• Title/Summary/Keyword: Deuk-shin Kim

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Pollen morphology of Paeonia lactiflora Pallas and its germination (작약화분(芍藥花粉)의 형태(形態) 및 발아(發芽))

  • Kim, Jae-Chul;Shin, Jong-Hee;Kim, Jung-Hye;Park, So-Deuk
    • Korean Journal of Medicinal Crop Science
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    • v.4 no.3
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    • pp.231-235
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    • 1996
  • The experiment was conducted to determine pollen morphology of Chinese peony and its germination. The results were obtained as following ; Pollen shpae was usually large ellipse and the pol­len germination rate of Eui-seong Peony line was 71% which was lower than that of other Peony lines. In a bud, the pollen began to be observed on $7{\sim}9days$ before flowering and as getting on for flowering time, normal pollen and germination rates were higher. The pollen germination and elongation beganat 30 minutes after incubation on artificial medium and were completed after $2{\sim}3\;hours$. As the storage time went on, the pollen life span was shortened. The germination rate was 51% at 8 days-storage of room temperature. The germination rate was 48% ad 43% at 95 day-storage in $0^{\circ}C$ and $-15^{\circ}C$, respectively.

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Changes of Root Yield and Paeoniflorin Content affected by Harvesting Times in Peony(Paeonia lactiflora) (작약(芍藥) 수확시기(收獲時期)에 따른 수량(收量) 및 Paeoniflorin 함량(含量)의 변화(變化))

  • Kim, Ki-Jae;Park, So-Deuk;Park, Chun-Hong;Shin, Jong-Hee;Kim, Jae-Cheol;Choi, Boo-Sull
    • Korean Journal of Medicinal Crop Science
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    • v.8 no.1
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    • pp.58-63
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    • 2000
  • Changes of yield and paeoniflorin content according to chemical control and harvest time were investigated. Peonies were harvested after four years from planting. Disease and pest control method was applied normally in first year. From second year to forth year we set two blocks and controlled them differently. In one, peonies were withered early under non-control condition. In the other which was controlled by spraying chemicals four time a year, top part withering was delayed. Harvest was taken four times top part withering was delayed. Harvest was taken four times: Jul. 25, Aug. 25, Sep. 25, and Oct. 25. In non-chemical, at harvest of Jul. 25, paeoniflorin content was highest and dry root weight was 1,126kg/10a which was little less than 1,177kg/10a of Aug. 25. In chemical control, dry root yield of Oct. 25. showed an increase of 33% over that of Jul. But paeoniflorin content in root at Oct. was lower than at Jul. or Aug. For high qualities and harvest yields of peony root, chemical control is necessary to maintain top part to late in Oct.

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Evaluation of Drying Properties and Yields of Domestic Quercus Species for Enhancing Utilization (국산 참나무류의 이용활성화를 위한 건조특성 및 가공수율 평가)

  • Chang, Yoon-Seong;Shin, Hyun-Kyeong;Kim, Sejong;Han, Yeonjung;Kim, Min-Ji;Eom, Chang-Deuk;Lee, Young-Geun;Shim, Kug-Bo
    • Journal of the Korean Wood Science and Technology
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    • v.45 no.5
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    • pp.622-628
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    • 2017
  • In order to develop value-added utilization technology of domestic oaks the processing yield rate from the standing tree to the flooring material for the Quercus variabilis and Quercus mongolica were investigated. Also, to reduce drying time, improved drying schedule was applied and the physical properties were measured. The drying time was 173 hours in total, and the final moisture content was 5.39% (Quercus variabilis) and 4.17% (Quercus mongolica). The color difference of oak lumber before and after drying showed a significant change as ${\Delta}E$ value from 7 to 11. The shrinkage rates of Quercus variabilis and Quercus mongolica during drying were 8.1% and 8.5% in the tangential direction and 5.0% and 6.2% in the radial direction, respectively. The lumber manufacturing yield rate of sawn lumber was 30 to 40% and that of the final product was 8 to 15%. It is expected that the processing characteristics of the Quercus species investigated in this research could contribute to formulate an efficient production plan of domestic hardwoods that are still under utilization.

