• 대한의생명과학회지
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• 제24권4호
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• pp.390-397
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• 2018

For the specific detection of human Parvovirus B19 (HuPaV-B19), we designed ten specific PCR primers from 3,800~4,500 nucleotides of HuPaV-B19 complete genome (NC_000883.2). Seventeen candidate PCR primer sets for specific detecting HuPaV-B19 were constructed. In specific reaction of HuPaV-B19, seventeen PCR primer sets showed specific band, however five PCR primer sets were selected basis of band intensity, amplicon size and location. In non-specific reaction with seven reference viruses, four PCR primer sets showed non-specific band, however one PCR primer set is not. Detection sensitivity of final selective PCR primer set was $100fg/{\mu}L$ for 112 minute, and PCR amplicon is 539 base pairs (bp). In addition, nested PCR primer set was developed, for detection HuPaV-B19 from a low concentration of contaminated samples. Selection of nested PCR primer set was basis of sensitivity and groundwater sample tests. Detection sensitivity of final selective PCR and nested PCR primer sets for the detection of HuPaV-B19 were $100fg/{\mu}L$ and $100ag/{\mu}L$ basis of HuPaV-B19 plasmid, it was able to rapid and highly sensitive detection of HuPaV-B19 than previous reports. We expect developed PCR primer set in this study will used for detection of HuPaV-B19 in various samples.

• 한국소음진동공학회논문집
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• 제15권6호
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• pp.697-705
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• 2005

Vibration-based damage identification method using embedded sensitivity functions is discussed. The theory of embedded sensitivity functions is reviewed and applied to identify damage in a three degree-of-freedom system and a metallic panel. Embedded sensitivity functions are algebraic combinations of measured frequency response functions that reflect changes in the response of mechanical systems when mass, damping or stiffness parameters are changed. By comparing the embedded sensitivity functions with finite difference functions using undamaged and damaged frequency response functions, damage is shown to be properly detected, located and quantified in theory and practice assuming that structures of interest are only damaged in one location. Simulated and experimental results indicate that the technique is most effective when changes to frequency response functions are small to avoid distorsions in the estimated perturbations due to variations in the sensitivity functions.

• 한국방사선학회논문지
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• 제17권6호
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• pp.897-902
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• 2023

본 논문에서는 휴대용 X-ray 검출기의 방사선 고감도 검출에 대한 실현 가능 여부를 확인하기 위한 SiPM의 특성 분석을 목적으로 한다. 휴대용 X-ray 검출기는 환자의 위치에 빠르게 접근하여 실시간 이미지를 획득함으로써 의사들은 신속하게 진단을 수행할 수 있는 장점이 있지만 이러한 이동성은 선량 감지의 어려움을 동반한다. 기존 검출기에서는 SiPM을 X-ray Trigger를 판별하는 단순한 용도로 사용하고 있다. SiPM을 통한 X-ray의 고감도 검출 가능 여부를 확인하기 위해 7종의 SiPM Sensor를 비교 선정하고 특성 분석을 진행하였다. 최종 시험에 사용한 SiPM은 10 nGy 수준의 극저선량 조사 조건에서 Signal 구분이 가능하며, 관전압에 따라 Signal 상승곡선의 기울기가 변하는 것이 확인되었다. X-ray 선량에 따라 SiPM의 signal level 및 지속시간이 변화되는 특성을 이용한 고감도 측정이 가능할 것으로 보인다.

• Journal of the Optical Society of Korea
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• 제9권4호
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• pp.169-172
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• 2005

The phase sensitivity of the coincidence detection in one output port of a Mach-Zehnder interferometer is analysed for twin Fock state inputs. Firstly, the ideal detectors with quantum efficiency of unity are assumed for the detection of the output photons. The sensitivity is found out to be independent of the photon number of input light, which means that the Heisenberg limit cannot be reached in the coincidence detection even with ideal detectors. Secondly, the practical detectors with quantum efficiencies less than unity are discussed.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2001년도 제14회 신호처리 합동 학술대회 논문집
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• pp.389-392
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• 2001

A motion vector sensitivity function for abrupt scene change detection is presented. Proposed function detects a scene change by a static uni-value, not using threshold comparion method in real time and compressed domain. All abrupt scene change was detected.

• Smart Structures and Systems
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• 제21권4호
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• pp.449-467
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• 2018

In this paper, a theoretical and numerical study on bridge simultaneous damage detection procedure for identifying both the system parameters and input excitation mass, are presented. This method is called 'Adjoint Variable Method' which is an iterative gradient-based model updating method based on the dynamic response sensitivity. The main advantage of proposed method is inclusion of an analytical method to augment the accuracy and speed of the solution. Moving mass is a model which takes into account the inertia effects of the vehicle. This interaction model is a time varying system and proposed method is capable of detecting damage in this variable system. Robustness of proposed method is illustrated by correctly detection of the location and extension of predetermined single, multiple and random damages in all ranges of speed and mass ratio of moving vehicle. A comparison study of common sensitivity and proposed method confirms its efficiency and performance improvement in sensitivity-based damage detection methods. Various sources of errors including the effects of measurement noise and initial assumption error in stability of method are also discussed.

