of pH5.0 inoculation of R. meliloti "YA03" to alfalfa caused the increase of shoot dry matter of 320% as compared to the control, and the total amount of yield was 131% as much yield in moderate fertile soil of pH7.5. Finally R. meliloti "YA03" isolate was selected as an acid tolerant strain.

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