Effect of Planting Depths on the Growth, Quality and Yield of Oriental Melon (Cucumis melo L. var. makuwa Mak.) (정식깊이가 참외 생육, 품질 및 수량에 미치는 영향)

  • Shin Yong Seub;Kim Jwoo Hwan;Park So Deuk;Park Jong Wook;Kang Chan Koo;Kim Byung Soo;Khan Z.
    • Journal of Bio-Environment Control
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    • v.14 no.1
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    • pp.46-51
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    • 2005
  • This experiment was conducted to investigate planting depth, which applied the basic data for planting growth ability and mechanical planting of oriental melon. 'Gumssaragi-eunchun' variety was approach grafting to 'Shintozoa' and seedling was growing during 45 days in the 9cm pot and then planted on Jan. 16. The comparison of planting plots was carried out for four experimental plots, which were separated into 0cm, 4.5cm, 9cm, standard cultivation, and 12cm planting depth in soil. In the tunnel of vinyl house, the lowest and the highest temperature was $9.3^{\circ}C\;and\;41.2^{\circ}C$, respectively, and humidity was $59\~99\%$ during Jan. 18 and 19. The faster graft-take rate after planting was the deeper planting depth. The growth of 40 days after planting was not significant in each planting plot, except 0cm experimental plot. Fruit weight was the heaviest in 4.5cm planting depth of experimental plot but sugar content and flesh thickness were not significant in each planting depth. The more depth in planting meant the more increase in fermented fruit rate and decrease in marketable fruit rate. Marketable yield was 2,361kg per l0a in 9cm planting plot, which was $2\%\;and\;1\%$ lower than in 0cm and 4.5cm, respectively, and $11\%$ greater than in 12cm planting plot.

Effect of Environment on Plant Growth of Oriental Melon in South-North Directed Ridges under East-West Oriented Vinyl house ($\cdot$서동 하우스내 이랑위치별 환경과 참외 생육)

  • Shin Yong Seub;Park So Deuk;Kim Jwoo Hwan;Seo Young Jin;Kim Byung Soo
    • Journal of Bio-Environment Control
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    • v.14 no.1
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    • pp.15-21
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    • 2005
  • This experiment was carried out to investigate the effect of ridge direction (south-north) on temperature and light intensity on early growth of oriental melon under east-west oriented vinyl house cultivation. The air-temperature of minimum between north and south-ridge in the tunnel of vinyl house was $12.5^[\circ}C\;and\;11.3^{\circ}C$ and that of maximum between north and south-ridge was $36.7^{\circ}C\;and\;34.7^{\circ}C$, respectively. The minimum and maximum air-temperature of north-ridge in the tunnel of vinyl house on Feb. 15 were $12^{\circ}C\;and\;2.0^{\circ}C$ higher than those of south-ridge, respectively. The intensity of daylight between south and north-ridge was similar during 9:00-10:30 a.m., that of south-ridge was higher than north-ridge during 10:30-11:30 a.m. and that of north-ridge was higher than south-ridge during 11:30-17:30. The plant growth after 55 days of planting on the north-ridge was prominent cultivation compared to south-ridge. The female flowering and first harvesting day were earlier in north-ridge than in south ridge. The marketable fruits rate and yields (kg/10a) were $6.7\%$ and 218kg higher in north-ridge cultivation than south-ridge, respectively. Differences of marketable fruit rate and yield (kg/10a) in each ridge were significant.

The Utility of Emergency Ultrasound for Diagnosing Wrist and Ankle Injuries (손목 관절과 발목 관절 손상 환자의 진단에 있어 응급 초음파의 유용성)

  • Lee, Sung Sil;Kim, Dong Un;Park, Deuk Hyun;Cho, Hyun Young;Ahn, Seung Jun;Kho, Chan Young;Shin, Tae Yong;Kim, Young Sik;Ha, Young Rock
    • Journal of Trauma and Injury
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    • v.20 no.2
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    • pp.130-137
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    • 2007
  • Purpose: Ultrasound is of proven accuracy in abdominal and thoracic trauma and may be useful for diagnosing extremity injury in situations where radiography is not available, such as disasters and military and space applications. However, the diagnosis of fractures is suggested by history and physical examination and is typically confirmed with radiography. As a alternative to radiography, we prospectively evaluated the utility of extremity ultrasound performed by trained residents of emergency medicine (EM) one patient with wrist and ankle extremity injuries. Methods: Initially, residents of EM performed physical examinations for fractures. The emergency ultrasound (EM US) was performed by trained residents, who used a portable ultrasound device with a 10- to 5-MHz linear transducer, on suspected patients before radiography examination. The results of emergency ultrasound and radiography and the final diagnosis were recorded, and correlation among them were determined by using Kappa s test Results: Thirty-nine patients were enrolled in our study. The average age was $36.6\;{\pm}\;19.3$ years. There were radius Fx. (n=21), radius-ulna Fx. (n=1), ulna Fx. (n=1), and contusion (n=2) injuries among the wrist injury and lat.-med. malleolar Fx. (n=13), lat. malleolar Fx. (n=6), and med. malleolar Fx. (n=3) injuries among the ankle injury. Comparing EM US with radiography, we found the sensitivity, specificity, positive predictive value, and negative predictive value of EM US for Fx. diagnosis to be 100%, 66.7%, 97.3%, 100% and those of radiography to be 97.2%, 100%, 100%, and 75%, respectively. Kappa s test for a correlation between the Fx. diagnosis of EM US and the final diagnosis of Fx was performed, and Kappa's value was 0.787 (P = 0.004).Conclusion: EM US for Fx. can be performed quickly and accurately by EM residents with excellent accuracy in remote locations such as disaster areas and in military and aerospace applications. EM US was as useful as radiography in our study and had a high correlation to the final diagnosis of Fx. Therefore, ultrasound should performed on patients with extremity injury to determine whether extremity evaluation should be added to the FAST (focused abdominal sonography trauma) examination.