• 대한의생명과학회지
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• 제25권4호
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• pp.331-338
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• 2019

Human Aichivirus A1 (HuAiV-A1) is a waterborne human pathogenic virus classified as Picornaviridae and Kobuvirus. In this study, we developed a method that can detect about 35 minutes faster with the same detection sensitivity level than the previously reported HuAiV-A1 diagnostic RT-PCR primer. The RT-PCR primer sets developed in this study are capable of detecting HuAiV-A1 at a level of about 100 ag and formed 563 bp amplification product. In addition, the RT-nested PCR method was able to amplify 410 bp using the RT-PCR product as a template. The detection sensitivity of our method was 10 times higher than the method with the highest detection sensitivity to date. Therefore, the detection method of HuAiV-A1 developed in this study is expected to be used in the water environment in which a small amount of virus exists. Also, this detection method is expected to be used as HuAiV-A1 diagnostic technology in both clinical and non-clinical field.

• Structural Engineering and Mechanics
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• 제83권5호
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• pp.593-607
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• 2022

To solve the problem of detecting structural damage, a two-stage method using the Kalman filter and Particle Swarm Optimization (PSO) is proposed. In this method, the first PSO population is enhanced using the Kalman filter method based on dynamic responses. Due to noise in the sensor responses and errors in the damage detection process, the accuracy of the damage detection process is reduced. This method proposes a novel approach for solve this problem by integrating the Kalman filter and sensitivity analysis. In the Kalman filter, an approximate damage equation is considered as the equation of state and the damage detection equation based on sensitivity analysis is considered as the observation equation. The first population of PSO are the random damage scenarios. These damage scenarios are estimated using a step of the Kalman filter. The results of this stage are then used to detect the exact location of the damage and its severity with the PSO algorithm. The efficiency of the proposed method is investigated using three numerical examples: a 31-element planer truss, a 52-element space dome, and a 56-element space truss. In these examples, damage is detected for several scenarios in two states: using the no noise responses and using the noisy responses. The results show that the precision and efficiency of the proposed method are appropriate in structural damage detection.

• Journal of information and communication convergence engineering
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• 제11권3호
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• pp.199-206
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• 2013

An emergency monitoring system for the elderly, which uses acceleration data measured with an accelerometer, angular velocity data measured with a gyroscope, and heart rate measured with an electrocardiogram, is proposed. The proposed fall detection algorithm uses multiple parameter combinations in which all parameters, calculated using tri-axial accelerations and bi-axial angular velocities, are above a certain threshold within a time period. Further, we propose an emergency detection algorithm that monitors the movements of the fallen elderly person, after a fall is detected. The results show that the proposed algorithms can distinguish various types of falls from activities of daily living with 100% sensitivity and 98.75% specificity. In addition, when falls are detected, the emergency detection rate is 100%. This suggests that the presented fall and emergency detection method provides an effective automatic fall detection and emergency alarm system. The proposed algorithms are simple enough to be implemented into an embedded system such as 8051-based microcontroller with 128 kbyte ROM.

• Journal of Microbiology and Biotechnology
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• 제24권7호
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• pp.1004-1007
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• 2014

Culture is the gold standard for diagnosis of tuberculosis, but it takes 6 to 8 weeks to confirm the result. This issue is complemented by the detection method using polymerase chain reaction, which is now widely used in a routine microbiology laboratory. In this study, we evaluated the performance of the Seegene Anyplex TB PCR to assess its diagnostic sensitivity and specificity, and compared its results with the Roche Cobas TaqMan MTB PCR, one of the most widely used assays in the world. Five university hospitals located in the Chungcheong area in South Korea participated in the study. A total of 1,167 respiratory specimens ordered for acid-fast bacilli staining and culture were collected for four months, analyzed via the Seegene Anyplex TB PCR, and its results were compared with the Roche Cobas TaqMan MTB PCR. For detection of Mycobacterium tuberculosis, the diagnostic sensitivity and specificity of the Anyplex TB PCR were 87.5% and 98.2% respectively, whereas those of the Cobas TaqMan were 92.0% and 98.0% respectively (p value > 0.05). For smear-positive specimens, the sensitivity of the Anyplex TB PCR was 95.2%, which was exactly the same as that of the Cobas TaqMan. For smear-negative specimens, the sensitivity of the Anyplex TB PCR was 69.2%, whereas that of the Cobas TaqMan TB PCR was 84.6%. For detection of MTB, the Seegene Anyplex TB PCR showed excellent diagnostic performance, and high sensitivity and specificity, which were comparable to the Roche Cobas TaqMan MTB PCR. In conclusion, the Anyplex TB PCR can be a useful diagnostic tool for the early detection of tuberculosis in clinical laboratories.