Case-control Study : Cerebral Blood Flow as Measured by TCD in Patients with Risk Factors for Stroke (TCD를 이용한 정상군과 중풍원인질환군의 혈류측정에 관한 비교연구)

  • Heo, Jeong-Eun;Kwon, Jung-Nam;Shin, Won-Tak;Kim, Jong-Deuk;Lee, Sang-Hee;Son, Yeoun-Hui;Kim, Young-Kyun
    • The Journal of Internal Korean Medicine
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    • v.28 no.2
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    • pp.250-261
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    • 2007
  • Objectives : The purpose of this study was to evaluate the blood flow using doppler ultrasound of the MCA, ACA, PCA, BA, ICA in the patients with risk factor for stroke. Methods : 110 patients with risk factor for stroke were selected who had hypertension, diabetes mellitus, hyperlipidemia, or heart disease, as well as 89 healthy adults who did not have any symptoms of those diseases. To evaluate the blood flow, the Vs and Vm of the MCA, ACA, PCA, BA, and ICA in the two groups were measured. Result : In normal healthy adults, subjects showed a decrease in the Vs and Vm: with advancing in age, there was a significant difference in the Vs of MCA, PCA, BA, and ICA. There was a significant difference in the Vm of MCA, PCA, BA, and ICA. In normal healthy adults, females showed high velocities in all examined vessels. There was a significant difference in the Vs of BA. There was a significant difference in the Vm of BA, and ICA. There was a decrease in the Vs, and Vm of all examined vessels of patients with risk factors for stroke in comparison with normal healthy adults. There was no significant difference in the Vs. There was a significant difference in the Vm of MCA, ACA, and PCA. Results were the same between patients aged under 50 and total patients in the Vs and Vm of examined vessels. There were differences between patients aged over 50 and total patients in the Vs of MCA, PCA, BA and for patients aged over 50; patients with risk factor for stroke who had hypertension, diabetes mellitus, hyperlipidemia, or heat disease has higher Vs than healthy adults. Conclusions : There was a significant difference in the blood flow velocity between patients with risk factors for stroke and healthy adults. However, this result was different from results of comparison of TCD findings between patients and healthy individuals by age. Therefore, more detailed studies about aged patients are needed.

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Cloning and Sequence Analysis of the Full-length cDNA of Coxsackievirus B3 Isolated in Korea (한국에서 분리된 콕사키 바이러스 B3 cDNA의 클로닝 및 전체 염기서열 분석)

  • Chung, Yoon-Seok;Kim, Ki-Soon;Park, Jeong-Koo;Lee, Yoon-Sung;Shin, Soo-Youn;Cheon, Doo-Seong;Jee, Young-Mee;Kim, Moon-Bo;Na, Byoung-Kuk;Yoon, Jae-Deuk;Lee, Kwang-Ho;Song, Chul-Yong
    • The Journal of Korean Society of Virology
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    • v.30 no.1
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    • pp.71-81
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    • 2000
  • We have determined and analyzed the full-length cDNA sequence of a coxsackievirus B3 (CVB3) Korean isolate (CVB3-Korea/97) which has been known as a general human pathogen. The whole genome contains 7,400 nucleotides and has a single large open reading frame with 6,555 nucleotides that encodes a potential polyprotein precursor of 2,185 amino acids. The genome also contains a 5' non-coding region (NCR) of 741 bases and a 3' NCR of 104 bases followed by poly(A) tail. Sequence homologies of nucleotides and deduced amino acids between the CVB3-Korea/97 strain and the prototype (Nancy strain) were 81.7% and 91.5%, respectively. The genes encoding the functional proteins including viral protease and RNA dependent RNA polymerase showed higher homology than those encoding the structural proteins. We have further analyzed the sequences of 5' NCR, VP1 and VP2 of CVB3-Korea/97, which are known as cardiovirulent determining factors at the nucleotide and amino acid levels. Although the CVB 3-Korea/97 strain was isolated from an aseptic meningitis patient without cardiomyopathy, its 234th nucleotide and 165th amino acid were uracil and Asn as same as those of other cardiovirulent strains one. However, the 155th amino acid of VP1, which closely associated with cardiovirulence, was replaced with $Arg^{155}$ by single nucleotide substitution from $A^{2916}$ to $T^{2916}$. Moreover, additional amino acid substitutions were observed in the flanking region of $Asp^{155}$. Taken together, amino acid(s) substitution in VP1 may playa critical role in determining cardiovirulence of the CVB3-Korea/97 strain rather than individual nucleotide replacements in the 5' NCR and/or an amino acid substitution in VP2.

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The Content and Risk Assessment of Heavy Metals in Herbal Medicines used for Food and Drug (식약공용 농·임산물의 유해중금속 분석 및 위해도 평가)

  • Cho, In-Soon;Kim, Su-Jin;Park, Ae-Suk;Kim, Jin-Ah;Jang, Jung-Im;Lee, Sung-Deuk;Yu, In-Sil;Shin, Yong-Seung
    • Journal of Food Hygiene and Safety
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    • v.35 no.4
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    • pp.354-364
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    • 2020
  • For this paper we analyzed heavy metals including lead, cadmium, arsenic and mercury, and conducted risk assessment on 171 types of herbal medicines used as foods and drugs distributed in Seoul's Yangnyeongsi and Kyungdong (Gyeongdong) herbal medicine markets. The concentrations of the heavy metals were measured by the ICP-MS and a mercury analyzer. The detection ranges of the lead, cadmium, arsenic and mercury were found to be ND-4.719 mg/kg, ND-1.019 mg/kg, 0.002-8.696 mg/kg and ND-0.058 mg/kg, respectively. Artemisiae Capillaris Herba exceeded the standards of the MFDS (Ministry of Food and Drug Safety) for cadmium items, and Actinidiae Ramulus et Fulium et Fructus Vermicultus exceeded the standards for arsenic items. The non-carcinogenic risk assessment showed that there were no items exceeding 1 and that they were safe. The risk of carcinogenicity to lead was below 10-6 in all items and was evaluated as safe. The risk of carcinogenicity of arsenic was almost all in the range of 10-4 to 10-6. All %PTWI are considered to be safe below 100.

Isolation and antimicrobial susceptibility of microorganisms from milk samples of healthy postpartum sows (모돈 유즙으로부터 분리한 세균의 분포 및 항균제 감수성 조사)

  • Kim, Seong-Tae;Kim, Seon-Deuk;Park, Jun-Young;Cho, In-Young;Choi, Jong-Song;Jung, Ji-Young;Lee, Ju-Dan;Hur, Tai-Young;Jung, Young-Hun;Choi, Chang-Yong;Shin, Sung-Shik;Son, Chang-Ho;Ok, Ki-Seok;Suh, Guk-Hyun
    • Korean Journal of Veterinary Service
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    • v.34 no.4
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    • pp.361-368
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    • 2011
  • The growth rate of piglets will be decreased and the mortality of piglets will be increased in the postpartum sow with mastitis. The aim of this study was to isolate microorganisms from milk samples found in postpartum sows with suckling piglets and to further investigate antimicrobial susceptibility against isolated bacteria. Milk samples were collected from 567 udders of 42 lactating sows and the isolated bacteria was then identified. Isolated bacteria were tested with 12 antimicrobial agents. Bacteria were isolated from 260 milk samples (64.5%). Staphylococcus spp. were the most common microorganisms (74.5%) isolated from sow milk, followed by Streptococcus hyicus (53.9%), Staphylococcus epidermis (11.8%), Staphylococcus aureus (9.8%), and Staphylococcus haemolyticus (53.9%). In the Staphylococcus spp., Streptococcus pneumoniae (5.1%) was isolated as was Streptococcus sanguinis (3.8%) and Streptococcus ovis (2.9%). Results indicated that bacteria isolated from healthy sow milk were mostly susceptible to cephalothin (88.0%), amikacin (85.3%) and cefoxitin (82.4%), but were resistant to streptomycin (10.0%), kanamycin (33.1%) and tetracycline (19.6%).